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암세포에 반응하는 면역 세포에 미치는 Ascorbate의 영향 : Ascorbate의 항암 효과 기작 Mechanism of Anticancer Effects
강영준,정가진 大韓免疫學會 1993 大韓免疫學會誌 Vol.15 No.-
Ascorbate is well-known as the anticancer molecule and has been studied as the anticancer drug, but its action mechanism is not well understood. We examined the anticancer effects and mechanism of ascorbate on EL-4 lymphoma-implanted mice. Intraperitoneal administration of ascorbate expanded the survival of EL-4-bearing mice compared with control group(p<0.05). In vitro treatment of ascorbate did not affect the physiology of tumor cells, but in vivo treatment of ascorbate did on the immune function. Injection of ascorbate into EL-4-implanted mice enhanced the cell-mediated cytotoxicity of NK ceIls(p<0.05), without any significant effect on CTL activity. However, in vitro treatment of ascorbate did not enhance the cytotoxic activity of enriched NK cells. So, it is thought that anticancer effect of ascorbate due to the enhancement of ascorbate may be mediated by the nthar mPrhanicmc
Kim, Hyung-Su,Jeong, Gajin The Microbiological Society of Korea 1995 The journal of microbiology Vol.33 No.4
Induction of antibody production in C3H/He mice by bacterial infection is regulated through the processing exerted by antigen presenting cells. From the studies with Psudomonas aeruginosa, Salmonella typhimurium, and Micrococcus luteu, lipopolysaccharides (LPS) in Gram negative bacteria, which are known to be T-cell independent B cell mitogen, seem to be the major factor stimulating immune responses via activation of macrophages. Activation of macrophage, however, does not seem to correlate with antibody production. M. luteus was easily eliminatd by activated macrophages, while the processed antigens were immediately releasedd into culture medium before presentation. Nevertheless, antigens from Gram positive bacteria, Staphylococcus aureus and Bacillus subtilis, were very very active in chemotaxis and activation of periotoneal macrophages as well as in antien presnetation, while the very nature of the antigens is not yet clearly understood.
Ascorbic acid가 생쥐 복강내 거식세포의 탐식작용과 임파구의 활성에 미치는 영향
이상익,강영준,정가진 大韓免疫學會 1993 大韓免疫學會誌 Vol.15 No.-
Ascorbic acid is the most important water soluble antioxidant as well as a cofactor in mangy hydroxylating reactions. There is a lot of evidence that immune system is sensitive to the level of ascorbic acid, and in our previous report it increased the antibody production of the mouse in vivo In this study we examined the effects of ascorbic acid on the phagocytosis and on the activities of the lymphocytes in mouse system. Murine peritoneal macrophages elicited with caseinate anc treated with ascorbic acid in vivo, showed increased phagocytic activity on the opsonized SRBC bui showed decreased detection of hydrogen peroxide(H202). When treated with ascorbic acid in vitro peritoneal macrophages showed no effect on phagocytosis. To examine the indirect effects of ascorbic acid on phagocytosis via lymphocytes, phorbol myristate acetate(PMA) -treated thymocy tes or T cells were incubated with ascorbic acid and macrophage activating capacity was assessed Culture supernatants of ascorbic acid-treated thymocytes and T cells enhanced the phagocytic activity of macrophages at the concentration of lug/ml. The proliferation of the thymocytes anc the blast formation of B cells were correlated with the level of ascorbic acid. Furthermore, ascorbic acid increased antibody production of splenocytes or B cells in vitro, confirming that it affect; immune system via lymphocytes. With the present results, we can explain in part the broad effect, of ascorbic acid on immune system by its influence on lymphocytes.
복수암 Sarcoma 180을 생쥐의 B 임파구 분화에 미치는 Ascorbate의 영향
강영준,노정혜,강사욱,정가진 大韓免疫學會 1993 大韓免疫學會誌 Vol.15 No.-
Ascorbate is one of the important radical scavengers. Its roles in biological system including anti-cancer effects have been broadly studied. Ascorbate recovered the function of B lymphocytes which was suppressed by Sarcoma 180-derived immunosuppressive factor(s). Production of anti-SRBC antidody was increased by the adminisration of ascorbate in the Sarcoma 180 culture supernatant-treated mice. Ascorbate enhanced the suppressed immune function of tumor-bearing hosts. Treatment of 1 mg of ascorbate increased the function of B lymphocytes, however, 10ug of ascorbate treatment decreased. In vitro treatment of ascorbate increased the immunoglobulin production ac-cording to the increasement of ascorbate concentration. These results also imply that ascorbate may be a immunoreeulatory molecule.
Identification of hemolysin as one of the important virulent factors in vibrio anguillarum V7
Choe, Young-Chool,Jeong, Gajin The Microbiological Society of Korea 1995 The journal of microbiology Vol.33 No.4
We have identified hemolysin rendering virulency of Vibrio anguilarum grown at 23.deg.C which was evaluated on human RBCs. Hemolysin itself was separated as a single band on non-denaturing gel electrophoresis. Vibrio hemolysin was destroyed by trypsin and proteinase K and was heat labile. Optimal pH for activity was around pH 6 while pI of the molecule was recognized as 5.7, with relative distance (R$\sub$f/) on non-denaturing gel was 0.7. Addition of EDTA and FeCI$\sub$3/ drew the possibility that the production of hemolysin was mainly induced to overcome iron deficiency inside host animalsd infection.
전염성 췌장 괴저 바이러스의 어류세포에의 감염에 미치는 리소솜 친화성 약염기의 영향
박정우,하영칠,정가진 ( Jeong Woo Park,Yung Chil Hah,GajIn Jeong ) 생화학분자생물학회 1990 BMB Reports Vol.23 No.4
We investigated the effect of ammonium chloride, which is known to affect lysosome-mediated viral internalization, on the infectious of fish cells, CHSE-214, with Infectious Pancreatic Necrosis Virus (IPNV). Progeny production of IPNV was delayed 6 to 8 hours in IPNV infected CHSE-214 cells by the addition of NH₄C1 at the final concentration of 20 mM. The multiplication of IPNV, however, was no longer inhibited when NH₄C1 was present only for the first 4 hours after viral infection. Thus the inhibitory effect of NH₄C1 seems to be on some other steps but internalization. For the inhibitory effect of NH₄C1, time of addition is not very important, but the length of treatment is rather critical. Ammonium chloride attenuated the synthesis of almost all the proteins in IPNV-infected CHSE-214 cells. Thus the primary effect of NH₄C1 on virus-infected host cells is to inhibit the synthesis of proteins resulting in the delay of IPNV growth. Therefore, we conclude that IPNV penetration into CHSE-214 cells does not require the low-pH dependent activity of lysosome.