배추 시스테인 단백질 분해 효소 억제 유전자 BCPI-1의 발현과 종자 발아 및 유묘 생장과의 연관성

홍준기,이은영,김정률,양경애,최영주,정우식,김호일,윤대진,이상열,조무제,임체오 Plant molecular biology and biotechnology research 2003 Plant molecular biology and biotechnology research Vol.2003 No.-

Phytocystatins are protein inhibitors of cysteine proteinases of the papain family that have been identified in both monocot and dicot plants. A cDNA encoding a phytocystatin, BCPI-1 (Brassica Cysteine Proteinase Inhibitor-1) has been isolated from Chinese cabbage (Brassica campestris L. ssp. pekinensis) flower buds. Here, we tested whether BCPI-1 transcription is regulated by hormones, and could be involved in regulating cysteine proteinases during and after germination. BCPI-1 was sensitive to exogenous GA_(3) and ABA, which are important factors controlling seed germination, suggesting that the expression of BCPI-1 is hormonally regulated. We introduced a recombinant plasmid containing the full-length BCPI-1 cDNA under the control of cauliflower mosaic virus 35S promoter into rice embryogenic calli using the particle delivery method, and regenerated a number of transgenic rice plants. Constitutively over-expressed BCPI-1 caused changes in overall plant growth and development, including reduced germination and seedling growth. These data support the role of the BCPI-1 in the regulation of endogenous proteinases during both seed germination and subsequent seedling development. Phylocystatins은 papain계열의 cysteine 단백질 분해 효소 활성을 특이적으로 억제하는 억제자로 다양한 식물 종으로부터 분리되었다. 본고에서는, 배추 화아 cDNA library로부터 분리된 phytocystatin인 BCPI-1(Brassica Cysteine Proteinase Inhibitor-1)의 생체 내 기능에 대해 연구하였다. 먼저, BCPI-1 전사체는 발아 조절에 중요한 영향을 미치는 GA₃와 ABA에 의해 예민하게 증가, 혹은 감소되는 반응을 보임으로써, BCPI-1이 식물 호르몬의 영향을 받으며, 특히 발아나 유묘의 생장 조절에 관련이 있음을 알 수 있었다. Particle bombardment 방법을 통하여 BCPI-1을 벼의 배 형성 세포 내에 도입, 재분화 시켜 형질 전환 벼를 생성하였다. CaMV 35S promoter의 조절에 의해 지속적인 BCPI-1 발현을 보이는 형질 전환 벼의 경우, 발아와 유묘 생장이 현저히 지연되었다. 위의 결과들을 바탕으로, BCPI-1이 식물체 내에서 생성되는 cysteine 계열의 단밸질 분해 효소 활성을 조절하여 종자의 발아와 유묘의 생장에 영향을 미치는 것으로 추측한다.

초등학교용 웹 기반 교수-학습 자료 개발 전략

김홍래,정인기,신준식,이대형 춘천교육대학교 과학교육연구소 2001 과학교육연구 Vol.25 No.-

Lead nitrate 投與가 肝細胞增殖에 미치는 影響에 關한 形態學的 硏究

金源式,姜大榮,金弘善 충남대학교 의과대학 지역사회의학연구소 1987 충남의대잡지 Vol.14 No.1

To evaluate the effects of lead nitrate on the hepatic cell proliferation and its mechanism, the one group of male Sprague-Dawley rats were treated with a single dose of lead nitrate (100umol/ kg of body weight) intravenously and the another group were pretreated with phenobarbital (100 mg/kg) intramuscularly once a day for 4 days before lead nitrate treatment for the purpose of research the action mechanism of lead nitrate on liver cell proliferation. Then the relative liver weight, the mitotic activity and the relative amounts of hepatic protein and DNA of each group were compared with those of control group. The results were as follows; 1. The relative liver weight began to increase at 24 hours in the lead nitrate treatment group significantly (p<,0.01), reached the peak at 72 hours, and then decreased after 96 hours. 2. The mitotic activities increased at 48 hours in both experimental groups significantly compared with the control group, reached the peak at 72 hours, and diminished after 96 hours. There was no statistical difference between lead nitrate treated group and phenobarbital pretreated group. 3. The relative amount of hepatic protein showed increment from 24 hours significantly, reached the maximum at 72 hours and then diminished after 96 hours. The relative amount of hepatic DNA in the lead nitrate treated group revealed the maximum value at 96 hours and the phenobarbital pretreated group disclosed the maximal increment at 72 hours. The relative amounts of protein and DNA were more increased in the phenobarbital pretreatment group than those of lead nitrate treatment group.

담관침습을 일으킨 간세포암과 간내 담관암의 감별 진단에 있어 조영제의 급속 정맥 주입방법을 이용한 동맥기 역동적 CT의 유용성 : Value of Arterial Phase Dynamic CT with a Higher Intravenous Injection Rate of Contrast Material

조준식,김대홍 충남대학교 의과대학 지역사회의학연구소 1995 충남의대잡지 Vol.22 No.2

PURPOSE : To evaluate the value of arterial phase dynamic computed tomography (CT) with a highs intravenous (IV) injection rate of contrast material in diagnosing hepatocellular carcinoma (HCC) with bile duct invasion mimicking intrahepatic cholangiocarcinoma (CAC). MATERIALS AND METHODS : Two-phase dynamic CT was performed in 19 patients with pa thologically proven HCCs (n=9) and CACs (n=10). Two-phase images were obtained at 20-50 sec (arterial phase) and 2-4 min (equilibrium phase) after the start of IV injection of contrast material (5 ml/sec, 150 ml). Two-phase images were compared and correlated with pathologic findings. RESULTS : On the arterial phase images, HCCs showed total heterogeneous hyperattenuation in all nine and CACs showed hvpoattenuation in seven and hypoattenuation with peripheral rim enhancement in three. On the equilibrium phase images, all HCCs showed hypoattenuation and capsular enhancement in four. However, all CACs showed hypoattenuation with peripheral or inhomogeneous isodense enhancement. Enhancement patterns of HCCs with bile duct invasion in the arterial phase were significantly different from that of intrahepatic CACs. CONCLUSION : Arterial phase dynamic CT with a higher IV injection rate of contrast material enables HCC with bile duct invasion to differentiate from intrahepatic CAC.

심혈관계를 침범한 Wilms종양 1례

김흥식,강진무,차홍대 啓明大學校 醫科大學 1986 계명의대학술지 Vol.5 No.2

This report is a presentation of a rare case of wilms tumor involved rigth kidney, right renal vein, ingerior vena cava and right atrium seen in a girl of 4 years of age, who presented abdominal distention and hematuria for last 1 month. Ultrasonography of the abdomen revealed dilatation of right renal vein and inferior vena cava and mass iesion in the right atrium was seen on Echocardiogram. A mass of the right upper quadrant which invaded inferior vena cava and right atrium through the right renal vein was found on CT scan of the chest and abdomen, which suggested the wilms tumor of right kidney propagated into the right renal vein, inferior vean cava and right atrium as a tumor thrombosis. A brief review of literature was made.

국가예방접종전산등록체계 개발을 위한 민간의료 기관 하부구조 및 환경 조사연구

이무식,김은영,이건세,이석구,홍지영,김건엽,김대경,신의철,이연경,기몽서,나백주,김영택 한국모자보건학회 2003 한국모자보건학회지 Vol.7 No.2

새마을 所得增進을 위한 리기다 소나무 間伐材의 松脂採取와 經濟性分析

洪性玉,위흡,高大植 全北大學校 1981 論文集 Vol.23 No.-

리기다소나무는 우리나라의 방대한 面積에 植栽되어 最近 多量 生産되고 있으며 앞으로도 그의 生産이 繼續될 趨勢에 있으나 木材的 特性 때문에 利用範圍가 극히 制限되어 있는 實情이다. 이러한 리기다소나무의 間伐材에 대하여 그의 活用方案을 媒索하고자 本 硏究에서는 松脂를 효율적으로 採取할 수 있는 方法 및 그의 經濟性 分析, 組成分의 質的 分析, pulp用材로 利用하기 爲한 處理方法 등에 대하여 硏究하였던 바 그 結果는 다음과 같이 要約될 수 있다. 1. 伐採하기 前 間伐材에서 松脂를 採取할 수 있는 時期는 5月부터 10月까지이며 地域에 따라서 또는 海拔高에 따라서 약간의 差異는 있으나 松脂流出의 最盛期는 7月中임을 알 수 있었다. 2. 松脂는 樹幹의 胸高 部位의 南向에서 採取하는 것이 量的으로 가장 有利함을 알 수 있었다. 3. 採脂溝數를 3개로 하는 것이 1本 1回 實施할 경우 가장 理想的이었으므로 斜溝式採取方法 (Japanese saw method)에 있어서 切傷距離를 약간 크게 하는 것이 좋지 않을까 생각된다. 4. 採脂量은 樹幹의 크기에 따라 크게 影響되며 小徑木에 있어서 Mason方法에 의한 이들 關係를 回歸式으로 나타내면 Y^=1099.24X-3197.60이었다 (단 X=DBH, Y^=採脂量) 5. 松脂의 流出速度는 切傷後 9時間 以內에 總流出量의 約 65% 程度가 流出되었다. 6. 리기다소나무의 松脂에 含有된 monoterpene의 組成은 含量別로 볼 때 α-pinene, β-pinene, β-phellandrene, camphene, Δ-3-carene順으로 되어 있으며 이들중 특히 α-pinene의 含量으로 볼 때에 時期別 樹幹部位別 및 方位別로 큰 差異 없이 대체로 40% 以上이었으므로 리기다소나무의 松脂의 質은 良質의 것임이 判明되었다. 7. 리기다소나무에서 松脂를 採取하므로서 樹幹內에 松脂含量이 減少되거나 材質의 變化는 없었으며 間伐材의 松脂量 減少는 3個月 내지 6個月 동안 野積하므로서 成就할 수 있음을 究明하였다. 8. 리기다소나무의 松脂採取는 大徑木일 때에는 收益性이 대단히 크나 小徑木에서의 採脂는 收益性이 없었다. 다만 遊體勞動力이나 年少者에 의한다면 農山材의 所得增大에 寄與하리라 생각된다. Our country has planted Pinus rigida seedlings in a large scale every year for the last three decades and now they are old enough to be cut for thinning. Actually the thinned timbers are massproduced recently, however, the market value is very low and the utilization of the timber is limited because of its high level of resin content in wood and its twisted wood grains. As an effective utilization of this species the present study investigated possible ways of collection of pine resin (naval store) from the trees before they are cut for thinning, analysed qualitative properties of resin and economical profitability from the resin collection and suggested a pretreatment method to decrease resin content in wood to be used for pulping. The obtained results are as follows: 1) It was found that resin can be collected from May to October and the maximum yield of resin can be obtained in July, though it varied a little with location and elevation. 2) Tapping on the stem southward in breast height resulted in the highest yield of resin. 3) The optimum number of streaks for tapping is thought to be three in every 20㎝ stem length. 4) The resin yield was considerable affected by the size of stem diameter and a regression equation between DBH(X) and resin exudation amount(Y) by Mason's method was calculated as follows; Y=1099.24 X-3197.60. 5) About 65% of total amount of resin yield were exuded within 9 hours after the start of tapping. 6) Components of monoterpene in oleoresin of this species were α-pinene, β-pinene, β-phellandrene, camphene and Δ-3-carene in order from the most to the least. Quality of oleoresin was found to be good in view of its high content of α-pinene (more than 40%) regardless of season, stem part or orientation. 7) Resin content in wood was not decreased, nor wood density changed after resin collection from the stem. A method of decreasing resin in wood was found to pile the thinned timbers in the field for three to six months before they are used for pulping. 8) A good profitability was shown when resin was collected from the trees having large stem but it was not when collected from small stemmed trees. However, if resin is collected by young idle labour in mountain it may be profitable to collect resin even from the small size trees.

근채류와 엽채소류의 종류별 식이섬유함량비교

김성호,홍상식,김대진 동아대학교 생활과학연구소 2000 생활과학연구 논문집 Vol.8 No.-

This study was conducted to determined three kinds of analytical methods for the quantitative structural carbohydrate(dietary fiber, DF; neutral detergent fiber, NDF; crude fiber, CF) of two group of vegetable foods(five kinds of root vegetable, four kinds of leaf vegetable). In root vegetables, DF, NDF and CF were 34.01%, 16.06% and 6.95%, respectively. In leaf vegetables, DF, NDF and CF were 46.10%, 23.96% and 9.27%, respectively. The correlationship of root vegetable were Y=2.44+0.73X. r=0.48(P<0.01) between DF and NDF, and Y=23.96+1.55X, r=0.59(P<0.01) between DF and CF. The correlationship of leaf vegetables Were Y=2.45+0.46X, r=0.61(P<0.01) between DF and NDF, and Y=4.26+0.11X, r=0.55(P>0.05) between DF and CF, respectively. DF/CF ratio of 7.36 in root vegetable, DF/CF ratio of 5.15 in leaf vegetables were calculated, respectively.

기능성 Catechol-O-Methyltransferase 유전자 다형성과 한국인 정신분열병의 관련성

박태원,홍영준,박임순,김형섭,최인미,배수영,윤경식,강대희 大韓神經精神醫學會 2001 신경정신의학 Vol.40 No.4

연구목적 : Catechol-O-Methyltransferase(COMT)는 카테콜아민 신경전달물질의 불활성화를 담당하는 효소로서, 정신분열병과 관련된 후보 유전자로서 알려져 왔다. 최근, 기능성 COMT유전자 다형성과 정신분열병의 관련성이 제기되었는데, 한국인 정신분열병에서도 이러한 관련성이 있는지를 살피기 위해 본 연구를 시행하였다. 방 법 : 정신분열병으로 진단된 환자군 103명과 성별 및 연령별로 1:1 짝짓기 대조군 사이에 유전자형과 대립유전자의 분포를 비교하였다. 또한, 환자군은 공격적 행동, 가족력, 아동기 발병 여부에 따라 두 군으로 나누어 비교하였다. DNA의 다형성 부의를 중합효소연쇄반응에 의해 증폭한 후 ,NlaⅢ제한효소법과 전기영동을 실시하여 유전자형을 판별하였다. 결 과 : 환자군 전체와 대조군 사이에 유전자형과 대립유전자 빈도의 차이가 없었다. 가족력이 있는 환자군을 대조군과 비교하였을 때는, 대립유전자 L을 포함하는 유전자형과 그렇지 않은 유전자형의 분포에 있어 유의한 차이를 보였다(p=0.02, OR=3.9, 95% Cl=1.1∼14.3). 결 론 : 한국인 정신분열병과 기능성 COMT 유전자 다형성 사이에 유의한 관련성이 없었다. 따라서, 기능성 COMT 유전자 다형성이 한국인 정신분열병의 발병에 영향을 주지 못한다. 그러나, 정신분열병 가족력과 기능성 COMT유전자 다형성의 관련성이 시시돠어, 향후 본 연구에서 나타난 방법론적인 문제점을 보완하고 추가 대상자를 확보한 연구가 필요하다. Objectives : Catechol-O-methyltransferase(COMT) is involved in the degradation of catecholamine meurotransmitters and has been investigated as a candidate gene in schizophrenia. Recently, possible relationship between functional COMT gene polymorphism and schizophrenia has been suggested. To address the possible role of functional COMT gene polymorphism in the predisposition to schizophrenia, we carried out an association study in Korean schizophrenic patients and controls. Methods : One hundred and three Korean inpatients diagnosed as schizophrenia and 103 age and sex matched controls were selected as study subjects. Patients were subgrouped into two groups on the basis of history of aggressive behavior, family history of schizophrenia and related disorders, and age at onset. We determined COMT genotypes using PCR of the relevant region followed by digestion with NlaⅢ and electrophoresis. Results : No significant differences of allele and genotype frequencies were noted between patients and controls. However, when patients were categorized by the presence of family history of schizophrenia and related disorders, patients with family history showed almost 4 fold higher frequency of having COMT L allele containing genotype compared to controls(p=0.02, OR=3.9, 9.5% CI=1.10-14.33). Conclusion : Although our results do not support an association between functional polymorphism of COMT gene and schizophrenia overall, the findings suggest an association between functional COMT gene polymorphism and familial schizophrenia. Further studies with large samples are needed to confirm this association.

복벽에 발생한 악성 말초신경초종

김찬규,박성규,홍대식 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

Malignant peripheral nerve sheath tumor (MPNST) tend to develop in deep-seated large nerves, with the sciatic the most commonly affected nerve. We present a case of MPNST that developed at abdominal wall, without definite evidence of von Recklinghausen's disease. The patient was a 41-tr-old man who suffered from left flank and back pain. On physical examinations, 10 x 6 cm-sized mass palpated at left flank of abdomen. Excision and biopsy performed and he was diagnosed with MPNST.