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교광휘,진남 남서울대학교 2010 남서울대학교 논문집 Vol.16 No.1-2
This research analyzed the tourism expectation of Shanghai tourists and residents, as well as the differences between tourists and residents’ tourism expectation focusing on 2010 Shanghai EXPO. This study revealed that factor analysis selected 5 tourism expectation factors (infrastructural & environmental factor, positive attitude factor, social phenomenon factor, economic factor and price & real estate factor). Among Shanghai tourists and residents’ demographic characteristics, except gender, the other demographic variables like age, educational level, marital level and Occupation all had obvious differences with the 5 expectation factors; T test analysis also revealed the obvious differences from Shanghai tourists and local residents on the tourism expectation and the visiting decision making. In the end the insufficiency of this study is put forward and recommendations on the future study are also given. Key Words : Mega Event, Tourism Expectation, Visiting Decision Making, Tourists, Residents, Shanghai EXPO
방한 중국관광객이 지각한 서울관광지이미지가 관광만족도와 충성도에 미친 영향에 관한 연구
진남,교광휘,이용일 남서울대학교 2009 남서울대학교 논문집 Vol.15 No.1
Typically we can not see it intangibly in the pre-purchase. When compared tourism product to other products, we swallow the uncertain and risk purchase in large quantity. Therefore, tourists' expectations for tourism attractions based on the image, active and intensive navigation through the tourists' information that they need to travel, they will get the positive and unique tourism attractions and they will select the good image.The study selected Chinese tourists as target population, and tried to find the impacts of tourism destination image of Seoul, tourism satisfaction & tourism loyalty to Seoul. Literature research and survey were used in this study.Among Chinese tourists' demographic characteristics, except gender, age, income, the other variables like educational level, marital level and jobs all have obvious differences with tourism destination image of Seoul.Multi- regression analysis also revealed that tourism destination image of Seoul has a positive correlation with tourism satisfaction & tourism loyalty. In the end the insufficiency of this study is put forward and recommendations on the future study are also given. Key Words : Seoul's Tourism Destination Image, Tourism Satisfaction, Tourism Loyalty, Chinese Tourist
Stability analysis of rock slopes using strength reduction adaptive finite element limit analysis
Guang-hui Chen,Jin-feng Zou,Rui Zhang 국제구조공학회 2021 Structural Engineering and Mechanics, An Int'l Jou Vol.79 No.4
The main aim of this study is to propose an effective approach to conduct the stability analysis of rock slopes. Based on the strength reduction technology, the recent developed adaptive finite element limit analysis is adopted to investigate the stability of rock slopes for the first time. The rock mass strength is described by the latest generalized Hoek-Brown failure criterion. To better estimate the rock slope stability, the rock mass disturbance D and slope angle β are also incorporated into the determination of factor of safety by implementing the disturbance weighting factor ζ and the slope angle weighting factor fβ, respectively. The factors of safety obtained from this study are validated by those from existing works based on two real slope cases. For practical use, the factors of safety for the cases of β=45° in undisturbed rock masses are provided in the form of design charts. Finally, a set of design charts for ζ and a fitting formula for fβ are presented and discussed.
An Improved Method of Maximum Power Point Tracking Strategy for Wind Power Conversion System
CHEN Ran,CHEN Jie,CHEN Jia-wei,CHEN Zhi-hui,GONG Chun-ying,YAN Yang-guang 전력전자학회 2011 ICPE(ISPE)논문집 Vol.2011 No.5
The turbine speed loop adjustment is the key link of the designing of the controller for the fixed pitch variable speed wind energy conversion systems (WECS). Because of the strong nonlinear, big inertia and mechanical damping characteristics, it’s difficult to designing the controller of the wind power systems. In this paper, a small signal model is present, and based on the analyzing; a wind turbine speed loop regulator is designed. Then, a method of tracking the peak power conversion system is proposed, which is independent of the turbine parameters and air density. At last, simulation system is built, and the results of the simulation experiments show that, the performance of the controller algorithm meet the requirements of the MPPT without wind measurement.
Upregulation of STK15 in Esophageal Squamous Cell Carcinomas in a Mongolian Population
Chen, Guang-Lie,Hou, Gai-Ling,Sun, Fei,Jiang, Hong-Li,Xue, Jin-Feng,Li, Xiu-Shen,Xu, En-Hui,Gao, Wei-Shi,Cao, Jian-Ping Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.15
Background: The STK15 gene located on chromosome 20q13.2 encodes a centrosome-associated kinase critical for regulated chromosome segregation and cytokinesis. Recent studies have demonstrated STK15 to be significantly associated with many tumors, with aberrant expression obseved in many human malignancies. The purpose of this study was to investigate expression of STK15 in esophageal squamous cell carcinomas (ESCCs) in a Mongolian population. Methods: Two non-synonymous single nucleotide polymorphisms in the coding region of STK15, rs2273535 (Phe31Ile) and rs1047972 (Val57Ile) were assessed in 380 ESCC patients and 380 healthy controls. We also detected STK15 mRNA expression in 39 esophageal squamous cell carcinomas and corresponding adjacent tissues by real time PCR. Results: rs2273535 showed a significant association with ESCC in our Mongolian population (rs227353, P allele = 0.0447, OR (95%CI) = 1.259 (1.005~1.578)). Real time PCR analysis of ESCC tissues showed that expression of STK15 mRNA in cancer tissues was higher than in normal tissues (p = 0.013). Conclusions: Our study showed that functional SNPs in the STK15 gene are associated with ESCC in a Mongolian population and up-regulation of STK15 mRNAoccurs in ESCC tumors compared adjacent normal tissues. STK15 may thus have an important role in the prognosis of ESCC and be a potential therapeutic target.
( Hui Sun ),( Chen Guang Zhao ),( Xin Tong ),( Yi Peng Qi ) 생화학분자생물학회 2003 BMB Reports Vol.36 No.2
A lectin named AAL has been purified from the fruiting bodies of the edible mushroom Agrocybe aegerita. AAL consisted of two identical subunits of 15.8 kDa, its pI was about 3.8 determined by isoelectric focusing, and no carbohydrate was discerned. Being treated by pyrogultamate aminopeptidase, the blocked N-terminus of AAL was sequenced as QGVNIYNI. AAL agglutinated human and animal erythrocytes regardless of blood type or animal species. Its hemagglutinating activity was unaffected by acid or alkali treatment and demetalization or addition of divalent metals Mg^(2+), Ca^(2+) Zn^(2+). ALL was toxic to mice: its LD50 was 15.85 mg per kilogram body weight by intraperitoneal injection. In this study, two novel activities of AAL were proved. It showed inhibition activity to infection of tobacco mosaic virus on Nicotiana glutinosa. The result of IEF suggested that AAL attached to TMV particles. Mycelia differentiation promotion was the other interesting activity. AAL promoted the differentiation of fruit body primordia from the mycelia of Agrocybe aegeritu and Auricularia polytricha. AAL antiserum was prepared and immunologically cross-reactived with several proteins from five other kinds of mushrooms. These results suggested that AAL probably was a representative of a large protein family, which plays important physiological roles in mushroom.
STUDY ON CHINESE TOURISTS' MOTIVATION AND SATISFACTION TO VISIT SOUTH KOREA
Guang-Hui Qiao,Nan Chen,Yuan-Yuan Guan,Seok-Chool Kim 한국관광학회 2008 International Journal of Tourism Sciences Vol.8 No.1
The purposes of this study are to identify the Chinese tourists' profiles, motivation factors, satisfaction levels and to assess the important detenninants and the likelihood of Chinese tourists revisiting Korea. A structured personal interview was conducted and a systematic sampling approach was used to select 240 respondents who were traveling by air. This study identified the major tourism motivations of Chinese tourists; the relationship between tourists' demographic characteristics and motivation factors; the relationship between demographic characteristics and satisfaction levels; motivation has a positive effect on satisfaction; satisfaction has a positive effect on revisit and motivation has a positive effect on revisit. In the end the limitations of this study are discussed and recommendations for future study are made.
Detection of PCB77 by Indirect Competitive Enzyme-linked Immunosorbent Assay in Sea Sediment Samples
Chen, Han-Yu,Zhuang, Hui-Sheng,Yang, Guang-Xin Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.3
3,3',4,4'-Tetrachlorobiphenyl (IUPAC PCB77) is one of seven indicative polychlorinated biphenyls (PCBs) in the surface sediments. The current study presents a novel polyclonal antibody for the determination of the PCB77 using indirect competitive enzyme-linked immunosorbent assay. Under optimum conditions, PCB77 was determined within the concentration range of 0.01-100 ${\mu}g\;L^{-1}$, with a detection limit of 0.057 ${\mu}g\;L^{-1}$. The assays were tested for their cross-reactivity profiles using 3 selected congeners and 4 Aroclor products. The assays were highly specific for coplanar PCB congeners, but less specific for Aroclor1248. The spiked recoveries from five sediment samples were 86%-114% for PCB77 from ELISA, which were satisfactory. The current study demonstrated that the developed antiserum and immunoassay procedure can be used to detect PCB77 in environmental samples. The results of the sediment analysis were confirmed by conventional GC/ECD.
Chen Bingyao,Jiang Huiping,Wang Hui,Yang Guang,Hao Xiuqing 한국독성학회 2024 Toxicological Research Vol.40 No.2
Polychlorinated biphenyls (PCBs) are bioaccumulative persistent organic pollutants with a great impact on cetaceans. To examine the content of PCBs and their risks to finless porpoises, this study determined the concentrations of seven typical PCB congeners in 56 tissue samples of East Asian finless porpoises (EAFPs) sampled in 2009–2012 from Ningbo (29.8835° N, 122.0644° E), Pingtan (25.5133° N, 119.8172° E) and Lvsi (32.1035° N, 121.6078° E). PCB138, PCB153 and PCB101 were the predominant congeners, accounting for 31.15%, 18.59% and 15.75%, respectively, of all PCBs detected. The content of PCBs increased with age in males but decreased from juveniles to adults in females due to transfer to calves by reproduction and lactation. EAFPs in Ningbo and Pingtan accumulated more PCBs than those in Lvsi Port. The trophic positions of EAFPs from Lvsi, Pingtan and Ningbo were 9.41, 8.95 and 9.43, respectively. PCB concentrations did not accumulate significantly with increasing trophic levels. The risk quotient index indicated that the risk of trichlorobiphenyl (3-PCB), tetrachlorobiphenyl (4-PCB), pentachlorobiphenyls (5-PCB), and hexachlorobiphenyls (6-PCB) to EAFPs in the East China Sea was generally low and within safe limits thus far.
Guang-Hong Chen,Chun-Ping Jen,Ching-Te Huang,Hsin-Hui Wu,Tatyana N. Zamay,Anna S. Zamay 한국바이오칩학회 2014 BioChip Journal Vol.8 No.2
Detecting rare cells, such as circulatingtumor cells (CTCs), circulating fetal cells, and stemcells, is vital during medical diagnostics and characterization. During carcinogenesis, cancer cells detachfrom the primary tumor into the blood stream, becomingCTCs. Typical rare cell samples are consideredany sample that contains less than 1000 target cellsper milliliter. The volumes of microfluidic devicestypically range from several microliters to nanoliters;this is excessively small for experimenting using lowconcentrationsamples. This study involved isolatingcancerous cells in an open-top chamber with sub-millilitervolumes (0.1 mL) of blood samples by using alysis buffer solution for red blood cells (RBCs), as wellas concentrating cells employing the dielectrophoreticforce generated using stepping electric fields,which were produced using a handheld electric modulethat comprised a voltage-frequency converterand an operational amplifier. To increase the samplevolume, an open-top chamber was fabricated on andbonded to a glass substrate by using circular microelectrodes. The concentrations of cancer cells andRBCs were adjusted to 500 cells/mL and 4×105 cell/mL, respectively, for the experiments. To reduce theinterference of blood cells during detection and isolateCTCs, the RBCs in the sample were lysed in alysis buffer solution before the proposed chip wasused to dielectrophoretically manipulate the rare cancerouscells. The findings indicated that the lysis bufferlysed the erythrocytes and the survivability levelsof the cancerous cells (HeLa and MCF-7) remainedhigh in the lysis buffer. The positive dielectrophoreticcancerous cells were guided based on the direction ofthe stepping electric field because of movement in thehigh-electric-field region; hence, the cancerous cellsconcentrated and collected at the central electrode.