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Yeo, Su-Min,Park, Nam-Mee,Song, Hong-Gyu,Choi, Hyoung-T. The Microbiological Society of Korea 2007 The journal of microbiology Vol.45 No.3
Trametes versicolor has a lignin degrading enzyme system, which is also involved in the degradation of diverse recalcitrant compounds. Manganese-dependent peroxidase (MnP) is one of the lignin degrading enzymes in T. versicolor. In this study, a cDNA clone of a putative MnP-coding gene was cloned and transferred into an expression vector (pBARGPE1) carrying a phosphinothricin resistance gene (bar) as a selectable marker to yield the expression vector, pBARTvMnP2. Transformants were generated through genetic transformation using pBARTvMnP2. The genomic integration of the MnP clone was confirmed by PCR with bar-specific primers. One transformant showed higher enzyme activity than the recipient strain did, and was genetically stable even after 10 consecutive transfers on non-selective medium.
Clinical Features and Hearing Outcomes of Sudden Sensorineural Hearing Loss in Diabetic Patients
Ju Yeo Rim,Park Hyoung-sik,Lee Min Young,Jung Jae Yun,Choi Ji Eun 대한청각학회 2021 Journal of Audiology & Otology Vol.25 No.1
Background and Objectives: This study aimed to evaluate the clinical features and the clinical factors associated with prognosis of sudden sensorineural hearing loss (SSNHL) in diabetic patients.Subjects and Methods: Forty-nine diabetic with unilateral SSNHL were retrospectively included. All patients received systemic high dose steroid therapy within one month after onset and had more than one month of follow-up audiogram. The basic characteristics of the patients, initial and follow-up audiograms, laboratory data, and methods of steroid treatment were collected.Results: Compared to reference values in healthy subjects, 79%, 55%, and 45% of the patients had higher values of mean neutrophil-lymphocyte ratio (NLR), platelet-lymphocyte ratio (PLR), and lymphocyte-monocyte ratio (LMR), respectively. Older patients had significantly less degree of hearing loss, but they also had significantly worse hearing thresholds in the unaffected ear. After steroid treatment, less than half patients (47%) showed hearing recovery. Simultaneous intratympanic dexamethasone (ITD) injections with systemic steroid did not confer an additional hearing gain or an earlier recovery rate in diabetic patients with SSNHL. In the multivariate analysis, initial hearing thresholds of affected ear and timing of steroid treatment were significantly associated with hearing prognosis in diabetic patients with SSNHL.Conclusions: Diabetic patients with SSNHL tended to have increased NLR, LMR, and PLR, which are reported to be associated with microvascular angiopathy. Simultaneous ITD injections to improve hearing recovery in diabetic patients with SSNHL seems unnecessary.
Association of ZDHHC8 polymorphisms with smooth pursuit eye movement abnormality
Shin, Hyoung Doo,Park, Byung Lae,Bae, Joon Seol,Park, Tae Joon,Chun, Ji Yong,Park, Chul Soo,Sohn, Jin-Wook,Kim, Bong-Jo,Kang, Yeo-Hwa,Kim, Jae Won,Kim, Ki-Hoon,Shin, Tae-Min,Woo, Sung-Il Wiley Subscription Services, Inc., A Wiley Company 2010 American Journal of Medical Genetics. Part B Vol.b153 No.6
<P>The zinc finger DHHC domain-containing protein 8 (ZDHHC8) is located in the 22q11 microdeletion region and may contribute to the behavioral deficit associated with 22q11 deletion syndrome. Although polymorphisms of ZDHHC8 have been reported to be associated with the risk of schizophrenia, those associations are still controversial. This study was performed to validate the genetic association of ZDHHC8 polymorphisms with the risk of schizophrenia, and also to scrutinize the association with smooth pursuit eye movement (SPEM) abnormality in a Korean population. Five SNPs of ZDHHC8 were genotyped by TaqMan assay. Their genetic effects on the risk of schizophrenia were analyzed in 354 patients and 396 controls using allele-based χ<SUP>2</SUP> analyses. Association of ZDHHC8 polymorphisms with SPEM abnormality among 166 schizophrenic patients were analyzed using multiple regressions. No ZDHHC8 polymorphisms were found to be associated with the risk of schizophrenia. However, four SNPs and one haplotype (ht4) were strongly associated with the risk of SPEM abnormality even after multiple correction (P = 0.00005–0.0007, P<SUP>corr</SUP> = 0.0001–0.002). The results of the present study provide the first evidence that ZDHHC8 on the 22q11 locus might have influence on SPEM function of schizophrenia patients in a Korean population and may provide a new clue for understanding differential effects of candidate genes in schizophrenia. © 2010 Wiley-Liss, Inc.</P>
Jung, Min-Ho,Yoo, Jung-Min,Kang, Yeo-Jin,Lee, Hyoung Woo,Kim, Seung Hyun,Sung, Sang Hyun,Lee, Yong-Jin,Choi, Inho,Kim, Tack-Joong Pharmaceutical Society of Japan 2010 Biological & pharmaceutical bulletin Vol.33 No.6
<P>Muscle disorders, such as muscular dystrophy, are associated with an increase in oxidative stress. Proposed treatments for muscular dystrophy, some in clinical trials, include gene therapy and muscle cell transplantation. In this study, we investigated the effects of idesolide, isolated from the fruits of <I>Idesia polycarpa</I>, on changes that occur in muscle disuse atrophy. We noted protective effects on oxidative stress response and HSP70 regulation. Pre-treatment with idesolide for 24 h maintained cell viability and decreased apoptosis in H<SUB>2</SUB>O<SUB>2</SUB>-treated C<SUB>2</SUB>C<SUB>12</SUB> muscle cells. The idesolide pretreatment also increased intracellular HSP70 protein. Our results suggest that idesolide inhibits cell death through induction of HSP70 in C<SUB>2</SUB>C<SUB>12</SUB> muscle cells. This work is the first to report that idesolide can regulate the decrease in HSP70 that occurs during skeletal muscle atrophy.</P>
박보형 ( Bo Hyoung Park ),민정요 ( Jeong Yo Min ),여창동 ( Chang Dong Yeo ),오수성 ( Soo Seong Oh ),허기훈 ( Ki Hoon Hur ),허성은 ( Sung Eun Hur ),김진수 ( Jin Soo Kim ),김원철 ( Won Chul Kim ),김완욱 ( Wan Uk Kim ) 대한류마티스학회 2005 대한류마티스학회지 Vol.12 No.3
Adult onset Still`s disease (AOSD) is a multi-systemic inflammatory disorder characterized by several distinguished manifestations including high spiking fever, evanescent salmon-colored skin rash, arthralgia/arthritis, hepato-splenomegaly, lymphadenopathy, sore throat, serositis, and leukocytosis. The frequently noticed cardiopulmonary manifestation is pleuritis, pneumonitis, and pericarditis. Diffuse myocardial dysfunction is uncommon in AOSD, but it may be the cause of life-threatening heart failure. We have experienced a case of AOSD with acute heart failure in 20-year-old female complained of high fever and skin rash. On echocardiogram, the wall motion of left ventricle was globally decreased with a marked diminished ejection fraction (<25%). Two weeks after treatment with high dose steroid and intravenous immunoglobulin, her symptoms and cardiac function on echocardiogram was completely resolved. To our knowledge, this is the first case of AOSD with acute heart failure reported in Korea.