Micropropagation of the Mongolian medicinal plant Zygophyllum potaninii via somatic embryogenesis

Gun‑Aajav Bayarmaa,이나념,강호덕,Batlai Oyuntsetseg,문흥규 한국식물생명공학회 2018 Plant biotechnology reports Vol.12 No.3

The Mongolian medicinal plant Zygophyllum potaninii has been assessed as an endangered species with regional status. We applied the somatic embryogenesis technique using aseptic in vitro germinants of the plant as an effective propagation technology. The seed germination rate in vitro was 16.5% after 2 weeks of culture. Embryonic calli (EC) and somatic embryos (SEs) were induced using the cotyledon or hypocotyl segments of the germinants. Calli were effectively induced on MS medium supplemented with 0.1 mg/L 2, 4-dichlorophenoxy acetic acid (2, 4-d) and 0.5 mg/L 6-benzylamino purine (BA). The callus was composed of pale yellow or pale green friable cells. SE formed from EC only on Murashige and Skoog medium (MS) with 0.5 mg/L abscisic acid (ABA). Other concentrations of ABA failed to induce SE formation. All SEs germinated in MS medium with different salt levels. However, normal plant conversion was achieved only on half-strength MS medium. The converted plantlets were effectively acclimatized in vitro in sand and transferred to a mixture of sand and perlite (1:1 v/v) in the greenhouse. After 8 weeks of culture, 55.4% of the plants survived. This is a first report of propagating the medicinal desert plant Z. potaninii via somatic embryogenesis and plant regeneration.

향신료와 야채류가 세균의 성장에 미치는 영향(Ⅰ)

이건일,정영건,권오진 효성여자대학교 부설 한국환경위생연구소 1992 환경위생연구 Vol.2 No.1

본 실험은 일반가정의 식탁에서 흔히 볼 수 있는 향신료 및 야채류 14종에 대해 3주의 gram양성 및 4주의 gram 음성균의 성장에 미치는 영향을 조사하였다. 공시균의 NaCl에 대 한 내성은 Lactobacillus casei, Staphylococcus aureus, Enterobacter aeroge-nes는 7%의 NaCl에서도 성장하여 내성이 강하였고, 그외의 공시균은 5-7%의 NaCl에서 성장저해효과가 있었다. 열저항성은 75℃이상%서 공시균의 성장이 억제되어 85℃에서는 Bacillussubtilis, Enterobacter aerogenes를 제외한 공시균은 성장이 완전히 저해되었다. 항생물질의내성은 Pseudomonas aeruginosa가 본 실험에 이용된 모든 항생물질에서 MIC가 400 mg/ml로나타나 개성이 가장 강하였으며 Shigella sp.은 MIC가 100 mg/ml로 내성이 가장 약하였다.마늘에서는 Pseudomonas aeruginosa를 제외 한 모든 공시균에서 , 엽차에서는 Bacillus subtilis,Klebsiella pnewmoniae, Shigella sp.에서, 쑥에서는 Staphylococcus aureus, Enterobacter aeroge-nes, Klebsiella pneumoniae, Pseudomonas aeruginosa, Shigella sp.에서 각각 항균작용이 있었으며, 생육저해 농도는 마늘은 0.5-0.4%, 엽차는 4.0-5.0%, 쑥은 2.0-10.0%로 나타났다. This study was carried out to investigate the influence of food spi-ces and vegetables, which are commonly avaible on the dining table everyday,on bacteria growth. Total 14 kinds of food spices and vegetables were sampledin Kyungsan market in Kyungsan city. 7 strains of bacteria were used in thisexperiment as test strains which were 3 strains of Gram positive bacteria (Bacil-lus subtilis,Lactobacillus casei and Staphylococcus aureus) and Gram negative4 strains (Enterobacter aerogenes, Klebsiella pneumoniae, Pseudomonas aerugi-nosa and Shigella sp.) . The results summerrzed were as ronows: 1. The growth of all test strains except Pseudomonas aeruginosa was inhibi-ted by water extracts of raw garlic with concentrations ranging 0.5% to 4.0%. 2. Bacilus subtilis, Klebsiella pnewmoniae and Shigella sp. where not grownin tea with concentrations ranging 4.0% to 5.0%. 3. Water extracts of wormwood also interfered all strains growth exceptBacillus subtilis of which inhibitory concentrations were ranged 2.0% to 10.0%. 4. In susceptibilities of test strains to NaCl solutions the most sensitivestrain was Bacillus subtilis while Enterobacter aerogeneg was showed strongresistance to each different concentration of this solution. Almost the growthof test strains except Enterobacfer aerogenes were inhibited in over 4% of thissolution. 5. GeneraIBy all of test strains were resisted to penicillin G whereas theyall were susceptible only to streptomycin. Shigella sp. was markedly sensitiveto all of antibiotics used in this experiment and MIC of this strain to them was100 mg/ml while Pseudomonas aeruginosa was strongly tolerable to the all ofantibiotics and its MIC to them was 100+ mg/ml. 6. Bacillus subtilis and Enterobacter aerogenes were able to grow until85℃ in gradually increasing temperature.

집중호우에 따른 저수지 사고 피해조사 및 향후과제

허건 ( Heo¸ Gun ) 한국구조물진단유지관리공학회 2020 한국구조물진단유지관리공학회 학술발표대회 논문집 Vol.24 No.2

국내외적으로 저수지의 사고와 붕괴가 집중호우, 지진, 내부침식 등의 사유로 빈번해지고 있다. 이러한 외적인 하중 외에도 저수지 자체의 노후화로 인하여 그 파괴가능성(probability of failure)이 증가하는 실정이며 이러한 사고와 붕괴는 반복적으로 발생하고 있다. 또한 저수지 하류부의 개발 및 도시화로 저수의 붕괴로 인한 잠재적인 피해(consequences) 역시 증가하고 있다. 이에 시설물의 피해 원인 분석 및 대책수립 프로세스를 강화하고 피해 조사 결과를 DB화하여 체계적으로 관리할 필요가 있다. 더불어 기존 시설물을 위험도(risk) 감소를 위한 유지(안전)관리 기술의 개발도 시급한 실정이다.

중소병원의 의료서비스 마케팅에 관한 연구 : 특히 충남 서천군을 중심으로 with special emphasis to the Sochon-Gun in Chung-Nam Province

김항석,신건호,한영희 군산대학교 지역개발연구소 1999 地域開發硏究 Vol.11 No.-

Customer service marketing has been well developed in business since last several decades. The purpose of this research is to apply service orientation in hospitals. The specific purposes are as follows. First, The process of customer service in business includes medical service delivery, which will be a competitive strategy for the 21st century. Second, more specifically, the degree of customer satisfaction will be explored based on data collected and analysed by questionnaire survey. Third, customer service strategy of small-medium sized hospitals will be presented based on the findings of this research. For the successful completion of the research, the established theories in customer service will be reviewed and questionnaire technique will be adopted to collect data. The following finding indicate some general strategies for better medical service delivery in small and medium sized hospitals. First, finding show the punctuality of service employees and immediate notice of examination results are most preferred elements in service delivery. Second, the study also pointed out the kindness of employees in hospitals need to provide free medical program for the community residents. In conclusion, the research showed some critical issues in hospital management. In humane treatment and kindness of employees are the most chronic problems among many others. The research also indicated give a competitive advantage to small and medium sized hospitals for better service delivery.

생강나무 잎과 가지의 정유성분 비교

고건우, 홍열, 임흥빈 충북대학교 연초연구소 2008 煙草硏究 Vol.21 No.-

-본문참고-

미중 갈등 시대 중국어 교수법의 새로운 방향

박건영 淸州大學校 韓國文化硏究所 2024 人文科學論集 Vol.67 No.-

中美冲突后,韩国的汉语教育大幅下滑。在教育领域,培训时间明显减少,随着学习时间的减少,需要修改教育目标和授课管理基调。本文从这个角度出发,提出问题并提出修改建议,重新设定教育目标。这一教育目标提出了汉语作为外语, 如“对外汉语”学习观念的转变。据此修改的教学方法, 其方向如下。 首先,向汉语初学者提议汉字的延迟学习。因为汉字学习时间长, 所以建议争先使用汉语拼音字母,以此代替,训练听力与口语是,能提高学习的初期效果,这方向更有效率。另外,只有确保拼音教育,才能获得今后在电脑上更容易写汉字,输入汉字的优点。 其次,建议实施层次性、循序渐进的学习。这些层次是根据汉字的频率和单词用法来划分的。使用HSK试题就是逐步积累所使用的汉字和表达方式,以便将适合当前缩减学习条件的量传递给学习者。 最后,教师和学习者都强调了数字文明的加速发展给外语学习带来的变化的认识,比如如何利用‘人工智能’的这一趋势。

창의성경영의 이론적 구축에 관한 연구

신건권 경주대학교 창의력개발연구소 2003 創意力開發硏究 Vol.- No.6

社會體育의 活性化 方案

朴建秉 공주대학교산업개발연구소 2000 産業開發硏究 Vol.8 No.-

It is said that many people notice the physical education and social sports. Because with the social and economic development, a lot of people put their interest into the sports; keeping in their good shape and making use of spare time with sports. And this atmosphere was based on the system supported by the government. After finishing the 1986 Asian Games and the 1988 Seoul Olympics, government has tried to promote life sports in Korea. Accordingly, the authorities paid attention to make various policy for the nation's health. This paper showed the function of social sports and the comparison with the foreign policy.

오디오 디지털-아날로그 변환기용 아날로그 회로설계

신건순 金烏工科大學校 2000 論文集 Vol.21 No.-

본 논문에서는 고음질 오디오 신호 처리를 위한 SC DAC과 SC LPF (Switched-Capacitor DAC and Switched-Capacitor Low-Pass Filter)구조와 전원 잡음 제거 능력이 DC에서 -59dB이상, 3MHz 에서 -34dB이상이고, 온도 계수가 36.5ppm/℃이하인 2.25V/4.5V의 고정밀 전압 기준기를 제시 하였고, 온도 계수가 15ppm/℃인 고정밀 전류원을 제시하였다. 본 논문에서 제시한 아날로그 회 로를 0.8㎛ p-sub CMOS DPDM 공정으로 집적하여 실장 테스트 한 결과, 동적범위는 91~93dB, S/N은 93~95dB, THD+N는 83~85dB를 얻을 수 있었고, 소비 전력은 155mW, 칩면적은 2.64×3.05㎟ 이었다.

시변 스위칭 함수를 이용한 가변구조제어계의 도달기간 제거방법

곽군평,주형민 창원대학교 산업기술연구소 2001 産技硏論文集 Vol.15 No.-

본 논문은 시변 스위칭함수를 이용하여 가변구조제어계의 단점인 도달기간을 제거하는 새로운 방법을 제시한다. 제시된 기법에 의해 전체 제어기간동안의 도달기간이 제거되었다. 효과적인 브러시리스 DC모타의 속도제어를 위해 제안된 시변스위칭 함수를 사용하여 속도 제어기를 설계하였다. This paper presents a new method for removing reaching phase in variable structure control systems using time varying switching function. By proposed method, during the entire control process reaching phase is removed. For effective speed control of trapezoidal type brushless DC motor, a time varying switching function based speed controller is developed as illustration.