Combined Gene Therapy with Hypoxia-Inducible Factor-1α and Heme Oxygenase-1 for Therapeutic Angiogenesis

Bhang, Suk Ho,Kim, Ju Hee,Yang, Hee Seok,La, Wan-Geun,Lee, Tae-Jin,Kim, Ga Hee,Kim, Hyun Ah,Lee, Minhyung,Kim, Byung-Soo Mary Ann Liebert 2011 Tissue engineering. Part A Vol.17 No.7

<P>Transfection with either hypoxia-inducible factor-1α (HIF-1α) or heme oxygenase-1 (HO-1) gene can induce neovascularization in ischemic tissues. Although expression of transfected HIF-1α gene occurs rapidly, the expressed HIF-1α protein degrades quickly, limiting its therapeutic efficacy. Meanwhile, expressed HO-1 protein does not rapidly undergo degradation, but gene expression occurs a couple of days after transfection, resulting in apoptosis and a delay in angiogenesis in ischemic tissues at the incipient period of HO-1 gene transfection. We hypothesize that combined delivery of HIF-1α and HO-1 gene will enhance antiapoptosis and neovascularization in ischemic tissue compared with HIF-1α or HO-1 single-gene therapy. To test this hypothesis, ischemic mouse hindlimbs were treated with HIF-1α and/or HO-1 gene therapy. The combined gene therapy proved superior to both single-gene therapies, resulting in rapid expression of HIF-1α gene and long-term maintenance of expressed HO-1 protein. The apoptosis in the ischemic region was significantly less, and angiogenic growth factor secretion and angiogenesis were greater in the combined gene therapy than in either of the single-gene therapies. Our results suggest that a combined gene therapy of HIF-1α and HO-1 enhances the transfection of both genes and improves angiogenesis compared with either single-gene therapy.</P>

그람음성 간균의 수술후 상기도 정착에 관한 연구

석민호,김완식,조양자 한양대학교 의과대학 1987 한양의대 학술지 Vol.7 No.1

Upper respiratory colonization and subsequent postoperative pneumonia caused by gram-negative bacilli are increased in patients undergoing surgical procedure. The present study was to investigate the effect of surgical procedure on postoperative respiratory colonization and subsequent infection caused by gram-negative bacilli. Among patients undergoing various major and minor operations at Hanyang university hospital during the months of November and December of 1985, 50 patients were chosen to evaluate the changes in upper respirtory colonization by gram-negative bacilli and receptivity of buccal epithelial cells to Pseudomonas aeruginosa, a common respiratory colonizer and pathogen. Upper respiratory colonization by gram-negative bacilli was significantly increased from preoperative 10% (5/50) to postoperative 29% (13/45). Klebisiella pneumoniae was the most frequent postopertative isolate (4 strains). Viability of buccal epithelial cells was decresed postoperaively (P<0.05). Receptivity of buccal epithelial cell to Pseudomonas aeruginosa was increased postoperatively (P<0.001) in mean numbers of attached bacteria per cell and in numbers of cells with minimal numbers of attached bacteria and these two parameters were extremely correlated (=0.999). Upper respiratory colonization rate of gram-negative bacilli and increase in receptivity of buccal epithelial cells to Pseudomonas aeruginosa was significantly greater in patients whose operation took more than 2 hours than in those whose operation lasted less than 2 hours (P<0.001). Data obtained in the present study suggest that changes in surface binding properties of upper respiratory epithelium may be an important factor in predisposing to postoperative respiratory infection by gram-negative bacilli.

補强 開口部를 갖는 合成보의 擧動에 관한 硏究

崔山鎬,梁永晟,金圭石,金錫中,金弼中 江原大學校 産業技術硏究所 1991 産業技術硏究 Vol.11 No.-

A model for the strenghth of composite beams with reinfforced web openings is presented. All of theh models are based on the static theorem of ultimated strength of reinforced rectangular perforated composite beam and is compared to the test.

Cone-beam CT를 이용한 제II급 부정교합 아동의 상기도 형태에 관한 3차원적 연구

홍지숙,김대성,오경민,김윤지,이규홍,박양호 대한치과교정학회 2010 대한치과교정학회지 Vol.40 No.3

Objective: The aim of this study was to evaluate the volumes and areas of the upper airways in children with Class II malocclusion, using three dimensional cone-beam computed tomography (CBCT) and to compare the volumetric and cross-sectional measurements and cephalometric variables to investigate possible relationships between the upper airway and facial morphology. Methods: CBCT scans were obtained from 37 subjects (17 boys and 20 girls; average age, 11.02 years). The upper airway volumes and areas were measured, and compared with cephalometric variables. Results: The area of the PNS-posterior plane (SPP) was significantly smaller in the Class II malocclusion group (p < 0.05). Also, the volumetric and cross-sectional measurements were lower in Class II than in Class I malocclusion groups, although the differences were not significant between the two groups (p > 0.05). The Class II malocclusion group showed significantly smaller values of PFH, mandibular body length, pog to N perp and showed larger values of FMA, ANB, and facial convexity than the Class I malocclusion group. The volume of the upper airway in front of PNS point (WN) showed negative correlation with ANB (p < 0.05). Conclusions: The Class II malocclusion group had a narrower upper airway associated with a decreased posterior facial height and a divergent growth pattern than the Class I malocclusion group. 성장기 아동의 상기도 협착은 두개안면 성장에 영향을 미칠 수 있다. 본 연구는 3차원 cone-beam computed tomography (CBCT)를 이용하여 제II급 부정교합 아동의 상기도 부피 및 단면적 계측치와 측모두부계측치를 비교하여 상기도와 안면 형태와의 연관성을 알아보고자 하였다. 37명의 아동(남자 17명, 여자 20명, 평균 연령 11.02세)의 CBCT를 촬영하였다. 연구 결과, 상기도의 PNS-posterior plane에서의 단면적(S_(PP))은 제II급 부정교합군에서 유의하게 작았다 (p < 0.05). 상기도 부피 계측치는 제II급 부정교합군에서 제I급 부정교합군보다 작았으나, 두 군 간 통계적으로 유의한 차이는 없었다. 제II급 부정교합군은 후안모고경(PFH), mandibular body length (p < 0.05), Pog to N perp (p < 0.01)에서 제I급 부정교합군보다 유의하게 작은 값을 보였고, FMA (p < 0.05), ANB, facial convexity (p < 0.01)는 유의하게 큰 값을 보였다. 제II급 부정교합군의 PNS 전방의 상기도 부피(W_(N))는 ANB difference 각도와 음의 상관관계를 보였다 (p < 0.05). 제II급 부정교합 아동은 상대적으로 좁은 상기도 부피를 가지며 이에 따른 감소된 후안모고경(PFH)과 하악골의 divergent한 안면 성장 양상을 보였다.

韓國의 精神分裂症患者와 손잡이(Handedness)에 대한 硏究

朴錫浩,楊秉換,高珍富 서울대학교 의과대학 정신과학교실 1982 精神醫學報 Vol.6 No.8

Carbonic Anhydrase Inhibitors가 Lipopolysaccharide에 의해 유도된 골흡수에 미치는 영향

박양호,차경석 대한치과교정학회 1994 대한치과교정학회지 Vol.24 No.1

To study bone resorption mechanism effect of LPS on the Ca release from fetal rat ulnae and radii, and effects of carbonic anhydrase inhibitors on the LPS-induced bone resorption in organ culture were studied. Ulnae and radii were removed from 19 days old fetal rats, prelabelled by subcutaneous injection of 200 Ci CaCl₂into their mother on the 17th day of gestation. Radioactivities of Ca released into media were determined after 24, 48 and 72 hours. Effects of LPS and carbonic anhydrase inhibitors were observed by the ratio of % release Ca between paired control and experimently group. The observed results were as follows : 1. LPS(1㎍/㎖) supplemented in media for 72hours increased the Ca release significantly after 48 and 72 hours of culture and LPS(10㎍/㎖) increased the Ca release significantly after 72 hours of culture. 2. LPS-induced Ca release was not inhibited significantly by 1mM sulfanilamide but inhibited significantly by 10mM significantly after 48 and 72 hours of culture. 3. LPS-induced Ca release was not inhibited significantly by 0.1mM dichlorphenamide but inhibited significantly by 10mM dichlorphenamide after 48 and 72 hours of culture. 4. LPS-induced Ca release was not inhibited significantly by 1mM acetazolamide but inhibited significantly by 5mM acetazolamide after 72 hours of culture.

果實·菜蔬類의 窒酸鹽 및 亞窒酸鹽含量

金章亮,朴榮浩,千石祚 釜山水産大學校 1984 釜山水産大學 硏究報告 Vol.24 No.1

果實·菜蔬類의 窒酸鹽 및 亞窒酸鹽의 含量을 밝히기 위하여 배추, 양배추, 엇갈이배추, 상치, 시금치, 가지, 오이, 양파, 당근, 파 및 무 등 11종류의 菜蔬類와 사과(紅玉, 골든, 골든델리셔스, 陸奧), 감(富有, 堂上蜂屋), 포도(Tanored, Seibel 9110)등 10종류의 果實類를 食料로 하여 生體의 各部位別로 나누어 그 含量을 調査, 檢討하였는데, 그 結果는 다음과 같다. 1. 배추, 양배추, 엇갈이배추, 상치 및 시금치등의 菜蔬類에 있어서는 全般的으로 葉柄部의 窒酸鹽含量이 다른 部位에 비하여 높은 傾向을 나타내었으며 특히, 시금치의 葉柄部는 2050ppm으로 가장 높은 含量을 나타내었고, 이어, 엇갈이배추, 상치, 배추, 양배추의 順이었다. 2. 가지 및 오이등의 果菜類에 있어서는 果皮部가 果肉部보다 높은 窒酸鹽含量을 나타내었으마, 그 量은 菜蔬類에 비하여 全般的으로 적어서 오이에서는 108ppm, 가지는 169ppm이였다. 3. 양파, 당근, 대파 및 무 등의 根菜類에 있어서는 일반적으로 根部보다는 葉柄部가 있는 上端部의 窒酸鹽含量이 높은 傾向을 나타내었다. 종류별로는 무의 含量이 가장 높아 1101ppm을 나타내었으며, 이어, 당근 대파, 양파의 順이었는데, 특히, 양파의 含量이 적어 16ppm에 지나지 않았다. 4. 菜蔬類의 亞窒酸鹽含量은 全般的으로 낮아 0.1∼3.0ppm였으며, 菜蔬類에 있어서는 葉柄部의 含量이 높은 경향을 보였으나 果菜類 및 根菜類에 있어서는 部位別에 따른 뚜렷한 차이를 찾아볼 수 없었다. 5. 果實類의 窒酸鹽含量에 있어서는 全般的으로 菜蔬類에 비하여 월등히 낮은 含量을 나타내었으며, 果皮部가 果肉部에 비하여, 높은 경향을 보였다. 試料果實中에서는 사과의 含量이 가장 높았으며, 이어, 배, 포도, 감의 순이었고 사과에 있어서는 紅玉, 골든 및 골든델리셔스등은 31∼32ppm으로 비슷한 含量이었으나, 陸奧는 5ppm정도로 월등히 적은 含量을 나타내었다. 6. 果實類의 亞窒酸鹽含量은 全般的으로 극히 낮은 편이었으며, 특히, 배의 경우는 거의 검출되지 않았다. 試料果實中 감의 含量이 가장 높아 紅枾의 경우에 있어서는 窒酸鹽含量과 거의 비슷한 2.4ppm으로 나타났다. It has been known that nitrates and nitrites accelerate not only the corrosion of tinplate, but also the formation of carcinogen. This study was carried out to elucidate the distribution of nitrates and nitrites from the fruits and vegetables by portions. Nitrate contents in the petioles of vegetables were higher than those of other portions. Especially, nitrate content in the petiole of spinach was the highest as 2050 ppm. There were no appreciable differences in the contents of nitrites of the vegetables by portions. Nitrite contents of the vegetables were less than 1.0 ppm except spinach and chininese cabbage for 2.2-3.0 ppm. Nitrate contents in the fruits were generally low in comp arison with those of vegatables. Nitrate contents of fruit skins were more than those of other portions. On the other hand, notrites were not detected in pears.

1930年代 農民小說의 두 樣相 : 「흙」과 「故鄕」을 中心으로

崔正錫,吳養鎬 대구효성가톨릭대학교 1980 연구논문집 Vol.22 No.1

간질발작을 주 증상으로 하는 회백질 이소증을 동반한 Schizencephaly 1례

신석호,양일권,김제헌 大韓神經精神醫學會 1991 신경정신의학 Vol.30 No.4

We experienced a case of schizencephaly with heterotopic gray matter that was only manifested a seizure attack. The clinical manifestation of this patient was only seizure without mental retardation, which was controlled by anticonvulsants. The term schizencephaly was introduced by Yakovlev and Wadsworth in 1946 to describe bilatral, nearly symmetrical and full-thickness clefts, which were characterized by an infolding of cortical gray matter along the clefts. The most widely accepted theory for the pathogenesis of this anomaly is the failure of formation of a segment of the germinal matrix or segmental failure of neuronal migration. Heterotopic gray matter is frequently found within and near the cleft. Patients who have these condition present clinically with seizures and developmental delay, and abnormal motor skills are noted in the more severly affected cases. Recognition is important for planning proper treatment and for genetic counseling.

교감신경자극시 Norepinephrine에 대한 신혈관 반응에 변화

이석호,민영기,양훈모 순천향의학연구소 2002 Journal of Soonchunhyang Medical Science Vol.8 No.1

To determine to effect of angiotensin Ⅱ(ANG Ⅱ)-induced hypertension on the renovarcular respones to norepinephrine (NE), six anesthetized dogs instrumented for monitoring of renal blood flow (RBF) were subjected to step increases every 2 minutes in the rate of norepinephrine infusion into the renal artery during 1) control condition(C), 2) servocontrol of renal arterial pressure (RAP) at levels expected to be achieved during acute captopril administration(C+S), 3) acute captopril administration(CEI), 4) angiotensin Ⅱ infusion simultaneously with captopril at concentrations which restored RAP toward control (CEI+A), and 5) servocontrol of RAP during captopril and angiotensin Ⅱ (SEI+A+S). Whether the plasma levels of ANG Ⅱ were allowed to increase(C, C+S) or not (CEI+A, CEI+A+S) with NE infusion, there were no differences in RBF respones to NE infusion between C and C+S and between CEI+A and CEI+A+S, which implies that RAP does not play an important role in modulating the renovascular response to NE. However, when captopril was administered without ANG Ⅱ replacement (CEI), RBF induced severe renal ischemia at much lower rates of NE infusion than occurred when the sufficient concentration of ANG Ⅱ were present in the plasma. Since this enhanced sensitivity to NE did not occur at RAP's lower than those achieved during acute captopril administration once ANG Ⅱ was present in the plasma in sufficient concentrations, it is likely that ANG Ⅱ exerts a protective effect on the kidney during renal adrenergic stimulation through a mechanism other than increasing systemic arterial pressure.