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One-pot one-step deracemization of amines using ω-transaminases
Shin, Giyoung,Mathew, Sam,Shon, Minsu,Kim, Byung-Gee,Yun, Hyungdon The Royal Society of Chemistry 2013 Chemical communications Vol.49 No.77
<P>In this study, we developed a one-pot one-step deracemization method for the production of various enantiomerically pure amines using two opposite enantioselective ω-TAs. Using this method, various aromatic amines were successfully converted to their (<I>R</I>)-forms (>99%) with good conversion.</P> <P>Graphic Abstract</P><P>In this study, we developed a one-pot one-step deracemization method for the production of various enantiomerically pure amines using two opposite enantioselective ω-TAs. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c3cc43348j'> </P>
A Simple and Facile Screening Method to Discover the Plastic Degrading Microorganisms
Giyoung SHIN,Hyuni JUNG,Sung Yeon HWANG,Jeyoung PARK,Dongyeop X. OH 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
The current decomposition rate of biodegradable plastics cannot satisfactorily curb the global plastic waste crisis. Thus, identifying efficient plastic-digesting microorganisms is necessary to accelerate the decomposition of plastics. However, screening for these microorganisms is labor-intensive and time-consuming. The distribution of hydrophobic plastics homogeneously, with a high surface area and cell accessibility, in a culture medium is challenging. Herein, a high-throughput, straightforward micro-spray-based screening system is presented. Plastic microparticles are sprayed on an agar plate, thereby providing a translucent screening plate. This method is rapid, as opposed to the tedious, intricate, and skilled preparation required for polymer emulsion. Micro-sprayed plastic samples require a minimal effective dose (15 ㎎) and share a large interface with microorganisms. The plastic-degrading activity of microbes results in a transparent clear zone around the colony, thereby being readily recognizable. Our method can accelerate the discovery of novel plastic-degrading microorganisms and be applied to future studies on the decomposition of non-degradable plastics.