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김기범,권대규,이삼철,김성종,정인수,오인혁,김기주,변윤섭,정경락 한국화학공학회 2004 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.42 No.2
본 연구는 급성호흡부전환자를 치료하기 위하여 사용되고 있는 혈관 내 폐 보조장치의 기체전달을 향상시키기 위하여 중공사막에 진동기법을 사용하여 기체전달을 향상시키고자 시도하였다. 그리고 혈관 내 폐 보조장치를 정맥에 삽입하기 전, 혈관 내 폐 보조장치의 설계조건을 실험적 모델을 통하여 기체전달을 예측할 수 있는 예측식을 만들고자 하였다. 실험결과, 본 연구에서는 진동기법이 기체전달을 향상시키는데 효과적임을 확인할 수 있었다. 뿐만 아니라, 충진율과 가징 주파수의 함수관계를 이용하여 기체전달을 예측 할 수 있었다. 실험에 의하여 얻어진 결과는 예측식에 의하여 얻어진 결과와 유사한 결과를 얻었다. 그러므로 본 연구에서는 충진율과 가진 주파수의 함수를 이용하여 VIVLAD의 기체전달을 예측할 수 있었다. In this paper, we tried to improve gas exchange of the vibrating intravascular lung assist device(VIVLAD) using vibrating method in the hollow fiber membrane, for the patients suffering from acute respiratory distress syndrome(ARDS). And we tried to formularize prediction equations to make a prediction about gas transfer for designing intravenous artificial lung assist device, and designed modules under various conditions were studied through an experimental modeling before inserted the artificial lung assist device into as venous. As a result, we are convinced that vibration method is very useful for the has transfer increasing. Also, we can estimate the gas transfer as a function of the packing density and excited frequencies. The gas transfer obtained from the experiment was similar to that from the prediction equation, confirming the usefulness prediction equation. Therefore, we can estimate the gas transfer of the VIVLAD as a function of the packing density and excited frequencies.
Genetic characterization of H9N2 avian influenza virus previously unrecognized in Korea
Gyeong-Beom Heo 대한수의학회 2021 Journal of Veterinary Science Vol.22 No.2
In this study, we describe the isolation and characterization of previously unreported Y280-lineage H9N2 viruses from two live bird markets in Korea in June 2020. Genetic analysis revealed that they were distinct from previous H9N2 viruses circulating in Korea and had highest homology to A/chicken/Shandong/1844/2019(H9N2) viruses. Their genetic constellation showed they belonged to genotype S, which is the predominant genotype in China since 2010, where genotype S viruses have infected humans and acted as internal gene donors to H5 and H7 zoonotic influenza viruses. Active surveillance and control measures need to be enhanced to protect the poultry industry and public health.
Avian influenza Surveillance in wild bird in Korea between 2015 and 2016
( Gyeong-beom Heo ),( Eun-kyoung Lee ),( Yu-na Lee ),( Youn-jeong Lee ),( Mingeun Sagong ),( Myoung-heon Lee ),( Yong-joo Kim ) 대한인수공통전염병학회 2018 창립총회 및 학술대회 초록집 Vol.2018 No.1
Introduction: National surveillance of avian influenza virus (AIV) in Korea has been annually conducted for early detection of highly pathogenic avian influenza (HPAI). In Korea, there have been four times of outbreaks of H5N1 HPAI between 2003 and 2011. During 2014-2015, large epizootics in poultry have been caused by H5N8 HPAI viruses. In this study, we report on the result of a nationwide surveillance for AIV in wild birds in Korea between 2015 and 2016. Methods: Samples were collected by the Livestock Health Control Association and regional veterinary offices. The feces of wild birds were collected in major migratory habitats. Healthy migratory birds were captured and oropharyngeal/cloacal swabs were collected from these birds. Each sample was screened by real-time PCR. AIVs were isolated by inoculation on embryonated SPF chicken egg with samples and the presence of AIV was determined using a hemagglutination assay. For sutyping of AIV, HA and NA genes of the AIV isolates were amplified with gene-specific primers using RT-PCR and confirmed by directly sequencing. Results: Between 2015 and 2016, 28,849 samples were collected from wild birds. Of these, 24,091 of wild bird feces, 4,686 from captured birds, and 72 dead birds. A total of 228 viruses were isolated and the various subtypes were found including H1-H12. The major subtypes were H5 (n=59), H6 (n=39), H4 (n=33) and H3 (n=30). H5 AIV was further classified into four NA subtypes: H5N3 (n=23), H5N6 (n=18), H5N8 (n=16) and H5N9 (n=2). H5N6 and H5N8 subtypes were isolated in wild bird feces (n=18), captured bird (n=10), and dead bird (n=6), respectively. These viruses belonged to clade 2.3.4.4 and confirmed as HPAI virus. The H5N6 HPAI viruses isolated in late 2016 were phylogenetically distinguishable from the clade 2.3.4.4 H5N8 HPAI viruses previously isolated in Korea during 2014-2015. Conclusion: Between 2015 and 2016, viruses of various subtypes, including H5 HPAIVs and LPAIV, were isolated from wild bird through AI surveillance. AI Surveillance in wild birds should be enhanced for earlier detection of AIV introduced by wild birds to implement the effective control measure to prevent HPAI outbreaks in poultry farms.