Methylation Status and Expression of E-cadherin in Oral Squamous Cell Carcinomas Compared to Benign Oral Epithelial Lesions

Son, Hyun-Jin,Chu, Jung-Youb,Cho, Eui-Sic,Lee, Dong-Geun,Min, Myung-Gee,Lee, Suk-Keun,Cho, Nam-Pyo The Korean Academy of Oral Biology 2006 International Journal of Oral Biology Vol.31 No.2

Expression of invasion/metastasis suppressor, E-cadherin, is reduced in many types of human carcinomas. Although somatic and germline utations in the CDH1, which encodes the human E-cadherin, have frequently been reported in cases with diffuse gastric and lobular breast ancers, irreversible genetic inactivations are rare in other human carcinomas. Recently, it has been well documented that some genes in human cancers may be inactivated by altered CpG methylation. Herein, we determined the expression and methylation status of E-cadherin in oral squamous cell carcinoma (SCC) by immunohistochemistry and methylation-specific PCR. The expression of E-cadherin was significantly higher in the well-differentiated oral SCCs than the moderately or poorly differentiated ones. None of eight tested benign pithelial hyperplasias showed aberrant methylation, whereas five of 12 oral squamous cell carcinomas showed aberrant methylation. When we compared E-cadherin expression with methylation status, oral SCCs with normal methylation showed a higher expression of E-cadherin than those with methylation. These findings suggest that aberrant CpG methylation of CDH1 promoter region is closely associated with transcriptional inactivation and might be involved in tumor progression of the oral mucosa.

IEEE 802.11기반의 애드 혹 네트워크의 효율적인 동시적 전송

공현표,김은성,김두용 순천향대학교 산업기술연구소 2007 순천향 산업기술연구소논문집 Vol.13 No.1

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TSC 기둥-보 접합부의 거동에 관한 실험적 연구

표현석,김성배,김상모,이창남,김상섭,김규석 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1(구조계)

Connection is necessary element of every structure. Connection is important element to increase composition rate of composite beam. Welding joint has been used at composit beam-column connection. Welding joint's quality control is harder than high strength bolt's one. This study based on the experiments of 4 specimens by beam-column connection test. In this paper, we analyze behavior of composite beam-column connection through an experimental study and wish to present application possibility to TSC beam.

새로운 백금착체인 tetrachloro [bis(2-chloroethyl)ethylenediamine-N,N']platinum(IV)과 cisplatin이 마우스의 면역반응에 미치는 영향

표명윤,유경미,최지선,오현정 숙명여자대학교 약학연구소 1993 약학논문집-숙명여자대학교 Vol.9 No.-

Effects of a new platinum complex, tetrachloro-[bis(2-chloroethyl) ethylenediamine-N,N']platinum(IV)[PtCl₄-2(CEen)] and cisplatin on the immune response were investigated. Male ICR mice were treated at the respective LD50(20% or 60% LD50) of cisplatin and PtCl₄-2(CEen). The results were as following : LD50 of PtCl₄-2(CEen) for male ICR mouse was 122.5mg/kg(i.p.) and higher than cisplatin(16.3mg/kg, i.p.). Body-, spleen-, thymus-, liver-weight, number of WBC and RBC were reduced dependently on the dose and day of administeration of cisplatin and PtCl₄-2(CEen). But these toxicities produced by PtC^-^CEen) appeared to be relatively less and to improve faster than by cisplatin. The titers of hemagglutinin and hemolysin to SRBC in mice received cisplatin and PtCl₄-2(CEen) were decreased significantly and independently on the day of antigen injection. Cisplatin and PtCl₄-2(CEen) stimulated significantly contact hypersensitivity to DNFB when administered prior to sensitization(DNFB), but suppressed slightly when administered after sensitization.

치환된 LaFeO_(3)의 환원반응성에 대한 연구

전현표,이상범 배재대학교 자연과학연구소 2004 自然科學論文集 Vol.15 No.1

LaFeO_(3) 복합산화물과 부분 치환한 LaFeO_(3) 복합산화물을 Citrate와 Cyanide 공침법으로 합성하여 공기중에서 850℃로 24시간동안 열처리해 제조방법에 의한 영향을 비교하였다. XRD와 IR의 결과에 의하면, 합성된 산화물은 Orthorhombic의 Perovskite구조를 나타냈으나 B자리에 0.5mol의 Cu가 치환된 복합산화물은 단일상을 얻지 못하였다. 또한, 이들 산화물의 환원반응은 치환하지 않은 LaFeO_(3.17) 복합산화물은 2단계 반응인 반면에 치환한 복합산화물은 3단계 반응으로 Dopant에 의한 새로운 환원단계가 존재함을 알았다. LaFeO_(3) and substituted LaFeO_(3) mixed oxides were prepared by Citrate and Cyanide method in air 850℃/24h. These oxides of orthorhombic perovskite were characterized by XRD and IR, but substituted LaFeO_(3) with 0.5mol Cu at B site was not obtained single phase. Also, reduction reaction of un-substituted LaFeO_(3.17) were two steps but each site substituted oxides were three steps reactions. These means that new reduction step of each site substituted oxides were atributed to dopant.

原電에서 부하추종이 BTRS에 미치는 영향

홍원표,양현수,강 용,강덕원 忠南大學校 産業技術硏究所 1988 산업기술연구논문집 Vol.3 No.1

With the growing need of the country's electrical energy from nuclear power generation, the technical importance on the load following has been widely recognized. The load following is being executed by two modes, Boron Regeneration System(BRS) and Boron Thermal Regeneration System(BTRS). Especially, the function of BTRS is solely relied on the efficient use of Boration/dilution in strongly basic resin bed. Therefore, the most influential factors to be investigated in load following operation is to determine the effect of boron concentration in burn-up reactivity and to find the way how to improve the BTRS in order for reusing all the reactor cooling water contaminated by radioactive wastes. This study is mainly devoted to the following purposes : (1) Evaluation of operation data in nuclear power plant about the relation of burn-up reactivity and boron dilution/ boration in BTRS. (2) Diagnostic suggestion on the solving problems in operation of Demineralizer(exchange resin bed).

유전자 검색을 위한 DNA 칩 제작용 microarrayer의 개발

이현동,김기대,김찬수,임용표,박정규 충남대학교 농업과학연구소 2003 농업과학연구 Vol.30 No.1

외국의 경우 게놈 연구 및 바이오 산업에 DNA 칩을 제작할 수 있는 로봇 시스템을 싼 가격에 사용하고 있으나 우리나라의 경우 자동화 시스템을 비싼 가격에 외국에서 도입하여 사용하기 때문에 바이오 산업 및 연구 분야에서의 생산비를 높이게 돼 국내외적으로 생명공학의 경쟁력을 저하시키는 원인이 된다. 따라서, 본 연구에서는 유전체 연구에 필수적인 DNA 칩 제작을 위한 연구용 pin 타입 microarrayer를 개발하였으며, 그 구체적인 연구결과는 다음과 같다. 1. 본 연구에서는 DNA칩 제작을 위한 연구용 pin 타입 microarrayer를 개발하였으며 3축 직교좌표형 로봇 본체 DNA를 묻혀 silylated 슬라이드에 점착하는 DNA 점착 헤드, 칩 및 웰 플레이트 고정부, 핀을 세척 및 건조하는 세척 및 건조장치 등으로 시스템을 구성하였다. 2. DNA 점착 헤드는 DNA 점착시 제도용 펜촉을 사용하도록 설계, 제작하였으며, 슬라이드에 DNA를 점착할 때는 핀이 일정한 힘으로 슬라이드를 누르며 점착할 수 있도록 자석의 반발력을 이용하였다. 3. DNA 점착 헤드 핀의 세척을 위하여 증류수 분사 및 진동 브러쉬를 이용하였으며 세척실험 결과, 핀을 1㎜/s로 이동시키며 브러쉬를 통과하도록 하는 방법이 세척효과가 높은 것으로 나타났으며, 핀 건조실험결과는 8.5㎏/㎤의 압축공기를 30초 동안 핀에 분사하였을 때 핀이 건조되는 것으로 나타났다. 4. 본 로봇 시스템을 이용하여 DNA를 12장의 슬라이드에 모두 점착시키기 위하여 웰 플레이트에서 핀이 DNA를 묻히는 실험을 실시한 결과, 10초 이상 핀에 DNA를 묻혔을 때 슬라이드 12장을 모두 찍는 것으로 나타났으며, 슬라이드에 핀이 1초간 접촉할 때의 DNA 스팟의 크기는 평균 280㎛가 되는 것으로 나타났다. 최소 점 간격을 0.32㎜로 설정한 후 DNA를 점착해 본 결과 최대 8,100여 점의 DNA 스팟을 한 슬라이드에 점착할 수 있는 것으로 나타났다. 5. 본 로봇 시스템은 12장의 동일 DNA 칩을 생성하기 위해 핀의 세척, 건조, DNA를 묻히는 과정 및 DNA 점착 등의 한 과정을 2분 50초 동안 수행할 수 있는 것으로 나타났다. This study exploits the robot system which is necessary in gene study, bio-technology industry. As well, it can achieve the job of DNA chip manufacturing whose use rate has been increased recently. The robot consists of DNA spotting device for spotting DNA on the silylated slide and well plate, bed for fixing well-plate, washing & drying device of washing and drying the pin part of DNA spotting device, distillation-water vessel, and discharge vessel of wash water. We made the term of sticking DNA to the pin on well plate to be 15 seconds. The spot size of DNA was set to be 0.28㎜ on the average by bringing the slide into contact with pin for 1 second. At this rate, if DNA is spotted in the minimum space possible of about 0.32㎜, it can stick about 8,100 DNA spots on the well plate. Analyzing the procedure: Movement starts. Pin washes, dries, and smears DNA on the well plate. Spots DNA onto 12 chips takes 2 minutes and 50 seconds.

평면 2자유도 모델을 이용한 리니어 모터로 구동되는 다축 마이크로 밀링머신 크로스헤드의 진동해석

장성현,황현영,정용민,최영휴,홍정표 한국공작기계학회 2008 한국공작기계학회 추계학술대회논문집 Vol.2008 No.-

In this paper, a simple 2-D.O.F. planar motion model is proposed in order to analyze the snake motion of a cross head assembly of a Multi-axis micro milling machine (M4) for BT-factory, that is travelling on linear guide. In the proposed mathematical model, the friction between head and guide ways is neglected, and also the support structures including the linear guides of M4 are assumed to be rigid. The equations of motion of the proposed model are derived and successfully solved to determine vibration responses of a cross head assembly due to some applied traction forces.

Al-Cu-Mg合金의 時效析出에 미치는 豫備時效의 影響

趙顯麒,洪俊杓,全在英 경북대학교 공과대학 1983 工大硏究誌 Vol.12 No.-

The influences of preageing on age-precipitation in Al-2.02t% Cu-1.1wt% Mg alloy have been investigated by means of measurements of hardness, tensile strength and electrical resistivity and transmission electron microscopy. The results obtained are summarized as follows. The two-step aged alloy is more age-hardenable than the onestep aged alloy in early stage of ageing. It is because of the formation and growth of solute atom cluster and GPB zones. The maximum hardness and strength of the alloy aged 200℃ is obtained by preaged at 100℃. It is because of fine and dense distribution of S'metastable phase. The hardness and strength are remarkably decreased with sufficient ageing time after preageing at room temperature and 170℃. It is because of the formation and coarsening of S'metastable phase by the preageing.

탄소섬유 프리프레그 외부 표면 적층방법에 따른 하이브리드 샤프트 진동특성

홍동표,김현식,홍용,고유곤,유청환 한국공작기계학회 2009 한국공작기계학회 춘계학술대회논문집 Vol.2009 No.-

Studies on carbon fiber epoxy composite is being conducted broadly in industries because of its specific stiffness, specific strength and good fatigue resistance. Because carbon fiber epoxy composite has high vibration characteristics, if existing metal material is alternated by carbon fiber epoxy composite, structure will be low weight, satisfy 1st bending natural frequency and have high fatigue resistance. In this study, research on to manufacture hybrid propeller-shaft using carbon fiber epoxy composite instead of current steel propeller shaft of rear wheel drive vehicle. For this, change of 1st bending natural frequency by the number of layers of carbon fiber epoxy composite on the outer surface of aluminum shaft was observed. Compared the change of 1st bending natural frequency by FEA with computer simulation and vibration test. Through this, the amount of increase in 1st bending natural frequency of hybrid shaft by the increase in number of layers of carbon fiber epoxy composite and high vibration characteristics of hybrid shaft was acknowledged.