Clinicopathologic Patterns of Adult Renal Tumors in Pakistan

Hashmi, Atif Ali,Ali, Rabia,Hussain, Zubaida Fida,Faridi, Naveen Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.5

Background: Renal cancer is a serious public health problem which may be under reported and registered in our setup, since the Karachi cancer registry documented only 43 cases out of 4,268 incident cancer cases over 3 year duration. Therefore we aimed to determine the clinicopathologic characteristics of adult renal tumors in our setup. Materials and Methods: The study was conducted in histopathology department, Liaquat National Hospital and included total of 68 cases of adult renal tumors over 4 years. Detailed histopathologic characteristics of tumors were analyzed. Results: Mean age of patients was 56.4 (18-84) years. Renal cell carcinoma (RCC) was the most common cell type (78%) cases; followed by transitional/urothelial carcinoma (12.5%), leiomyosarcoma (4.7%), oncocytoma (1.6%), squamous cell carcinoma (1.6%) and high grade pleomorphic undifferentiated sarcoma (1.6%). Among 50 RCC cases; 62% were conventional/clear cell RCC (CCRCC) type followed by papillary RCC(PRCC), 24%; chromophobe RCC(CRCC), 6% and sarcomatoid RCC(SRCC), 8%. Mean tumor size for RCC was 7.2 cm. Most RCCs were intermediate to high grade (60% and 40% respectively). Capsular invasion, renal sinus invasion, adrenal gland involvement and renal vein invasion was seen in 40%, 18%, 2% and 10% of cases respectively. Conclusions: We found that RCC presents at an earlier age in our setup compared to Western populations. Tumor size was significantly larger and most of the tumors were of intermediate to high grade. This reflects late presentation of patients after disease progression which necessitates effective measures to be taken in primary care setup to diagnose this disease at an early stage.

Rapid assessment of heavy metal-induced toxicity in water using micro-algal bioassay based on photosynthetic oxygen evolution

Fida Hussain,Heonseop Eom,Umair Ali Toor,Chang Soo Lee,Sang-Eun Oh 대한환경공학회 2021 Environmental Engineering Research Vol.26 No.6

Toxicity assessment using microalgae adopted various endpoint measurements like mortality rate, photosynthetic activity, chlorophyll content, enzymatic activity, cell density, <SUP>14</SUP>C assimilation, and phosphate uptake. These algal toxicity tests usually require 3-4 days of exposure time and laborious work to measure these endpoints parameters. In the present study, we described a simple and rapid toxicity assessment procedure using photosynthetic oxygen evolution as an endpoint measurement to determine heavy metal-induced toxicity. Oxygen evolution in gaseous phase was measured over a 12 h exposure time. The toxicity of six selected heavy metals was assessed. Concentrations of 1.02, 1.91, 0.46, 3.2, 7.5, and 65.6 mg/L were obtained as EC50 values for mercury, silver, cyanide, copper, cadmium, and hexavalent chromium, respectively, for microalgae (Chlorella vulgaris). Our results demonstrated that hexavalent chromium is less sensitive depending on the conditions (low exposure time, pH 6, and high initial biomass). Toxicities in decreasing order of strength were cyanide > mercury > silver > copper > cadmium > hexavalent chromium. Results obtained in the current and earlier studies demonstrate that photosynthetic oxygen evolution offers an alternative endpoint measurement to assess toxicity since it allows sensitive and rapid detection of heavy metal-induced toxicity in water.

Rapid assessment of heavy metal-induced toxicity in water using micro-algal bioassay based on photosynthetic oxygen evolution

Fida Hussain,엄헌섭,Umair Ali Toor,이창수,Sang-Eun Oh 대한환경공학회 2021 Environmental Engineering Research Vol.26 No.6

Toxicity assessment using microalgae adopted various endpoint measurements like mortality rate, photosynthetic activity, chlorophyll content, enzymatic activity, cell density, 14C assimilation, and phosphate uptake. These algal toxicity tests usually require 3–4 days of exposure time and laborious work to measure these endpoints parameters. In the present study, we described a simple and rapid toxicity assessment procedure using photosynthetic oxygen evolution as an endpoint measurement to determine heavy metal-induced toxicity. Oxygen evolution in gaseous phase was measured over a 12 h exposure time. The toxicity of six selected heavy metals was assessed. Concentrations of 1.02, 1.91, 0.46, 3.2, 7.5, and 65.6 mg/L were obtained as EC50 values for mercury, silver, cyanide, copper, cadmium, and hexavalent chromium, respectively, for microalgae (Chlorella vulgaris). Our results demonstrated that hexavalent chromium is less sensitive depending on the conditions (low exposure time, pH 6, and high initial biomass). Toxicities in decreasing order of strength were cyanide > mercury > silver > copper > cadmium > hexavalent chromium. Results obtained in the current and earlier studies demonstrate that photosynthetic oxygen evolution offers an alternative endpoint measurement to assess toxicity since it allows sensitive and rapid detection of heavy metal-induced toxicity in water.

Combined application of biochar, compost, and bacterial consortia with Italian ryegrass enhanced phytoremediation of petroleum hydrocarbon contaminated soil

Hussain, Fida,Hussain, Imran,Khan, Aqib Hassan Ali,Muhammad, Yousaf Shad,Iqbal, Mazhar,Soja, Gerhard,Reichenauer, Thomas Gerhard,Zeshan, Thomas Gerhard,Yousaf, Sohail Elsevier 2018 Environmental and experimental botany Vol.153 No.-

<P><B>Abstract</B></P> <P>Petroleum hydrocarbons are extensively utilized in petrochemical industries and cause soil deterioration during exploration, transportation, refining and making petroleum products. We hypothesized that the combined use of compost, biochar and bacterial consortia as soil amendments may enhance the rhizoremediation potential of ryegrass by strengthening the plant rhizospheric effect for efficient total petroleum hydrocarbon removal. The present study focused on phytoremediation of hydrocarbons in spiked contaminated soil amended with biochar (5% v/v), and compost (5% v/v). Spiked soil was inoculated with consortia of four hydrocarbon degrading bacterial strains (<I>Pseudomonas poae, Actinobacter bouvetii, Stenotrophomonas rhizophila</I> and <I>Pseudomonas rhizosphaerae</I>). The spiked soil was prepared by spiking agricultural soil with 3.4% (w/w) of crude oil. Italian ryegrass (60 seeds pot<SUP>−1</SUP>) were sown and plants were harvested after 75 days.</P> <P>The highest hydrocarbon removal (85%) was observed in spiked soil amended with compost, biochar and consortia. Bacterial inoculation with biochar and compost showed significantly higher hydrocarbon degradation as compared to all other treatments. Highest TPHs degrading bacteria (5.74 × 10<SUP>7</SUP> cells g<SUP>−1</SUP> of soil) were observed in rhizosphere of spiked soil amended with compost, biochar and consortia. The organic amendments improved plant growth and bacterial count in rhizosphere which resulted in higher removal of hydrocarbons. We concluded that plant-microbe interactions together with the organic soil amendments offer an emerging trend for remediation of hydrocarbons. Rhizoremediation is a green solution to overcome the quandary of total petroleum hydrocarbon contamination in soil.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Biochar and compost amendment enhanced rhizosphere effect. </LI> <LI> TPHs Rhizoremediation is improved by bacterial consortia and organic amendments. </LI> <LI> The organic amendments improved plant growth and bacterial count in rhizosphere. </LI> <LI> Compost is a rich source of bacteria in the rhizosphere. </LI> </UL> </P>

Accuracy of Frozen Section Analysis of Sentinel Lymph Nodes for the Detection of Asian Breast Cancer Micrometastasis - Experience from Pakistan

Hashmi, Atif Ali,Faridi, Naveen,Khurshid, Amna,Naqvi, Hanna,Malik, Babar,Malik, Faisal Riaz,Fida, Zubaida,Mujtuba, Shafaq Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.4

Background: Intraoperative sentinel lymph node biopsy has now become the standard of care for patients with clinically node negative breast cancer for diagnosis and also in order to determine the need for immediate axillary clearance. Several large scale studies confirmed the diagnostic reliability of this method. However, micrometastases are frequently missed on frozen sections. Recent studies showed that both disease free interval and overall survival are significantly affected by the presence of micrometastatic disease. The aim of this study was to determine the sensitivity and specificity of intraoperative frozen section analysis of sentinel lymph nodes (SLNs) for the detection of breast cancer micrometastasis and to evaluate the status of non-sentinel lymph nodes (non-SLNs) in those patients subjected to further axillary sampling. Materials and Methods: We performed a retrospective study on 154 patients who underwent SLN biopsy from January 2008 till October 2011. The SLNs were sectioned at 2 mm intervals and submitted entirely for frozen sections. Three levels of each section submitted are examined and the results were compared with further levels on paraffin sections. Results: Overall 40% of patients (62/154) were found to be SLN positive on final (paraffin section) histology, out of which 44 demonstrated macrometastases (>2mm) and 18 micrometastases (<2mm). The overall sensitivity and specificity of frozen section analysis of SLN for the detection of macrometastasis was found to be 100% while those for micrometastasis were 33.3% and 100%, respectively. Moreover 20% of patients who had micrometastases in SLN had positive non-SLNs on final histology. Conclusions: Frozen section analysis of SLNs lacks sufficient accuracy to rule out micrometastasis by current protocols. Therefore these need to be revised in order to pick up micrometastasis which appears to have clinical significance. We suggest that this can be achieved by examining more step sections of blocks.

Distribution of Ki67 Proliferative Indices among WHO Subtypes of Non-Hodgkin's Lymphoma: Association with other Clinical Parameters

Hashmi, Atif Ali,Hussain, Zubaida Fida,Faridi, Naveen,Khurshid, Amna Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.20

Background: Non-hodgkin lymphoma (NHL) is a diverse group of disease encompassing divergent tumor types with contrasting clinical behaviors. We aimed to evaluate the usefulness of Ki67 index in segregating indolent from aggressive NHL and its association with clinical parameters. Materials and Methods: During a study period of 4.5 years, a total of 215 cases of lymphomas were diagnosed among of which 172 cases were NHL. Ki67 immunohistochemical staining was performed by the DAKO envision method. Average proportion of tumor cells stained was calculated to determine the proliferative index. Results: The mean age at diagnosis was 46.2 years +19.8 (3-81) with a male to female ratio of 1.5:1. Mean Ki67 index for indolent NHL included 23% for small cell, 25% for mantle cell, 28.5% for marginal zone and 34.6% for follicular lymphoma. On the other hand, mean Ki67 index for aggressive lymphomas were 66.4%, 66.9%, 80.3%, 83.3% and 94.4% for diffuse large B cell, T cell (NOS), anaplastic large cell, lymphoblastic and burkitts lymphoma respectively. No significant correlation was found between Ki67 index and other clinical parameters like age and extra nodal involvement. Conclusions: Ki67 index is a valuable IHC marker to distinguish indolent from aggressive lymphomas especially in small needle biopsies where exact typing may not be possible.

Interface state density and barrier height improvement in ammonium sulfide treated Al2O3/Si interfaces

Agrawal Khushabu,Patil Vilas,Ali Fida,Rabelo Matheus,우원종,조은철,Yi Junsin 한국물리학회 2021 Current Applied Physics Vol.26 No.-

The HF treatment removes the native oxide and lays behind the dangling bonds over the Si surface which causes the increment in density of interface traps (Dit) through the direct deposition of high-k dielectric on Si. Here, we propose the facile method for reduction of interface traps and improvement in barrier height with the (NH4)2S treatment on Al2O3/Si interfaces, which can be used as the base for the non-volatile memory device. The AFM was used to optimize the treatment time and surface properties, while XPS measurements were carried out to study the interface and extract the barrier height (ΦB). The short period of 20 s treatment shows the improvement in the barrier height (1.02 eV), while the one order reduction in the Dit (0.84 × 1012 cm2/eV) of sulfur passivated Al/Al2O3/Si MOS device. The results indicate the favorable passivation of the dangling bonds over the Si surfaces covered by sulfur atoms.

Human health implications, risk assessment and remediation of As-contaminated water: A critical review

Shakoor, Muhammad Bilal,Nawaz, Rab,Hussain, Fida,Raza, Maimoona,Ali, Shafaqat,Rizwan, Muhammad,Oh, Sang-Eun,Ahmad, Sajjad Elsevier 2017 Science of the Total Environment Vol.601 No.-

<P><B>Abstract</B></P> <P>Arsenic (As) is a naturally occurring metalloid and Class-A human carcinogen. Exposure to As via direct intake of As-contaminated water or ingestion of As-contaminated edible crops is considered a life threatening problem around the globe. Arsenic-laced drinking water has affected the lives of over 200 million people in 105 countries worldwide. Limited data are available on various health risk assessment models/frameworks used to predict carcinogenic and non-carcinogenic health effects caused by As-contaminated water. Therefore, this discussion highlights the need for future research focusing on human health risk assessment of individual As species (both organic and inorganic) present in As-contaminated water. Various conventional and latest technologies for remediation of As-contaminated water are also reviewed along with a discussion of the fate of As-loaded waste and sludge.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Arsenic is recognized as a Class-A human carcinogen. </LI> <LI> Groundwater As contamination has affected over 200 million people worldwide. </LI> <LI> This paper reviews current knowledge regarding As in the environment. </LI> <LI> A critical assessment of remediation of contaminated water is presented. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

High mobility WSe2 Field Effect Transistors by Surface Charge Injection

Ngo Tien Dat,Yang Zheng,Fida Ali,Sungwon Lee,Won Jong Yoo 한국진공학회 2020 한국진공학회 학술발표회초록집 Vol.2020 No.2