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Gui Miao,Guo Junliang,Kong Huanjun,Wu Pan,Shan Jianqiang,Peng Yujiao 한국원자력학회 2023 Nuclear Engineering and Technology Vol.55 No.9
A phenomenological study on CHF in a bilaterally heated annulus with equal heat flux on both sides was experimentally performed. The working fluid of the present test was R-134a. Variation characteristics of CHF and transition of CHF occurrence location were investigated under different pressure, mass flux and quality conditions. With the increase of critical thermodynamic quality, it was found that CHF first occurred on the outer surface of the annulus, then simultaneously occurred on both sides, and finally occurred on the inner surface at relatively high critical quality. After the CHF location transitioned to the inner rod, the sharp fall of CHF in the limiting critical quality region was observed. The critical quality corresponding to the CHF location transition decreased with the increase of mass flux and pressure. Besides, CHF in tube, internally heated, externally heated and bilaterally heated annuli were compared under the same hydraulic diameter conditions. The present study is conducive to improving the understanding of complicated CHF mechanism in bilaterally heated annulus, enriching the experimental database, and providing evidence for developing accurate CHF mechanism model for annuli.
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Xiaomin Liu,Chengbin Yang,Jing Liu,Jianwei Liu,Rui Hu,Hongwei Lian,Guimiao Lin,Liwei Liu,Ken-Tye Yong,Ling Ye 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.4
The optimization of aldose reductase (AR) expression levels and tracking of the AR expression sites within the cell is an essential step in developing a platform for the effective production of aldose reductase inhibitors (ARIs). In this study, we have demonstrated the use of both immunocytochemistry and quantum dots-based immunofluorescence techniques for observing and detecting the expression level and localization of AR in the cytoplasm and cell membrane of a eukaryotic cell model with high levels of AR protein expression. Our results show that high expression levels of human AR can be achieved using the eukaryotic cell model that we have developed. The overexpressed AR can be used for translational studies of hAR and the screening of ARIs. More importantly, the use of the established quantum dots-based immunofluorescence technique in the intracellular labeling of AR allows the determination of the expression and distribution of the AR gene. Overall, the use of the interdisciplinary approach of both genetic engineering and quantum dot-based immunofluorescence allows not only the effective production of a desired protein, but also the determination of the cellular localization of such an expressed protein.
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