균류 분화과정의 유전적 조절기작

박범찬,이환희,박윤희,박희문 충남대학교 생물공학연구소 2003 생물공학연구지 Vol.9 No.1

Fungi are well suited for studying mechanism controlling development and cell differentiation in multicellular eukaryotes. The asexual and sexual reproductive cycles are tightly coordinated during the life cycle of Aspergillus nidulans. The asexual (mitotic) reproductive cycle involves the formation of a number of differentiated cell types. Successful formation of multicellular reproductive structures and spores (conidia) requires the establishment of spatiotemporal gradients in essential transcriptional regulators, such as brlA, abaA and wetA. Altered gene expression is coordinated with critical changes in the movement of nuclei and the regulation of the cell division cycle. Therefore, it has been proposed to define a central regulatory pathway. Several early regulatory genes involved in signal transduction pathway controlling asexual sporulation are also required for activating the central pathway. Sexual (meiotic) reproduction is more complex, requiring the differentiation of several specialized tissue types. Although technical difficulties have limited efforts to study the genetics of sexual fruiting-body formation directly, some recent progress has been made. With the recent availability of mutants blocked in sexual development, understanding the genetic interactions between genes that function primarily in determining sexual sporulation will be achieved in the near future.

Transmission ability of Zika virus with artificially infected Aedes albopictus in Korea

Yang Sung‐Chan,Lee Hee-Il,Kim Hyunwoo,Lee Wook‐Gyo 한국곤충학회 2021 Entomological Research Vol.51 No.8

Zika virus (ZIKV), a virus mainly occurring in South America, is now globally distributed. Understanding the pathogen transmission cycle in a vector is exceptionally important in developing disease control strategies. In this study, we performed ZIKV microinjection in Aedes albopictus (Skuese), to estimate its vertical and horizontal transmission ability. The virus infection rate was confirmed by real-time qPCR in the F1 generation derived from the ZIKV-injected Ae. albopictus. The minimum infection rate (MIR) of the F1 generation was highest when they were injected with the virus at 6–12 h (MIR: 16.1) and lowest at 12–24 h (MIR: 4.7) after feeding. In the developmental stage of the F1 progeny, MIR values were 1.0, 1.3, and 6.7 in each egg, larval, and adult stage, respectively, but ZIKV was not detected in the pupa stage. Virus transmission ability was not significantly different between the collection areas (Tongyeong and Jeju). Ae. albopictus demonstrated a high venereal transmission rate of ZIKV and was detected in males (6 pool/7 pool) and females (6 pool/7 pool), confirming that ZIKV can be transmitted from infected mosquitoes to uninfected mosquitoes via mating. This is the first study of the administration of a ZIKV microinjection to Ae. albopictus in Korea and suggests a possibility of a potential mechanism for the virus to survive during adverse conditions via vertical transmission.

프리미돈의 한국인에서의 생체이용율시험

권광일,윤민혁,윤희열,박희찬,권준택,심희옥,김동출 충남대학교 약학대학 의약품개발연구소 2004 藥學論文集 Vol.19 No.-

A reversed phase HPLC method was developed and validated for the determination of primidone in human plasma. Propylthiouracil was used as an internal standard. Calibration curves were linear in the concentration range of 10-120 ng/㎖. The coefficient of variation of the intra- and inter-day precision were below 15%. The coefficient of variation of the accuracy were below 15% in the concentration range investigated. A bioavailability study was performed using the validated HPLC method. Twenty four healthy human male volunteers were orally administered 20 mg of primidone. The pharmacokinetic parameters were calculated using WinNonlin. The mean values of AUC_(LAST) was 85.0±17.7 ㎍ hr/㎖, C_(max) was 4.22±1.04 ㎍/㎖, T_(max) was 1.85±1.13 hr, t_(1/2) was 17.7±2.4 hr. The pharmacokinetic parameters and the HPLC method can be used for the desigm of bioequivalence study of primidone.

특수 언어발달 장애 환아의 임상 및음향음성학적 특성연구

김연희,박세훈,신용일,김찬양,김현기,김정수 大韓再活醫學會 2000 Annals of Rehabilitation Medicine Vol.24 No.1

흰쥐 뇌줄기에 분포하는 Nitric Oxide Synthase 신경세포에 관한 면역조직화학적 연구

나준희,허영범,박찬,김정혜,유진화,안희경,이원규 대한해부학회 1999 Anatomy & Cell Biology Vol.32 No.2

전시를 위한 토기와 청자의 복원 : 토기기대, 노형기대, 청자사이호를 중심으로

강희숙,안병찬 국립중앙박물관 1999 박물관보존과학 Vol.1 No.-

국립김해박물관 개관전시 유물인 가야시대 土器 器臺 2점과 중앙박물관의 특별전 “고대국가의 형성” 전시회 출품 유물인 靑磁四耳壺에 대한 보존처리를 수행하였다. 육안 및 실체현미경 관찰을 통하여 처리 전 상태, 文樣과 製作技法 등을 파악하였고 이를 토대로 각 유물의 특성에 맞는 보존처리를 실시하였다. 기대는 결실부분이 커 에폭시系 복원재료를 이용해 파손된 토기 面과 직접 접착시켜 형태를 복원하고 문양과 색, 질감을 類似하게 처리하였다. 청자는 토기와 같은 복원재를 이용했지만 파손된 청자 면과 分離시켜 형태를 복원하여 해체가 가능하도록 조치한 다음 색과 질감도 구분이 되도록 처리하였다. Conservation treatment was done for the pottery-stand of Gaya period which was to be exhibited in celebration of the opening of Gimhae National Museum and for the jar of Chinese celadon which was to be exhibited in Korea National Museums special exhibition "Formation of Ancient States". Through the examinations by naked eyes and under the microscope, condition before treatment, patterns and manufacturing techniques of the objects were observed. According to these examinations, conservation treatments, suitable for each object was finished. As the pottery-stand was damaged severely, the epoxy resin was pasted directly on the broken surface of the pottery to restore the original shape and pattern, color and feeling similar to original state were given to the restored area. Although same restoration material was applied on the celadon, it was not pasted directly on the broken surface of the celadon so that the restored area could be dismantled, and color and feeling were also treated somewhat differently.

관상동맥 질환자를 위한 cycle ergometer exercise와 treadmill exercise의 적정 운동강도

유승희,장완성,박찬호,김승환,노호성 경희대학교 체육과학연구소 2000 體育學論文集 Vol.28 No.-

The purpose of this study was to determine heart rate (HR), ratings of perceived exertion (RPE) and oxygen uptake (Vo2) at the anaerobic threshold (AT) in two different exercise modalities, cycle ergometer and treadmil exercise (running). Subjects were 13 women patients with coronary heart disease. All sub-maximal and maximal Vo2 values but Vo2 at the AT for women were significantly higher in treadmill exercise than cycle ergometer. Submaximal and maximal HR values were not significantly different between the two exercise modalities. However, mean RPE values at the AT were significantly higher in cycle ergometer than treadmill exercise. We suggest that appropriate HR values in exercise training should be the same in cycling and treadmill exercise. while RPE values should be 1 or 2 higher in cycling than running.

제38회 국제법화학회 참석기

유영찬,박유신,정희선,이상기 內務部 國立科學搜査硏究所 2001 國立 科學 搜査 硏究所 年報 Vol.33 No.-

직장암환자에서 복회음병합절제술(Miles' operation) 후 발생한 지속적인 배뇨장애의 치료

박희석,김기찬,정호중 고신대학교 의학부 1998 高神大學校 醫學部 論文集 Vol.13 No.1-2

Bladder dysfunction is one of the serious postoperative complications after rectal cancer surgery. To get sufficient objective data and factors for adequate management of bladder dysfunction following rectal cancer surgery, we reviewed medical records of 122 patients who had abdominoperineal resection of rectum (Miles' operation) for rectal cancer. The incidence of bladder dysfunction in 122 rectal cancer patients was 18.9 percent (23 patients). Among 23 patients with bladder dysfunction, 10 patients were referred to our rehabilitation service and their bladder was areflexic type neurogenic bladder at referred time. In 10 patients referred to our rehabilitation service, 8 patients(80.0% got the balanced bladder and 2 patients(20.0% ) failed, and the interval between beginning of bladder training and establishment of balanced bladder in 8 patient was 13.4±6.8 days. In 13 patients did not referred to our service, only 3 patients(23.1% ) got the balanced bladder and 10 patients(76.9%) failed. To improve the life quality of the rectal cancer patients with neurogenic bladder following abdominoperineal resection of rectum, early proper rehabilitative managements including bladder training were should be given.

서울시 일부 대학생들의 건강습관과 건강상태의 관련성 연구

옥찬명,문인옥,김연희 대한보건협회 2001 대한보건연구 Vol.27 No.3

The purpose of this study was to find the association with the health practices and health status for university students. The subjects were taken 804 university students in seoul. The data were collected by self-administered questionnaire during 2 weeks from October 15, 2000. The findings were as follows: 1. Only 33.3% of the subjects had taken a health education-related class. 2. The male students showed better health practices than females on no snacks (p<0.001), going to bathroom regularly(p<0.001), taking a walk(p<0.01), and doing exercise(p<0.001). On the other hands the female students showed better practices such as no smoking and no drinking. 3. Religious showed higher health practice scores than non-religious ones(p<0.001). 4. Seventy five point nine percent of the respondents were in healthy group showing significant difference by sex (p<0.001) with 2.79 odds ratio. 5. The healthy group showed better health practices than non-healthy group such as on going to bathroom regularly(p<0.01), taking a walk (p<0.01), and having low stress(p<0.001). And the healthy group got more stress than the unhealthy group showing significant difference statistically (p<0.001). 6. To designate the factors influencing students' health status, logistic regression analysis has been done. According to the results of this analysis, health practices, and sex were selected as significant factors associated with the health status. The students who got higher health practices perceived their health better than lower health practices with odds ratio [OR] = 3.73 (p<0.001). And health practices had the most significant effect on health status. And the odds ratio of good health status was 2.61 for male students(p<0.001).