녹차, 오룡차 및 홍차 추출물의 돌연변이원성 억제작용

여생규(Saeng-Gyu Yeo),김인수(In-Soo Kim),안철우(Cheol-Woo Ahn),김선봉(Seon-Bong Kim),박영호(Yeung-Ho Park) 한국식품영양과학회 1995 한국식품영양과학회지 Vol.24 No.1

차추출물의 돌연변이원성 억제작용은 아주 우수한 것으로 나타났으며, 이 중 조catechin 획분에서 가장 높았다. 또한 농도가 증가할수록 억제작용도 증가하는 것으로 나타났다. 차 종류 중 발효차인 홍차의 수용성 획분에서 음용조건인 0.5~1.0㎎/plate의 농도에서 PhlP에 대하여 70.9~91.0%, MelQx에 대하여는 92.2~98.8%로 돌연변이원성 억제작용이 높게 나타났다. 돌연변이원성 억제작용에 관여하는 주된 인자는 차 polyphenol 화합불인 catechin류 중 PhlP에 대하여 주로(-)-EGC 및 (-)-EGCg와 MelQx에 대하여는 (-)-EC와 (-)-EGC이 관여하며, 또한 갈변물질도 관여하는 것으로 나타났다. 돌연변이원성 억제작용 기구는 시료 자체가 직접적으로 변이원 물질을 불활성화시킴으로써 돌연변이원성 억제작용을 나타내는 것으로 밝혀졌다. Desmutagenicities against 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhlP) and 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MelQx) of tea extracts (steamed green tea, roasted green tea, oolong tea and black tea) were investigated. All the fractions obtained from tea extracts showed strong desmutagenic activity against PhlP and MelQx toward S. typhimurium TA 98 in the presence of the S-9 mix. The crude catechin fraction exhibited the strongest desmutagenic activity. Among these tea extracts, black tea especially exhibited the strongest desmutagenic activity and the activity was 70.9~91.0% against PhlP and 92.2~98.8% against MelQx at a concentration (0.5~1.0㎎/plate) for drinking. The activity of authentic catechins of (-)-EGC, (-)-EGCg, (-)-ECg and (-)-EC were 79.5%, 60.2%, 46.1% and 43.5% against PhlP, and were 52.3%, 11.6%, 8.2% and 22.1% against MelQx by addition of 1.0㎎/plate, respectively. The desmutagenic activity was supposedly due to the (-)-EGCg, (-)-EGC and (-)-EC in tea polyphenols, and the browning materials. The desmutagenicity was stronger when mutagens were preincubated with S-9 mix after reaction with black tea extracts than when preincubated with them after reaction with S-9 mix. The desmutagenicity of tea extracts was rather expressed by reacting directly with mutagens than by deactivating the activated forms of mutagens.

녹차, 오룡차 및 홍차 추출물의 Xanthine Oxidase 억제작용

여생규(Saeng-Gyu Yeo),박영범(Yeung-Beom Park),김인수(In-Soo Kim),김선봉(Seon-Bong Kim),박영호(Yeung-Ho Park) 한국식품영양과학회 1995 한국식품영양과학회지 Vol.24 No.1

Xanthine oxidase 저해작용은 차 polyphenol 화합물인 조catechin 획분에서 가장 뛰어났으며, 차 종류 별로는 비발효차인 녹차 중 증제차에서 93.2%로 저해활성이 가장 높게 나타났다. 그리고 차추출물의 농도가 증가할수록 저해효과는 높았으며, 이들 차추출물을 1, 2 및 3차에 걸쳐 추출하여 추출횟수에 따른 저해작용은 서로 뚜렷한 차이는 없였다. 또한 차 종류 중 발효가 진행된 차일수록 저해작용은 떨어지는 것으로 나타났다. 차추출물의 catechin류 함량을 살펴 본 결과, 증제 차, 몪음차 및 오룡차에서는 (-)-EGCg>(-)-EGC>(-)ECg>(-)-EC의 순으로 나타났으며, 흥차에서는 (-)-ECg>(-)-EGC>(-)-EC>(-)-EGCg의 순으로 나타났는데, 차 제조시 발효가 진행된 차일수록 전반적인 catechin류 함량이 낮게 나타났다. 이들 차추출물의 xanthine oxidase 저해작용에 관여하는 인자를 검색한 결과, 차 polyphenol 화합물인 catechin류의 분자구조 중 gallate기가 결합되어 있는 (-)-EGCg 및 (-)-ECg인 것으로 추정되었다. Inhibition of xanthine oxidase by tea extracts obtained from non-fermented tea (steamed green tea and roasted green tea), semi-fermented tea (oolong tea) and fermented tea (black tea) were investigated. The crude catechin fraction had a higher inhibitory effect against xanthine oxidase, and the effect was increased with the addition of tea extracts. Their inhibitory effect were hardly influenced until extracted three times with hot water. According to the investigation of catechins in the crude catechin fraction obtained from tea extracts, (-)-epicatechin-(EC), (-)-epicatechin gallate (ECg), (-)-epigallocatechin(EGC) and (-)-epigallocatechin gallate (EGCg) were 80.1㎍/㎎, 113.5㎍/㎎, 186.3㎍/㎎ and 367.7㎍/㎎ in steamed green tea, and 75.6,㎍/㎎, 114.7,㎍/㎎, 193.7㎍/㎎ and 381.9㎍/㎎ in roasted green tea, and 69.4,㎍/㎎, 110.0㎍/㎎, 127.1㎍/㎎ and 464.9㎍/㎎ in oolong tea, and 78.1㎍/㎎, 171.8㎍/㎎, 80.7㎍/㎎ and 51.4㎍/㎎ in black tea, respectively. Order of the content of these catechins was (-)-EGCg>(-)-EGC>(-)-ECg>(-)-EC in steamed green tea, roasted green tea and oolong tea, and was (-)-ECg>(-)-EGC>(-)-EC>(-)-EGCg in black tea. Also the concentration of catechins was hardly influenced until extracted three times. The inhibition ratio of xanthine oxidase by authentic catechins was 94.9% and 87.6% by addition of 5.0㎍/ml of (-)-EGCg and (-)-ECg, respectively. The inhibitors of xanthine oxidase were supposed to be due to (-)-ECg and (-)-EGCg in tea polyphenol compounds.

참치 자숙액의 Angiotensin 전환효소 저해작용

여생규(Saeng Gyu Yeo),이태기(Tae Gee Lee),안철우(Cheol Woo Ahn),김인수(In Soo Kim),구연숙(Yeun Suk Gu),박영호(Yeung Ho Park),김선봉(Seon Bong Kim) 경상대학교 해양산업연구소 1998 해양산업연구소보 Vol.10 No.-

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녹차, 오룡차 및 홍차 추출물의 항산화효과

여생규(Saeng-Gyu Yeo),안철우(Cheol-Woo Ahn),이용우(Yong-Woo Lee),이태기(Tae-Gee Lee),박영호(Yeung-Ho Park),김선봉(Seon-Bong Kim) 한국식품영양과학회 1995 한국식품영양과학회지 Vol.24 No.2

차 추출물의 항산화작용은 모두 높은 것으로 나타났으며, 시료의 양이 증가할수록 항산화작용도 증가하는 것으로 나타났다. 각 획분 중 차 polyphenol 화합물인 조 catechin 획분에서 가장 뛰어났으며, 차 종류 중 반발효차인 오룡차가 가장 높은 항산화작용을 나타내어 0.5㎎의 양에서 37℃, 10일간 저장하였을 때 32meq/㎏의 과산화물값을 나타내었다. 또한 차 추출물 모두 free radical 소거작용이 있는 것으로 나타났으며, 이중 비발효차인 녹차 및 반발효차인 오룡차가 가장 우수한 것으로 나타났고, 차 종류 중 가장 항산화작용이 뛰어난 오룡차는 BHT 및 α-tocopherol과의 상승효과가 있었으며, 이 중 α-tocopherol 보다 BHT에 대해 더 높은 상승효과를 나타내었다. 그리고 Cu^(2+) 보다 Fe^(3+) 이온에 대하여 금속이온 봉쇄작용이 강한 것으로 나타났다. 따라서 차 추출물의 항산화작용은 활성산소에 의한 지질의 과산화반응을 억제하고, 또한 금속이온에 대한 봉쇄작용도 아울러 가지고 있는 것으로 생각되며, 그 주된 인자는 차 polyphenol 화합물인 catechin류가 관여하는 것으로 추정되었다. Antioxidative effect of tea extracts from green tea (steamed, roasted), oolong tea and black tea was investigated. Antioxidative activities of the crude catechin fraction were the most effective in oolong tea which contained the highest level of (-)-epigallocatechin gallate (EGCg). The water-soluble fraction obtained from oolong tea exhibited binding with more ferrous than copper ions. It showed a synergistic effect when used with an antioxidant such as BHT (0.5㎎) and α-tocopherol (2㎎). Especially, this synergistic effect was exhibited more with BHT than with α-tocopherol. Also steamed green tea, roasted green tea and oolong tea showed remarkable free radical scavenging action. The SC_(50)(the concentration of a water-soluble fraction which is required to scavenge 50% of 100μM 1,1-diphenyl-2-picryl hydrazyl radicals) of steamed and roasted green tea, oolong tea and black tea extacts were 11.3㎍/ml, 11.4㎍/ml, 12.7㎍/ml and 14.9㎍/ml, respectively. It is assumed that the antioxidative activity of tea extracts is due to inhibition of peroxidation free radical scavenging and binding action of ferrous ions by mainly tea polyphenol compounds.

녹차 추출물의 아질산염 분해작용

여생규(Saeng-Gyu Yeo),염동민(Dong-Min Yeum),이동호(Dong-Ho Lee),안철우(Cheol-Woo Ahn),김선봉(Seon-Bong Kim),박영호(Yeung-Ho Park) 한국식품영양과학회 1994 한국식품영양과학회지 Vol.23 No.2

기호음료로서 음용되고 있고, 또 인체에 대한 여러가지 약리적 기능이 보고되어 관심이 모아지고 있는 녹차를 시료로 하여 아질산염 분해작용에 대하여 실험 검토하였다. 녹차 추출물의 수용성획분, 메탄올 가용성 및 침전획분 모두 아질산염 분해작용을 나타내었으며, 특히 메탄올 가용성획분에서 아질산염 분해작용이 큰 것으로 나타났다. 그리고 1, 2 및 3차에 걸쳐 추출하였을 때, 아질산염 분해작용은 뚜렷한 유의적인 차이를 찾아볼 수 없었다. 또한 증제차가 덖음차 보다 아질산염 분해작용이 강하였다. 반응액의 pH 변화에 따른 아질산염 분해작용은 pH 의존성이 컸으며, 특히 pH 1.2에서 높은 분해작용을 나타내었다. 각 시료 모두 농도가 증가할수록, 또 반응시간이 길어질수록 아질산염 분해작용이 높았다. 아질산염 분해속도는 아질산염과 아민과의 반응속도 보다 빠른 것으로 나타났으며, 아질산염 분해인자를 검색한 결과, 페놀성 화합물인 것으로 각각 추정되었다. This paper was investigated to elucidate the nitrite-scavenging action as one of biological actions of green tea extracts. Green tea extracts exhibited remarkable nitrite-scavenging action. Among the green tea extracts, steamed green tea showed higher nitrite-scavenging action than roasted green tea. Nitrite-scavenging actions of green tea extracts were hardly influenced until three times extraction with hot water. Furthermore, the nitrite-scavenging action of green tea extracts showed pH dependent, highest at pH 1.2 and lowest at pH 6.0. The nitrite-scavenging rate in sample extracts / amine / nitrite systems exhibited faster than that in amine / nitrite systems. In order to screen the nitrite-scavenging factors, sample extracts were fractionated with methanol. In both samples, methanol-soluble fractions possessed greater nitrite-scavenging action than methanol-insoluble fractions. The nitrite-scavenging action of methanol-soluble fractions increased with proportion to absorbance at 420㎚ and at 280㎚, while that of green tea extracts increased with the contents of total phenols and absorbance at 280㎚.

녹차, 오룡차, 및 홍차 추출물의 항균효과

여생규(Saeng-Gyu Yeo),안철우(Cheol-Woo Ahn),김인수(In-Soo Kim),박영범(Yeung-Beom Park),박영호(Yeung-Ho Park),김선봉(Seon-Bong Kim) 한국식품영양과학회 1995 한국식품영양과학회지 Vol.24 No.2

차 추출물의 항균작용은 모두 농도가 증가할수록 뛰어났으며, 차 종류 중 비발효차인 증제차 및 볶음차에서 가장 뛰어났다. 차 제조과정 중 발효가 진행될수록 감소하였다. 또한 각 획분 별 항균작용은 차 polyphenol 화합물인 조 catechin 획분에서 가장 높게 나타났으며, 차 종류 중 비발효차인 증제차 및 볶음차와 반발효차인 오룡차에서 높게 나타났다. 그리고 조 catechin 획분에서 차 종류 모두 Gram 양성균인 Micrococcus luteus와 Gram 음성균인 Enterobacter aerogenes 및 Vibrio parahaemolyticus에 대하여 각각 30~50㎍/ml, 50~60㎍/ml 및 60~70㎍/ml의 MIC로 항균효과가 좋은 것으로 나타났다. 특히 충치 원인균인 Streptococcus mutans 균에 대하여는 차 제조시 발효 정도가 큰 차일수록 항균 작용이 떨어지는 것으로 나타났다. 중제차 및 볶음차는 Staphylococcus aureus에 대하여 120~140㎍/ml, Listeria monocytogenes에 대하여 300~400㎍/ml의 항균작용을 나타내었다. 이들 차 추출물의 항균작용에는 차 polyphenol 화합물로서 gallate기가 결합된 구조를 가진 catechin류인 것으로 나타났다. Antimicrobial effect of tea extracts from green tea (steamed, roasted), oolong tea and black tea was investigated. Minimal inhibitory concentration (MIC) of tea extracts against 9 well known strains of foodborne pathogenic bacteria such as the Gram positive and Gram negative bacteria was determined at 37℃. Significant antimicrobial activity was observed in the steamed green tea and the roasted green tea of the water-soluble fraction, and the steamed green tea of the methanol-soluble fraction, and the steamed green tea, roasted green tea and the oolong tea of the crude catechin fraction. The MIC of these tea extracts against B. subtillis were 700㎍/ml, 500㎍/ml and 120㎍/ml, respectively. The crude catechin fraction possessed greater antimicrobial activity than did the other fractions. Among tea extracts, extracts of steamed green tea, roasted green tea and oolong tea showed higher antimicrobial activity than them of black tea. The MIC of the crude catechin fraction obtained from tea extracts against Gram-positive bacteria such as M. luteus, B. subtillis and S. mutans were 30~50㎍/ml, 120~240㎍/ml and 120~180㎍/ml, and against Gram-negative bacteria such as E. aerogenes and V. parahaemolyticus were 50~60㎍/ml and 60~70㎍/ml in the broth medium, respectively. Especially, the MIC to Streptococcus mutans which has known as a causative bacteria of a decayed tooth were 120㎍/ml, 140㎍/ml, 180㎍/ml and above 1,000㎍/ml in steamed green tea, roasted green tea, oolong tea and black tea, respectively. Tea extracts had strong growth inhibition activity against foodborne pathogenic and dental bacteria.

멀티패스 밀폐식 냉각탑의 관경변화 특성

심규진(Gyu-Jin Shim),이호생(Ho-Saeng Lee),김은필(Eun-Pil Kim),윤정인(Jung-In Yoon),권오익(O-Ick Kwon) 대한설비공학회 2008 대한설비공학회 학술발표대회논문집 Vol.2008 No.2

This paper presents experimental result of characteristics about Closed circuit Cooling Tower having a rated 2RT. The experimental apparatus has been set-up with a conventional type system. The test section is heat exchangers of cooling tower that consist of different vertical tubes, 15.88㎜ with 10 rows and columns and 19.05 ㎜ with 8 rows and 12 columns. The main results were summarized as follows : The values of heat and mass transfer coefficients of cooling tower operating with two paths are higher than these with one path. Cooling capacity per unit volume using 15.88㎜ tubes are higher than 19.05㎜ tubes.

The Association of Social Support and Quality of Life in Gynecological Cancer Related of Lymphedema in a Hospital: A Pilot Study

( Hoe Saeng Yang ),( Kwan Lee ),( Do Sung Kim ),( Young Joo Sim ),( Ho Joong Jeong ),( Ghi Chan Kim ),( Won Gyu Kim ) 대한산부인과학회 2011 Obstetrics & Gynecology Science Vol.54 No.7

The aim of this study was to investigate the association of social support and quality of life in patients with gynecological cancer related lymphedema. Methods We interviewed to patients with gynecology related lymphedema (n=27) for psychosocial effects, economic affects by questionnaire that we developed. Short form-36 ver. 2 questionnaire was used to assess quality of life of the patients. Beck`s depression index questionnaire was used to assess mood change of the patients. In addition, we identified support need for patients with gynecology cancer related lymphedema. Data were collected from 2009 to 2010. Results Patients with gynecology cancer related lymphedema have limitations to do lives such as activity of daily living, participate to leisure and exercise. Patients perceived symptoms were heaviness, limitation of range of motion, pain, weakness and feeling expansion in lower extremity. There was negative effect for financial status because of increase medical cost and decrease income. However, there was not affect to relationship with family. Half of the patients have some depression symptoms and there was significant decrease in quality of life such as especially, body pain and social functioning, compared to general population. Conclusion Gynecological cancer related lymphedema have negative effect in quality of life, socioeconomic state and patients needed more social support. Therefore, more attention and social support for patients with gynecology cancer related lymhedema are needed.

원자력발전소(原子力發電所)의 문서정보관리(文書情報管理) 체제(體制)

엄규생,Eom, Gyu-Saeng 한국과학기술정보연구원 과학기술정보센터 1984 Journal of Information Science Theory and Practice Vol.17 No.3

Molecular Authentication of Morus Folium Using Mitochondrial nad7 Intron 2 Region

Jin, Chi-Gyu,Kim, Min-Kyeung,Kim, Jin-Young,Sun, Myung-Suk,Kwon, Woo-Saeng,Yang, Deok-Chun The Plant Resources Society of Korea 2013 한국자원식물학회지 Vol.26 No.3

Morus Folium (Sang-yeop in Korean) is one of the most important Oriental medicinal plants. In Korea, both M. alba and M. cathayana are regarded as the botanical sources for Morus Folium. In order to discriminate M. alba and M. cathayana from their adulterant, M. tricuspidata, mitochondrial NADH dehydrogenase subunit 7 (nad7) intron 2 region was targeted for molecular analysis with universal primers. DNA polymorphisms, including SNP sites, insertions, and deletions, were detected among these three species sequencing data. Based on these DNA polymorphisms, specific primers were designed for the three species respectively. Multiplex PCR was conducted for molecular authentication of M. alba, M. cathayana, and M. tricuspidata with specific primers. The present results indicate that it is possible to identify Morus Folium from its adulterant using mitochondrial nad7 intron 2 region. The established multiplex-PCR system was proved to be effective for identification of Morus Folium. The results indicate that mitochondrial introns can be used for inter-specific polymorphic study, and the described method can be applied for molecular identification of medicinal materials.