Bacillus sp. LJ-25가 생성하는 extracellular chitinase의 정제 및 특성

주동식,조덕제,이정석,조만기,이응호 동서대학교부설연구소 1997 연구소 논문집 Vol.2 No.-

The extracellular chitinase from Bacillus sp. LJ-25 was pruified Sephadex G-100 gel filtration, DEAE-Cellulose and CM-Spephadex C25 column chromatography. The pruified chitinase showed a single band on disc and SDS polyacrylamide gel electrophoresis and the molecular weight was estimated to be about 50KDa. The Optimum pH and temperature for the activity of the purified extracellular chitinase were 7.5 and 35℃, respectively. The activity of chitinase was strongly inhibited by metal ions such as Zn²+, Ba²+, Co²+, Mn²+ and Cu²+. The purified chitinase did not hydrolyze N,N??-diacetylchitobiose, N,N, N'-triacetylchitotriose and p-nitrophenol-N-acetyl-β-D-glucosaminide, which are known to be the substrates of exo-type chitinase. The products in hydolysis of colloidal chitin with the chitinase were N-acetylglucosamine, N,N'-diacetylchitobiose and N,N,N'-triacetylchitotriose.

제어모드와 기준색 조정에 따른 CRT 모니터 색재현 알고리즘

이응주 동명정보대학교 2000 東明情報大學校論文集 Vol.3 No.-

When the user watches monitor under a certain conditions, the color is distorted due to monitor characteristic, spectral distribution of monitor, reference color and control mode of monitor. From these distortion factors, monitor colors can be seen differently under different conditions. And also, resulting colors of monitor are somewhat different under the kinds of same monitors. However, human visual system has color constancy with which the object color can be perceived constantly. A fundamental problem with any color monitor display system is the need to render correct colors under a variety of conditions as mentioned aboves. In order to predict the color errors, the tri-stimulus values must be computed under the reference color. The proposed method will compensate the resulting color errors and provide a natural color to the user.

아파트의 固體振動 低減對策에 관한 硏究

이주형,오제응 漢陽大學校 環境科學硏究所 1988 環境科學論文集 Vol.9 No.-

오늘날의 住居空間은 生活文化가 發達하고 變化함에 따라 經濟的이고 便利하여 졌으나, 騷音環境側面에서 불 때 적합하지 않은 문제점도 發生하게 되었다. 즉 騷音環境에 대한 要求가 더욱 세심해지고 住居空間의 構造變化에 따라 Wall Slab 아파트에서 structure-borne sound와 vibration이 심각한 문제로 제기되었다. 하지만 이에 대한 규제나 基準이 없는 실정이어서 生活文化發達에 부응하는 住居環境의 達成이 시급하다. 本 硏究에서는 Wall Slab구조 아파트에 대한 structure-borne sound와 vibration의 실상을 파악하기 위해서 "建築物의 現場에 있어서의 바닥 충격레벨의 測定方法"에 의해 소음과 진동을 측정하였다. 이에 대한 結論은 1) Wall Slab 와 R.C조를 비교한 결과 500㎐이하에서 약 10㏈의 차이를 보이면서 R.C조가 충격음과 진동에서 좋았다. 2) structure-borne sound와 vibration를 저감시키기 위해 Life Slab의 채택이 필요할 뿐만 아니라, 50~65m/m정도에 Life Slab는 가장 효율적인 節緣値를 나타냈다. The purpose of this study is to be calculated the problems of the Structure-bone sound and vibration to a apartment mode wall slab type and to be made the counterplan of noise reduction Actually based on respond to a resident, Structure-bone sound and vibration is measured with "Method for field measurement of floor impact sound level" The result of this study figures out: 1) The apartment mode reinforce concrete compared to the apartment made wall-siab has a good condition to a Structure-bone sound and vibration, taken the 10㏈ difference under the 500㎐. 2) Needed life-slab to apartment construction for the noise reduction of a apartment Structure-bone sound and vibration, having efficiently the 50~65m/m of life-slab increasing the degree of strength with a interior power wall within 5.Om of span in living space.

키틴 분해균 Bacillus sp. LJ-25의 분리 및 효소생산 최적화

이정석,주동식,조만기,이응호 동서대학교부설연구소 1997 연구소 논문집 Vol.2 No.-

In order to produce functional chitin oligosaccharides, a chitinolytic becterium was newly screened from the viscera of Korean bony fish, and identified as Bacillus sp. LJ-25. For the production of chitinolytic enzyme, 1.0% nutrient broth and 0.3% colloical chitin was used as nitrogen and carbon source, respectively. The optimal temperature, initial pH and conentration of NaCl for the production of chitinase from Bacillus sp. LJ-25 were 30℃, 6.5-7.0 and 1.0%, rspectively. Chitinolytic activity of Bacillus sp. LJ-25 was increased until the incubation of 168 hours, but after 168 hours, the activity was decreased.

마산 수출자유지역공단 근로자들에 대한 사회의학적 조사연구

이영호,정석주,이수일,김응남,맹광호 가톨릭대학산업의학센타 산업의학연구소 1977 韓國의 産業醫學 Vol.16 No.3

Followings are major findings obtained from a study on the socio-medical studus of the manu-facturing workers in the Masan Free Export Zone. This study was planned to find out socio-medical conditions of the workers which seemed to be essential to the better management of the health of the workers and the working environments. For this study, 6978 workers who are equivalent to 26.9% of total workers in Masan Free Export Zone as of the end of 1976 were sampled and they were asked to answer to the pretested questionnaire. 1) General characteristics; (1) 83.9% of sampled workers were female. (2) 84.9% of workers were less than 25 years of age and female workers who entered in this age group were 93.6%. (3) 91.9% of workers had more than junior high school education and those who finished only junior high school occupied the majority with the proportion of 67.7%. (4) 897.1% of workers were un-married and the proportion for the female was 93.2% (5) Majority (89.6%) were from Yongnam area and 5.2% of workers were from Honam area. (6) 49.1% of workers were living separately from their families and 76.9% of them were self-cooking. 2) Working conditions and working history; (1) 78.1% of workers were receiving from 20,000 won 40,000won as their monthly salaries and those who were receiving from 40,000 won to 60,000 won were the next (12.5%). (2) 34.4% of workers had been working in the present plant for from one to three years and those who had been working for less than one year were 31.9%. (3) Most of workers (91.2%) were working 8 hours a day but 25.3% of precision optical instruments manufacturing workers were working about 10 hours a day. (4) Only 6.2% of workers ever changed their working places during the past one year. 89.0% of them changed once and 9.0%, twice. Male workers changed their working places more often (8.7%) than female workers (5.7%). 3) Present and past history of illness; (1) 6.7% of workers had the sick among their family members and cardiovascular diseases were most common (47.8%) (2) Those who had experiences of sickness lasted more than one week were 7.1% and those who had had operations were 3.3%. (3) 42.0% of workers were complainning at least one physical symptom. Those who were complainning "tingling pain on eye ball" were the most (20.5%), and headache, and dizziness were both the next (16.3%). 4) Smoking, drinking habits and drug dependency; (1) Among all workers, 11.7% (male, 69.5%; female, 0.4%) were smoking and 13.1%(male, 71.9%; female, 2.3%) were drinking alchole. Those who were using drug customarily were only 2.3% and the drugs they were using were aspirin (35.0%), antibiotics (12.5%) and others. 5) Present physical conditions found at the periodic physical examinations; (1) Mean height of male workers was 168.3cm and that of female workers was 158.1cm. Mean body weight of male workers was 60.7kg and that of female workers was 51.3kg. (2) Those who had anormal vision were 10.1% and the frequency of self-awared hearing loss was 4.5%.If the visual power of one or both eyes was less than 0.6, we considered this as and abnormal vision. (3) Those who were informed by the physician that they had hypertension were 8.4% and those whe were informed that they had tuberculosis lesion on the chest were only 0.4%.

Graphical Analysis and Determination of Optimum Mixing Ratio of Bulking Agent for Composting of Food Waste

황은주,이주헌 대구대학교 환경기술연구소 2004 미래환경기술 Vol.1 No.1

Composting of food waste was studied in a controlled batch reactor with the addition of cooked rice as a biodegradable carbon source to investigate the effect of carbon to nitrogen (C/N) ratio control on composting. And, composting of bulking agents such as sawdust and wheat straw were tested with the addition of ammonium sulfate as a nitrogen source. As expected, biodegradation of the food waste having low C/N ratio was improved to some extent when foreign carbon was added. But, bulking agents used in this study exerted themselves slightly biodegradable carbon potential, indicating that the estimation of the dose of additional carbon considering desirable C/N ratio was not reasonable when lignocellulosic bulking material was added to food waste. Also, as another parameter for the estimation of bulking agent addition, the optimum moisture contents at different mixing ratios of bulking agent were evaluated. As a result, it was found that the optimum moisture content increased with sawdust addition meaning the C/N ratio increment. At these points, it was suggested that 78g sawdust / 100g food waste be mixed to make C/N ratio to 25 and moisture content to 56% for effective composting of the food waste studied in this paper.

카라기난 분해효소 생산균의 분리, 동정, 및 효소생산 최적 조건

양승택,주동식,박중제,이정석,김명식,이응호 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.6

해양 동식물, 토양 등으로부터 분리한 카라기난 분해능이 확인되었던 80여 균중에서 환원당 생성능이 가장 높았던 strain no.43 균주를 최종 시험균주로 선택하여 이 균주의 최적 효소 생산 조건을 실험하였다. 질소원으로는 nutrient broth 0.7% 농도가 가장 적절한 조건이었고, 탄소원의 농도는 카라기난 0.2%가 가장 높은 효소활성을 나타내었다. NaCl 농도는 1.5%, pH, 온도는 각각 7.0, 30℃로 이 조건에서 96시간 배양하는 것이 가장 적절한 효소 생산 조건이었음이 확인되었다. 각종 생화학 실험을 통해 동정을 행한 결과 그람음성 간균으로 oxidase, catalase 생성의 호기성균, indole 비생성, proline, arginine, serine, citrate, lactate 이용성, gelatin 액화, glucose, fructose, maltose 비이용, mannitol 이용 등의 특성으로부터 Pseudomonas alcaligenes로 동정할 수 있었고 Pseudomonas alcaligenes JCL-43으로 명명하였다. The 80 strains which produce carrageenan degrading enzyme were isolated from soils, mud, seaweed, marine moluscus and echonodermata samples. Among them, one isolated strain, which showed the highest activity to produced carrageenan degrading enzyme, was used for this study. The isolated strain was identified as Pseudomonas alcaligenes through its morphological, biochemical, and physiological characteristics. The best conditions for enzyme production were 0.7% nutrient broth and 0.2% carrageenan as nitrogen and carbon source, respectively. The optimal pH, NaCl, temperature and culture time for carrageenan degrading enzyme were 7.0, 1.5%, 30* and 96hrs, respectively.

장기간의 Cyclosporine 유지 요법으로 신기능을 유지 중인 FSGS 2예

권순길,김상현,김수희,박은주,이은경,박수길 대한신장학회 2004 대한신장학회잡지 Vol.23 No.1

Amiodarone에 의한 각막색소침착증 1예

이승진,박창규,김현철,김응주,김진원,이진수,임형준,안정천,이은미,서홍석,심완주,오동주,노영무 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.8

Annual measured performance of building-integrated solar energy systems in demonstration low-energy solar house

Lee, Kyoung-ho,Lee, Jin-kook,Yoon, Eung-sang,Joo, Moon-chang,Lee, Soon-myoung,Baek, Nam-choon American Institute of Physics 2014 Journal of renewable and sustainable energy Vol.6 No.4