http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
High Efficiency Binding Aptamers for a Wide Range of Bacterial Sepsis Agents
( Ana Claudia Graziani ),( Maria Isabel Stets ),( Ana Luisa Kalb Lopes ),( Pedro Henrique Caires Schluga ),( Soledad Marton ),( Ieda Mendes Ferreira ),( Antero Silva Ribeiro De Andrade ),( Marco Aurel 한국미생물 · 생명공학회 2017 Journal of microbiology and biotechnology Vol.27 No.4
Sepsis is a major health problem worldwide, with an extremely high rate of morbidity and mortality, partly due to delayed diagnosis during early disease. Currently, sepsis diagnosis requires bacterial culturing of blood samples over several days, whereas PCR-based molecular diagnosis methods are faster but lack sensitivity. The use of biosensors containing nucleic acid aptamers that bind targets with high affinity and specificity could accelerate sepsis diagnosis. Previously, we used the systematic evolution of ligands by exponential enrichment technique to develop the aptamers Antibac1 and Antibac2, targeting the ubiquitous bacterial peptidoglycan. Here, we show that these aptamers bind to four gram-positive and seven gram-negative bacterial sepsis agents with high binding efficiency. Thus, these aptamers could be used in combination as biological recognition elements in the development of biosensors that are an alternative to rapid bacteria detection, since they could provide culture and amplification-free tests for rapid clinical sepsis diagnosis.
Improvement of Erdheim-Chester disease-related renal failure after treatment with anakinra
( Manuel Alfredo Podesta ),( Giorgio Graziani ),( Francesco Reggiani ),( Michele Buemi ),( Salvatore Badalamenti ),( Claudio Ponticelli ) 대한신장학회 2014 Kidney Research and Clinical Practice Vol.33 No.3
Erdheim-Chester disease (ECD) is a rare non-Langerhans cell histiocytosis characterizedby infiltrates of lipid-laden CD68þ/CD1a histiocytes, affecting heart,lungs, central nervous system, and bones. Kidney and adjacent structures can alsobe affected, leading to renal failure in about 30% of cases. The diagnosis ischallenging, and treatment is generally based on administration of interferonalpha(IFNα), but preliminary results also showed the therapeutic efficacy ofanakinra, an antagonist of the receptor of interleukin-1 (IL-1). We report the caseof an elderly patient with ECD and severe involvement of the heart and kidneys whowas successfully treated with anakinra.
Phenolic Compounds and Antioxidant Activity of Italian Extra Virgin Olive Oil Monti Iblei
Fabio Galvano,Luca La Fauci,Giulia Graziani,Rosalia Ferracane,Roberta Masella,Claudia Di Giacomo,Antonio Scacco,Massimo D'Archivio,Luca Vanella,Giacomo Galvano 한국식품영양과학회 2007 Journal of medicinal food Vol.10 No.4
Beneficial health effects have recently been claimed for pomegranate juice. In vitroand in vivo studies havedemonstrated its anti-atherosclerotic capacity, chemoprevention and chemotherapy of prostate cancer, and antiproliferative,apoptotic, and antioxidant activity, among others. On the other hand, there is a complex interplay between tumor initiation,promotion, and progression and xenobiotic biotranformation. This led us to investigate the effect of pomegranate juice con-sumption on cytochrome P450 (CYP) activity and expression. For this purpose, male mice consumed this fruit juice for 4over, the activity of CYP isoform 2E1 and expression of the main CYP isoforms, namely, CYP1A1/2, CYP2E1, and CYP3A,were also assessed. It was found that pomegranate juice consumption decreased total hepatic CYP content as well as the ex-pression of CYP1A2 and CYP3A. Prevention of procarcinogen activation through CYP activity/expression inhibition may beinvolved in pomegranate juice’s effect on tumor initiation, promotion, and progression.
Hepatic alterations associated with fine particulate matter exposure
Iván Tavera Busso,Ana Carolina Mateos,Alicia González Peroni,Natalia Soledad Graziani,Hebe Alejandra Carreras 한국독성학회 2020 Toxicological Research Vol.36 No.2
Several studies have pointed to fine particulate matter ( PM2.5) as the main responsible for air pollution toxic effects. Indeed, PM2.5 may not only cause respiratory and cardiovascular abnormalities but it may also affect other organs such as the liver. Be that as it may, only a few studies have evaluated the PM2.5 effects on hepatic tissue. Moreover, most of them have not analyzed the relationship between particles composition and toxicological effects. In this study, healthy rats were subjected to urban levels of PM2.5 particles in order to assess their structural and functional effects on the liver. During the exposure periods, mean PM2.5 concentrations were slightly higher than the value suggested by the daily guideline of the World Health Organization. The exposed rats showed a hepatic increase of Cr, Zn, Fe, Ba, Tl and Pb levels. This group also showed leukocyte infiltration, sinusoidal dilation, hydropic inclusions and alterations in carbohydrates distribution. These histologic lesions were accompanied by serological changes, such as increase of total cholesterol and triglycerides, as well as genotoxic damage in their nuclei. We also observed significant associations between several biomarkers and PM2.5 composition. Our results show that exposure to low levels of PM2.5 might cause histologic and serological changes in liver tissue, suggesting that PM2.5 toxicity is influenced not only by their concentration but also by their composition and the exposure frequency.