Typhoid Vi Capsular Polysaccharide Vaccine의 임상시험에 관한 연구

허준호,김행미,이건수 慶北大學校 醫科大學 1997 慶北醫大誌 Vol.38 No.1

목적 : 장티푸스 예방 접종 중 살모넬라 Ty2 균주에서 강력한 항원성을 갖는 Vi 협막성 다당질항원을 분리 정제한 백신이 국내에서 개발된 바 그 효능과 부작용에 대하여 알아보고자 이 연구를 시행하였다. 대상 및 방법 : 1993년 4월부터 1995년 4월까지 주식회사 녹십자의 타이포박스®를 경남 울산군소재 애리원생 86명을 대상으로 접종 전과 접종 후 1개월, 3개월 및 12개월에 혈액 3㎖를 채취하여 혈청을 분리하고, 이를 녹십자 종합연구소에 의뢰하여 수동혈구응집반응법(passive hemagglutination)으로 Vi 항체가를 측정하였다 결과 : 예방 접종 후 Vi 항체의 양성율은 1개월에 96.5%(검사 대상 86례 중 83례), 3개월에 96.5%(86례 중 83례), 12개월에 96.4%(86례 중 81례)였으며 부작용으로는 경미한 국소 발적 반응과 경한 통증이 각각 5례, 경한 발열이 2례에서 있었다. 결론 : Vi 협막성 다당질 항원을 분리 정제한 타이포박스®는 접종 후 높은 항체가와 경미한 부작용을 나타내어 장티푸스 예방 접종 주사로서의 임상적 가치가 높다고 사료된다. Purpose : Recently, Vi capsular polysaccharide from Salmonella typhi Ty2 has been purified and manufactured as a vaccine(Typhovax®, Korea Green Cross) in Korea. To evaluate the safety and immunogenicity of Vi capsular polysaccharide vaccine this study was performed. Methods : A single dose of 0.5㎖(25㎍) was injected subcutaneously to 86 persons aged 8-44 years. Anti-Vi antibody titers were measured by passive hemagglutinarion test before and 1, 3, and 12 months after vaccination. Results : The rate of seroconversion was 96.5% at 1 month after vaccination. The antibody titers were sustained for at least 12 months. Transient local reactions, including pain and erythema, were noted in 5 cases(5.8%) and mild fever was developed in two cases(2.3%). Conclusions : To see whether this acquired level of Vi antibody titers protects infection from typhoid fever and the elevated level of Vi antibody titers persists, further clinical and epidemiological studies would be required.

生活선的哲學味道

( Jun Heng Sun ),( Yue Li ),( Ren Gu Xi ) 동아시아불교문화학회 2012 동아시아불교문화 Vol.11 No.-

“生活선”是中國淨慧法師在1991年提出來,在1993年거辦第一屆生活선夏令營的時候,正式推出 “生活선”理念。淨慧法師的生活선主旨是:선與生活是同時幷存的,有生活就有선;就是要讓선回到生活中;就是希望佛法能구淨化人心,淨化社會;爲了讓更多的人安身立命,要普及佛法,利用선接引當機;佛敎只有落實到生活的各個方面,才能진正變成自己的血肉和靈魂。要在心態上時時觀照自己,時時把信仰和言行打成一片,加以修行。我們認爲,“生活선” 將佛敎深奧的道理現實化了,具有可操作性,蘊含了豊富的哲學辯證法,閃朔著智慧的光芒。下面僅就當下與未來、點滴與善果、理念與修行、塵世與超越四大方面加以說明。 Living Zen was proposed by the Chinese Zen Master Jing Hui in 1991, the main idea are: Zen and life side by side, there is life, there is Zen; We should make Zen back to life; Buddhism can purify the mind, purify society; In order to allow more people to settle down, the popularity of Buddhism, Zen should be implemented in all aspects of life, can truly become our own flesh, blood and soul. Man should always observe oneself, beliefs and behavior from Buddhism. We believe that the “Living Zen” is an important truth of Buddhism today and it contains a wealth of philosophical dialectics, for example, the four relations between the present and the future, the small and the big, idea and practice, the actual and beyond, shining the lights of wisdom.

歐陽修的儒家君子思想觀

손군항 ( Jun Heng Sun ),진성진 ( Xingchen Chen ) 퇴계학부산연구원 2016 退溪學論叢 Vol.27 No.-

“唐宋八大家”之一的歐陽修, 不僅在政治上建樹頗多、文學上령開一代文風, 同時用獨特的視角和風格對儒家君子思想進行了硏究:明確界定了“君子”行義的本質, 完美勾勒出君子的品行, 認眞踐行君子人格, 使其保持永久魅力, 幷從“君子之德”、“君子之修”、“君子之交”、“君子之勇”、“君子之樂”五個方面完整闡釋了君子的認識。 Ouyang Xiu(歐陽修), one of Chinese Eight Famous scholars between Tang and Song Dynasties(唐宋八大家), was not only on the political achievements and a lot of literature, but also opened a new style of writing with unique perspective. At the same time, he studied the thoughts of Confucian (儒家)gentleman(君子)------clearly define the nature of "gentleman", perfectly outline of the gentleman``s character, seriously practice of gentleman personality, keep the permanent charm of "gentleman". From the five aspects of "morality of gentleman", "a gentleman training", "association of gentleman", "gentleman’s bravery", "entertainment of gentleman ",Ouyang Xiu gave us a complete explanation of the gentleman.

The Design of Fingerprint Identification System based on Improved Binarization Algorithm

Zhu Heng-jun,Guo Ying,Zhang nan-nan,Liang Hong 보안공학연구지원센터 2014 International Journal of Security and Its Applicat Vol.8 No.6

Modulation of Drug Resistance in Ovarian Cancer Cells by Inhibition of Protein Kinase C-alpha (PKC-α) with Small Interference RNA (siRNA) Agents

Zhao, Li-Jun,Xu, Heng,Qu, Jun-Wei,Zhao, Wan-Zhou,Zhao, Yi-Bing,Wang, Jin-Hua Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8

Objective: To determine whether silence of $PKC-{\alpha}$ expression by small interference RNA (siRNA) might regulate MDR1 expression and reverse chemoresistance of ovarian cancer. Methods: We measured gene and protein expression of MDR1 and $PKC-{\alpha}$ in ovarian cancer cells and assessed their correlation with cell drug resistance. We also examined whether blocking $PKC-{\alpha}$ by RNA interference (RNAi) affected MDR1 expression and reversed drug resistance in drug sensitivity tests. Results: The drug resistance cell lines, OV1228/DDP and OV1228/Taxol, had higher gene and protein expression of MDR1 and $PKC-{\alpha}$ than their counterpart sensitive cell line, OV1228. SiRNA depressed $PKC-{\alpha}$ gene protein expression, as well as MDR1 and protein expression and improved the drug sensitivity in OV1228/DDP and OV1228/Taxol cells. Conclusion: These results indicated that decreasing $PKC-{\alpha}$ expression with siRNA might be an effective method to improve drug sensitivity in drug resistant cells with elevated levels of $PKC-{\alpha}$ and MDR1. A new siRNA-based therapeutic strategy targeting $PKC-{\alpha}$ gene could be designed to overcome the chemoresistance of ovarian cancer.

Increased Serotonin Signaling Contributes to the Warburg Effect in Pancreatic Tumor Cells Under Metabolic Stress and Promotes Growth of Pancreatic Tumors in Mice

Jiang, Shu-Heng,Li, Jun,Dong, Fang-Yuan,Yang, Jian-Yu,Liu, De-Jun,Yang, Xiao-Mei,Wang, Ya-Hui,Yang, Min-Wei,Fu, Xue-Liang,Zhang, Xiao-Xin,Li, Qing,Pang, Xiu-Feng,Huo, Yan-Miao,Li, Jiao,Zhang, Jun-Feng Elsevier 2017 Gastroenterology Vol.153 No.1

<P><B>Background & Aims</B></P> <P>Desmoplasia and poor vascularity cause severe metabolic stress in pancreatic ductal adenocarcinomas (PDACs). Serotonin (5-HT) is a neuromodulator with neurotransmitter and neuroendocrine functions that contributes to tumorigenesis. We investigated the role of 5-HT signaling in the growth of pancreatic tumors.</P> <P><B>Methods</B></P> <P>We measured the levels of proteins that regulate 5-HT synthesis, packaging, and degradation in pancreata from Kras<SUP>G12D/+</SUP>/Trp53<SUP>R172H/+</SUP>/Pdx1-Cre (KPC) mice, which develop pancreatic tumors, as well as in PDAC cell lines and a tissue microarray containing 81 human PDAC samples. We also analyzed expression levels of proteins involved in 5-HT synthesis and degradation by immunohistochemical analysis of a tissue microarray containing 311 PDAC specimens, and associated expression levels with patient survival times. 5-HT level in 14 matched PDAC tumor and non-tumor tissues were analyzed by ELISA. PDAC cell lines were incubated with 5-HT and cell survival and apoptosis were measured. We analyzed expression of the 5-HT receptor HTR2B in PDAC cells and effects of receptor agonists and antagonists, as well as HTR2B knockdown with small hairpin RNAs. We determined the effects of 5-HT stimulation on gene expression profiles of BxPC-3 cells. Regulation of glycolysis by 5-HT signaling via HTR2B was assessed by immunofluorescence and immunoprecipitation analyses, as well as by determination of the extracellular acid ratio, glucose consumption, and lactate production. Primary PDACs, with or without exposure to SB204741 (a selective antagonist of HTR2B), were grown as xenograft tumors in mice, and SB204741 was administered to tumor-bearing KPC mice; tumor growth and metabolism were measured by imaging analyses.</P> <P><B>Results</B></P> <P>In immunohistochemical analysis of a tissue microarray of PDAC specimens, increased levels of TPH1 and decreased level of MAOA, which regulate 5-HT synthesis and degradation, correlated with stage and size of PDACs and shorter patient survival time. We found levels of 5-HT to be increased in human PDAC tissues compared with non-tumor pancreatic tissues, and PDAC cell lines compared with non-transformed pancreatic cells. Incubation of PDAC cell lines with 5-HT increased proliferation and prevented apoptosis. Agonists of HTR2B, but not other 5-HT receptors, promoted proliferation and prevented apoptosis of PDAC cells. Knockdown of HTR2B in PDAC cells, or incubation of cells with HTR2B inhibitors, reduced their growth as xenograft tumors in mice. We observed a correlation between 5-HT and glycolytic flux in PDAC cells; levels of metabolic enzymes involved in glycolysis, the phosphate pentose pathway, and hexosamine biosynthesis pathway increased significantly in PDAC cells following 5-HT stimulation. 5-HT stimulation led to formation of the HTR2B–LYN–p85 complex, which increased PI3K–Akt–mTOR signaling and the Warburg effect by increasing protein levels of MYC and HIF1A. Administration of SB204741 to KPC mice slowed growth and metabolism of established pancreatic tumors and prolonged survival of the mice.</P> <P><B>Conclusions</B></P> <P>Human PDACs have increased levels of 5-HT, and PDAC cells increase expression of its receptor, HTR2B. These increases allow for tumor glycolysis under metabolic stress and promote growth of pancreatic tumors and PDAC xenograft tumors in mice.</P>

야구 및 체조 선수와 일반 남자 대학생들의 최대운동부하시 심폐기능의 비교 연구

朴炯俊(Park Heng Jun),朴喆斌(Park Chul Bin) 경희대학교 스포츠과학연구원 1994 체육학논문집 Vol.22 No.-

  The purpose of this study is under taken to difference of function that gymnastic, amatuer baseball, pro baseball and control group training of such team.<BR>  For the research the presenter of this study has 8 gymnastic player 8 A-baseball player 11 pro-baseball player 11 control each subject and abtain in function of respiro circulatory of the treadmill exercise test.<BR>  We drawn a conclusion as follosws.<BR>  1. Maximal heart rate was 187.9±4.9 beats/min in A-baseball group, 189.0±6.0 beats/min in Pro-baseball group, 192.3±7.2 beats/min in gymnastic group, 192.4±10.2 beats/min in control group.<BR>  2. Maximal Tidal Volume was 3.7±1.4 ℓ/f in pro-baseball 2.85±0.3 ℓ/f in A-baseball 2.6±0.4 ℓ/f in control group 2.2±0.2 ℓ/f in gymnastic group.<BR>  3. Maximal Ventilation was 146.1±49 ℓ/min in pro-baseball 132.75±7,8 ℓ/min A-baseball 114.2±12.6 ℓ/min in gymnastic 113.7±20.6 ℓ/min in control group.<BR>  4. Maximal respire respiratory was 1.4±0.2 in pro-baseball 1.18±0.1 in A-baseball group and gymnastic group 1.2±0.2 in control group.<BR>  5. Maximal oxygen uptake ?O₂ ㎖/㎏/min was 63.7±16.1 ㎖/㎏/min in pro-baseball 61.6±2.8 ㎖/㎏/min in A-baseball 57.5±5.9 ㎖/㎏/min in gymnastic 53.8±7.6 ㎖/㎏/min in control group.<BR>  6. Maximal oxygen uptake ?O₂ was 4.88±1.75 ℓ/min in pro-baseball 4.55±0.2 ℓ/min in A-baseball 3.53±0.23 ℓ/min in gymnastic 3.55±0.58 ℓ/min in control group.

Springback Nonlinearity of High-strength Titanium Alloy Tube upon Mandrel Bending

Heng Li,He Yang,Fei-Fei Song,Guang-Jun Li 한국정밀공학회 2013 International Journal of Precision Engineering and Vol. No.

Taking the stress-relieved Ti-3Al-2.5V tube as the objective, regarding both angular and radius springback, the nonlinear springback behaviors of the high-strength titanium tube (HSTT) upon the universal bending, viz., mandrel bending (rotary draw bending), are clarified. The experiments are conducted to identify the springback and validate the theoretical models, and the deformation theory of plasticity and the explicit/implicit 3D-FE based models are used to reveal the rules and the physical mechanism of the nonlinearity of elastic recovery. The results show that: 1) At early bending stages, the angular springback increases nonlinearly with larger bending angles, then it increases linearly when the bending angle exceeds a critical one; While the radius growth decreases exponentially with the increasing of the bending angles at early stages, then it remains unchanged when the bending angle exceeds another critical value; The springback behaves more nonlinearly under smaller bending radii; Both the critical values for the two springback phenomena become larger with smaller bending radii; The critical angle for radius springback is larger than the one for angular springback. 2) The relationship between the radius springback and angular one is further identified; The radius springback can be estimated by the angular springback of the bending regions of tube; The variation of bending radii has significant effect on angular and radius springback, while it has little influence on the springback angle by the straight regions; The variation of the material properties affects both the springback phenomena much more significantly than the changing of the processing parameters.

Transcriptome analysis to characterize the genes related to gonad growth and fatty acid metabolism in the sea urchin Strongylocentrotus intermedius

Heng Wang,Jun Ding,Siyu Ding,Yaqing Chang 한국유전학회 2019 Genes & Genomics Vol.41 No.12

Background Sea urchin gonads of both sexes, commonly termed “roe”, are highly valued seafood delicacies, and Strongylocentrotus intermedius is considered one of the tastiest sea urchins. In order to produce high-quality gonads for consumption and clarify the mechanism of gonad growth and development of the sea urchin, more genetic information, especially at the transcriptome level, is needed. Objective A more thorough understanding of sea urchin gonad growth and development in both sexes could enable regulation of these processes at several stages with the aim of suppressing gametogenesis in order to produce high-quality gonads for consumption. Methods The adult sea urchins S. intermedius were cultured for 3 months, and were sampled for the gonadal transcriptome analysis which has been performed on the RNAs of three male and female adults of S. intermedius in each gonad development stage. Results Illumina sequencing raw sequence data was deposited in the NCBI Sequence Read Archive (SRA) database (PRJNA532998). It generated 560,196,356 raw reads and 548,956,944 clean reads were acquired, which were assembled into 107,850 transcripts with 44,124 genes. Comparative analysis showed the differentially expressed genes (DEGs) from 114 to 2566. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were used to determine the functional significance of these DEGs. We have selected 9 genes related to growth and 12 genes related to fatty acid biosynthesis and metabolism in sea urchin gonads. Conclusion These data for sea urchins were intended to provide markers for gonad growth and development that can be accumulated for use in aquaculture applications.

Temperature characteristic of permittivity and splay elastic constant for fluorinated liquid crystal mixtures

Heng Ma,Jun Xu,Hiroyuki Okada,Hiroyoshi Onnagawa,Sigeru Sugimori,Kazuhisa Toriyama 한국물리학회 2013 Current Applied Physics Vol.13 No.5

This paper describes an experimental investigation for physical properties of fluorinated phenyl bicyclohexane liquid crystals (LCs) mixtures. Measurements are reported of the large anisotropic dielectric properties of two structurally similar LCs (1,2-difluoro-4-[trans-4-(trans-4-propylcyclohexyl)cyclohexyl]benzene and 1-cyano-2-fluoro-4-[trans-4-(trans-4-propylcyclohexyl) cyclohexyl] benzene) mixed with a zero dielectric anisotropic LC (1-methyl-2-fluoro-4-[trans-4-(trans-4-ethylcyclohexyl) cyclohexyl]benzene). The results indicate that the mixing proportions strongly influence the dielectric constants along with the longer molecular axis. For two samples which is made from meta-para- or meta-fluorine substituted molecules, the temperature dependence of the anisotropic dielectric striking resemble that of a unitary compound with para-fluorine substituted. The threshold voltage of the mixtures relies on the element that possesses a large dielectric anisotropic. The mixing of the materials leads to a slight increase for splay elastic constants in an anti-parallel measurement cell.