Introduction and application of a composite insect trap for the National Ecosystem Survey of Korea

Han Yong‐Gu,Hong Eui jeong,Kim Do‐Sung,Kim Chulgoo,Cha Jin Yeol 한국곤충학회 2020 Entomological Research Vol.50 No.10

This study aimed to establish the best applicable method for the National Ecosystem Survey (NES) guidelines by (i) verifying the efficiency of an insect trap that combined the Malaise trap, flight intercept trap, and pitfall trap (MFP trap) as part of the improvement for the terrestrial insect field in the fifth NES, and (ii) comparing the result with the currently used Malaise trap and pitfall trap (MP traps). We found that the efficiency of attaining species diversity was much higher with the added flight intercept trap function than the current Malaise trap alone for flying insect collection. For collecting ground insects, the efficiency of attaining species diversity of the pitfall trap from the MFP trap was lower than that from the MP trap. Besides, the Malaise trap of the MP trap showed superior efficiency than that of the MFP trap in species diversity. The results suggested that modification, supplementation, and re‐testing should be performed by analyzing various factors such as the trap material, barrier size, capturing container inlet, and mesh size, with consideration of the design of the current Malaise trap. Therefore, in survey‐based research, which aims at identifying insects species diversity and inhabiting status—such as the NES, it is necessary to introduce the MFP trap with the flight intercept trap function added to the Malaise trap function, and for the pitfall trap function of the MFP trap, it can be operated as a separate trap from the MFP trap.

cDNA Cloning and Genomic Structure of Glyceraldehyde-3-Phosphate Dehydrogenase from the Entomopathogenic fungus, Paecilomyces tenuipes-Jocheon

Nam Sook Park,Han Seok Kang,Seon Ku Kim,Yong Gyun Kim,Byung Uuk Cho,Teak Soon Shin,Keun Ki Kim,Hyun Chul Park,Hong Joo Son,Hong Gu Lee,Sang Mong Lee 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.05

Fungi belonging to the Paecilomyces spp. have recently been used as food and herbal medicines in Korea and are greatly popular as commercially available powdered supplement or dried fruiting body. Despite this acceptance and its use, little is known of the genes related to its reactive agents. Presently, We have constructed an olig-d(T) primed directional cDNA library from the silkworm Dongchunghacho, an entomopathogenic fungus, of which species is belonging to Paecilomyces spp. based on the previous identification of ITS1 and ITS2 at the molecular level and collected from Jocheon Miryang, Korea. To isolate and screen genes in the fungus, 626 expressed sequence tags(ESTs) were generated by a partial sequencing from the cDNA library. cDNA encoding the glyceraldehyde-3-phosphate dehydrogenase(Pt-GAPDH) of Paecilomyces tenuipes- Jocheon was cloned from the above cDNA library. The complete cDNA sequence of Pt-GAPDH is comprised of 1,014bp encoding 338 amino acid residues. The deduced protein sequence of Pt-GAPDH showed higher homology with Beauberia bassiana-GAPDH(93% amino acid identity). Hydropathy analysis revealed that Pt-GAPDH protein is hydrophilic. The major three amino acids in its composition of amino acid residues were alanine(11.54%), valine(9.47%) and glycine(8.88%). The Pt-GAPDH gene of Paecilomyces tenuipes entomopathogenic fungus consisted of three exons and two introns coding for 338 amino acid residues, and the genomic DNA length of the gene spans 1302bp. The accession number of the gene in GenBank are GU997099 for Pt-GAPDH cDNA and GU997102 for Pt-GAPDH genomic DNA. More investigation works including gene expression, immunological analysis etc. will be carried continuously without hesitation after this presentation.

Molecular Cloning, Expression and Genomic Structure of Glyceraldehyde-3-Phosphate Dehydrogenase from the Entomopathogenic fungus, Paecilomyces tenuipes Jocheon-1

Nam Sook Park,Han Seok Kang,Seon Ku Kim,Yong Gyun Kim,Byung Uuk Cho,Teak Soon Shin,Keun Ki Kim,Hyun Chul Park,Hong Joo Son,Hong Gu Lee,Sang Mong Lee 한국응용곤충학회 2011 한국응용곤충학회 학술대회논문집 Vol.2011 No.05

Presently, We have constructed an olig-d(T) primed directional cDNA library from the silkworm Dongchunghacho, an entomopathogenic fungus, of which species is belonging to Paecilomyces tenuipes Jocheon-1. To isolate and screen genes in the fungus, 626 expressed sequence tags(ESTs) were generated by a partial sequencing from the cDNA library. Paecilomyces tenuipes Jocheon-1 cDNA encoding the glyceraldehyde-3-phosphate dehydrogenase(Pt-GAPDH) of Paecilomyces tenuipes Jocheon-1 was cloned from the above cDNA library. The complete cDNA sequence of Pt-GAPDHis comprised of 1,014bp encoding 338 amino acid residues. The deduced protein sequence of Pt-GAPDH showed higher homology with Beauberia bassiana-GAPDH(93% amino acid identity). Hydropathy analysis revealed that Pt-GAPDH protein is hydrophilic. The major three amino acids in its composition of amino acid residues were alanine(11.54%), valine(9.47%) and glycine(8.88%). The cDNA encoding Pt-GAPDH was expressed as a 37 kDa polypeptide in baculovirus-infected insect Sf9 cells. The Pt-GAPDH gene of Paecilomyces tenuipes entomopathogenic fungus consisted of three exons and two introns coding for 338 amino acid residues, and the genomic DNA length of the gene spans 1302bp. The accession number of the gene in GenBank are GU997099 for Pt-GAPDH cDNA and GU997102 for Pt-GAPDH genomic DNA.

고무질 보강 식품포장용 LDPE필름의 기계적 강도와 기체투과 선택성

한신,강용구,박찬영 전남대학교 촉매연구소 2000 觸媒硏究 論文集 Vol.21 No.-

To improve the mechanical strength of the functional LDPE packaging film that has deficiency in the strength, though the film is active in vitality and has selective permeability for specific gases such as ethylene and carbon dioxide, this work blends a rubbery polymer, ethylene propylene monomeric rubber(EPM), in the LDPE film, and examines the mechanical strength and the permeability to assess preserving the functionality of freshness keeping of product. Overcoming the defect of incomplete coupling of the ceramic powder to the LDPE film is to use a coupling agent KBM-503( γ -methacryloxy propyl trimethoxy silane) of Shinetsu company in Japan. The optimum blending ratio of the EPM was about 5 percent in weight. The blended film reserves the selective gas permeability in relatively equivalent capacity of the non-rubbery film. The 5 percent EPM blended film has stronger mechanical strength than that made without the EPM, The strength approaches to the pure LDPE film. In conclusion, this work presents the method of improving mechanical strength without any depression in its freshness keeping functionality of the conventional packaging film.

산화아연계 무기질과 솔잎추출물 첨가에 의한 농산물 포장 필름의 광 및 미생물 열화능 개선

한신,나현정,강용구,박찬영 전남대학교 촉매연구소 2001 觸媒硏究 論文集 Vol.22 No.-

To invent environmentally friendly materials for packaging of agricultural produce, morphological and mechanical properly changes after daylight exposure and soil deposit for LDPE films composed of different incorporation substances have been investigated. Silver coated ceramic, zinc oxide, fine particle of ferrofluid and pine needle's extract were the incorporated substances. The pine needle's film degrades fast in soil while the silver coated ceramic's film shows daylight decay more on the morphological view. The mechanical strength keeps almost same. But turndown of elongation is explicit on the pine needle's film and silver coated ceramic's film.

주물사업장에서 발생하는 호흡성분진의 농도 및 석영함유량에 관한 연구

박용선,노영만,김현욱,한진구,안연순,강성규,김정만 한국산업위생학회 2003 한국산업보건학회지 Vol.13 No.1

This study was performed to measure and evaluate the concentration of respirable dust and quartz content at molding and shakeout process in the foundry. The 129 samples in 30 foundries were collected using 10mm Dorr-Oliver nylon cyclone and analysed for quartz concent by Fourier Transform Infrared Spectroscopy. The average quartz concentrations in the respirable dust was 0.0273mg/㎡ and ranged from 0.0007 to 0.3757mg/㎥. The average quartz concentrations of shakeout process wsa significantly higher than that of molding in the foundry. The average of respirable dust in foundry 102129mg/㎡ and ranged from 0.1524 to 7.0428mg/㎥. But there is no significant difference between molding and shakeout processes. The average contents of quartz for OEL-MOL was 2025% and ranged from 0.05 to 32.79% The non-compliance rates for quartz were 22.96% in Korea-OEL and 34.01% in ACGIH-TLV and NIOSH-REL, and 35.03% in OSHA-PEL. The airbome dust in the foundry was effectively controlled when local exhaust ventilation and/or wetting system were operated. The result of this study therefore showed that the engineering controls and periodic environmental suveillance in foundry will be very important for preventing the health effects of workers exposed to quartz.

인스텝 슛팅 동작에 대한 운동학적 분석

이용구,김무영,이한혁,조현영 한국스포츠리서치 2003 한국 스포츠 리서치 Vol.20 No.1

The purpose of this study was to investigate the kinematic variables of the lower extremity during the Soccer In-step shooting motion. To conduct an investigation, five soccer players(skill group) and five students(less-skill group) in the high school were participate in this study. They were videotaped using three video cameras with the frame rate being 60㎐. This data was then reducted to 3D coordinates using the DLT(direction linear transformation)method. It was concluded that; Medio-lateral(X) displacement of the skill group was larger than that less-skill group and antero-posterior(Y) displacement of the skill group was smaller than that less-skill group. Velocity transfer of the skill group was more effectiveness than less skill group. The sequence of the maximal joint velocity of the kicking leg was the hip, knee, and ankl joint. It is considered very important that the sequence of the laximal joint velocity is from the proximal to the distal in order to increase the foot velocity at the impact. Trunk angle of the skill group was smaller than that less-skill group. And then ankle joint angle of the skill group was larger than that less-skill group.

주정중독 환자와 정상음주자에 대한 감정상태와 자기 지각, 가정환경, 사회적 지지에 관한 비교 연구

이정식,한광수,이유상,한일우,신용구 용인정신의학연구소 1997 용인정신의학보 Vol.4 No.1

기체-고체 유동상에서 고체입자의 혼합특성에 관한 실험적 연구

金鍾億,陰漢龍,金聖九 弘益大學校 科學技術硏究所 1997 科學技術硏究論文集 Vol.8 No.-

In the present study, mixing characteristics of solid particles in bubbling fluidized bed have been analyzed in a freely bubbling two-dimensional bed experimentally. Verticall and horizental particles mixing was studied by using heated particles as tracers. The steady-state temperature patterns around a heated wire and transient response to an injected pulse of heated particles were measured. A qualitative study of bubble size, velocity and void fraction in the two -dimensional bed was made by using a high-speed viedo recorder. Through the screen anlaysis of bubble flow it was verified that the size of bubbles increases with the rise of excesive velocity or bed height. Steady state experitments gave the result that the movement of heated particles in beds was more vigorous in higher excessive velocity. In trasient experimet the higher excesive velocity delay time of high temperature particles injected into the bed were the shorter. Dispersion coefficient was found to be increased with grouth of the bubble size and excessive velocity.

鷄龍山의 水棲昆蟲 群集에 관한 硏究

남상호,조영호,박영준,한용구,이정수 대전대학교 기초과학연구소 2002 自然科學 Vol.13 No.2

천황봉에서 발원하여 산간 계류를 형성하고 흐르다가 유성천과 합류해 금강으로 유입되는 계룡산 동학사 계곡의 수선곤충 군집을 조사한 결과 총 5목 17과 37종이 출현하였다. 이중에서는 하루살이류가 6과 14종으로 가장 많이 출현하였으며, 현존량은 파리류가 전체 1,165개체중 569개체(48.84%)로 가장 높게 나타났다. 전체 우점종은 꼬마하루살이와 깔다구류로 0.15의 우점도지수를 기록하였다. 종다양도는 제 4지점인 남매탑 계류의 합류점이 0.93으로 가장 높았고, 제 1지점인 은선산장 위 부근이 0.09로 낮게 나타났다. 각 지점별 오수생물계열은 초강부수성-강부수성으로 매우 낮게 조사되었다. Aquatic insect communities were investigated in a mountain streams of YIt. Iiyeryong1 one of the central areas of Korea, from March to September, 2002. As a result, 37 species of aquatic insects in 17 families and in 6 orders appeared during the study period, the order Ephemeroptera and Diptera of which were the most appeared. Ephemeroptera was consisted of 14 species of 6 families, and Diptera was consisted of 589 individuals(48.84%j of total individual nun-her. Dominant species was Baetis ther-micus and the subdominant species was Chironmidae sp.. Dominant species index of Baetis thermicils and Chironmidae sp. was O.15 The highest species diversity index was observed of site 4 as 0.93 and the lowest species diversity index was observed of site 1 as 0.09. Dominance indices ranged between 0.15 and 1.00 while diversity indices between 0.09 and 0.93. In relation to species diversity indices, Mt. Iiyeryong streams were determined as Isosaprobity-Polysaprobity area. In general, it is inferred from the aquatic insect community that typical mountain streams of Korea are low.