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        • 조리 방법에 따른 고구마줄기의 Phytochemical 함량과 항산화 활성 비교

          양세희, 정혜진, 성예지, 이하나, 이세윤, 양진우, 이준수 忠北大學校 農業科學硏究所 2017 農業科學硏究 Vol.33 No.1

          Vegetable consumption is associated with health benefits. Recently, the interest in these food groups has grown among due to the identification of new compound in their chemical composition. Most of the vegetables are cooked by blanching, boiling, steaming, microwaving before consumed. These cooking processes may change not only food physical characteristics but also chemical composition. The present study was undertaken to investigate the effect of different cooking methods on phytochemical content and antioxidant capacity in Ipomoea batatas (L.) Lam stem. The result of study, Total polyphenol of phytochemical in Ipomoea batatas (L.) Lam stem was 6.88-12.65 mg GAE/100 g and flavonoid was 3.16-10.46 mg CE/100 g. Compared with the raw of Ipomoea batatas (L.) Lam stem, all cooking methods were found to significant correlations between the antioxidant activity assessed by ABTS, DPPH and polyphenol(p<0.05). These results can be provide the basic data how to prevent lose of nutrition in Ipomoea batatas (L.) Lam stem when cooking.

        • Integrated analysis of the transcriptomes and primary metabolite profiles of adventitious roots of P. ginseng cultivars

          Yun Sun Lee,Hyun-Seung Park,Dong-Kyu Lee,Murukarthick Jayakodi,Nam-Hoon Kim,Sang-Choon Lee,Jinkyung Kim,Hana Lee,Dong-Yup Lee,Sung Won Kwon,Tae-Jin Yang 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

          Panax ginseng C.A. meyer (family: Araliaceae) is a perennial crop that has been widely used as a traditional medicine in Korea. Various P. ginseng cultivars exhibit a range of morphological and physiological traits as well as genetic diversity. To elucidate the differences of primary metabolism underlying such genetic diverstiy, we performed primary metabolite profiles in adventitious roots from five Panax ginseng cultivars using gas chromatography-mass spectrometry (GC-MS). The GC-MS analysis revealed eight primary metabolites as biomarkers and allowed us to classify the five cultivars into three groups. We selected three cultivars to represent each group and analyzed their transcriptomes by Illumina sequencing. We inspected 100 unigenes involved in seven primary metabolite biosynthesis pathways and found that 21 unigenes encoding 15 enzymes were differentially expressed among the three cultivars. Integrated analysis of transcriptomes and metabolomes revealed that the ginseng cultivars differ in primary metabolites as well as in the putative genes involved in the complex process of primary metabolic pathways. Our data derived from this integrated analysis provide insights into the underlying complexity of genes and metabolites that co-regulate flux through these pathways in ginseng.

        • KCI등재

          Effect of Complexing/Buffering Agents on Morphological Properties of CuInSe<sub>2</sub> Layers Prepared by Single-Bath Electrodeposition

          Lee, Hana,Lee, Wonjoo,Seo, Kyungwon,Lee, Doh-Kwon,Kim, Honggon Korea Photovoltaic Society 2013 Current Photovoltaic Research Vol.1 No.1

          For preparing a device-quality $CuInSe_2$ (CISe) light-absorbing layer by single-bath electrodeposition for a superstrate-type CISe cell, morphological properties of the CISe layers were investigated by varying concentrations of sulfamic acid and potassium biphthalate, complexing/buffering agents. CISe films were grown on an $In_2Se_3$ film by applying a constant voltage of -0.5V versus Ag/AgCl for 90 min in a solution with precursors of $CuCl_2$, $InCl_3$, and $SeO_2$, and a KCl electrolyte. A dense and smooth layer of CISe could be obtained with a solution containing both sulfamic acid and potassium biphthalate in a narrow concentration range of combination. A CISe layer prepared on the $In_2Se_3$ film with proper concentrations of complexing/buffering agents exhibited thickness of $1.6{\sim}1.8{\mu}m$ with few undesirable secondary phases. On the other hand, when the bath solution did not contain either sulfamic acid or potassium biphthalate, a CISe film appeared to contain undesirable flake-shape $Cu_{2-x}Se$ phases or sparse pores in the upper part of film.

        • KCI등재

          Effect of Complexing/Buffering Agents on Morphological Properties of CuInSe<SUB>2</SUB> Layers Prepared by Single-Bath Electrodeposition

          Hana Lee,Wonjoo Lee,Kyungwon Seo,Doh-Kwon Lee,Honggon Kim 한국태양광발전학회 2013 Current Photovoltaic Research Vol.1 No.1

          For preparing a device-quality CuInSe2 (CISe) light-absorbing layer by single-bath electrodeposition for a superstrate-type CISe cell, morphological properties of the CISe layers were investigated by varying concentrations of sulfamic acid and potassium biphthalate, complexing/buffering agents. CISe films were grown on an In2Se3 film by applying a constant voltage of -0.5V versus Ag/AgCl for 90 min in a solution with precursors of CuCl2, InCl3, and SeO2, and a KCl electrolyte. A dense and smooth layer of CISe could be obtained with a solution containing both sulfamic acid and potassium biphthalate in a narrow concentration range of combination. A CISe layer prepared on the In2Se3 film with proper concentrations of complexing/buffering agents exhibited thickness of 1.6~1.8 μm with few undesirable secondary phases. On the other hand, when the bath solution did not contain either sulfamic acid or potassium biphthalate, a CISe film appeared to contain undesirable flake?shape Cu2-xSe phases or sparse pores in the upper part of film.

        • KCI등재

          Comparison Between South and North Korean Terms, Related to Clothing and Textiles

          Lee, Hana,Choi, Jin O,Lee, Yoon-Jung,Lee, Yhe-Young The Korean Society of Costume 2015 International journal of costume and fashion Vol.15 No.2

          The purpose of this study was to examine the differences in terminologies used in South and North Korea, to describe objects or activities related to clothing and textiles, as a part of a bigger project that aims at developing an educational program in provision of reunification of the Koreas. In this study, a total of 176 North Korean terms that differ from South Korean terms were collected from various sources, including dictionaries that are developed to compare South-North Korean languages as well as texts such as magazines and news articles, about North Korean daily life. The terms were classified into sub-categories: materials for clothing, clothing management, construction and design, garment names, body parts, description of physical appearance or state of hygiene, and apparel industry. Many of the North Korean terms were derived from native expressions, rather than adopting foreign terms or terms in Chinese characters. Some North Korean terms did not have any corresponding words in South Korean terms or vice versa. We expect the terminology list to become a useful educational resource in establishing a clothing and textiles curriculum in preparation of reunification, by allowing the students to familiarize with the differences in the usage of terms.

        • KCI등재

          UVB로 손상이 유도된 인간 피부 섬유아세포(Hs68)에서 일부 식품소재 에탄올 추출물의 피부 보호 효과

          이하나(Hana Lee),이종민(Jong Min Lee),홍성문(Sung Moon Hong),김나영(Na Young Kim),손석준(Seok Jun Son),정영재(Young Jae Jeong),이현순(Hyun-Sun Lee),이준수(Junsoo Lee) 한국식품영양과학회 2018 한국식품영양과학회지 Vol.47 No.6

          UVB 조사는 MMPs를 활성화시킴으로써 콜라겐을 감소시키고 결과적으로 주름을 형성한다. 본 연구에서는 식품소재 8종을 선정하여 에탄올 추출물을 제조하고 광노화 억제 효능을 평가하였다. 세포독성과 UVB에 대한 보호 효과는 MTT assay를 이용하여 측정하였다. ROS 발생 정도는 fluorescent spectrophotometer로 측정하였으며 수용성 콜라겐과 MMPs의 생성량은 assay kit을 사용하여 측정하였다. Hs68 세포주를 이용하여 50 μg/mL의 농도로 세포독성을 확인하였으며 시료의 독성은 나타나지 않았다. 레드파프리카, 토마토, 호박, 쥐눈이콩(중국산), 밤껍질 에탄올 추출물은 UVB 자극에 대해 각각 88, 104, 98, 97, 88%의 보호효과를 보였다. 이 추출물들은 UVB 조사로 유도된 ROS의 생성량을 감소시켰으며 콜라겐 생성량을 증가시켰다. 또한, 추출물들은 UVB 조사에 의한 MMP-1과 MMP-3의 증가를 억제했다. 그중 밤껍질이 UVB로 유도된 MMPs의 생성을 가장 효과적으로 감소시켰다. 결론적으로 본 연구를 통해 밤껍질 추출물은 자외선에 의한 피부노화와 주름 생성을 효과적으로 개선할 수 있는 가능성을 가짐으로써 항노화 및 항주름 소재로 화장품에 활용될 수 있을 것으로 기대된다. Ultraviolet B (UVB) irradiation is a major cause of photoaging and leads to the formation of skin wrinkles by degrading collagen via activation of matrix metalloproteinases (MMPs). In this study, we investigated the protective effects of ethanol extracts from selected food materials against UVB-induced photoaging in human skin fibroblast (Hs68) cells. The protective effects of the samples on cell viability were examined by MTT assay under UVB (30 mJ/cm2)-treated and untreated conditions. Intracellular levels of reactive oxygen species (ROS) were determined by using a fluorescent spectrophotometer. The levels of MMPs and collagen were measured by assay kits. The extracts (50 μg/mL) did not show any cytotoxicity. Extracts from red paprika, tomato, pumpkin, chestnut shell, and Jinuni bean showed protective effect against UVB irradiation (88, 104, 98, 97, and 88%, respectively). These extracts reversed elevation of ROS production and prevented reduction of collagen levels. Moreover, the extracts inhibited elevation of MMP-1 and MMP-3 levels caused by exposure to UVB irradiation in Hs68 cells. Among the samples tested, chestnut shell was found to exhibit the strongest inhibitory effect against elevation of UVB-induced MMPs. Taken together, these data suggest that chestnut shell is the most promising food material for treatment of UVB-induced photoaging in cosmetic products.

        • SCIESCOPUSKCI등재

          Baumann Skin Type in the Korean Male Population

          ( Young Bin Lee ),( Sung Ku Ahn ),( Gun Young Ahn ),( Hana Bak ),( Seung Phil Hong ),( Eun Jung Go ),( Chang Ook Park ),( Sang Eun Lee ),( Weon Ju Lee ),( Hyun-chang Ko ),( Jee-bum Lee ),( Hyung Joo K 대한피부과학회 2019 Annals of Dermatology Vol.31 No.6

          Background: Research into the Baumann skin type (BST) has recently expanded, with growing interest in the development of an efficient and effective skin type classification system for better understanding of this skin condition. Objective: We aimed to identify male-specific skin type characteristics with investigation into the distribution of BST by age and region in the Korean male population and to determine the intrinsic and extrinsic factors related to skin type. Methods: A questionnaire was administered to collect information about age, region, working behavior, drinking behavior, smoking behavior, usual habit of sun protection, medical history, and the BST which consisted of four parameters; oily (O) or dry (D), sensitive (S) or resistant (R), pigmented (P) or non-pigmented (N), and wrinkled (W) or tight (T). Results: We surveyed 1,000 Korean males aged between 20 and 60 years who were divided equally by age and region. Of the total re- Background: Research into the Baumann skin type (BST) has recently expanded, with growing interest in the development of an efficient and effective skin type classification system for better understanding of this skin condition. Objective: We aimed to identify male-specific skin type characteristics with investigation into the distribution of BST by age and region in the Korean male population and to determine the intrinsic and extrinsic factors related to skin type. Methods: A questionnaire was administered to collect information about age, region, working behavior, drinking behavior, smoking behavior, usual habit of sun protection, medical history, and the BST which consisted of four parameters; oily (O) or dry (D), sensitive (S) or resistant (R), pigmented (P) or non-pigmented (N), and wrinkled (W) or tight (T). Results: We surveyed 1,000 Korean males aged between 20 and 60 years who were divided equally by age and region. Of the total respondents, OSNW type accounted for the largest percentage and ORPW type the lowest. In terms of Baumann parameters, O type was 53.5%, S type was 56.1%, N type was 84.4% and W type was 57.5%. Several behavioral factors were found to have various relationships with the skin type. Conclusion: The predominant skin type in the Korean male respondents was OSNW type, and the distribution of skin types with regards to age and region was reported to be distinct. Therefore, skin care should be customized based on detailed skin types considering the various environmental factors. (Ann Dermatol 31(6) 621∼630, 2019)

        • SCOPUSKCI등재

          오타모반의 임상 및 병리조직학적 검색 -대한피부과학회 피부병리 연구분과위원회 공동연구-

          천승현,이상훈,박하나,전수영,손임범,김학용,최응호,황상민,이승헌,김중기,서성준,이주홍,점현,김상원,이정덕,박영민,김형욱,고보경,김일환,김수남,이석종,김유찬,이미우,고재경,김광호,김상태,서기석,안성구 대한피부과학회 2004 大韓皮膚科學會誌 Vol.42 No.3

          Background: Ota's nevus, characterized by the presence of melanocytes in the dermis, has been familiar among dermatologists, but the etiology, the pathophysiology, the clinical and histological classification are not entirely clear. To understand and elucidate them, more clinical studies and researches are necessary. Object: The aim of this study was to document the clinical and histopathological features of Ota's nevus. Methods: We examined 299 patients with Ota's nevus who visited the dermatology clinic in Korea from February 1993 to August 2003. Among them, 188 patients were biopsied. All the specimens were stained with hematoxylin and eosin. We examined the age & sex distribution, age of onset, seasonal variation, associated skin diseases, aggravating factors and color. We clinically classified Ota's nevus into 4 types according to Tanino's classification and histologically into 5 types based on the pattern of pigmentation. Results: 1. The ratio of male and female was 1:3. 2. The peak age of onset was at birth (28.4%) and puberty (24.8%). 3. Seasonal variation was observed with distinct aggravation in the summer (60%). 4. The associated diseases were 9 cases of persistent mongolian spot, 6 cases of nevus flameus, 4 cases of blue nevus, 3 cases of vitiligo, 3 cases of mevus of Ito, 2 cases of atopic dermatitis, 2 cases of psoriasis, 2 cases of cafe au late macules and 2 cases of contact dermatitis. 5. The aggravating factors were sunlight (35.8%), emotional stress (21.0%), menstruation (12.6%), cold exposure (9.5%), pregnancy (9.5%), fatigue (9.5%) and chemical agents (2.1%). 6. The color of lesions were blue black (36.8%), brown (34.8%), dark brown (16.1%) and slate (11.0%). 7. All cases were classified according to Tanino's methods: type Ia (23.1%), type Ⅰb (20.1%), type Ⅱ (26.7%), type Ⅲ (9.4%), type Ⅳ (20.7%). 8. The histological subtypes of Ota's nevus were classified as: the superficial type (35.6%); the middermis type (5.9%), the superficial-middermis type (18.6%); the mid-lower dermis type (2.7%); the diffuse type was composed of the superficial dominant type (19.7%), the middermis dominant type (4.8%), the dispersed (true diffuse) type (12.2%) and the deep dominant type (0.5%). 9. In the relation between histological types and the color of the lesion: the superficial type had 31 cases of brown color, 15 cases of blue black color, 11 cases of dark brown color and 9 cases of slate color; the middermis type had 6 cases of blue black color and 2 cases of dark brown color, query number of cases of slate color; the superficial-middermis type had 12 cases of blue black color, 10 cases of dark brown color, 7 cases of brown color and 6 cases of slate color: the mid-lower dermis type had 3 cases of blue black color; for the diffuse type, the superficial dominant type had 13 cases of brown color, query number of cases of blue black color and 7 cases of dark brown color; the mid-dermis dominant type had 4 cases of brown color, query number of cases of dark brown color, the dispersed type had 14 cases of blue black color and 5 cases of brown color, the deep dominant type had 1 case of blue black color. Conclusion: The histological reclassification of Ota's nevus may be very useful in making a therapeutic prognosis of the disease. (Korean J Dermatol 2004;42(3):272~280)

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