Reduction of Hydrogen Sulphide in Chicken Manure by Immobilized Sulphur Oxidising Bacteria Isolated from Hot Spring

M. Y. Hidayat1,H. M. Saud,A. A. Samsudin 한국미생물·생명공학회 2019 한국미생물·생명공학회지 Vol.47 No.1

The rapid development of the poultry industry has led to the production of large amounts of manure, which produce substances like hydrogen sulfide (H2S) that contribute to odor pollution. H2S is a highly undesirable gas component and its removal from the environment is therefore necessary. Sulfur-oxidizing bacteria (SOB) are widely known to remove contaminating H2S due to their ability to oxidize reduced sulfur compounds. In this study, three potential SOB (designated AH18, AH25, and AH28) that were previously isolated from a hot spring in Malaysia were identified by 16S rRNA gene analysis. Laboratory-scale biological deodorization experiments were conducted to test the performance of the three isolates—in the form of pure or mixed cultures, with the cells immobilized onto alginate as a carrier—in reducing the H2S from chicken manure. On the basis of 16S rRNA phylogenetic analysis, isolate AH18 was identified as Pseudomonas sp., whereas isolates AH25 and AH28 were identified as Achromobacter sp. The most active deodorizing isolate was AH18, with an H2S reduction rate of 74.7% (p < 0.05). Meanwhile, the reduction rates for isolates AH25 and AH28 were 54.2% and 60.8% (p > 0.05), respectively. However, the H2S removal performance was enhanced in the mixed culture, with a reduction rate of 81.9% (p < 0.05). In conclusion, the three potential SOB isolates were capable of reducing the H2S from chicken manure in the form of a pure culture immobilized on alginate, and the reduction performance was enhanced in the mixed culture.

Numerical desirability function for adsorption of methylene blue dye by sulfonated pomegranate peel biochar: Modeling, kinetic, isotherm, thermodynamic, and mechanism study

Ali H. Jawad,Ahmed Saud Abdulhameed,M. A. K. M. Hanafiah,Zeid A. AlOthman,Mohammad Rizwan Khan,S. N. Surip 한국화학공학회 2021 Korean Journal of Chemical Engineering Vol.38 No.7

Sulfonated pomegranate (Punica granatum) peel biochar (SPPBC) was developed via thermal activation with sulfuric acid (H2SO4) to act as a promising biochar material for the adsorptive removal of toxic cationic dye namely methylene blue (MB) dye from contaminated water. A Box-Behnken design (BBD) and numerical desirability function were adopted to optimize the input adsorption variables (SPPBC dosage, temperature, pH, and contact time). The maximum removal of the MB dye can be accomplished by simultaneous significant interaction between SPPBC dosage with solution pH, SPPBC dosage with time, SPPBC dosage with temperature, solution pH with time, and time with temperature. The numerical desirability function identified the highest MB dye removal (93.9%) can be achieved at the following optimum numerical adsorption conditions: SPPBC dosage 0.18 g, temperature 49 oC, pH 9.7, and time 4.3 h. Equilibrium data were well fitted to the Temkin and Langmuir isotherm models. The maximum recorded adsorption capacity of SPPBC for MB dye adsorption by using Langmuir isotherm model was 161.9mg/g. This research work reveals the possibility of converting lignocellulose pomegranate peel into a renewable and environmentfriendly biochar via a relatively fast acid-activation process with the great potential to be promising adsorbent for removal of MB dye.

Exogenous Application of Nitric Oxide and Spermidine Reduces the Negative Effects of Salt Stress on Tomato

Manzer H. Siddiqui,Saud A. Alamri,Mutahhar Y. Al-Khaishany,Mohammed A. Al-Qutami,Hayssam M. Ali,Hala AL-Rabiah,Hazem M. Kalaji 한국원예학회 2017 Horticulture, Environment, and Biotechnology Vol.58 No.6

Due to increasing soil salinity, the world agricultural output is being threatened by the shrinking area offertile land. In the present study, we explored the interactive roles of nitric oxide (NO; 100 μM) and spermidine (SP;200 μM) in ameliorating the effects of salt stress (NaCl; 100 mM) in tomato (Solanum lycopersicum L. var. FiveStar) seedlings. NaCl stress reduced shoot and root length, shoot and root fresh weight, shoot and root dry weightplant-1 and leaf area leaf-1. NaCl stress also suppressed the biosynthesis of photosynthetic pigments (Chlorophyll aand b) and increased proline (Pro) content, membrane damage and lipid peroxidation by inducing reactive oxygenspecies (H2O2 and O2•−) generation in roots and leaves, as well as electrolyte leakage (EL) and malondialdehyde(MDA) accumulation in leaves. However, applying NO and/or SP increased the activities of catalase, peroxidase,superoxide dismutase, glutathione reductase and ascorbate peroxidase and increased photosynthetic pigment (chlorophylla and b) and Pro accumulation, as well as reducing H2O2, O2•− and MDA content and EL, under salt stress. Whentomato plants were treated with NO and SP simultaneously, NO signaling was further enhanced, which was confirmedby the addition of cPTIO [2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide; NO scavenger].

Supercapacitors based on ternary nanocomposite of TiO2&Pt@graphenes

Ghouri, Z. K.,Barakat, N. A.,Saud, P. S.,Park, M.,Kim, B. S.,Kim, H. Y. Springer Science + Business Media 2016 Journal of materials science Materials in electron Vol.27 No.4

<P>The electrochemical properties of graphene can be significantly enhanced due to the incorporating of hetero-atoms into the graphene. In this article Crumpled-like TiO2-Pt/graphene nanocomposite from graphene oxide (GO), titanium (III) chloride (TiCl3) and Hydrogen hexa chloro palatinate (H2PtCl6) was synthesized via a simple reflux strategy and employed as a simple capacitor electrode material. The nanocomposite was characterized by XRD, XPS, FESEM, HRTEM and the electrochemical properties were investigated by cyclic voltammetry (CV). Electrochemical characterization for introduced nanocomposite indicated that the corresponding specific capacitance is 160 F/g (at 5 mV/s) with good stability. The high electrochemical performance is recognized to the presence of Pt nanoparticles on the nanocomposite and graphene distinct characteristics. To the best of our knowledge this is the first report on TiO2-Pt/graphene nanocomposite as an electrical double layer capacitor material.</P>

Reduction of Hydrogen Sulphide in Chicken Manure by Immobilized Sulphur Oxidising Bacteria Isolated from Hot Spring

Hidayat, M.Y.,Saud, H.M.,Samsudin, A.A. The Korean Society for Microbiology and Biotechnol 2019 한국미생물·생명공학회지 Vol.47 No.1

The rapid development of the poultry industry has led to the production of large amounts of manure, which produce substances like hydrogen sulfide ($H_2S$) that contribute to odor pollution. $H_2S$ is a highly undesirable gas component and its removal from the environment is therefore necessary. Sulfur-oxidizing bacteria (SOB) are widely known to remove contaminating $H_2S$ due to their ability to oxidize reduced sulfur compounds. In this study, three potential SOB (designated AH18, AH25, and AH28) that were previously isolated from a hot spring in Malaysia were identified by 16S rRNA gene analysis. Laboratory-scale biological deodorization experiments were conducted to test the performance of the three isolates-in the form of pure or mixed cultures, with the cells immobilized onto alginate as a carrier-in reducing the $H_2S$ from chicken manure. On the basis of 16S rRNA phylogenetic analysis, isolate AH18 was identified as Pseudomonas sp., whereas isolates AH25 and AH28 were identified as Achromobacter sp. The most active deodorizing isolate was AH18, with an $H_2S$ reduction rate of 74.7% (p < 0.05). Meanwhile, the reduction rates for isolates AH25 and AH28 were 54.2% and 60.8% (p > 0.05), respectively. However, the $H_2S$ removal performance was enhanced in the mixed culture, with a reduction rate of 81.9% (p < 0.05). In conclusion, the three potential SOB isolates were capable of reducing the $H_2S$ from chicken manure in the form of a pure culture immobilized on alginate, and the reduction performance was enhanced in the mixed culture.

Photoluminescent and transparent Nylon-6 nanofiber mat composited by CdSe@ZnS quantum dots and poly (methyl methacrylate)

Alam, A.M.,Ghouri, Z.K.,Barakat, N.A.M.,Saud, P.S.,Park, M.,Kim, H.Y. IPC Science and Technology Press 2016 Polymer Vol.85 No.-

In this work, a photoluminescent (PL) and transparent nanocomposite mat was demonstrated based on Nylon-6 (PA-6) nanofibers (NFs) mat, core-shell CdSe@ZnS quantum dots (QD) and Polymethyl methacrylate (PMMA) polymeric solution. Green and red emissive CdSe@ZnS QD was synthesized by one step approach, involving chemical composition gradient technique using standard schlenk line. Dip coating method was adopted to decorate PA-6 NFs mat with CdSe@ZnS QD and PMMA thin layer in sequence to introduce PL properties and optical transparency, respectively in the nanocomposite mat. The morphology of NFs, existence of QD in the nanocomposite, and optical characteristics such as PL and visible light transmittance of the nanocomposite were examined by various physio-chemical techniques. The dried PA-6/CdSe@ZnS/PMMA nanocomposite mats exhibited light transparency in daylight condition as well as emission of green and red color similar to the individual PL of green and red emitting CdSe@ZnS QD when they were excited under UV light or in a spectrofluorimeter. The results indicated the well preserved quantum confinement properties of the prepared QD in the mat. The fabricated mat found to be demonstrated transparency over 50% in the visible wavelength range of 400-800 nm.

Synthesis and characterization of photocatalytic and antibacterial PAN/Ag2CO3 composite nanofibers by ion exchange method

Saud, P. S.,Pant, B.,Ghouri, Z. K.,Panthi, G.,Park, S. J.,Han, W.,Park, M.,Kim, H. Y. THE KOREAN FIBER SOCIETY 2015 Fibers and polymers Vol.16 No.6

<P>Highly photocatalytic and antibacterial Ag2CO3 nanoparticles were incorporated into PAN nanofibers by electrospinning technique followed by ion exchange reaction between silver nitrate and sodium bicarbonate at room temperature. The samples were characterized by field-emission scanning electron microscopy (FE-SEM), Transmission electron microscopy (TEM), X-ray diffraction (XRD), photoluminescence (PL), and Fourier transformed infrared (FT-IR) spectroscopy. The as prepared sample was found to exhibit an excellent photocatalytic activity toward degradation of methylene blue (MB) in aqueous solution and antibacterial properties against Escherichia coli colt) under visible light. The perfect recovery of catalyst after reaction and its unchanged efficiency for cyclic use showed that it will be an economically and environmentally friendly photocatalyst for the water purification.</P>

Teleworking Survey in Saudi Arabia: Reliability and Validity of Arabic Version of the Questionnaire

Heba Yaagoub AlNujaidi,Mehwish Hussain,Sama’a H. AlMubarak,Asma Saud AlFayez,Demah Mansour AlSalman,Atheer Khalid AlSaif,Mona M. Al-Juwair 대한예방의학회 2022 예방의학회지 Vol.55 No.6

Objectives: This study aimed to adapt the survey questionnaire designed by Moens et al. (2021) and determine the validity and reliability of the Arabic version of the survey in a sample of the Saudi population experiencing teleworking. Methods: The questionnaire includes 2 sections. The first consists of 13 items measuring the impact of extended telework during the coronavirus disease 2019 (COVID-19) crisis. The second section includes 6 items measuring the impact of the COVID-19 crisis on self-view of telework and digital meetings. The survey instrument was translated based on the guidelines for the cultural adaptation of self-administrated measures. Results: The reliability of the questionnaire responses was measured by Cronbach’s alpha. The construct validity was checked through exploratory factor analysis followed by confirmatory factor analysis (CFA) to further assess the factor structure. CFA revealed that the model had excellent fit (root mean square error of approximation, 0.00; comparative fit index, 1.0; Tucker-Lewis index, 1; standardized root mean squared residual, 0.0). Conclusions: The Arabic version of the teleworking questionnaire had high reliability and good validity in assessing experiences and perceptions toward teleworking. While the validated survey examined perceptions and experiences during COVID-19, its use can be extended to capture experiences and perceptions during different crises.