Dietary <i>Bacillus subtilis</i> B2A strain in laying hens challenged with <i>Salmonella gallinarum</i>: effects on egg production, egg quality, blood haptoglobin and targeted intestinal <i>Salmonella</i> shedding

Hosseindoust, Abdolreza,Mohammadi, Mohsen,Yao, Zhao Pin,Jung, Migyeong,Kim, In Ho Informa UK (TaylorFrancis) 2018 Journal of applied animal research Vol.46 No.1

Effects of Bacillus subtilis, Kefir and β-Glucan Supplementation on Growth Performance, Blood Characteristics, Meat Quality and Intestine Microbiota in Broilers

Hosseindoust, Abdolreza,Park, Jae Won,Kim, In Ho The Korean Society of Poultry Science 2016 韓國家禽學會誌 Vol.43 No.3

A total of 528 broilers ($47{\pm}0.1g$; 1 day of age) were used in a 35-day feeding trial to evaluate probiotics, prebiotics and their interactive effects on growth performance, blood characteristics, relative organ weight and meat quality. Broilers were randomly distributed into 1 of 6 treatments on the basis of body weight (BW) (4 replicate pens per treatment, 22 broilers per pen). The dietary treatments were CON, basal diet; B, CON + 0.1 g kg-1 Bacillus subtilis; K, CON + 1 g kg-1 Kefir; G, CON + 1 g kg-1 ${\beta}-glucan$; GB, G + 1 g kg-1 Bacillus subtilis; and GK, G + 1 g kg-1 Kefir. The overall effects indicated that broilers fed the K, G and GK diets had greater body weight gain (BWG) than those fed the CON diet (P<0.05). The number of white blood cells increased (P<0.05) in the GB group compared with the CON, B and G treatments; however, the lymphocyte percentage in the B group was higher than in the G group. The weight of bursa fabricii was lower in the B and G groups compared to the K group (P<0.05), whereas a higher spleen weight was observed in chickens that were fed the GB and GK diets compared to the B group (P<0.05). The treatments did not affect the meat quality parameters, except for meat redness, which improved with all of the supplementation groups (P<0.05). The population of Lactobacillus spp. in gizzard was significantly higher in the K treatment compared with CON, B, G and GB. In conclusion, supplementation with kefir and ${\beta}-glucans$ improved growth performance.

Effects of meal processing of black soldier fly on standardized amino acids digestibility in pigs

Abdolreza Hosseindoust,Sang Hun Ha,Jun Young Mun,Jin-Soo Kim 한국축산학회 2023 한국축산학회지 Vol.65 No.5

The aim of this study was to investigate the effect of incorporating black soldier fly (BSF) larvae and its processed form as an alternative source of protein to fish meal (FM) on the digestibility of amino acids (AA) in weaned pigs. Four cannulated pigs with an initial bodyweight of 13.25 ± 0.25 kg and aged 30 days were subjected to a 4 × 4 Latin square design with three treatments, as well as a nitrogen-free treatment. The diets used for each treatment consisted of a FM diet, a diet containing BSF larvae meal (BSFM), and a diet containing extruded BSF (BSFE). The study was conducted over four stages, with a total duration of 28 days. The apparent ileal digestibility (AID) of protein was higher in the FM treatment compared with the BSFM. Among essential AA, the AID of Arg, His, Leu, and Thr were higher in the FM compared with the BSFM and BSFE. A greater AID of Ile and Phe was observed in pigs in the FM treatment compared with the BSFM. The average AA digestibility did not show any difference between treatments. Among non-essential AA, the AID of Ala (p = 0.054) and Glu (p = 0.064) tended to be increased in the FM compared with the BSFM. Among essential AA, the standardized ileal digestibility (SID) of Arg, His, Ile, and Leu were higher in the FM compared with the BSFM. Among non-essential AA, the SID of Cys (p = 0.074) tended to be increased in the FM compared with the BSFM. In conclusion, the processing and thermal conditioning techniques utilized for BSF larvae meal showed a tendency for increased AA digestibility. Therefore, when formulating a diet, it is important to take into account the difference in AA digestibility between FM and BSFM.

Effects of Bacillus subtilis, Kefir and β-Glucan Supplementation on Growth Performance, Blood Characteristics, Meat Quality and Intestine Microbiota in Broilers

Abdolreza Hosseindoust,Jae Won Park,In Ho Kim 한국가금학회 2016 韓國家禽學會誌 Vol.43 No.3

A total of 528 broilers (47±0.1 g; 1 day of age) were used in a 35-day feeding trial to evaluate probiotics, prebiotics and their interactive effects on growth performance, blood characteristics, relative organ weight and meat quality. Broilers were randomly distributed into 1 of 6 treatments on the basis of body weight (BW) (4 replicate pens per treatment, 22 broilers per pen). The dietary treatments were CON, basal diet; B, CON + 0.1 g kg-1 Bacillus subtilis; K, CON + 1 g kg-1 Kefir; G, CON + 1 g kg-1 β-glucan; GB, G + 1 g kg-1 Bacillus subtilis; and GK, G + 1 g kg-1 Kefir. The overall effects indicated that broilers fed the K, G and GK diets had greater body weight gain (BWG) than those fed the CON diet (P<0.05). The number of white blood cells increased (P<0.05) in the GB group compared with the CON, B and G treatments; however, the lymphocyte percentage in the B group was higher than in the G group. The weight of bursa fabricii was lower in the B and G groups compared to the K group (P<0.05), whereas a higher spleen weight was observed in chickens that were fed the GB and GK diets compared to the B group (P<0.05). The treatments did not affect the meat quality parameters, except for meat redness, which improved with all of the supplementation groups (P<0.05). The population of Lactobacillus spp. in gizzard was significantly higher in the K treatment compared with CON, B, G and GB. In conclusion, supplementation with kefir and β-glucans improved growth performance.

환경 온도와 등지방두께가 포유모돈의 번식성적, 혈액 특성, 호르몬 성상 및 유 성상에 미치는 영향

이석희,김광열,Hosseindoust Abdolreza,최요한,김태균,문준영,Joseph Moturi,김진수,채병조 강원대학교 동물생명과학연구소(구 강원대학교 동물자원공동연구소) 2020 동물자원연구 Vol.31 No.4

본 연구는 환경 온도와 등지방 두께(BT)가 모돈의 번식능력에 미치는 영향을 조사하기 위해서 수행되었다. 실험은 포유모돈 60두를 공시하였고, 2×2 factorial arrangement에 따라 각 처리구는 두 수준의 사육 온도(high temperature [HT], 27.5±1.76℃; low temperature [LT], 23.3±0.89℃) 및 BT(<20mm, 평균 17.70mm; ≥20mm, 평균 23.20mm)로 나누어 2019년 7월부터 8월까지 2개월 동안 실시하였다. 실험 결과 HT 처리구에서 등지방두께 변이폭이 크고, 사료 섭취량은 적은 것으로 나타났다. <20mm BT 처리구가 ≥20mm BT 처리구에 비하여 체중 손실과 등지방 손실이 적은 것으로 나타났다. <20mm BT 처리구의 재귀발정일(<20mm 5.20, 4.93d vs. ≥20mm 5.87, 5.60d)이 ≥20mm BT 처리구보다 짧은 것으로 나타났다. 포유자돈 생존률은 LT 처리구(94.87%) 보다 HT 처리구(90.31%)에서 더 낮은 것으로 나타났다. 자돈의 이유체중과 일일증체량은 BT가 ≥20mm 이상인 모돈(각각 5.49kg 및 188.41g)보다 BT가 <20mm미만인 모돈(각각 5.75kg 및 201.46g)에서 더 큰 것으로 나타났다. HT 처리구의 모돈은 LT 처리구의 모돈 보다 코티솔 농도가 더 높았다(post farrowing: HT 7.86ug/mL vs. LT 6.04ug/mL; weanling: HT 5.48ug/mL vs. LT 4.40ug/mL). 결론적으로 환경 온도는 모돈의 번식과 코티솔 수치에 부정적인 영향을 미치는 것으로 나타났다. 또한, BT가 높은 모돈은 열 스트레스를 받을 때 이유에서 발정까지의 간격이 더 길었다. 본 실험 결과는 실제 농가에서 고온기에 모돈관리하는데 활용될 것으로 보여진다. The present study was conducted to investigate the effect of environmental temperature and backfat thickness (BT) on the reproductive performance of lactating sows. Sixty crossbred sows were allotted to four groups in a 2 × 2 factorial arrangement by environmental temperature (high temperature [HT], 27.5±1.76℃; low temperature [LT], 23.3±0.89℃) and BT (< 20 mm, average 17.70 mm; ≥ 20 mm, average 23.20 mm) from July to August 2019. Sows in the HT group experienced a greater change in BT and a lower feed intake. Losses in body weight and BT were lower in sows with < 20 mm BT than in those with ≥ 20 mm BT. Sows with low BT had a lower weaning-to-estrus interval than sows with high BT (5.20, 4.93 d vs. 5.87, 5.60 d, respectively). Piglet survivability was lower in the HT group (90.31%) than in the LT group (94.87%). Piglet weaning weight and average daily weight gain were greater in sows with < 20 mm BT (5.75 kg and 201.46 g, respectively) than in those with ≥ 20 mm BT (5.49 kg and 188.41 g, respectively). Sows in the HT group had higher cortisol concentrations than those in the LT group (post-farrowing: HT 7.86 µg/mL vs. LT 6.04 µg/mL; weanling: HT 5.48 µg/mL vs. LT 4.40 µg/mL). In conclusion, environmental temperature adversely influenced sow performance and cortisol levels. Moreover, sows with low BT had a greater weaning-to-estrus interval when subjected to heat stress.

Effect of Dry, Wet and Liquid Feeding on the Performance, Digestibility and Carcass Characteristics of Growing Pigs

김진수,Abdolreza Hosseindoust,이수협,최요한,노현석,채병조 강원대학교 동물자원공동연구소 2015 동물자원연구 Vol.26 No.2

A total of 240 growing pigs were distributed in three treatment groups to investigate the influence of fermentation in different feeder type on the growing finishing pigs. The treatments were dry feeding (DF), wet feeding (WF) with dry-wet feeders and liquid feeding (LF) with freshly prepared 3:1 water to feed ratio fed three times a day throughout the experiment. The average daily gain (ADG) and body weight were consistently greater (p<0.05) in LF than the others. When the entire experimental period was taken under consideration the ADG and body weight was also found to be increased (p<0.05) in WF in comparison to DF. The average daily feed intake (ADFI) and growth to feed ratio (G/F) was not affected however the average daily water intake (ADWI) and water to feed ratio (W/F) were significantly reduced (p<0.01) in WF in comparison to DF and LF. The ATTD of DM, GE and CP was increased (p<0.05) in WF and LF in comparison to DF at both phase I and II (4th and 8th wk) of the experiment. Carcass characteristics and blood parameters were not affected (p>0.05) in any of the feeding type in growing finishing pigs. It can be concluded that wet feeding with dry-wet feeders is good for enhancing the growth performance in the later stages while fresh liquid feeding in ratio 3:1 is beneficial for the growing finishing pigs throughout the experiment.

Effects of Ecklonia cava as fucoidan-rich algae on growth performance, nutrient digestibility, intestinal morphology and caecal microflora in weanling pigs

Choi, Yohan,Hosseindoust, Abdolreza,Goel, Akshat,Lee, Suhyup,Jha, Pawan Kumar,Kwon, Ill Kyong,Chae, Byung-Jo Asian Australasian Association of Animal Productio 2017 Animal Bioscience Vol.30 No.1

Objective: In the present study, role of increasing levels of Ecklonia cava (seaweed) supplementation in diets was investigated on growth performance, coefficient of total tract apparent digestibility (CTTAD) of nutrients, serum immunoglobulins, cecal microflora and intestinal morphology of weanling pigs. Methods: A total of 200 weaned pigs (Landrace${\times}$Yorkshire${\times}$Duroc; initial body weight $7.08{\pm}0.15kg$) were randomly allotted to 4 treatments on the basis of body weight. There were 5 replicate pens in each treatment including 10 pigs of each. Treatments were divided by dietary Ecklonia cava supplementation levels (0%, 0.05%, 0.1%, or 0.15%) in growing-finishing diets. There were 2 diet formulation phases throughout the experiment. The pigs were offered the diets ad libitum for the entire period of experiment in meal form. Results: The pigs fed with increasing dietary concentrations of Ecklonia cava had linear increase (p<0.05) in the overall average daily gain, however, there were no significant differences in gain to feed ratio, CTTAD of dry matter and crude protein at both phase I and phase II. Digestibility of gross energy was linearly improved (p<0.05) in phase II. At day 28, pigs fed Ecklonia cava had greater (linear, p<0.05) Lactobacillus spp., fewer Escherichia coli (E. coli) spp. (linear, p<0.05) and a tendency to have fewer cecal Clostridium spp. (p = 0.077). The total anaerobic bacteria were not affected with supplementation of Ecklonia cava in diets. Polynomial contrasts analysis revealed that villus height of the ileum exhibited a linear increase (p<0.05) in response with the increase in the level of dietary Ecklonia cava. However, villus height of duodenum and jejunum, crypt depth, villus height to crypt depth ratio of different segments of the intestine were not affected. Conclusion: The results suggest that Ecklonia cava had beneficial effects on the growth performance, cecal microflora, and intestinal morphology of weanling pigs.

Evaluation of high nutrient diets on litter performance of heat-stressed lactating sows

Choi, Yohan,Hosseindoust, Abdolreza,Shim, YoungHo,Kim, Minju,Kumar, Alip,Oh, Seungmin,Kim, YoungHwa,Chae, Byung-Jo Asian Australasian Association of Animal Productio 2017 Animal Bioscience Vol.30 No.11

Objective: The present study investigated the litter performance of multiparous sows fed 3% and 6% densified diets at farrowing to weaning during summer with mean maximum room temperature of $30.5^{\circ}C$. Methods: A total of 60 crossbred multiparous sows were allotted to one of three treatments based on body weight according to a completely randomized design. Three different nutrient levels based on NRC were applied as standard diet (ST; metabolizable energy, 3,300 kcal/kg), high nutrient level 1 (HE1; ST+3% higher energy and 16.59% protein) and high nutrient level 2 (HE2; ST+6% higher energy and 17.04% protein). Results: There was no variation in the body weight change. However, backfat thickness change tended to reduce in HE1 in comparison to ST treatment. Dietary treatments had no effects on feed intake, daily energy intake and weaning-to-estrus interval in lactating sows. Litter size, litter weight at weaning and average daily gain of piglets were significantly greater in sows in HE1 compared with ST, however, no difference was observed between HE2 and ST. Increasing the nutrient levels had no effects on the blood urea nitrogen, glucose, triglyceride, and creatinine at post-farrowing and weaning time. The concentration of follicle stimulating hormone, cortisol and insulin were not affected by dietary treatments either in post-farrowing or weaning time. The concentration of blood luteinizing hormone of sows in ST treatment was numerically less than sows in HE2 treatment at weaning. Milk and colostrum compositions such as protein, fat and lactose were not affected by the treatments. Conclusion: An energy level of 3,400 kcal/kg (14.23 MJ/kg) with 166 g/kg crude protein is suggested as the optimal level of dietary nutrients for heat stressed lactating sows with significant beneficial effects on litter size.

건식, 습식 및 건습식 사료급이가 육성돈의 성장, 소화율 및 도체 특성에 미치는 영향

김진수,Abdolreza Hosseindoust,이수협,최요한,노현석,채병조 강원대학교 동물생명과학연구소(구 강원대학교 동물자원공동연구소) 2015 동물자원연구 Vol.26 No.2

A total of 240 growing pigs were distributed in three treatment groups to investigate the influence of fermentation in different feeder type on the growing finishing pigs. The treatments were dry feeding (DF), wet feeding (WF) with dry-wet feeders and liquid feeding (LF) with freshly prepared 3:1 water to feed ratio fed three times a day throughout the experiment. The average daily gain (ADG) and body weight were consistently greater (p<0.05) in LF than the others. When the entire experimental period was taken under consideration the ADG and body weight was also found to be increased (p<0.05) in WF in comparison to DF. The average daily feed intake (ADFI) and growth to feed ratio (G/F) was not affected however the average daily water intake (ADWI) and water to feed ratio (W/F) were significantly reduced (p<0.01) in WF in comparison to DF and LF. The ATTD of DM, GE and CP was increased (p<0.05) in WF and LF in comparison to DF at both phase I and II (4th and 8th wk) of the experiment. Carcass characteristics and blood parameters were not affected (p>0.05) in any of the feeding type in growing finishing pigs. It can be concluded that wet feeding with dry-wet feeders is good for enhancing the growth performance in the later stages while fresh liquid feeding in ratio 3:1 is beneficial for the growing finishing pigs throughout the experiment.

에너지 수준이 다른 사료에 복합효소제의 첨가가 육계의 사양성적, 영양소 소화율, 혈액성상, 장내미생물 균총 및 소장 융모에 미치는 영향

심영호,김진수,Hosseindoust Abdolreza,Santosh Laxman Ingale,최요한,김민주,오승민,함형빈,채병조 강원대학교 동물생명과학연구소(구 강원대학교 동물자원공동연구소) 2017 동물자원연구 Vol.28 No.3

The present study was conducted to investigate the effects on growth performance, nutrient digestibility, and gut health of broiler chickens when a dietary supplementation of multienzymes was added to diets, containing different energy levels. A total of 480 broiler chickens of similar body weight (Ross 308, 1-day-old) were randomly subjected to four treatments. The dietary treatments included a corn-soybean meal-based diet supplemented with: multienzyme (amylase+protease+ mannanase+xylanase+phytase), 0.05% enzyme, and different energy levels (3010 and 3060 kcal/kg). The experimental diets were fed to the chicks in a mash form for 35 days in two phases (1–21 d, phase I; and 22–35 d, phase II). During the overall period, chicks fed with diets supplemented with multienzymes had a better weight gain (p<0.05) and feed conversion ratio (FCR) than those fed with diets without enzymes. There was no difference in the growth rate and FCR among the chicks fed with diets supplemented with enzymes, even though the dietary energy levels were different. The apparent fecal and ileal digestibility of dry matter, gross, crude protein, calcium, and phosphorus were significantly enhanced (p<0.05). The population of cecal and ileal Lactobacillus spp. was significantly increased (p<0.05), and Clostridium spp. and coliforms were significantly decreased (p<0.05) in diets supplemented with enzymes. Villus height and villus height to crypt depth ratio in the small intestine was also significantly enhanced (p<0.05) in diets supplemented with enzymes. In conclusion, multienzyme supplementation had positive effects on the weight gain of broilers, FCR, digestibility of nutrients, and on the growth of intestinal microbiota.