Effect of Hot-compaction Temperature on the Magnetic Properties of Anisotropic Nanocrystalline Magnets

Huijie Wang,Bin Lai,Min Lin,Dong Lee,Minggang Zhu,Wei Li 한국자기학회 2010 한국자기학회 학술연구발표회 논문개요집 Vol.2010 No.12

Shorter Telomere Length Is Associated with Increased Breast Cancer Risk in a Chinese Han Population: A Case-Control Analysis

Zhaoxia Wang,Zhenxing Zhang,Yanling Guo,Huifeng Shui,Guoqi Liu,Tianbo Jin,Huijie Wang 한국유방암학회 2018 Journal of breast cancer Vol.21 No.4

Purpose: The aim of this study was to investigate the association of telomere length with breast cancer risk. We simultaneously explored the association between telomerase reverse transcriptase gene polymorphisms and telomere length. Methods: We used real-time quantitative polymerase chain reaction to measure relative telomere length (RTL) in genomic DNA extracted from peripheral blood from 183 breast cancer cases and 191 healthy controls. Genotyping was performed using the Sequenom MassARRAY platform. Results: Our results show that breast cancer patients had significantly shorter RTLs than control subjects (p< 0.05). When the RTLs were categorized into tertiles, we found that the lowest RTL was significantly associated with increased breast cancer risk compared with the highest RTL (odds ratio [OR], 2.33; 95% confidence interval [CI], 1.40–3.90; p=0.001). Subgroup analyses indicated that risk of breast cancer was also significantly increased in the lowest RTL compared with the highest RTL in age >40 years (OR, 2.41; 95% CI, 1.31–4.43; p=0.005), body mass index ≤24 kg/m2 (OR, 2.81; 95% CI, 1.55–5.10; p=0.001), and postmenopausal women (OR, 3.94; 95% CI, 1.63–9.51; p=0.002), respectively. In addition, individuals with the AA genotype of rs2853677 have longer telomeres than those of breast cancer patients with the AG genotype (p= 0.011). Conclusion: Our results suggest that shorter RTL was associated with an increased risk of breast cancer. An association was found between the AA genotype of rs2853677 and longer RTLs in the case group. Functional studies are warranted to validate this association and further investigate our findings.

The upregulation of keratocan promotes the progression of human pancreatic cancer

Gao Huijie,Qian Ruikun,Ren Qiang,Zhang Litao,Qin Wei,Zhou Caiju,Wang Huiyun,Liu Chao,Zhang Yuntao 대한독성 유전단백체 학회 2024 Molecular & cellular toxicology Vol.20 No.2

Background The role of the extracellular matrix (ECM) in oncogenic contexts has been studied previously, but the expression patterns and functional role of keratocan, a classical small leucine-rich proteoglycan found in the ECM, in tumors remain poorly understood. As pancreatic cancer (PC) is characterized by desmoplasia in the ECM, this study sought to assess the significance of keratocan in PC. Objective In this study, Gene Expression Profiling Interactive Analysis (GEPIA) was first used to analyze the expression pattern of keratocan in PC. Keratocan, P53, and P21 levels were evaluated in PDAC patient tissues and the role of keratocan was additionally directly assessed via transfecting PDAC cell lines with a pENTER-human keratocan construct. Results Patients with PC showing high levels of keratocan had low survival rates. A significantly upregulated expression of keratocan was observed in the PC tumor tissues in comparison to healthy paracancerous tissues. Keratocan upregulation in BxPC-3 and PANC-1 cells markedly enhanced their proliferation and migration, but reduced cellular apoptosis. Furthermore, the P53 and P21 expression levels were significantly reduced in pancreatic ductal adenocarcinoma cells overexpressing keratocan. P53 and P21 were downregulated in PC tumor tissues. Conclusions All results showed that keratocan played important roles in the promoting of PC. Background The role of the extracellular matrix (ECM) in oncogenic contexts has been studied previously, but the expression patterns and functional role of keratocan, a classical small leucine-rich proteoglycan found in the ECM, in tumors remain poorly understood. As pancreatic cancer (PC) is characterized by desmoplasia in the ECM, this study sought to assess the significance of keratocan in PC. Objective In this study, Gene Expression Profiling Interactive Analysis (GEPIA) was first used to analyze the expression pattern of keratocan in PC. Keratocan, P53, and P21 levels were evaluated in PDAC patient tissues and the role of keratocan was additionally directly assessed via transfecting PDAC cell lines with a pENTER-human keratocan construct. Results Patients with PC showing high levels of keratocan had low survival rates. A significantly upregulated expression of keratocan was observed in the PC tumor tissues in comparison to healthy paracancerous tissues. Keratocan upregulation in BxPC-3 and PANC-1 cells markedly enhanced their proliferation and migration, but reduced cellular apoptosis. Furthermore, the P53 and P21 expression levels were significantly reduced in pancreatic ductal adenocarcinoma cells overexpressing keratocan. P53 and P21 were downregulated in PC tumor tissues. Conclusions All results showed that keratocan played important roles in the promoting of PC.

Simulation of the Die-upsetting Process of Hot-deformed Magnets

Bin Lai,Huijie Wang,Minggang Zhu,Wei Li 한국물리학회 2013 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.63 No.3

A plastic deformation simulation was performed using a three-dimensional finite-element methodbased deformation modeling software in order to clearly understand the die-upsetting process indetail. The simulation indicates that effective strains are different for different regions in thedie-upsetting process because of a limitation of the dies. Effective strains of Nd-Fe-B die-upsetmagnets have a parabolic reduction from the middles to the ends of the magnetic specimens alongthe c-axis. Experimentally, anisotropic Nd-Fe-B magnets were prepared from commercial melt-spunpowders (MQU-F) by hot pressing and subsequent die upsetting. The magnetic properties, the Xraydiffraction patterns and the microstructures of die-upset magnet specimens of different heightswere studied. The magnetic properties increased with the effective strains of the die-upset magnets. Experimental research confirmed that the microstructural characterics of die-upset magnets is dueto the inhomogeneity of the die-upsetting process.

Glia maturation factor beta deficiency protects against diabetic osteoporosis by suppressing osteoclast hyperactivity

Shi Si,Gu Huijie,Xu Jinyuan,Sun Wan,Liu Caiyin,Zhu Tong,Wang Juan,Gao Furong,Zhang Jieping,Ou Qingjian,Jin Caixia,Xu Jingying,Chen Hao,Li Jiao,Xu Guotong,Tian Haibin,Lu Lixia 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Excessive osteoclast activation, which depends on dramatic changes in actin dynamics, causes osteoporosis (OP). The molecular mechanism of osteoclast activation in OP related to type 1 diabetes (T1D) remains unclear. Glia maturation factor beta (GMFB) is considered a growth and differentiation factor for both glia and neurons. Here, we demonstrated that Gmfb deficiency effectively ameliorated the phenotype of T1D-OP in rats by inhibiting osteoclast hyperactivity. In vitro assays showed that GMFB participated in osteoclast activation rather than proliferation. Gmfb deficiency did not affect osteoclast sealing zone (SZ) formation but effectively decreased the SZ area by decreasing actin depolymerization. When GMFB was overexpressed in Gmfb-deficient osteoclasts, the size of the SZ area was enlarged in a dose-dependent manner. Moreover, decreased actin depolymerization led to a decrease in nuclear G-actin, which activated MKL1/SRF-dependent gene transcription. We found that pro-osteoclastogenic factors (Mmp9 and Mmp14) were downregulated, while anti-osteoclastogenic factors (Cftr and Fhl2) were upregulated in Gmfb KO osteoclasts. A GMFB inhibitor, DS-30, targeting the binding site of GMFB and Arp2/3, was obtained. Biocore analysis revealed a high affinity between DS-30 and GMFB in a dose-dependent manner. As expected, DS-30 strongly suppressed osteoclast hyperactivity in vivo and in vitro. In conclusion, our work identified a new therapeutic strategy for T1D-OP treatment. The discovery of GMFB inhibitors will contribute to translational research on T1D-OP.

Physiological and omics analysis of maize inbred lines during late grain development

Jin Xining,Zhai Huijie,Wang Pingxi,Zhang Xiaoxiang,Wu Xiangyuan,Zhang Huaisheng,Chen Shilin,Huang Zhongwen 한국유전학회 2022 Genes & Genomics Vol.44 No.8

Background: There were significant differences in the change of moisture content and grain composition at the late stage of grain development among different maize varieties, but the regulation mechanism is not clear. Objective: To explore the key genes causing the variation in physiological traits of two typical maize inbred lines in late grain development. Methods: The grains at different development stages were selected as materials to determine the content of water, sucrose, starch and ABA. Transcriptomic and proteomic analysis of the materials were performed to screen relevant genes. Results: The grain dehydration rate and the content of sucrose, starch and ABA were showed significant differences between two varieties in the late stage of grain development. The enrichment analysis of common differentially expressed genes (proteins) showed that most of the genes (proteins) were enriched in the extracellular region. The downregulated genes were mainly concentrated in carbohydrate metabolism and lipid metabolism, while the upregulated genes were mainly in response to stress. Furthermore, this study also identified many key candidate genes (dehydrin genes, pathogenesis-related genes, sucrose synthase and secondary metabolites related genes) related to late grain development of maize. Conclusions: The suggested genes related to late grain development of maize can be candidates for further functional study.

The influence of wheel/rail gap and rotating mechanism on dynamic performance of APM

Yuanjin Ji,Lihui Ren,Huijie Wang 대한기계학회 2017 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.31 No.11

The dynamics equations of Automatic passenger mover (APM) vehicle with the middle-guiding single-axle tire running gears were derived using linearized tire model, in which side force and rolling contact effect on running tire are taken account. A simulation model of APM vehicle based on Simulink environment was built using object-oriented programming technique, and the effects of contact statues between guiding tires and rail and different rotation modes on of curving performance and ride quality were studied. The simulation has shown that the preload force on guiding tires don’t improve curving performance but significantly worsen lateral ride quality, whereas the gap between guiding tires and rail will increase the running tires side force and guiding tires radial force on curve. The gap between guide tires and rail should be controlled between 0 and 5 mm on compromising the curving performance and ride quality of APM vehicle. The cornering force of the rotating mechanism above the springs mode is greater than that of the below mode, and decreases with the increase of the curve radius, which is unfavorable to the curve passing.

A Study on Fracture Behavior and Impact Stability of Sintered Rare-earth Permanent Magnets

Li Wei,Li Anhua,Wang Huijie,Dong Shengzhi,Guo Yongquan 한국분말야금학회 2006 한국분말야금학회 학술대회논문집 Vol.2006 No.1

The fracture behavior and mechanical characteristics of sintered rare-earth magnets were investigated. It shows that the fracture behavior and bending strength of the magnets obviously exhibit anisotropy. Sm-Co magnets tend to cleavage fracture in the close-packed (0001) plane or in the (10 11 ) plane. The fracture mechanism of Nd2Fe14B magnet mainly appears to be intergranular fracture. The anisotropy of fracture behavior and mechanical strength of sintered rare-earth magnets is caused mainly by the strong crystal-structure anisotropy and the grain alignment texture. The effects of Nd content, and Pr, Dy substitution on the impact stability of Nd2Fe14B magnets were also reported.

Multilevel resistive switching and synaptic behaviors in MnO-based memristor

Ai Ruibo,Zhang Tao,Guo Huijie,Luo Wang,Liu Xiaojun 한국물리학회 2022 Current Applied Physics Vol.41 No.-

Exploring new synaptic electronic devices that combine computing and memory is a promising strategy that fundamentally approaches intelligent machines. In this study, the multilevel resistive switching and synaptic behaviors of a MnO-based device is studied. The device is composed of Al/MnO/Ni sandwich structure, has stable resistance switching characteristics, has continuous nonvolatile memory state, can be used as electrically programmable and erasable analog memory. The gradual conductance modulation is realized by changing the compliance current and the maximum scanning voltage. The Al/MnO/Ni devices successfully mimic the basic functions of synapses, including the paired-pulse facilitation, spike-rate-dependent plasticity, excitatory postsynaptic current, short-term plasticity, long-term plasticity, and sike-timing-dependent plasticity.

Preparation of epoxypropyl functionalized graphene oxide and its anticorrosion properties complexed with epoxy resin

Xiaofeng Yang,Yuepeng Zhang,Zhiping Chen,Yinshuai Yang,Hongxia Jing,Zhenhao Sun,Huijie Wang 한국화학공학회 2020 Korean Journal of Chemical Engineering Vol.37 No.12

Functionalized graphene oxide (GO)/epoxy resin (EP) composite coating has attracted attention due to its advantages of epoxy resin in acid resistance, anticorrosion and having the characteristics of GO such as good chemical stability and strong electrolyte barrier properties. In this study, epoxypropyl functionalized graphene oxide (EFGO) was synthesized with a new strategy by grafting epichlorohydrin (ECH) onto the surface of GO. EFGO was then crosslinked with EP by diethylenetriamine (DETA) to fabricate EFGO/EP anticorrosive coating. The structure and property of EFGO and EFGO/EP were characterized and evaluated by various methods such as FTIR, Raman, XPS, SEM, and EIS. The results showed that the graft amount of epoxypropyl on GO surface could be effectively increased by using KI-KF catalysis system. The increase of the graft amount further improved the compatibility between EFGO and EP. EFGO/EP had a network structure with EFGO as the “central node”. The “central node” locked the movement of EP chain segment, as improving the thermal stability of EFGO/EP. The EFGO/EP coating fabricated by the EFGO, which grafted larger epoxypropyl, had good anticorrosion performance and adhesion with steel plates, showing a good barrier effect on electrolyte migration. The coating resistance reached up 4.88×108 cm2.