Dual aptamer-functionalized silica nanoparticles for the highly sensitive detection of breast cancer

Jo, H.,Her, J.,Ban, C. Elsevier Applied Science 2015 Biosensors & bioelectronics Vol.71 No.-

In this study, we synthesized dual aptamer-modified silica nanoparticles that simultaneously target two types of breast cancer cells: the mucin 1 (MUC1)(+) and human epidermal growth factor receptor 2 (HER2)(+) cell lines. Dual aptamer system enables a broad diagnosis for breast cancer in comparison with the single aptamer system. The dye-doped silica nanoparticles offer great stability with respect to photobleaching and enable the accurate quantification of breast cancer cells. The morphological and spectroscopic characteristics of the designed Dual-SiNPs were demonstrated via diverse methods such as DLS, zeta potential measurements, UV-vis spectroscopy, and fluorescence spectroscopy. Negatively charged Dual-SiNPs with a homogeneous size distribution showed robust and strong fluorescence. In addition, Dual-SiNPs did not affect cell viability, implying that this probe might be readily available for use in an in vivo system. Through ratio optimization of the MUC1 and HER2 aptamers, the binding capacities of the Dual-SiNPs to both cell lines were maximized. Based on Dual-SiNPs, a highly sensitive quantification of breast cancer cells was performed, resulting in a detection limit of 1cell/100μL, which is significantly lower compared with those reported in other studies. Moreover, the developed detection platform displayed high selectivity for only the MUC1(+) and HER2(+) cell lines. It is expected that this valuable diagnostic probe will be a noteworthy platform for the diagnosis and prognosis of breast cancer.

印刷媒體接觸에 대한 時間工學的 硏究

朴許植 청주대학교 사회과학연구소 1983 한국사회과학연구 Vol.1 No.-

The purpose of this study is to search for the impacts of time factors to the printed media contact behavior under perspectives of time-engineering. It is very important for the audience to allocate its leisure time(time budget) on certain acts choosen from various alternative acts. Here, leisure time is defined as the total time except non-disposable time which is essential to maintain one's life. For this purpose, the results of the survey research on the national people's lifecycle(time-allocation) conducted by the Korean Broadcasting System in 1981 were used, reanalyzed and reinterpreted as a basic data. The study findings are summerized as follows; 1) The national people's behavior rate allocated to newspapers and magazines/books (newspaper contact behavior rate; 27.4%, magazine/books contact behavior rate; 28.2%) is much less than that of radio and television. And the national audiences' average media. exposure time to these printed media is also very low. The report on the Survey Research shows on average media exposure time of 36 minutes to newspapers, one hour and five minutes to magazines/books. 2)According to sex, only male respondents show some significant newspaper biased behavior trend. But significant differences in average exposure time of newspapers and. magazines/books are not found by sex. 3) Analyzing the time band which shows behavior rate above 3% viewing from time engineering perspectives, while newspapers are considered as morning-reading type (media mainly read in the morning), magazines/books are considered as night-reading type (media mainly read during the night) on the national bases. By sex, to the male readers, newspapers are morning-reading type, magazines/books are night-reading type. On the contrary, female readers show day-reading type behavior(female readers read. often newspapers during the day) relating to newspapers and night-reading type behavior in magazines/books. 4) By age people between 20s and 40s show the heaviest media exposure to newspapers and magazines/books, in additon, their amount of time spent on these media is the most compared with the other age groups. 5) Judging from the printed media contact behavior rate, male respondents between 30s-50s show a high newspaper-biased media behavior, and female respondents in their 20s show the highest book/magazine-biased media behavior. 6) According to the respondent's occupation, the greatest differences are found between the occupation groups of self manager, saler, servicer, technicians, engineers, business manager, professionalist, free occupation and others and the occupation groups of agricultural-fisheries, housewives, and students in newspaper reading behavior in terms of behavior rate. 7) Newspapers show greater differences between respondents who have a job and those who do not job in behavior rate, but these no difference in average media exposure. 8) So the factor of education has a great impact both on the printed media contact behavior rate and the average amount of media exposure time. A higher educational level will result in increased printed media contact behavior rates or average amount of media exposure. 9) Regional analysis as a socio-cultural classification is very important in the printed media contact behavior rate. Greater differences are shown in the behavior rate between the largest, middle and small sized city and county or town, but no differences are found in amount of time by region. Summerizing the results mentioned above, various socio-cultural factors have a great influence on the printed media contact behavior rate, but have little impact on the amount of time required for such behavior. This is on important finding which must be considered in any survey research study on reading patterns and amount of reading time. Hence, beyond existing one-dimensional research study methods on the amount of reading time, future research on the amount or reading time must be conducted by relating the amount of reading time as well as the reading time band, a study of time engineering perspectives, because reading behaviors are also limeted by time among various alternative behaviors within a given 24-hour day.

초등학교 1학년 통합단원 "함께하는 한가위" 재구성 실행연구

신혜리 ( Her Yee Shin ),홍영기 ( Young Ki Hong ) 한국통합교육과정학회 2010 통합교육과정연구 Vol.4 No.1

본 연구는 초등학교 1학년 2학기 ``바른 생활``, ``슬기로운 생활``, ``즐거운 생활``과의 통합 단원인 ``함께하는 한가위``를 통합적 관점에서 재구성하여 실행한 연구이다. 34명의 초등학교 1학년 학생을 대상으로 재구성한 통합단원을 교사연구자가 실행하는 과정에서 일어나는 교사의 교육과정 재구성에 대한 인식과 학생들의 수업태도 및 참여의 변화를 이해하고 묘사하는데 초점을 두었다. 통합단원의 재구성은 한 차시의 내용에 ``바른 생활``, ``슬기로운 생활``, ``즐거운 생활``과의 내용을 학생들의 생활과 밀접한 통합 활동으로 재구성하여 통합교과가 한 단원 당 2주간 배정된 시량인 총 22차시로 재구성하여 적용하였다. 이를 위해 교수·학습과정안, 학생용 워크북, 교사용 수업자료 등을 개발하였다. 자료 수집은 연구일지, 참여관찰과 비구조화된 면담, 실행과정에서의 실행일지, 학생들이 작성한 학습일지, 동영상 및 사진촬영 등을 통해 이루어졌다. 연구를 통해 학생들은 학습에 있어 함께하는 방법을 알고, 학교는 즐거운 배움터라는 인식을 하였으며, 교사의 변화로는 학습자 중심 교육에 대한 인식변화, 학생을 바라보는 관점의 변화, 교육과정 재구성 시도에 대한 두려움의 극복 등이 있었다. This is an action research on restructuring the integrated unit, ``Sharing together Hangawee`` that consisted the same theme across three integrated subjects. The purpose of this study was to describe and understand the changes of the action researcher`s awareness of curriculum restructuring and of the student participants` attitudes toward learning and classroom participation. Restructuring the integrated unit was mainly done by the way of designing activities closely related with students` real life and three integrated subjects of the first grade. The unit consisted of twenty-two class periods which was the standardized class period for one unit. For the implementation, teaching materials such as lesson plans, student workbook, powerpoints, hand-outs were developed and applied. Based on the research, the changes of the student were ``knowing how to cooperate with peers`` and ``recognizing school as a place of enjoyable learning``. The action researcher changes her paradigm on student-centered instruction and on the viewpoints of the student as a learner. In addition, her ability of curriculum restructuring was improved.

심리기술훈련이 경쟁 상태 불안과 운동 수행에 미치는 영향

주동엽(Joo Dong-Yeup),범대진(Bhum Dae-Jin),허재호(Her Jeo-Ho) 한국체육과학회 2000 한국체육과학회지 Vol.9 No.2

The purpose of this study was to investigate the effects of psychological skills training on competitive state anxiety and sport performance. The participants were 76 physical education students (40 males, 36 females) ranging in age from 18 to 27 from the Dong-shin University in Naju city. In order to measure competitive state anxiety, the Competitive State Anxiety Inventory-2 (CSAI-2) was used. It was composed of 27 questions covering cognitive, physical instabilities and self-confidence respectively with 9 questions. To measure sports performance, the participants were performed golf putting. Prior to performing the golf putting, the participants completed the CSAI-2 to measure competitive state anxiety and a test of golf putting. After pre-test, the participants divided randomly four different groups to received 16 weeks psychological skills training. One of the groups was a control group and the others were three experimental groups. Three experimental groups were performed a 16 weeks psychological skills training program by using the Progressive Muscle Relaxation and the Imagery Training. The control group was not contacted in any way during the 16 weeks. After 16 weeks, the participants were re-tested using the same methods as in the pre-test to examine the effects of the psychological skills training. The results were as follows: 1) The psychological skills training programs were effective in reducing anxiety levels of the participants. Three experimental groups were meaningful for reducing his/her cognitive anxiety. Its were also effective for lessening his/her physical instability and improving self-confidence. 2) The psychological skills training programs were effective in improving the golf putting scores. The score gap between the control group and three experimental groups were particularly large. This means that the psychological skills training programs were affected to his/her competitive state anxiety, thereby contributing to improving scores in the Golf putting' performance.

A Study of Viewers' Responses to Luxury Haul Videos on YouTube

( Yusun Her ),( Jaehoon Chun ) 한국의류학회 2020 한국의류학회지 Vol.44 No.4

This study examined how viewers perceived luxury haul videos, what factors made them experience vicarious pleasure and gain relative deprivation when watching them. This study conducted an interview with 20 viewers of luxury haul videos. The content obtained from the interview was analyzed by classifying it into academic terms. Cases of vicarious pleasure arose when viewers had sufficient empathy when watching videos, which was intensified by YouTubers' behavior and visual stimulation in videos. A person's relative deprivation due to her financial capabilities or appearance occurred when she compared herself with YouTubers. The more viewers perceived luxury haul as a source of information, the more they felt relative deprivation; in addition, the more they perceived it as entertainment, the more they felt vicarious pleasure. Viewers who had a high level of involvement in luxury goods tended to feel vicarious pleasure; however, viewers with a high level of involvement in YouTubers tended to feel relative deprivation. This study helps luxury brands and YouTubers understand viewers' perception of their products and programs as well as helps in the development of fashion haul videos that are beneficial to viewers.

독서 상담을 위한 도서 선정과 질문 구성

김영란(Kim Young-ran),허지은(Her Ji-eun) 국어교육학회 2010 국어교육연구 Vol.47 No.-

The purpose of this study is to provide counsellors with helpful advice for counselling with book-reading; First of all, we review the psychiatric issues that challenge Korean pre-schoolers and adolescents (up to the age 24) and the issues that can be aided by book-reading counselling. Secondly, we develop a set of criteria to select relevant books for the counsellee, and this criteria should be dependent on the major issues of the counsellee. Thirdly, we also suggest a package of questions which are generated based on the principles of psychotherapy and teaching of reading. The series of questions should be split into 3 parts: pre-reading, during-reading, and post-reading. The questions need to guide counsellee to find characters or events in a book for identification or projection, to accommodate himself/herself to indirect experience through book-reading, to help himself/herself undergo catharsis, and to facilitate himself/herself to integrate new learning from the reading with his/her own problem, so (s)he can gain new insight into solving his/her own psychiatric issues. We plan to conduct follow-up research which will explore counsellees with multicultural backgrounds and apply to them the principles and method this current study suggests. We will provide book-reading counselling to them and the course of counselling will be researched quantitatively and qualitatively as well. The course of action between counsellors and counsellees will be thoroughly reviewed qualitatively and the efficacy of the program will be examined quantitatively.

Tumebacillus luteolus sp. nov., isolated from soil

Her, Jihee,Srinivasan, Sathiyaraj,Lee, Sang-Seob Microbiology Society 2015 International journal of systematic and evolutiona Vol.65 No.11

Transflective fringe-field switching liquid crystal display without any retarder

Her, Jung Hwa,Shin, Suck Jae,Lim, Young Jin,Park, Kyoung Ho,Lee, Joun Ho,Kim, Byeong Koo,Lee, Gi-Dong,Lee, Seung Hee The Optical Society 2010 Optics express Vol.18 No.22

<P>Conventional transflective liquid crystal displays (LCDs) with single cell-gap requires optical compensation films or patterned retarders to balance the optical path-length difference between transmissive and reflective regions. In this paper, novel single cell-gap transflective LCDs driven by fringe electric field without using the compensation film or in-cell retarder have been proposed. The liquid crystal director is aligned parallel to analyzer but makes an angle of 45° with respect to analyzer in reflective region. In addition, the surface pretilt angle in the reflective region is controlled by vertical field and polymerization of an UV curable reactive mesogen at the same time and thus, the effective cell retardation in the reflective region becomes smaller than that in transmissive region. Consequently, without using any compensation film or in-cell retarder, the single cell-gap and single-gamma transflective LCD with high performance is realized.</P>

SiN_<i>x</i>Prepassivation of AlGaN/GaN High-Electron-Mobility Transistors Using Remote-Mode Plasma-Enhanced Chemical Vapor Deposition

Her, Jin-Cherl,Cho, Hyun-Jun,Yoo, Chan-Sei,Cha, Ho-Young,Oh, Jae-Eung,Seo, Kwang-Seok IOP Publishing 2010 Japanese journal of applied physics Vol.49 No.r4

5S rRNA Sequence of Trimorphomyces papilionaceus

Her, Yong,Kang, Young-Won,Park, Yong-Ha,Jung, Hack-Sung The Microbiological Society of Korea 1992 미생물학회지 Vol.30 No.6

The sequence of the cytoplasmic 5S-rRNA from Trimorphomyces papilionaceus, a basidiomycetous yeast, was determined by the direct chemical method for sequencing RNA and compared to known 5S rRNA sequences of 19 basidiomycetous fuungi. There were 26 nucleotide differences between T. papilionaceus and Tremella mesenterica both of which belong to the Tremellaceae of the Tremellales. Based on Knuc values, the closest fungus was Tilletiaria anomala, another basidiomycetous yeast which belong to the Sporbolomycetaceae of the Sporobolomycetales. T. papilionaceus did not show any significant phylogenetic relationship with other fungi.