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Identification of Trichophyton tonsurans by Random Ampli6ed Polymorphic DNA
Kim, Jeong Aee,Gusmao, Norma Buarque de,Okada, Kaoru,Takaki, Galba Maria de Campos,Fukushima, Kazutaka,Nishimura, Kazuko,Miyaji, Makoto 대한피부과학회 1999 Annals of Dermatology Vol.11 No.3
Background : T. tonsurans is an anthropophilic dermatophyte mostly causing tinea capitis and tinea corporis. In East Asian countries, it has rarely been isolated until now. However, it is necessary for researchers in Asian countries to be more accustomed to T. tonsurans than before because of frequent international sports exchanges nowadays. Objectives : This study was performed to identify T. tonsurans by random amplified polymorphic DNA (RAPD) analysis. Methods : Fifteen strains which were tentatively identified as T. tonsurans in Brazil were identified again by several conventional mycological tests and RAPD analysis. Results : Among 15 Brazilian strains, 3 were identified as T. tonsurans, 8 T. mentagrophytes, 3 T. rubrum and 1 T. raubitschekii by conventional mycological tests. This result was examined again by RAPD analysis. Conclusion : RAPD analysis is considered a rapid and reliable method for identification of T. tonsurans if the procedure is carefully standardized with adequate primers.
Fabiana América Silva Dantas de Souza,Amanda Emmanuelle Sales,Pablo Eugênio Costa e Silva,Raquel Pedrosa Bezerra,Germana Michelle de Medeiros e Silva,Janete Magali de Araújo,Galba Maria de Campos Taka 한국고분자학회 2016 Macromolecular Research Vol.24 No.7
The capacity of fibrinolytic enzymes to degrade blood clots makes them of high relevance in medicine and in the pharmaceutical industry. In this work, forty-three microorganisms of the genus Bacillus were evaluated for their potential to produce fibrinolytic proteases. Thirty bacteria were confirmed as producers of fibrinolytic enzymes, the best results obtained for the strain Bacillus amyloliquefaciens UFPEDA 485. The optimization of the enzyme production conditions was done by a central composite design (CCD) star 23 that allowed to define the optimal conditions for soybean flour and glucose concentrations and agitation rate. The highest fibrinolytic activity (FA) of 813 U mL-1 and a degradation of blood clot in vitro of 62% were obtained in a medium with 2% (w/v) of soybean flour and 1% (w/v) glucose at 200 rpm after 48 h of cultivation, at pH 7.2 and 37 °C. The obtained fibrinolytic enzyme was characterized biochemically. Fibrinolytic activity was inhibited by PMSF (fluoride methylphenylsulfonyl - C7H7FO2S) 91.52% and EDTA (ethylenediaminetetraacetic acid - C10H16N2O8) 89.4%, confirming to be a serine- metallo protease. The optimum pH and temperature were 7.0 and 37 oC, respectively, and the enzyme was stable for 12 h. The fibrinolytic activity at physiological conditions of this enzyme produced by Bacillus amyloliquefaciens UFPEDA 485, as well as its long term stability, demonstrate that it has suitable characteristics for human and veterinary applications, and promises to be a powerful drug for the treatment of vascular diseases.