Effect of Developmental Lead Exposure on the Expression of Hippocampal NMDA Receptor Subunit mRNA

Kim, Tae-Wan,Chung, In-Sung,Bae, Jae-Hoon,Shin, Dong-Hoon,Lee, Mi-Young,Kim, Joon-Sik 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

목적: in vivo 및 vitro에서 해마 신경세포의 발생단계별 NMDA 수용체 아단위 mRNA 발현에 대한 연 폭로 영향을 알아보고자 하였다. 방법: 흰쥐 해마 신경세포의 발생단계별 NMDA 수용체 NR2A, NR2B 아단위 mRNA 발현에 대한 연의 영향은 정상군과 연 폭로군의 출생 후 7일, 14일, 22일 흰쥐의 해마에서 in situ hybridization으로 mRNA 발현 정도를 densitometer로 측정하여 비교하였고, 연과 NMDA 의 세포독성은 해마 신경세포 일차배양 후 도립현미경을 이용한 형태학적인 관찰과 LDH 활성도를 이용하여 측정하였다. 결과: 연 과 NMDA 에 의한 세포독성에 대한 in vitro 실험에서 형태학적 소견과 LDH 활성도에서 해마 미성숙 신경세포와 성숙 신경세포사이의 차이가 있었으므로, 연과 NMDA 독성효과는 해마 신경세포의 발달 단계에 따라 차이가 있다. 정상군의 해마에서의 NR2A mRNA 발현은 출생 후 연령이 증가함에 따라 점진적으로 증가하였으나, NR2B mRNA 발현은 출생 후 연령이 증가함에 따라 점진적으로 증가하였으나 NR2B mRNA 발현은 연령의 증가에 따른 변화가 없었다. 연 폭로에 희한 NR2A mRNA 발현은 유의하게 감소하였으나(p<0.05), NR2B mRNA 발현은 변화가 나타나지 않았다. 만성적 연 폭로는 NR2A를 포함하는 NMDA 수용체를 감소시킬 수 있음을 알 수 있다. 결론: 연은 해마신경세포의 발생단계에서 NMDA 수용체 아단위 특히 NR2A mRNA 발현의 변화를 야기하여 시냅스 신호 전달에 영향을 나타냄을 알 수 있었다. Hippocampus

Antiadhesive effect and safety of oxidized regenerated cellulose after thyroidectomy

Kyoung Sik Park,Kyu Eun Lee,Do Hoon Ku,Su-Jin Kim,Won Seo Park2,Hoon Yub Kim3,,Mi Ra Kwon1,Yeo-Kyu Youn1 대한외과학회 2013 Annals of Surgical Treatment and Research(ASRT) Vol.84 No.6

Purpose: To evaluate the antiadhesive effects and safety of an oxidized regenerated cellulose (Interceed) after thyroidectomy. Methods: Seventy-six thyroidectomized patients were prospectively randomized into two groups with regard to the use of Interceed. We evaluated each group for their adhesive symptoms using four subjective and four objective items at the 2nd week, 3rd and 6th month after thyroidectomy. All patients were examined for vocal cord motility by indirect laryngoscope at each period. Results: Total adhesion scores at each postoperative follow-up period decreased with time, but were not significantly different in each group. The median score for swallowing discomfort for liquid was significantly lower in the Interceed group than in the control group 2 weeks after surgery. In addition, the severity of skin adhesion to the trachea was reduced in the Interceed group compared with the control group 6 months after surgery. During the study, there were no adverse effects or significant differences in postoperative complications between the groups. Conclusion: Interceed appeared to be safe and effective in improving neck discomfort at early postoperative periods and preventing skin adhesion to the trachea 6 months after thyroidectomy.

Protective effect of Korean Red Ginseng against FK506-induced damage in LLC-PK1 cells

Lee, Dahae,Kang, Ki Sung,Yu, Jae Sik,Woo, Jung-Yoon,Hwang, Gwi Seo,Eom, Dae-Woon,Baek, Seung-Hoon,Lee, Hye Lim,Kim, Ki Hyun,Yamabe, Noriko The Korean Society of Ginseng 2017 Journal of Ginseng Research Vol.41 No.3

Background: Compound FK506 is an immunosuppressant agent that is frequently used to prevent rejection of solid organs upon transplant. However, nephrotoxicity due to apoptosis and inflammatory response mediated by FK506 limit its usefulness. In this study, the protective effect of Korean Red Ginseng (KRG) against FK506-induced damage in LLC-PK1 pig kidney epithelial cells was investigated. Methods: LLC-PK1 cells were exposed to FK506 with KRG and cell viability was measured. Western blotting and RT-PCR analyses evaluated protein expression of MAPKs, caspase-3, and KIM-1. TLR-4 gene expression was assessed. Caspase-3 activities were also determined. The number of apoptotic cells was measured using an image-based cytometric assay. Results: The reduction in LLC-PK1 cell viability by $60{\mu}M$ FK506 was recovered by KRG cotreatment in a dose-dependent manner. The phosphorylation of p38, p44/42 MAPKs (ERK), KIM-1, cleaved caspase-3, and TLR-4 mRNA expression was increased markedly in LLC-PK1 cells treated with $60{\mu}M$ FK506. However, with the exception of p-ERK, elevated levels of p-p38, KIM-1, cleaved caspase-3, and TLR-4 mRNA expression were significantly decreased after cotreatment with KRG. Activity level of caspase-3 was also attenuated by KRG cotreatment. Moreover, image-based cytometric assay showed that apoptotic cell death was increased by $60{\mu}M$ FK506 treatment, whereas it was decreased after cotreatment with KRG. Conclusion: Taken together, these results suggest that the molecular mechanism of KRG in the FK506-induced nephrotoxicity may lead to the development of an adjuvant for the inhibition of adverse effect FK506 in the kidney.

Protective effect of Korean Red Ginseng against FK506-induced damage in LLC-PK1 cells

Dahae Lee,Ki Sung Kang,Jae Sik Yu,Jung-Yoon Woo,Gwi Seo Hwang,Dae-Woon Eom,Seung-Hoon Baek,Hye Lim Lee,Ki Hyun Kim,Noriko Yamabe 고려인삼학회 2017 Journal of Ginseng Research Vol.41 No.3

Background: Compound FK506 is an immunosuppressant agent that is frequently used to prevent rejection of solid organs upon transplant. However, nephrotoxicity due to apoptosis and inflammatory response mediated by FK506 limit its usefulness. In this study, the protective effect of Korean Red Ginseng (KRG) against FK506-induced damage in LLC-PK1 pig kidney epithelial cells was investigated. Methods: LLC-PK1 cells were exposed to FK506 with KRG and cell viability was measured. Western blotting and RT-PCR analyses evaluated protein expression of MAPKs, caspase-3, and KIM-1. TLR-4 gene expression was assessed. Caspase-3 activities were also determined. The number of apoptotic cells was measured using an image-based cytometric assay. Results: The reduction in LLC-PK1 cell viability by 60mM FK506 was recovered by KRG cotreatment in a dose-dependent manner. The phosphorylation of p38, p44/42 MAPKs (ERK), KIM-1, cleaved caspase-3, and TLR-4 mRNA expression was increased markedly in LLC-PK1 cells treated with 60mM FK506. However, with the exception of p-ERK, elevated levels of p-p38, KIM-1, cleaved caspase-3, and TLR-4 mRNA expression were significantly decreased after cotreatment with KRG. Activity level of caspase-3 was also attenuated by KRG cotreatment. Moreover, image-based cytometric assay showed that apoptotic cell death was increased by 60mM FK506 treatment, whereas it was decreased after cotreatment with KRG. Conclusion: Taken together, these results suggest that the molecular mechanism of KRG in the FK506-induced nephrotoxicity may lead to the development of an adjuvant for the inhibition of adverse effect FK506 in the kidney.

SOL-GEL法에 依한 LAS系 結晶化유리의 製造에 있어서 租成比유리의 特性에 미치는 影響

김봉걸,양중식,조훈성,권창오,박경호 慶熙大學校 材料科學技術硏究所 1990 材料科學技術硏究論集 Vol.3 No.-

The purpose of this study is to investigate into the physical properties of Li₂O-Al₂O₃-nSiO₂ (LAnS) system of crystallized glass prepared by sol-gel method according to the composition ratio of LAnS. In order to find the crystallization temperature as well as the state of crystalline, differential thermal analysis and X-ray diffraction analysis were examined. The results obtained are as follows : 1. Gelling time became longer as increasing the composition ratio of SiO₂ and also found that it became faster as increasing the additive amount of water. Also, additive amount of water required for the gellation was above four times of the theoretical amount of water required hydrolysis. 2. Surface area and mean pore size became larger as increasing the composition ratio of SiO₂ and for LA4S dried gel, surface area was 331.1m^(2)/g, mean pore size distributed about 5nm and 30-50nm. 3. The crystallization temperature is high as increasing the composition ratio of SiO₂ and for LA4S, crystallization temperature is 800℃. Also, LA4S formed at 800℃ for the crystalline phase of β-eucryptite and at 900℃ for crystalline phase of β-spodumene.

木連屬 植物의 花粉發芽에 관한 硏究

金薰植,李承雨 경희대학교 부설 식량자원개발연구소 1989 硏究論文集 Vol.10 No.-

Research was conducted to investigate the effects of media composition on pollen germination and pollen tube growth in Magnolia spp. Boron remarkably promoted the pollen germination and pollen tube growth. However, no pollen germination occurred on the media containing no boron in M. sieboldii and M. obovata. The highest capacity for pollen germination and pollen tube growth was obtained with the combination of 10% sucrose and l00ppm boron. On the other hand, K, Ca, and Mg did not promote the pollen germination and pollen tube growth in both species. For the pollen tube growth, however, M. denudata showed higher sensitivity than M. liliflora to medium acidity. In M. denudata, low GA_(3) concentration in the medium hastened the pollen germination, while pollen tube growth was retarded by high GA_(3) concentration. In contrast to GA_(3), no pollen germination occurred on the medium containing 50ppm and 100ppm IBA. The capacity for the pollen germination was markedly higher in M. liliflora and M. denudata than in M. sieboldii and M. obovata.

16세기 군신윤리의 변화와 출처론

김훈식 한국역사연구회 2003 역사와 현실 Vol.- No.50

One of the most typical and unanimously received ultimatum regarding the Confucian concept of the relationship between the King and the Subjects(including the vassals), is that they are bonded with justice and moral obligations. What the historians should keep in mind in analyzing this ultimatum, is that the contents of those justifications and moral obligations used to change to same extent, due to the trends and unique atmospheres of certain subject periods. In this article, the fact that the moral obligations regarding the matter of whether to serve the government or choose otherwise(like retiring) became a dominant subject, is closely examined. Along with the heightened sense of justice based on moral reasoning(名分論的義理), which in itself strongly supported undisputed subordination to the King's authority, the 16th century's moral principles regarding the relationship between the King and the Subjects began to newly highlight the matter of choosing to serve the government, or not. in other words, it became very important for the scholar-officials to abide by certain moral disciplines and obligations when they were going to commence public service in the government or choose to retire from it. They were required to cautiously consider their own abilities, and determine whether they were prepared for the job or not. And most importantly, merely having the abilities was simply not enough. A scholar-official also had to take the personal nature and colors of the King and other vassals into consideration, because they had to act accordingly and provide adequate services at the right place and at the right time. They believed that acting upon these kinds of principles and obligations, was serving the higher cause which was no less than establishing a moral and proper relationship between the King and the subjects. This kind of new highlights in the concept of King-Vassal relationship suggests that there were certain changes made to the political ideologies of this era as well. The most dominant subject matter in the area of political ideologies at the time was, in order to bring the image of an ideal society to reality, whether one should choose to serve the country by having enlisted itself for government service duties, or stay where one was and choose to serve the social ideals through non-governmental, civilian or academical activities. How to utilize governmental power was not a primary issue. Whether to join it or not was. This was the most distinct characteristic of the 16th century's political ideology, compared to the 15th century's one.

3차원 데이터의 Photon Map Rendering 기법에 대한 비교 연구

김종서,조동식,곽훈성 전북대학교 공학연구원 ( 구 전북대학교 공업기술연구소 ) 2002 工學硏究 Vol.33 No.-

The needs about presentations of realistic image have been gradually increased the technical development and importance in the study of computer graphics. With the development of hardware enabling high-resolution image, we suggest to express more natural and accurate image in rendering. This paper presents photorealistic rendering algorithm that embodies accurate image such as photo. With relative evaluation among photorealistic renders that are used in present, we compare base and efficiency and also analyze the interface and application environment. Based on these our comparison, analysis and experimentation, this paper verifies quality and render-speed as important points in renderer. We expect that our experimental results will be used as comparative data in many rendering works.

비정규직 근로자의 고용실태와 개선과제

김훈식,송준호 안양대학교 사회과학연구소 2001 社會科學硏究 Vol.8 No.-

ABSTRACT Since the economic crisis in late 1997, the ratio of non-standard workers to all employees was heavily increased from 41.3% lo 51.7%. Therefore, the interest groups like companies, employees end the government respond positively or negatively. This paper give you some attractive proposals on employment matters of non-standard workers as follower : flexibility of employment period, enlargement of social insurances, practice of equal work & equal wage, abolition of premium in favor of standard workers, design of of employment portfolio, upgrde of working conditions, opportunity of transfer from non-standard worker to standard employees.

야간주차정책에 관한 연구

김광식,박훈립 국민대학교 생산기술연구소 2002 공학기술논문집 Vol.25 No.-

Vehicles must be parked at least temporarily at each end of a vehicular trip before the occupants can partake in their activity. Parking is therefore a fundamental component of urban transportation system. The parking problems however have been got from bad to worse due to high cost of parking improvement. The city governments have formulated the synthetic policies to solve the parking problems without considering the characteristics of night-parking. In this study the direction of the parking policies is presented.