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Meng-Ying Yao,Wei-Hong Zhang,Wen-Tao Ma,Qiu-Hong Liu,Li-Hua Xing,Gao-Feng Zhao 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-
Currently, exosome-enclosed microRNAs (miRs) in exhaled breath have potential for biomarker discovery in patients with pulmonary diseases. This study was performed to investigate the roles of M2 macrophage-derived exosomes expressing miR-328 in pulmonary fibrosis (PF). Microarray-based analysis was used to screen differentially expressed genes (DEGs) and regulatory miRs in PF. The miR-target relationship between FAM13A and miR-328 was confirmed. The expression of FAM13A and miR-328 was measured in PF rats, and gain- and loss-of-function assays were conducted to determine the regulatory effects of FAM13A and miR-328 on PF. In addition, exosomes derived from M2 macrophages were isolated and then cocultured with pulmonary interstitial fibroblasts to identify the role of these exosomes in PF. Furthermore, the effects of M2 macrophage-derived exosomes overexpressing miR-328 on pulmonary fibroblast proliferation and the progression of PF were assessed in vivo. miR-328 might perform a vital function in PF by regulating FAM13A. FAM13A expression was downregulated while miR-328 expression was upregulated in rats with PF, and a miR-target relationship between miR-328 and FAM13A was observed. Additionally, miR-328 overexpression and FAM13A silencing each were suggested to promote pulmonary interstitial fibroblast proliferation and the expression of Collagen 1A, Collagen 3A and α-SMA. Then, in vitro experiments demonstrated that M2 macrophage-derived exosomes overexpressing miR-328 contributed to enhanced pulmonary interstitial fibroblast proliferation and promoted PF. Furthermore, in vivo experiments confirmed the promotive effects of M2 macrophagederived exosomes overexpressing miR-328 on the progression of PF. Collectively, the results showed that
4D 체감형 콘텐츠에 대한 3D Sweetening 연구 실험 절차
맹휘 ( Meng Hui ),송승근 ( Seung Keun Song ),채일진 ( Eel Jin Chae ),김기홍 ( Ki Hong Kim ),박홍식 ( Hong Sik Park ) 한국정보처리학회 2011 한국정보처리학회 학술대회논문집 Vol.18 No.2
3D 입체영상은 영화관에서 시작되어 최근에는 방송, 게임, 테마파크에 이르기 까지 널리 확산되고 있다. 하지만 3D 입체영상에 대한 응용기술의 발전은 입체영상에 쉽게 접근하게 하였음에도 불구하고 3D 입체영상을 시청하는 사용자에 대한 근본적인 연구가 미흡한 실정이다. 이러한 문제로 인하여 3D 입체영상을 접한 대부분의 시청자들이 안구통증, 어지러움 증을 호소하지만 이에 대한 근본적인 대책을 제시하지 못하는 것이 현실이다. 따라서 이러한 입체피로를 해결하기 위한 3D Sweetenig을 다양한 연구방법으로 진행되고 있고, 그 중 4D 체감형 콘텐츠에 대한 감성연구실험 절차를 소개하고자 한다.
A Network Intrusion Detection Model Based on K-means Algorithm and Information Entropy
Gao Meng,Li Dan,Wang Ni-hong,Liu Li-chen 보안공학연구지원센터 2014 International Journal of Security and Its Applicat Vol.8 No.6
Many factors could influence the clustering performance of K-means algorithm, selection of initial cluster centers was an important one, traditional method had a certain degree of randomness in dealing with this problem, for this purpose, information entropy was introduced into the process of cluster centers selection, and a fusion algorithm combining with information entropy and K-means algorithm was proposed, in which, information entropy value was used to measure the similarity degree among records, the least similar record would be regarded as a cluster center. In addition, a network intrusion detection model was built, it could make cluster centers change dynamically along with the network changes, and the model could real-time update the cluster centers according to actual needs. Experiment results show that the improved algorithm proposed is better than the traditional K-means algorithm in detection ratio and false alarm ratio, and the network intrusion detection model is proved to be feasible.
Fractal Structures and Their Computer Simulation in Rapidly Quenched Al - Mn Alloys
Zhang, Meng,Hong, Chunyong,Dai, Hong,Yang, Pinsheng,Tan, Yuxi 대한금속재료학회(대한금속학회) 1999 METALS AND MATERIALS International Vol.5 No.1
The microstructure of rapidly quenched Al-Mn alloys were studied by TEM and SEM. The icosahedral phase in Al-Mn alloys was observed to show various types of fractal morphologies, which may be classified into four kinds: 1) dendritic shape, 2) flower-like shape, 3) granular shape and 4) grain-oscillation shape. After being digitized by a computer, the fractal dimensions (D) of these morphologies were calculated. Based on the traditional diffusion limited aggregation (DLA) model computer simulations were made with two-seeded and many-seeded clusters, which reflect the growing mechanism of some fractal structures in Al-Mn alloys. It is suggested that these fractal structures are formed by many icosahedral particles about 20 nm in size aggregating during the rapid quenching process.
Zhou Meng-jiao,Yang Jia-jie,Ma Ting-yao,Feng Ge-xuan,Wang Xue-lian,Wang Li-Yong,Ge Yu-ze,Gao Ran,Hong-liang Liu,Shan Lin,Kong Lu,Chen Xiao-hong 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-
MYB-NFIB fusion and NOTCH1 mutation are common hallmark genetic events in salivary gland adenoid cystic carcinoma (SACC). However, abnormal expression of MYB and NOTCH1 is also observed in patients without MYB-NFIB fusion and NOTCH1 mutation. Here, we explore in-depth the molecular mechanisms of lung metastasis through single-cell RNA sequencing (scRNA-seq) and exome target capture sequencing in two SACC patients without MYB-NFIB fusion and NOTCH1 mutation. Twenty-five types of cells in primary and metastatic tissues were identified via Seurat clustering and categorized into four main stages ranging from near-normal to cancer-based on the abundance of each cell cluster in normal tissue. In this context, we identified the Notch signaling pathway enrichment in almost all cancer cells; RNA velocity, trajectory, and sub-clustering analyses were performed to deeply investigate cancer progenitor-like cell clusters in primary tumor-associated lung metastases, and signature genes of progenitor-like cells were enriched in the “MYC_TARGETS_V2” gene set. In vitro, we detected the NICD1-MYB-MYC complex by co-immunoprecipitation (Co-IP) and incidentally identified retinoic acid (RA) as an endogenous antagonist of genes in the “MYC_TARGETS_V2” gene set. Following this, we confirmed that all-trans retinoic acid (ATRA) suppresses the lung metastasis of SACC by correcting erroneous cell differentiation mainly caused by aberrant NOTCH1 or MYB expression. Bioinformatic, RNA-seq, and immunohistochemical (IHC) analyses of primary tissues and metastatic lung tissues from patients with SACC suggested that RA system insufficiency partially promotes lung metastasis. These findings imply the value of the RA system in diagnosis and treatment.
( Meng Li ),( Lu Xue ),( Hong-yan Zhu ),( Hongjun Wang ),( Xue Xu ),( Ping-an Zhang ),( Geping Wu ),( Guang-yin Xu ) 대한소화기기능성질환·운동학회 2017 Journal of Neurogastroenterology and Motility (JNM Vol.23 No.3
Background/Aims Gastric hypersensitivity contributes to abdominal pain in patients with functional dyspepsia. Recent studies showed that hormones induced by stress are correlated with visceral hypersensitivity. However, the precise mechanisms underlying gastric hypersensitivity remain largely unknown. The aim of the present study was designed to investigate the roles of corticosterone (CORT) on excitability of dorsal root ganglion (DRG) neurons innervating the stomach. Methods DRG neurons innervating the stomach were labeled by DiI injection into the stomach wall. Patch clamp recordings were employed to examine neural excitability and voltage-gated sodium channel currents. Electromyograph technique was used to determine the responses of neck muscles to gastric distension. Results Incubation of acutely isolated DRG neurons with CORT significantly depolarized action potential threshold and enhanced the number of action potentials induced by current stimulation of the neuron. Under voltage-clamp mode, incubation of CORT enhanced voltage-gated sodium current density of the recorded neurons. Pre-incubation of GF109203X, an inhibitor of protein kinase C, blocked the CORT-induced hyperexcitability and potentiation of sodium currents. However, pre-incubation of H-89, an inhibitor of protein kinase A, did not alter the sodium current density. More importantly, intraperitoneal injection of CORT produced gastric hypersensitivity of healthy rats, which was blocked by pre-administration of GF109203X but not H-89. Conclusions Our data strongly suggest that CORT rapidly enhanced neuronal excitability and sodium channel functions, which is most likely mediated by protein kinase C but not protein kinase A signaling pathway in DRG neurons innervating the stomach, thus underlying the gastric hypersensitivity induced by CORT injection. (J Neurogastroenterol Motil 2017;23:464-476)
Hong Liang Wang,Meng Shi,Xiao Xu,Xiao Kang Ma,Ling Liu,Xiang Shu Piao 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.7
Objective: Two experiments were conducted to determine the content of digestible energy (DE) and metabolizable energy (ME) as well as the apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of crude protein (CP) and amino acids (AA) in barley grains obtained from Australia, France or Canada. Methods: In Exp. 1, 18 growing barrows (Duroc×Landrace×Yorkshire; 31.5±3.2 kg) were individually placed in stainless-steel metabolism crates (1.4×0.7×0.6 m) and randomly allotted to 1 of 3 test diets. In Exp. 2, eight crossbred pigs (30.9±1.8 kg) were allotted to a replicate 3×4 Youden Square designed experiment with three periods and four diets. Two pigs received each diet during each test period. The diets included one nitrogen-free diet and three test diets. Results: The relative amounts of gross energy (GE), CP, and all AA in the Canadian barley were higher than those in Australian and French barley while higher concentrations of neutral detergent fiber, acid detergent fiber, total dietary fiber, insoluble dietary fiber and β-glucan as well as lower concentrations of GE and ether extract were observed in the French barley compared with the other two barley sources. The DE and ME as well as the SID of histidine, isoleucine, leucine and phenylalanine in Canadian barley were higher (p<0.05) than those in French barley but did not differ from Australian barley. Conclusion: Differences in the chemical composition, energy content and the SID and AID of AA were observed among barley sources obtained from three countries. The feeding value of barley from Canada and Australia was superior to barley obtained from France which is important information in developing feeding systems for growing pigs where imported grains are used.
Study on Combustible Classification Method Based on Optimized BP Neural Network
Meng ZHU,Lei ZHAO,Honge REN 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.5
In this paper, combustible classification method based on optimized BP neural network is applied by referring to existing combustible classification method and aiming at the vegetation in the region of Hannuo River in Greater Higgnan Mountains. Combustible classification method based on ground type and stand factor is used according to features of BP neural network model. The results indicate that the classification method proposed in this paper owns high precision and good robustness.
Meng Shi,Zhaoyu Liu,Hong Liang Wang,Chuanxin Shi,Ling Liu,Junjun Wang,Defa Li,Shuai Zhang 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.3
Objective: This experiment was conducted to determine the chemical composition, digestible energy (DE) and metabolizable energy (ME) contents of corn germ meals (CGM) and to develop equations to predict the corresponding energy contents based on the chemical characteristics of individual CGM. Methods: Sixty-six barrows (initial body weight = 51.3±4.6 kg) were allotted to 11 diets including a basal diet and 10 CGM test diets in a completely randomized design. In the test diets, CGM was included in replacement of 30% of the energy-providing ingredients in the basal diet, resulting in a final inclusion rate of 29.1%. Each diet was fed to 6 barrows housed in individual metabolism crates for a 7-d acclimation period followed by a 5-d total but separate collection of feces and urine. Results: Considerable variation was observed in acid-hydrolyzed ether extract, ether extract, ash, calcium (Ca) and total phosphorus contents among the CGM samples. On dry matter (DM) basis, the DE and ME contents of the CGM ranged from 10.22 to 15.83 MJ/kg and from 9.94 to 15.43 MJ/kg, respectively. The acid detergent fiber (ADF) contents were negatively correlated with the DE and ME contents of CGM samples. The best-fit prediction equations for the DE and ME values (MJ/kg DM) of the 10 CGM were: DE = 26.85–0.28 insoluble dietary fiber (%)–17.79 Ca (%); ME = 21.05–0.43 ADF (%)–11.40 Ca (%). Conclusion: The chemical compositions of CGM vary depending on sources, particularly in ether extract and Ca. The DE and ME values of CGM can be predicted based on their chemical composition in growing pigs.