烏貝散이 흰쥐 胃의 Gastrin, Histamine, Somatostatin 면역반응세포에 미치는 영향

이시섭,나현욱,고병문,이광규,이창현 대한동의병리학회 2001 동의생리병리학회지 Vol.15 No.5

To investigate the elects on the administration of Opae-san in rats. Opae-san (500mg/day) and omeprazole(10mg/day) were administration with stomach tube for 4 weeks and 8 weeks. This experiment were investigated numerical change of immunoreactive cells of gastric, histamine and somatostatin in rat stomach mucosa by the immunohistochemical method. The results were as follows : 1. In Opae-san administration group for 4 weeks, the number of gastric immunoreactive cells were increased in one and a half times than that of control group. In omeprazole administration group for 4 weeks, the number of gastric immunoreactive cells were increased in four times than that of control group. In Opae-san administration group for 8 weeks, the number of gastric immunoreactive cells were increased four times than that of control group. In omeprazole administration group for 8 weeks, the number of gastrin immunoreactive cells were increased in six times than that of control group. 2. In Opae-san administration group for 4 weeks, the number of histamine immunoreactive cells were increased in two times than that of control group. In omeprazole administration group for 4 weeks, the number of histamine immunoreactive cells were increased in six times than that of control group. In Opae-san administration group for 8 weeks, the number of histamine immunoreactive cells were increased three times than that of control group. In omeprazole administration group for 8 week, the number of histamine immunoreactive cells were increased in seven times than that of control group. These results suggest that Opae-san extracts inhibit the secretion of gastric acid and this extract use to therapeutic herb of gastric disorders related to the hyperacidity and gastric ulcer.

사료용 교잡종 옥수수 연구 : Ⅲ. CNU와 SK계통을 이용한 교잡종 옥수수의 건물중 및 종실 수량

이희봉,김동욱,김준표,김용일,정재영,최현구,문현귀,이충열 충남대학교 농업과학연구소 2003 농업과학연구 Vol.30 No.2

공시 교잡종에 대한 각 지역에서 선발된 우수 교잡종의 간장 및 착수고는 대조구와 비슷한 경향이었으며, 생체 및 건물수량은 분얼수 및 간장과 관련이 큰 것으로 나타났으나 재식 밀도와 기상상태와도 밀접한 관계로 나타났다. 따라서 생체중은 대전지역에서 재배된 교잡종 H67이 대조구인 광안옥에 비해 53.2%로 높았고, 수원에서 역시 41.8%으로 높았으며, 종실수량은 H15과 H67에서 대조구보다 11.7%와 9.7%로 각각 높게 나타났다. 대전에서 분얼성인 H42와 무얼성인 H65에서 대조구보다 9.1%, 5.2%증가하였고, 수원에서는 분얼성이 H42와 무얼성인 H65에서 대조구보다 9.1%, 8.5% 증가하여 교잡종간, 지역간에 큰 차이를 보였다. 한편 후기 녹체성은 무얼형보다 분얼형에서 낮은 경향을 보였는데 SK계통과 교배된 조합에서 우수한 편으로 나타나 우수 교잡종 육성시 중요 고려사항으로 지적되었다. In order to develop the crude forage maize hybrids with high dry matter and grain yield, several hybrids evaluated as superior hybrids at three regions in 2001. They were planted and surveyed again for the second selection in 2002. The selected hybrids showed large difference in some major characteristics according to planting areas and dates(Suwon : April 25. Daejeon : May 15 and Milyang : May 25), but insect and disease injury were similar to check. Fresh and dry weight of the H67 hybrid at Suwon and Daejeon was the highest among hybrids including check, but low in Milyang due to raining after flowering. Grain yield was higher than check in H15 at Suwon H45 and H37 at Daejeon and H67 at Milyang.

유색미에 관한 연구 : Ⅰ. 감마선 처리에 의해 유래된 Dohoku 유색미 돌연변이체의 주요 특성 Ⅰ. Characteristics of Dohoku color rice mutants derived by Gamma-ray Mutagenesis

이희봉,최현구,김동욱,김준표,정재영,정종태 충남대학교 농업과학연구소 2002 농업과학연구 Vol.29 No.2

1. 돌연변이체 DM-1과 DM-2의 간장은 양친인 Dohoku나 대구조인 흑진주벼보다 짧은 반면에 출수기는 7일내지 20일가량 늦어졌다. 2. 이들 DM-1과 DM-2의 수장은 대조구와 비슷하였으나 주당 이삭수는 대조구와 같이 DM-2에서 매우 많았다. 3. 선발된 계통들에 대한 주당 영화수는 대조구보다 적었으나 영색은 갈색내지 짙은 자주색으로 다양한 변이를 보였고 까락길이 역시 변이가 컸다. 4. 안토치안 색소에 대한 공시계통간 차이를 optical density(OD_(530))값으로 나타낸 결과 DM-2 변이계통에서 2.23, DM-3 변이계통에서 2.26으로 나타나 대조구인 흑진주벼의 2.59보다 크게 낮았다. Culm length of Dohoku mutant 1(DM 1) and mutant 2(DM 2) were shorter than Dohuko parent and Heukjinjubyo check, while days to heading were delayed seven to 20 days Panicle length of these were similar to check and number of panicle per plant of DM 2 was highly appeared than check. Spikelets per plant of the selected lines were lower than check, and glume color of these were varied from brown to dark purple and awn length was also varied according to line used. Anthocyanin content of each mutant line as measured by 530㎚ were lower than that of check in that OD 2.23 and OD 2.26 for DM 1 and DM 2, respectively, and 2.59 for check.

비대칭 경로를 지원하는 개선된 DSR 라우팅에 관한 연구

이광배,김현욱,곽승욱,박용정 명지대학교 산업기술연구소 2001 産業技術硏究所論文集 Vol.20 No.-

This paper introduces a dynamic source routing protocol supporting asymmetric routes on mobile ad hoc network. For performance evaluation, average route discovery time, average data transfer time, average route recovery time and data reception rate were considered with several scenarios relating to connection pattern, moving scenario, usage of secondary route chche in intermediate nodes and periodic hello message interval. As the results, we found that the data reception rate increased as the node mobility went down and the number of maximum connections between nodes was reduced. In the case that secondary route cache was used in the intermediate nodes, the average data transfer time was decreased because the possibility of route discovery to destination node was increased. In addition, it was found that the hello message interval at 0.1 to 1 second was fairly good.

고전적 순막 조건화 동안의 해마 뇌전도와 다단위 활동

이두현,김현택,류재욱,김기석 한국심리학회 1990 한국심리학회지 생물 및 생리 Vol.2 No.1

해마에서만 발견되는 뇌전도는 낮은 주파수, 높은 진폭의 특징을 갖고 동기화가 잘 이루어져 마치 정현파에 가까운 형태를 보인다. 이러한 뇌전도에 대해 서파리듬 또는 θ파라 부르고 전기 생리학적인 연구가 많이 진행되고 있다. θ파의 주파수 특성, 발생기전, 행동과의 관련성 등에 대해 집중적 연구가 이루어졌고, 그러한 연구를 기초로 θ파의 기능에 관한 여러가설이 도출되었다. 본 연구에서는 이러한 해마의 서파리듬이 행동과 어떤 관계를 갖고 있는지를 알아보기 위하여 토끼의 순막반응을 고전적으로 조건화시키는 동안에 미리 해마에 삽입한 전극으로부터 뇌전도와 다단위활동을 기록하였다. 해마 서파리듬에 대해서는 주파수 분석을 실시하고 다단위 활동은 시간단위당 발화 횟수를 측정하여 조건화과정과의 관련성을 살펴보았다. 실험 결과, 해마의 다단위 활동은 순막의 조건 반응과 높은 상관관계를 보여주었다. 해마 서파리듬은 조건화 절차와 관계없이 조건자극, 무조건자극 제시 기간에 제2유형인 4,5㎐의 감소와 제1유형인 7,8㎐의 증가를 보였다. 이는 해마 서파리듬의 기능이 조건화된 반응보다는 조건자극과 무조건자극의 제시와 관련이 있음을 시사하는 것이었다. 이러한 결과를 해마 θ파의 기능에 관한 여러 가설에 비추어 논의하였다. This study was investigated to hippocampal θ rhythm and multiple unit response during classical conditioning of rabbit's nictitating membrane response. Hippocampal θ rhythm usually was classified into type 1 and type 2. According to various electrophysiological researches, type 1 θ rhythm was highly correlated with voluntary behavior and type 2 θ rhythm with automatic behavior, but there were studies which assert that type 1 θ rhythm was correlated with sensory information processing. So, this study was an attempt to investigate those results as compared paired conditioning group with unpaired control group. Paired group was presented 500msec tone CS followed 100msec air puff US after 400msec interstimulus interval. But unpaired group was presented 500msec tone and 100msec air puff independently. There was no difference in distinction of θ rhythm between the two group. Again, there was no correlation between θ rhythm and conditioned response. But, there was high correlation between multiple unit response and conditioned response. The result indicated that type 1 θ rhythm was highly correlated with presentation of tone and air puff in both group, this result suggest that type 1 θ rhythm is correlated with subject's information processing.

젓갈로부터 유용 박테리오신 생산균주의 탐색

이나경,전송애,하정욱,백현동 경남대학교 환경문제연구소 1998 환경연구 Vol.21 No.-

Bacteriocins are proteins produced by heterogeneous group of bacteria that have a bactericidal effect on closely related organisms. Recently, bacteriocins from lactic acid bacteria(LAB) and other food-related organisms have been the subject of much research of their potential as food biopreservatives. The goal of this study were to isolate LAB from commercial fermented fish products and identify their bacteriocin activity. All bacteriocin-producing isolates were identified as LAB. The NK24, NK34, and SA72 isolates were as identified as Lactococus lactis subsp. Lactis where as SA131 isolate was Lactobacillus brevis according to database of API50 CHL kit. All antimicrobial substance(s) produced from 4 LAB isolates were lost their antibacterial activity completely by treatment of some proteases, which indicate its proteinaceous nature. The bacteriocins produced from NK24, NK34, and SA72 isolate showed broad spectrum of activity when compared to SA131 bacteriocin.

간호사의 직무 스트레스와 이직 간의 관련성

이은희,장세진,김현옥,노재훈,박은정,원종욱 대한산업의학회 2007 대한직업환경의학회지 Vol.19 No.2

무선 애드 혹 기반 액티브네트워크상에서 QoS지원에 관한 연구

李基源,裵振勝,丁燦赫,金玄郁,李光培,姜景仁,朴京培,文泰洙,郭承郁,河載承 明知大學校 産業技術硏究所 2003 産業技術硏究所論文集 Vol.22 No.-

Abstract - In this thesis, we propose a QoS supporting method on wireless ad hoc-based active network for improving reliability. On the loss of route path, the existing DSR routing protocol has several problems with wireless network communication because of decease of data reception rate and increase of network load. The proposed method improves reliability of data transfer by using functions of active network. That is, by using functions of active nodes and active packets, the proposed method supports QoS with reservation transfer method that saves data packets on transfer at intermediate nodes with lost path and then re-transmit the packets after route reconfiguration.

염화비닐 노출 근로자의 시료채취시기에 따른 요중 thiodiglycolic acid의 농도 변화

김현수,김치년,원종욱,차봉석,이경종,노재훈 大韓産業醫學會 2006 대한직업환경의학회지 Vol.18 No.2

목적: 본 연구는 염화비닐 및 폴리염화비닐수지 제조공장의 VCM 노출 근로자들을 대상으로 소변 시료채취 시기에 따른 요중 TDGA 농도의 변화를 알아보고자 하였다. 방법: VCM 및 PVC 제조공장 남성 근로자 31명을 대상으로 공기중 VCM 개인노출평가를 실시하였으며 소변내 TDGA 배설 양상을 알아보기 위해 3일 휴식 후 척 작업 시작 전(TDGA1), 1일 작업종료 후(TDGA2), 1일 작업 한 다음날 작업 전(TDGA3) 소변을 채취하였다. VCM에 노출되지 않은 대조군 30명에 대해 작업종료 후 소변을 채취하였다. 공기 중 VCM의 측정 및 본석은 NIOSH 공정시험법 No. 1007에 따라 실시하였다. 요중 TDGA 분석을 위하여 소변을 trimethylsilyldiazomethane(2.0 M in diethyl ether)으로 메틸화 반응하여 가스크로마트그래피/펄스불꽃광도검출기로 분석하였다. 결과: VCM 노출 후 요중 TDGA가 배설되어 감소하는 시기를 조사하기위해 대조군과 VCM 노출 근로자들의 3일 휴식 후 작업 시작 전 요중 TDGA 농도 (TDGA1)를 비교한 결과 각각 0.179±0.271 mg/g creatinine, 0.218±0.443 mg/g creatinine으로 통계학적으로 유의한 차이가 없었다. 1일 작업 종료 후 요중 TDGA 농도(TDGA2)는 0.434±0.623 mg/g creatinine, 1일 작업 한 다음날 작업전 요중 TDGA 농도(TDGA3)는 0.767±1.056 mg/g creatinine으로 순차적으로 증가하였다. 하루 노출량을 평가하기 위해 공기 중 VCM 농도와 요중 TDGA와의 단순회귀분석 결과 1일 작업한 다음날 작업 전 요중 TDGA 농도(TDGA3)와 공기 중 VCM 농도와의 관련성은 R^(2)=0.4215로 1일 작업종료 후 요중 TDGA 농도(TDGA2)보다 높았다. 결론: VCM 노출 후 3일이 경과하면 대조군의 요중 TDGA 농도 수준으로 감소하여 요중 TDGA의 배설 반감기가 3일 이내인 것으로 판단된다. 또한, 하루 근무하고 그 다음날 작업 전에 채취한 요중 TDGA 농도(TDGA3)가 전날 근무시의 VCM노출량과 상관성이 가장 높아 하루 동안의 VCM노출을 평가하기 위한 유용한 생물학적 지표로 사용할 수 있을 것으로 판단된다. Objectives: The study was performed to investigate the changes of urinary thiodiglycolic acid (TDGA) concentration in workers exposed to vinyl chloride monomer (VCM) according to the time of sampling urine. Methods: The personal exposure to airborne VCM was assessed and urinary TDGA concentration was sampled in 31 workers employed in a VCM and polyvinyl chloride (PVC) manufacturing factory. Urinary TDGA was sampled three times: before the start of the shift after 3 days off (TDGA 1), after the end of the first-day shift (TDGA2) and before starting the following day shift after completing the one- day shift (TDGA3). Urinary TDGA in 30 workers who had not been exposed to airborne VCM was sam- pled after the end of the shift. A gas chromatography/pulsed flame photometric detector (GC/PFPD) was utilized to analyze TDGA concentration in urine after the urine was methylated with trimethylsilyldia- zomethane (2.0M in diethyl ether). Results: The creatinine level was 0.179±0.271 mg/g in the control workers and 0.218±0.443 mg/g in the workers before the start of the shift after 3 days off (TDGA1), showing no significant difference (p=0.7035). Urine samples were compared according to sampling time in order to investigate the change of urinary TDGA concentration in the case of continuous exposure to airborne VCM. In VCM-exposed workers, urinary creatinine concentration was 0.434±0.623 mg/g in TDGA2 and 0.767±1.056 mg/g in TDGA3, which indicated a gradual but significant increase (p=0.024). In terms of the statistical correlation between airborne VCM and urinary TDGA to evaluate exposure dose per day, of the three urinary TDGA concentrations, TDGA3, showed the highest degree of regression (R^(2)=0.4215) with 8h-TWA airborne VCM concentration. Conclusion: Based on this result, the excretion half-life of urinary TDGA was assumed to be less than 3 days, because the concentration of urinary TDGA at 3 days after exposure to airborne VCM was decreased to the level of urinary TDGA concentration in the control workers. The concentration of urinary TDGA increased in the case of continuous shift, due to the accumulation of residual metabolites of TDGA. It was considered that TDGA3 can be applied as a useful biological index to evaluate the exposure dose of airborne VCM during one day because TDGA3 showed the highest correlation with the exposure dose of airborne VCM in the previous shift day.

무선 센서 네트워크를 위한 에너지 인식 하이브리드 클러스터링

한욱표,김승남,이제현,정영준 강원대학교 정보통신연구소 2007 정보통신논문지 Vol.11 No.-

Many studies for the efficient utilization of node energy in wireless sensor networks has been done because sensor nodes operate with limited power based on battery. Existing cluster structure has problem that energy consumption is unbalanced because energy consumption is concentrated in cluster head. And in case distribution density of sensor node is low, the energy efficiency is declined because only a few members exist in cluster. In this paper, we submit an hybrid clustering mechanism to construct topology by mixing cluster structure and mesh structure selectively according to distribution of sensor nodes to improve energy efficiency. And we attempt to derive balanced energy consumption by selecting cluster head considering residual energy of each node. The performance of the proposed mechanism has been examined and evaluated with the NS-2 simulator in terms of network lifetime and end-to-end delay.