Effect of bosentan on the production of proinflammatorycytokines in a rat model of emphysema

Gamze Kırkıl,Mehmet Hamdi Muz,Figen Deveci,Teyfik Turgut,Fulya İ,lhan,İ,brahim Ö,zercan 생화학분자생물학회 2007 Experimental and molecular medicine Vol.39 No.5

Endothelin (ET) receptor antagonists have been developed to produce a reduction of ET related effects in various diseases, as well as in animal models of airway inflammation. We aimed to investigate the anti-inflammatory potential of bosentan on a rat model of emphysema. Thirty Wistar male rats were classified as control group (group 1), intratracheally (i.t.) instilled with saline, treated with vehicle solution; elastase group (group 2), i.t. instilled with porcine pancreatic elastase (PPE), treated with vehicle solution; and PPE+bosentan group (group 3), i.t. instilled with PPE, treated with bosentan. The levels of TNF-α, IL-1β, IL-6, and IL-8 in bronchoalveolar lavage fluid (BALF) and lung tissue, cell counts in BALF, and histologic analysis of all groups were evaluated. Neutrophile granulocytes (NG) and alveolar macrophages (AM) were increased more in group 2 than in group 1 (P < 0.001, P = 0.04, respectively). Compared with group 2, neutrophil granulocyte (NG) and alveolar macrophages (AM) counts were decreased in group 3 (P < 0.001). Histological examination confirmed a diffuse neutrophilic inflammation and irregular alveolar air space enlargement in group 2. Treatment with bosentan partially reduced the enlarged lung volumes. Compared with group 1, the BALF levels of TNF-α and IL-6, and the lung tissue levels of IL-1β, IL-6, and IL-8 were increased in group 2 (P = 0.028, P = 0.005, P = 0.001, P = 0.019, P < 0.001, respectively). The TNF-α and IL-8 levels of BALF (P = 0.007, P = 0.001, respectively), and the TNF-α, IL-1β, IL-6, and the IL-8 levels of lung tissue (P = 0.031, P = 0.017, P = 0.007, P < 0.001) were decreased in group 3 compared to group 2. In conclusion, bosentan decreased the inflammatory response by reducing numbers of inflammatory cells and proinflammatory cytokines.

Physiological Responses of the Mediterranean Subtidal Alga Peyssonnelia squamaria to Elevated CO2

Gamze Yıldız 한국해양과학기술원 2018 Ocean science journal Vol.53 No.4

The ecological consequences of ocean acidification are unclear due to varying physiological properties of macroalgae and species-specific responses. Therefore, in the present study, we used a laboratory culture experiment to analyse the eco-physiological responses of the Mediterranean subtidal red alga Peyssonnelia squamaria to CO2-induced lower pH. Our results showed an increase in the photosynthetic performance and growth rate of P. squamaria, despite the reduction in CaCO3 content in the low pH treatment. According to our results, we believe that samples exposed to elevated CO2 could be regulated own nitrogen metabolism to support increased growth rate and it may be down-regulated nitrate uptake. As a result, we hypothesize that P. squamaria may benefit from ocean acidification.

Methacrylate Polymers Having Pendant Chalcone Moieties

Gamze Barim,Ozgul Altun,Mustafa Gokhun Yayla 한국고분자학회 2015 폴리머 Vol.39 No.1

A new methacrylate copolymer that includes chalcone as a side group, poly(4-methacryloyloxyphenyl-4"-methoxystyryl ketone-co-styrene) was synthesized by free radical copolymerization. FTIR and 1H NMR spectroscopic techniques were used to characterize the homopolymers and copolymers. The copolymerizations were carried out to high conversions. Copolymer compositions were established by 1H NMR spectra analysis. The monomer reactivity ratios for copolymer system were determined by the linearized Kelen Tudos, and Extended Kelen Tudos methods and a non-linear least squares method. The molecular weights and polydispersity index of copolymers were measured by using the gel permeation chromatography (GPC). The effect of copolymer compositions on their thermal behavior were studied by differential scanning calorimetry and thermogravimetric analysis methods. The optical properties of the resulting copolymer were also investigated.

Thiol/Disulfide Homeostasis in Bipolar and Unipolar Depression

Gamze Erzin,Güven Özkaya,Canan Topçuoğlu,Rabia Nazik Yüksel,Özcan Erel,Emine Feyza Yurt,Erol Göka,Sinan Gülöksüz 대한정신약물학회 2020 CLINICAL PSYCHOPHARMACOLOGY AND NEUROSCIENCE Vol.18 No.3

Objective: Bipolar disorder and unipolar depressive disorder are complex phenotypes. There appear to be phenotypical, mechanistic, and therapeutic differences between bipolar depression (BD) and unipolar depression (UD). There is a need for understanding the underlying biological variation between these clinical entities. The role of oxidative processes underlying bipolar disorder and depression has been demonstrated. Thiol-disulfide homeostasis (TDH) is a recent oxidative stress marker. In this study, we aimed to inspect patients with bipolar depression and unipolar depression in terms of thiol-disulfide balance and to compare them with healthy controls. Methods: Patients admitted to the outpatient clinic of Ankara Numune Training and Research Hospital and diagnosed either as a depressive episode with bipolar disorder (n = 37) or unipolar depression (n = 24) according to DSM-5 criteria, along with healthy controls (HC) (n = 50), were included in the study. Native thiol, total thiol, and disulfide levels were compared across the groups. Results: In comparison to HC, both BD and UD groups had higher disulfide levels, disulfide/native thiol ratio, and disulfide/total thiol ratio. No significant differences between BD and UD were detected in terms of disulfide level, disulfide/ native thiol ratio, and disulfide/total thiol ratio. Conclusion: Increased levels of disulfide, native thiol, and disulfide/total thiol ratios compared to healthy controls in both UD and BD groups may be indicative of the presence of oxidative damage in these two clinical conditions. To clarify the role of oxidative stress in the pathophysiology of depressive disorders and investigate TDH, longitudinal studies in patients with medication-free UD and BD are required.

Stereotactic MR-guided online adaptive radiation therapy (SMART) for the treatment of liver metastases in oligometastatic patients: initial clinical experience

Gamze Ugurluer,Teuta Zoto Mustafayev,Gorkem Gungor,Banu Atalar,Ufuk Abacioglu,Meric Sengoz,Fulya Agaoglu,Gokhan Demir,Enis Ozyar 대한방사선종양학회 2021 Radiation Oncology Journal Vol.39 No.1

Purpose: We aimed to present our initial clinical experience on the implementation of a stereotactic M2021-04-26methods for treatment of liver metastases in oligometastatic disease with the advantages of better visualization of soft tissue, real-time tumor tracking and potentially reduced toxicity to organs at risk.

Nonlinear finite element analysis of ultra-high performance fiber reinforced concrete beams subjected to impact loads

Gamze Demirtas,Naci Caglar,Yusuf Sumer 국제구조공학회 2022 Structural Engineering and Mechanics, An Int'l Jou Vol.82 No.1

Ultra-high performance fiber reinforced concrete (UHPFRC) is a composite building material with high ductility, fatigue resistance, fracture toughness, durability, and energy absorption capacity. The aim of this study is to develop a nonlinear finite element model that can simulate the response of the UHPFRC beam exposed to impact loads. A nonlinear finite element model was developed in ABAQUS to simulate the real response of UHPFRC beams. The numerical results showed that the model was highly successful to capture the experimental results of selected beams from the literature. A parametric study was carried out to investigate the effects of reinforcement ratio and impact velocity on the response of the UHPFRC beam in terms of midpoint displacement, impact load value, and residual load-carrying capacity. In the parametric study, the nonlinear analysis was performed in two steps for 12 different finite element models. In the first step, dynamic analysis was performed to monitor the response of the UHPFRC beam under impact loads. In the second step, static analysis was conducted to determine the residual load-carrying capacity of the beams. The parametric study has shown that the reinforcement ratio and the impact velocity affect maximum and residual displacement value substantially.

A gas uptake based method for biomass estimation of the cyanobacteria Chlamydomonas asymmetrica in flat panel biofilm photobioreactor

Gamze Er,Alexander Jahn,Abid Ali,Khawaja Muhammad Imran Bashir,Man-Gi Cho 한국수산과학회 양식분과 2021 한국수산과학회 양식분과 학술대회 Vol.2021 No.4

A Gas Uptake and Holdup Based Method for Biomass Estimation of the Cyanobacteria Chlamydomonas asymmetrica in Flat Panel Biofilm Photobioreactor

Gamze ER,Alexander JAHN,Shah Abid ALI,Man-Gi CHO 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.4

Biofilm-based Photobioreactors (PBRs) use microalgal biomass immobilized on a substrate with reduced water content and consumption when compared to conventional suspended cultivation. CO2 and carbonate are used by microalgae as inorganic carbon source and their consumption is an indicator for metabolic activity and biomass accumulation. Here CO2 uptake, holdup and release in and from the culture were measured and correlated with gravimetrically determined biofilm mass. For this, a wicking based flat panel biofilm PBR including CO2/O2 gas control was developed, and the CO2 uptake and holdup determined by CO2 NIR off-gas analysis. The cyanobacteria Chlamydomonas asymmetrica were cultivated on hydrophilic modified expanded UHMWPE plates over circulating AF6 medium at 30°C and fluorescent light illumination about 710.01 µW/cm². CO2 holdup in the system was subtracted from values determined during biofilm cultivation at stationary and growth conditions and correlated with measured biomass. From the gas holdup a minimum feasible cultivation size, dependent on the NIR-CO2 sensor sensitivity, for the use of the developed method was estimated. Results suggest the potential use of gas holdup measurement for biofilm growth assessment in medium and large-scale closed cultivations.

Health Tourism Awareness of Health Professionals : Views on General Perspective and Digital Transformation

Gamze Kose,Ozgur Ates 한국디지털융합학회 2023 IJICTDC Vol.8 No.2

As in every sector, employees contribute to health tourism. Since the subject is health, the approach of health professionals should be positive and their knowledge should be sufficient. It is important when the general awareness is thought and it was connected with the latest trends including even digital transformation. So, the connection among health professionals and the most recent factors regarding awareness should be examined by evaluating accordingly feedback from the area. This research addresses the main theme of health tourism awareness of health professionals and aims to illuminate the perspectives of employees on digital transformation in this dynamic field. The findings illustrate the enrichment of important intersections between health professionals and the expanding field of health tourism.

Turkish Version of the Student Nurse Stress Index: Validity and Reliability

Gamze Sarikoc,Meral Demiralp,Emine Oksuz,Berrin Pazar 한국간호과학회 2017 Asian Nursing Research Vol.11 No.2

Purpose: This study aimed to adapt the Student Nurse Stress Index (SNSI) for the Turkish nursing students and investigate its psychometric properties. Methods: Research was conducted with 152 volunteer female students who attended a university college in Ankara, Turkey. Test-retest reliability was investigated for the scale internal consistency (Cronbach a) and stability. Also, content validity and construct validity of the SNSI were assessed. In order to determine the construct validity of SNSI, explanatory and confirmatory factor analysis was conducted. Results: The Turkish version of SNSI with 15 items comprised four factors (academic load, clinical concerns, personal problems, interface worries). The content validity index (CVI) score was .97. Factor loadings of Turkish version of SNSI varied between .532 and .868. The “personal problems” subscale explained 19.01% of the variance; “clinical concerns” explained 18.51%; “interface worries” explained 15.32%; “academic load” explained 14.14%. The total variance explained was 66.99%. The internal consistency coefficient of the SNSI was .86. Conclusion: Results showed that the SNSI had a satisfactory level of reliability and validity in nursing students in Turkey. Multicenter studies including nursing students from different nursing schools are recommended for the SNSI to be generalized.