A Highly Pathogenic Strain of Bacillus thuringiensis serovar kurstaki in Lepidopteran Pests

Hatice Kati,Kazim Sezen,Remziye Nalcacioglu,Zihni Demirbag 한국미생물학회 2007 The journal of microbiology Vol.45 No.6

In order to detect and identify the most toxic Bacillus thuringiensis strains against pests, we isolated a B. thuringiensis strain (Bn1) from Balaninus nucum (Coleoptera: Curculionidae), the most damaging hazelnut pest. Bn1 was characterized via morphological, biochemical, and molecular techniques. The isolate was serotyped, and the results showed that Bn1 was the B. thuringiensis serovar, kurstaki (H3abc). The scanning electron microscopy indicated that Bn1 has crystals with cubic and bipyramidal shapes. The Polymerase Chain Reactions (PCRs) revealed the presence of the cry1 and cry2 genes. The presence of Cry1 and Cry2 proteins in the Bn1 isolate was confirmed via SDS-PAGE, at approximately 130 kDa and 65 kDa, respectively. The bioassays conducted to determine the insecticidal activity of the Bn1 isolate were conducted with four distinct insects, using spore-crystal mixtures. We noted that Bn1 has higher toxicity as compared with the standard B. thuringiensis subsp. kurstaki (HD-1). The highest observed mortality was 90% against Malacosoma neustria and Lymantria dispar larvae. Our results show that the B. thuringiensis isolate (Bn1) may prove valuable as a significant microbial control agent against lepidopteran pests.

Refractive Outcomes of Uneventful Cataract Surgery in Pseudoexfoliation Syndrome and Pseudoexfoliation Glaucoma

Hatice Tekcan,Mehmet Serhat Mangan,Serhat Imamoglu,Oksan Alpogan 대한안과학회 2022 Korean Journal of Ophthalmology Vol.36 No.3

Effect of the Reinforcement Phase on the Mechanical and Biocompatibility Properties of PLA Matrix Nano Composites

Hatice Evlen,Gulcin Erel 한국고분자학회 2021 폴리머 Vol.45 No.4

In this study, it was aimed to obtain nanocomposite structure with poly(lactic acid) (PLA) matrix and to improve the mechanical and morphological properties of the PLA matrix nano-reinforced composites. Titanium dioxide (TiO₂) and hydroxyapatite (HA) nanoparticles were used as reinforcing elements. Solvent casting particle leaching method was used for the production of the composites. Thermal characterization and compression test of the composite samples were carried out. Microstructural properties and the phases formed on the surface of in vitro tested samples were examined. As a result of the study, it was observed that nano-reinforced composites with PLA matrix are biocompatible and apatite formation increases gradually every week. Also the highest compressive strength was obtained in PLA/HA samples, while the lowest was obtained in PLA sample. It was concluded that composites prepared within the scope of the study can be used as artificial tissue scaffolds in biomedical and medical applications.

Endoscopic “Push-Trough” Technique Cartilage Myringoplasty in Anterior Tympanic Membrane Perforations

Hatice Celik,Erdal Samim,Derya Oztuna 대한이비인후과학회 2015 Clinical and Experimental Otorhinolaryngology Vol.8 No.3

Objectives. To evaluate endoscopic push-through technique cartilage myringoplasty results. Methods. This prospective study was performed on patients with anterior tympanic membrane perforations and endoscopic push-through technique cartilage myringoplasty was performed between 2011 and 2013. The patients who did not have any cholesteatoma or otorrhea in the previous 3 months, and had an air bone gap ≤25 dB in their preoperative audiograms were included in the study. They were followed up with endoscopic examination and audiograms at 2nd, 6th, 12th, and 24th postoperative months. Pure tone averages were calculated at 0.5, 1, 2, and 4 kHz frequencies. Results. Of 32 patients, 19 were females and 13 were males. The mean age was 40.3 years (range, 16 to 62 years), and the mean follow-up period was 12.4 months (range, 6 to 24 months). Graft success rate was 87.5% in this study. Preoperative mean air conduction hearing threshold was 25.9 dB, and the mean air-bone gap was 11.9 dB while these values improved to 19.5 dB and 5.3 dB respectively in the postoperative period. The mean hearing gain was 6.4 dB. The analysis of preoperative and postoperative mean air conduction thresholds and air bone gap values of the patients revealed statistically significant differences. Conclusion. Underlay cartilage myringoplasty with endoscopic push-through technique in anterior quadrant tympanic membrane perforations is an effective, minimally invasive and feasible method.

Effect of Premenstrual Syndrome on Work-Related Quality of Life in Turkish Nurses

Hatice Kahyaoglu Sut,Elcin Mestogullari 한국산업안전보건공단 산업안전보건연구원 2016 Safety and health at work Vol.7 No.1

Background: Little is known about the effects of premenstrual syndrome (PMS) on work-related quality of life in nurses. We aimed to investigate the effect of PMS on work-related quality of life in Turkish nurses. Methods: A total of 134 volunteer nurses were included in this cross-sectional study between January 2015 and March 2015. One hundred and thirty-four nurses completed a questionnaire regarding demographic data, the Premenstrual Syndrome Scale (PMSS), and the Work-Related Quality of Life Scale (WRQoL). The nurses were classified as having or not having premenstrual syndrome according to the PMSS. Results: The average age was 29.5±7.1 years and the prevalence of PMS was 38.1%. The total score of PMSS was significantly negatively correlated with the overall score (r = -0.341; p < 0.001) and all subscale scores of the WRQoL and ranged from -0.207 to -0.402 (p < 0.05 for all). All of the WRQoL subscale scores except stress at work (p = 0.179) in nurses with PMS were significantly lower than those of nurses without PMS (p < 0.05). The age (b = -0.258; p = 0.021) and PMSS total score (b = -0.314; p < 0.001) increment negatively; however, optimistic thinking (b = 0.228; p = 0.008) positively affected overall WRQoL score. Conclusion: Nurses with PMS have decreased levels of work-related quality of life in their professional lives. Methods to help cope with cyclic premenstrual symptoms may be used, and as a result, productivity and work-related quality of life may increase.

Formation, Characterization and Multifunctional Activity of Chitosan Nanoparticle Coating

Hatice Ibili,Mehmet Dasdemir 한국섬유공학회 2022 Fibers and polymers Vol.23 No.7

Multifunctional surface coating applications involve complicated multiple steps that need to be eliminated. In thispaper, single and coaxial electrospraying one-step methods are employed to generate nanoparticle coating that providesmultifunctional characteristics to covered surfaces in terms of antibacterial and superhydrophobic properties utilizingchitosan (CS) biopolymer with fluorochemical as water repellent agent (WRA). Material and process characteristics’ effecton nanoparticle spreading dispersity, particle dimension, morphology, and functionality are discussed. Relative humidityconditions are also taken as another variant to investigate its impact. Dynamic light scattering method, SEM images, andFESEM images are studied for nanomaterial and coating analyzes. It is found that electrosprayed high water concentrationsof solutions tend to show a bigger particle dimension. The increased flow rate of WRA is observed to be in favor of higherWCA values. In addition, elevated relative humidity amounts have an improving influence on the spreading of sprayedparticles, achieving higher water repellency and producing smaller particles. The results show that, for single electrospraying,untreated surfaces’ water repellency was dramatically improved and the mean water contact angle (WCA) increased from125.4 ° (±2.3 °) to 142.8 ° (±3.8 °) and reached 161 ° maximum level. While for coaxial electrospraying, mean WCA wasincreased to 153.8 ° (±3.8 °) and, reached 164.8 ° maximum WCA value after 10 min short application time. Up to 99.99 %,antibacterial activity was accomplished for Escherichia coli and Staphylococcus aureus bacteria.

Correlation between preoperative pressure pain assessments and anxiety and postoperative pain in impacted lower third molar surgery

Hatice Hosgor,Fatih Mehmet Coskunses,Berkay Tokuc 대한구강악안면외과학회 2021 대한구강악안면외과학회지 Vol.47 No.1

Objectives: The aim of this study was to evaluate correlations between anxiety and preoperative pressure pain assessments and postoperative pain and analgesic requirements in impacted lower third molar tooth surgery. Materials and Methods: This prospective study enrolled 60 patients who underwent impacted lower third molar surgery. The preoperative State- Trait Anxiety Inventory-I (STAI-I), pressure pain threshold, and pressure pain tolerance scores were measured. At 2, 4, 6, 12, and 24 hours, and at 6 days following surgery, the patients scored their pain on the visual analogue scale and recorded their analgesic drug usage. The data were evaluated, and the results were statistically analyzed. Results: Of the 60 patients, 38 were female. Mean age was 24.62±7.42 years. The study found no relationship between preoperative pressure pain assessments and postoperative pain (P>0.05). There was also no relationship observed between preoperative STAI-I scores and postoperative pain (P>0.05). However, there was a positive correlation between operation time and total medication taken (P<0.05). Conclusion: Preoperative pressure pain threshold, pressure pain tolerance, and anxiety level had no significant effects on postoperative pain and analgesic requirements in impacted lower third molar surgery.

Probabilistic sensitivity of base-isolated buildings to uncertainties

Hatice Gazi,Cenk Alhan 국제구조공학회 2018 Smart Structures and Systems, An International Jou Vol.22 No.4

Characteristic parameter values of seismic isolators deviate from their nominal design values due to uncertainties and/or errors in their material properties and element dimensions, etc. Deviations may increase over service life due to environmental effects and service conditions. For accurate evaluation of the seismic safety level, all such effects, which would result in deviations in the structural response, need to be taken into account. In this study, the sensitivity of the probability of failure of the structures equipped with nonlinear base isolation systems to the uncertainties in various isolation system characteristic parameters is investigated in terms of various isolation system and superstructure response parameters in the context of a realistic three-dimensional base-isolated building model via Monte Carlo Simulations. The inherent record-to-record variability nature of the earthquake ground motions is also taken into account by carrying out analyses for a large number of ground motion records which are classified as those with and without forward-directivity effects. Two levels of nominal isolation periods each with three different levels of uncertainty are considered. Comparative plots of cumulative distribution functions and related statistical evaluation presented here portray the potential extent of the deviation of the structural response parameters resulting from the uncertainties and the uncertainty levels considered, which is expected to be useful for practicing engineers in evaluating isolator test results for their projects.

Construction of genetic linkage map for Ficus carica L. based on AFLP, SSR, and SRAP markers

Hatice Ikten,Selcan Sevinc Solak,Osman Gulsen,Nedim Mutlu,Cengiz Ikten 한국원예학회 2019 Horticulture, Environment, and Biotechnology Vol.60 No.5

A new genetic linkage map of Ficus carica (2n = 2x = 26) was constructed using 149 F1 progeny derived from the crossbetween two fi g cultivars, ‘Bursa Siyahı’ (BS) and ‘Ak Ilek’ (AK). Fifty-two amplifi ed fragment length polymorphism, 49simple sequence repeat (SSR), 16 sequence-related amplifi ed polymorphism (SRAP), and 12 sequence characterized amplified region (SCAR–SRAP) combinations were used to generate markers for the map. The BS map consisted of 229 markers,distributed to 16 linkage groups (LGs), with an average marker density of 5.98 cM and map distance of 1342 cM. The AKlinkage map carried 244 markers, distributed to 16 LGs, with an average marker density of 4.90 cM and map distance of1191 cM. The consensus map comprises 355 markers, 1474 cM in length, with average marker density of 4.15 cM. The mapindicates the location of new SSRs, nine of which were transferred from related species, and might be helpful for mappingquantitative trait loci that control important horticultural traits in the future.

The Levels of Vitamin D, Vitamin D Receptor, Homocysteine and Complex B Vitamin in Children with Autism Spectrum Disorders

Hatice Altun,Ergül Belge Kurutaş,Nilfer Şahin,Olcay Güngör,Ebru Fındıklı 대한정신약물학회 2018 CLINICAL PSYCHOPHARMACOLOGY AND NEUROSCIENCE Vol.16 No.4

Objective: Autism spectrum disorder (ASD) is a complex neurodevelopmental syndrome with an increasingly prevalent etiology, yet not fully understood. It has been thought that vitamin D, complex B vitamin levels and homocysteine are associated with environmental factors and are important in ASD. The aim of this study was to examine serum vitamin D, vitamin D receptor (VDR), homocysteine, vitamin B6, vitamin B12 and folate levels in ASD. Methods: In this study, serum vitamin D and VDR, homocysteine, vitamins B6, B12 and folate levels were determined in 60 patients with ASD (aged 3 to 12 years) and in 45 age-gender matched healthy controls. In addition, calcium, phosphorus and alkaline phosphatase, which are associated with vitamin D metabolism, were measured from serum in both groups. ASD severity was evaluted by the Childhood Autism Rating Scale (CARS). Results: Serum vitamin D and VDR were substantially reduced in patients with ASD in comparision to control group. However, homocysteine level was significantly higher and vitamin B6, vitamin B12 and folate were also reduced in patients with ASD. Total CARS score showed a positive association with homocysteine and a negative correlation with vitamins D,B6, B12, folate and VDR. Conclusion: This comprehensive study, which examines many parameters has shown that low serum levels of vitamins D, B6, B12, folate and VDR as well as high homocysteine are important in the etiopathogenesis of ASD. However, further studies are required to define the precise mechanism(s) of these parameters and their contributions to the etiology and treatment of ASD.