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Ghimire, Ganesh,Dhakal, Krishna P,Neupane, Guru P,Gi Jo, Seong,Kim, Hyun,Seo, Changwon,Hee Lee, Young,Joo, Jinsoo,Kim, Jeongyong IOP 2017 Nanotechnology Vol.28 No.18
<P>Organic/inorganic hybrid structures have been widely studied because of their enhanced physical and chemical properties. Monolayers of transition metal dichalcogenides (1L-TMDs) and organic nanoparticles can provide a hybridization configuration between zero- and two-dimensional systems with the advantages of convenient preparation and strong interface interaction. Here, we present such a hybrid system made by dispersing <I>π</I>-conjugated organic (tris (8-hydroxyquinoline) aluminum(III)) (Alq<SUB>3</SUB>) nanoparticles (NPs) on 1L-MoS<SUB>2</SUB>. Hybrids of Alq<SUB>3</SUB> NP/1L-MoS<SUB>2</SUB> exhibited a two-fold increase in the photoluminescence of Alq<SUB>3</SUB> NPs on 1L-MoS<SUB>2</SUB> and the n-doping effect of 1L-MoS<SUB>2</SUB>, and these spectral and electronic modifications were attributed to the charge transfer between Alq<SUB>3</SUB> NPs and 1L-MoS<SUB>2</SUB>. Our results suggested that a hybrid of organic NPs/1L-TMD can offer a convenient platform to study the interface interactions between organic and inorganic nano objects and to engineer optoelectronic devices with enhanced performance.</P>
Embryological studies on <i>Abelia tyaihyoni</i> Nakai (Caprifoliaceae)
Ghimire, Balkrishna,Suh, Gang Uk,Lee, Cheul Ho,Heo, Kweon,Jeong, Mi Jin Elsevier 2018 Flora Vol.242 No.-
<P><B>Abstract</B></P> <P> <I>Abelia tyaihyoni</I> is a Korean endemic species and also designated as an endangered taxon in the IUCN Red List. We present a comprehensive embryology of <I>A. tyaihyoni</I>, comparing it with previously available information on the Caprifoliaceae and related families, and identifying possible evolutionary trends. Overall, comparisons showed that <I>Abelia</I> is similar to the other genera of Caprifoliaceae in many embryological features, including the tetrasporangiate anther, the anther wall, dicotyledonous wall formation, fibrous endothecium, simultaneous cytokinesis, tetrahedral tetrads, and three-celled pollen grains. In addition, <I>Abelia</I> share anatropous, unitegmic, and tenuinucellate ovules, ephemeral antipodals, and a cellular-type endosperm with many caprifoliaceous genera. Some embryological features, such as the amoeboid tapetum with several nuclei forming a polyploid mass, bisporic <I>Allium</I>-type embryo sac, obturator, bilayered endocarp, and compressed exotesta, are here described for the first time in this genus. The results confirm that <I>A. tyaihyoni</I> shares some significant embryological features with other Caprifoliaceae. Although previous embryological studies on the Caprifoliaceae lack convincing evolutionary explanations, our comparative approach demonstrates some possible apomorphies in <I>Abelia</I> such as the presence of two sterile carpels, bisporic <I>Allium</I>-type embryo sac, single-seeded fruit, bilayered and sclerified endocarp, and compressed exotesta.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>Abelia</I> is similar to the other Caprifoliaceae in many embryological features. </LI> <LI> Amoeboid tapetum with several nuclei forming polyploidy mass is new to the genus. </LI> <LI> Bisporic <I>Allium</I> type of embryo sac and obturator formation is also new to the genus. </LI> <LI> <I>Allium</I> embryo sac, bilayered endocarp & compressed exotesta are possible apomorphies for the family. </LI> </UL> </P>
Ghimire, Bimal Kumar,Li, Cheng Hao,Kil, Hyun-Young,Kim, Na-Young,Lim, Jung-Dae,Kim, Jae-Kwang,Kim, Myong-Jo,Chung, Ill-Min,Lee, Sun-Joo,Eom, Seok-Hyun,Cho, Dong-Ha,Yu, Chang-Yeon The Korean Society of Medicinal Crop Science 2007 韓國藥用作物學會誌 Vol.15 No.5
Field performance and morphological characterization was conducted on seven transgenic lines of Codonopsis lanceolata expressing ${\gamma}-TMT$ gene. The shoots were obtained from leaf explants after co-cultivation with Agrobacterium tume-faciens strain LBA 4404 harboring a binary vector pYBI 121 that carried genes encoding ${\gamma}-Tocopherol$ methyltransferase gene (${\gamma}-TMT$) and a neomycin phosphotransferase II gene (npt II) for kanamycin resistance. The transgenic plants were transferred to a green house for acclimation. Integration of T-DNA into the $T_0\;and\;T_1$ generation of transgenic Codonopsis lanceolata genome was confirmed by the polymerase chain reaction and southern blot analysis. The progenies of transgenic plants showed phenotypic differences within the different lines and with relative to control plants. When grown in field, the transgenic plants in general exhibited increased fertility, significant improvement in the shoot weight, root weight, shoot height and rachis length with relation to the control plants. However, all seven independently derived transgenic lines produced normal flower with respect to its shape, size, color and seeds number at its maturity. Indicating that the addition of a selectable marker gene in the plant genome does not effect on seed germination and agronomic performance of transgenic Codonopsis lanceolata. $T_1$ progenies of these plants were obtained and evaluated together with control plant in a field experiment. Overall, the agronomic performance of $T_1$ progenies of transgenic Codonopsis lanceolata showed superior to that of the seed derived non-transgenic plant. In this study, we report on the morphological variation and agronomic performance of transgenic Codonopsis lanceolata developed by Agrobacterium transformation.
Conservation of Biodiversity and Forest Resources in Nepal -Degradation and Threats-
Ghimire, Bimal Kumar,Islam, Mohammad Saiful,Chun, Su-Kyoung Korea Furniture Society 2008 한국가구학회지 Vol.19 No.4
Nepal is rich country in terms of its vast array of biological diversity. The relationship between local communities and the biological diversity in Nepal is a very entangle one, whereby two-thirds of our population are heavily dependent upon the biodiversity for their survival. The destruction of biodiversity signifies the destruction of people's livelihoods and survival. Biodiversity and genetic resources conservation has been a most significant part of the Nepalese society due to its socio-economic and cultural importance and provides the human race trees for timber, fuel, food, industrial raw materials, pharmaceuticals, beverages and environmental stabilization. Threats to biodiversity at global level is of greater concern, specially, human activities are the greatest factor currently affecting biodiversity, through the use of natural resources by industry, agriculture, hunting, and energy generation.
GHIMIRE, BALKRISHNA,LEE, HAYAN,CHOI, GO EUN,JEONG, MI JIN,SUH, GANG UK,LEE, CHEUL HO,HEO, KWEON,SON, SUNG WON Magnolia Press 2016 Phytotaxa Vol.283 No.3
<P>The seed morphology and anatomy of 12 species, representing five sections of Thalictrum, were investigated by scanning electron microscopy (SEM) and light microscopy (LM) to evaluate seed features for use in systematic analysis. Considerable differences were observed in seed surface micromorphology and seed coat characteristics both among and within sections of the genus. MANOVA, MDA, and cluster analysis were used to unravel the morphology of seeds among the species. The characteristic seed features for species were found to be seed shape, size, testa thickness, exotestal cells and seed surface sculpture. The results indicated that the most prevalent seed coat sculpture among the studied species was reticulate and some species were reticulate at the margin and rugose in the center. Seed surface was without ornamentation and epidermal cells were mostly polygonal, rectangular or irregularly shaped. The testa thickness varied from two to six cell layers, and the exotesta was well characterized and rectangular to tangentially elongate in most of the species, except T. simplex and T. uchiyamae in which exotesta was poorly represented. It has been observed that species with a non-reticulate or poorly developed reticulate surface sculpture have a less protected seed coat and vice versa. The results of this study indicated that seed morphological features do not, in general, provide strong support to the sectional division of the genus, but that species within sections shared some comparable seed features.</P>