Silencing of long noncoding RNA PVT1 inhibits podocyte damage and apoptosis in diabetic nephropathy by upregulating FOXA1

Dong-Wei Liu,Jia-Hui Zhang,Feng-Xun Liu,Xu-Tong Wang,Shao-Kang Pan,Deng-Ke Jiang,Zi-Hao Zhao,Zhang-Suo Liu 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

The number of patients with diabetic nephropathy (DN) is still on the rise worldwide, and this requires the development of new therapeutic strategies. Recent reports have highlighted genetic factors in the treatment of DN. Herein, we aimed to study the roles of long noncoding RNA (lncRNA) plasmacytoma variant translocation 1 (PVT1) and histone 3 lysine 27 trimethylation (H3K27me3) in DN. A model of DN was established by inducing diabetes in mice with streptozotocin. Mouse podocyte clone 5 (MPC5) podocytes and primary podocytes were cultured in normal and high glucose media to observe cell morphology and to quantify PVT1 expression. The roles of PVT1 and enhancer of zeste homolog 2 (EZH2) were validated via loss-of-function and gain-of-function in vitro experiments to identify the interactions among PVT1, EZH2, and forkhead box A1 (FOXA1). The podocyte damage and apoptosis due to PVT1 and FOXA1 were verified with in vivo experiments. PVT1 was highly expressed in MPC5 and primary podocytes in DN patients and in cultures grown in high glucose medium. A large number of CpG (C-phosphate-G) island sites were predicted at the FOXA1 promoter region, where PVT1 recruited EZH2 to promote the recruitment of H3K27me3. The silencing of PVT1 or the overexpression of FOXA1 relieved the damage and inhibited the apoptosis of podocytes in DN, as was evidenced by the upregulated expression of synaptopodin and podocin, higher expression of Bcl-2, and lower expression of Bax and cleaved caspase-3. The key findings of this study collectively indicate that the suppression of lncRNA PVT1 exerts inhibitory effects on podocyte damage and apoptosis via FOXA1 in DN, which is of clinical significance.

Quantitative Evaluation Index for Analysis of Assembly Effect on Shield Tunnel Segment Structures

Xun Liu,Kun Feng,Chuan He,Haihua Zhang 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.9

The local force and deformation of shield tunnel will be transferred and redistributed owing to the discontinuous segmental structure. This study develops quantitative evaluation index to explore the influence of the axial compression ratio, eccentricity, and longitudinal force on the assembly effect. A full-scale test is conducted on a single segment and staggered assembled segmental linings. First, the results show that the assembly effect has an obvious redistribution influence on the force and deformation of the segment. Second, the influence of eccentricity on the bending moment is much larger than that of the axial force. The stiffness between rings varied at different positions and the larger the stiffness difference, the more obvious the assembly effect. The results demonstrate that the design and mechanical performance of the shield tunnel under different embedded conditions can be optimized by increasing the longitudinal force, which moderates the assembly effect and ensures the structure reaches the highest bearing capacity.

High-Strength Model Material Production for Structural Plane Replica and Its Shear Testing

Feng Ji,Changjiang Liu,Yu Zhang,Luobing Zheng,Kai Pan,Xun Tan 대한토목학회 2018 KSCE JOURNAL OF CIVIL ENGINEERING Vol.22 No.1

Shear strength parameters (c, φ) of a structural plane are the key factors for the stability assessment of rock masses. The shear strength parameters are obtained by a structure plane shear test carried out in laboratory. However, it is difficult to obtain test samples with the same surface morphology and to remove the effect of surface irregularity from test results. Based on the similarity principle and orthogonal test, this paper presents a new model preparation method for structural planes using a high-strength mold mixture material. Firstly, the original morphology of the structural plane is recorded by in situ measurement using a laser device and then the mold of the structural plane is reproduced using a 3D printer. Subsequently, a mix proportion test of the high-strength mold material is conducted using the orthogonal test, and the model of the structural plane is prepared by pouring this high-strength mixture material into the 3D mold. Ultimately, the shear strength parameters of this high-strength structural plane replica are obtained using a shear box test in the laboratory. The proposed method has particular advantages such as the preparation of multiple replicas for structure planes and the ability to obtain repeatable results.

Agglomeration of particles during coal combustion in multistage spouted fluidized tower

Jia-Xun Liu,Jian-Min Gao,Xiao-Feng Wang,Shao-Hua Wu,Ji-Hui Gao 한국화학공학회 2009 Korean Journal of Chemical Engineering Vol.26 No.3

An experimental platform of spray agglomeration has been designed and built for removing small fly ash particles (PM10) from coal combustion. Systematic experiments were conducted in a multistage spouted tower using kinds of agglomerant solutions. The particle concentration increases greatly from the first stage to the second stage of the tower. With the increase of flue gas flow rate the oscillation of impulse signal response curves increases and the internal circulation of the tower intensifies. The influencing factors such as the surfactant, PH value, flow rate of the agglomerant solutions and inlet flue gas temperature were analyzed. SEM was used to analyze the microstructure of the particles. Final results indicate that the special shape of a multistage spouted fluidized tower has significant influences on the effect of agglomeration. The findings from this work will be helpful to form the basis, and provide guidance for, further studies on the control of fine particles such as PM2.5 or even smaller.

Effect of Different Inter-Stop Transport Distances of a Chinese Freight Train on Its Transport Efficiency

Xuesong Feng,Yuanpeng Jie,Haidong Liu,Xun Sun,Bin Xu 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.6

Utilizing a computer-aided simulation approach, this work studies the impacts of different inter-stop transport distances on the transport efficiency evaluated by the transport time cost intensity of a typically composed Chinese freight train hauled by representative types of locomotives in view of different target speeds. It is found that the decelerated decrease of the transport time cost intensity with improving the target speed is much traded off by decreasing the stop-spacing especially below approximately 20.00 km. Moreover, the decrease of the inter-stop transport distance especially shorter than about 20.00 km obviously increases the transport time cost intensity in an accelerated way for the same target speed. Such a trend is more apparent when the target speed becomes relatively high. Therefore, it is suggested that the inter-stop transport distance of a freight train ought to be over 20.00 km for relatively high transport efficiency.

Two Neutral Copper(I) Complexes Bearing 2-(4-Nitrophenyl) Imidazole[4,5-f]-1,10-Phenanthroline: Synthesis, Crystal Structure, and Luminescence Properties

Xin Fang Liu,Xiao Yu Zhang,Rong Fang Li,Xun Feng 대한화학회 2016 Bulletin of the Korean Chemical Society Vol.37 No.3

Two neutral copper(I) complexes (1 and 2) bearing deprotonated 2-(4-nitro phenyl) imidazole[4,5-f]-1,10-phenanthroline (NPIP) and different phosphine ligands (bis[2-(diphenylphosphino) phenyl]ether (DPEphos) and PPh3) were synthesized and characterized. The molecular structure of a representative complex (1) was established by single-crystal X-ray diffraction. The complex features the metal in a distorted tetrahedral environment surrounded by two N atoms of the chelating NPIP ligand and two P atoms from DPEphos ligand. The emission spectra show that the two complexes exhibit yellow emission both in anhydrous ether and in the solid powder form. Density functional theory (DFT) calculation was carried out at the B3LYP/6-31G** level to determine the electronic transition within the molecule.

Large-scale Landslides in Diexi Region of Minjiang River, Sichuan Province, China

( Yunsheng Wang ),( Bo Zhao ),( Xun Zhao ),( Qianqian Feng ),( Fengshuang Guo ),( Yue Luo ),( Jiangwei Liu ) 대한지질공학회 2019 대한지질공학회 학술발표회논문집 Vol.2019 No.2

The field survey regarding large-scale landslide (Scale) of whole Minjiang River shows that Diexi region, located in upper reach of Minjiang River, is an obvious large-scale landslide concentration area. There are 21 large-scale landslides in Diexi region, and 70% landslides belong to super large-scale landslides (Scale), as shown in Figure 1. All these landslides both blocked rivers and some barrier lakes still exist. Based on field investigations, the failure mechanism and geological environment are discussed in this study. For failure mechanism, three fracture planes cut the rock mass into separation; the stepped fractures forms slip surfaces; finally, these unstable rock mass slide. For geological environment, Diexi region is in west boundary of Minshan block, and in the N-S seismic belt and the Jiaochang arc tectonic belt, two active fractures (Songpinggou fracture and Minjiang fracture) confluence at Diexi region; all these make Diexi region be a Knick point of Minjiang River, a compression and torsion zone and a high stress concentration zone. Intensive uplift and quick incision (1.88 mm/yr.) since the middle Pleistocene formed a gorge with sharp slopes and strong lateral uploading. For failure cause, the intensive tectonic activities make the rock mass produce many fractures, numerous strong earthquakes weaken the rock mass integrity further; finally, the strong earthquake triggered them.

RESULTS ON MEROMORPHIC FUNCTIONS SHARING THREE VALUES CM IN SOME ANGULAR DOMAINS

Li, Xiao-Min,Liu, Xue-Feng,Yi, Hong-Xun Korean Mathematical Society 2016 대한수학회논문집 Vol.31 No.3

We study the uniqueness question of transcendental meromorphic functions that share three values CM in some angular domains instead of the whole complex plane. The results in this paper extend the corresponding results in Zheng [13, 14] and Yi [12]. Some examples are given to show that the results in this paper, in a sense, are the best possible.

Charge density wave order in 1D mirror twin boundaries of single-layer MoSe₂

Barja, Sara,Wickenburg, Sebastian,Liu, Zhen-Fei,Zhang, Yi,Ryu, Hyejin,Ugeda, Miguel ,M.,Hussain, Zahid,Shen, Zhi-Xun,Mo, Sung-Kwan,Wong, Ed,Salmeron, Miquel ,B.,Wang, Feng,Crommie, Michael F. NATURE PUBLISHING GROUP 2016 NATURE PHYSICS Vol.12 No.8

We provide direct evidence for the existence of isolated, one-dimensional charge density waves at mirror twin boundaries (MTBs) of single-layer semiconducting MoSe<SUB>2</SUB>. Such MTBs have been previously observed by transmission electron microscopy and have been predicted to be metallic in MoSe<SUB>2</SUB> and MoS<SUB>2</SUB>. Our low-temperature scanning tunnelling microscopy/spectroscopy measurements revealed a substantial bandgap of 100 meV opening at the Fermi energy in the otherwise metallic one-dimensional structures. We found a periodic modulation in the density of states along the MTB, with a wavelength of approximately three lattice constants. In addition to mapping the energy-dependent density of states, we determined the atomic structure and bonding of the MTB through simultaneous high-resolution non-contact atomic force microscopy. Density functional theory calculations based on the observed structure reproduced both the gap opening and the spatially resolved density of states.

Identification and Functional Characterization of R3 MYB Transcription Factor Genes in Soybean

Shucai Wang,Jinsong Pang,Hongwei Xun,Zhibing Zhang,Yunxiao Zhou,Xueyan Qian,Yingshan Dong,Xianzhong Feng,Bao Liu 한국식물학회 2018 Journal of Plant Biology Vol.61 No.2

In Arabidopsis, trichome formation is regulatedby a MYB-bHLH-WD40 (MBW) transcriptional activatorcomplex, which can activate the expression of GLABRA2(GL2) and R3 MYB genes. GL2 is required for trichomeformation, whereas R3 MYBs inhibit trichome formation byblocking the formation of the MBW complex, thus inhibitingthe expression of GL2. By using the amino acid sequence of theArabidopsis R3 MYB transcription factor TRICHOMELESS1(TCL1) to BLAST the soybean (Glycine max) protein database,we found that there are a total of six R3 MYB genes insoybean, namely Glycine max TRICHOMELESS1 through 6(GmTCL1-GmTCL6). By generating transgenic plants, wefound that trichome formation in soybean plants overexpressingeach of the GmTCLs remained largely unchanged, and theexpression of putative GL1 and GL2 genes in the transgenicplants was unaffected. However, all the GmTCLs interactedwith GLABRA3 (GL3) in transfected Arabidopsis protoplasts,expression each of the GmTCLs in Arabidopsis inhibitedtrichome formation, and the expression levels of GL1 andGL2 were greatly reduced in the Arabidopsis transgenicplants. Moreover, phenotypic complementary analysis showedthat GmTCL1 is functionally equivalent to TCL1. Takentogether, these results suggest that GmTCLs are functionalR3 MYBs, however, they do not regulate trichome formationin soybean.