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ISOLATION AND STRUCTURE DETERMINATION OF NEW MACROLIDE ANTIBIOTICS
HATFIELD, GEORGE M.,WOODARD, RONALD W.,SON, JONG-KEUN 영남대학교 약품개발연구소 1992 영남대학교 약품개발연구소 연구업적집 Vol.2 No.-
Three new bafilomycin-like compounds PD 118, 576-A1[1], PD 118, 576-A2 [2], and PD 118, 576-A3 [3] were isolated from a new soil Streptomyces species (WP 3913). The structures of PD 118, 576-A1, PD 118, 576-A2, and PD 118, 576-A3 were elucidated on the basis of spectroscopic studies including 2D nmr.
The Cancer Stem Cell Theory: Is It Correct?
유민혁,Dolph L. Hatfield 한국분자세포생물학회 2008 Molecules and cells Vol.26 No.5
The cancer stem cell hypothesis posits that tumor growth is driven by a rare subpopulation of cells, designated cancer stem cells (CSC). Studies supporting this theory are based in large part on xenotransplantation experiments wherein human cancer cells are grown in immunocompromised mice and only CSC, often constituting less than 1% of the malignancy, generate tumors. Herein, we show that all colonies derived from randomly chosen single cells in mouse lung and breast cancer cell lines form tumors following allografting histocompatible mice. Our study suggests that the majority of malignant cells rather than CSC can sustain tumors and that the cancer stem cell theory must be reevaluated.
CONTROLLED LYSIS OF ESCHERICHIA COLI DOUBLE - LYSOGEN OF BACTERIOPHAGES λHL1 AND φ434
Koo, Yoon Mo,Parekh, Bhavin S,Hatfield, G Wesley,Lim, Henry C 한국화학공학회 1996 Korean Journal of Chemical Engineering Vol.13 No.2
A novel phage double-lysogen was developed to produce an intracellular protein and disrupt the host cell in the same reactor. Using this double-lysogen, we could simplify the recovering processes without cell harvest and disruption. Construction of the double-lysogen is based on the fact that a lysogen of a phage can be superinfected by another phage with different immunity. The single-lysogen of Escherichia coli, P90c/λHL1, was superinfected with bacteriophage Φ434 to produce a double-lysogen, in which phage genomes from each phage coexisted in the host chromosome. Two different inducers were used to induice the double-lysogen to produce a protein and to lyse the host cell. The first phage genome, λHL1, the prophage of the original lysogen, containing the temperature sensitive cI_(857), lacZ and defective Q genes was induced by increasing temperature to produce β-galactosidase, an intracellular reporter protein. The overproduction of β-galactosidase was carried out without experiencing the cell lysis due to the defective Q gene. After the temperature shift, the second prophage from the lysogen MS21/Φ434 was induced by mitomycin C or ultra-violet light to lyse the cell. The lysis of the cell releases the intracellular protein to the outer space. The cell lysis was confirmed by the decrease of cell density and the increase of the extracellular activity of β-galactosidase at the same time.
Bohnsack, Alisha M.,Ibarra, Ilich A.,Hatfield, Peter W.,Yoon, Ji Woong,Hwang, Young Kyu,Chang, Jong-San,Humphrey, Simon M. Royal Society of Chemistry 2011 Chemical communications Vol.47 No.17
<P>The new porous phosphine coordination material, PCM-11, is an unusual 8,4-connected coordination polymer with an open 3-D pore structure, formed by reaction of Mg(<SMALL>II</SMALL>) with tris(<I>para</I>-carboxylato)triphenylphosphine oxide. The highly ionic nature of the metal–ligand bonding results in excellent thermal stability upon desolvation (>460 °C). PCM-11 is easily activated for small molecule sorption at low temperature without the requirement for solvent pre-exchange. It adsorbs 47.5 wt% CO<SUB>2</SUB> at 11.6 bar and 30 °C.</P> <P>Graphic Abstract</P><P>PCM-11: a highly stable Mg(<SMALL>II</SMALL>)-phosphine oxide coordination material that exhibits a very large CO<SUB>2</SUB> storage capacity at high pressure. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c1cc10754b'> </P>