http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
EUN JUNG LEE,Soyoung Shin,Jin-Kyoung Kim,Eun-Rhan Woo,김양미 대한화학회 2012 Bulletin of the Korean Chemical Society Vol.33 No.9
Amentoflavone is naturally occurring bioflavonoid that is found in a number of plants. In this paper, the antiinflammatory activity of amentoflavone in LPS-stimulated macrophages and its mode of action were examined. Using LPS-stimulated RAW264.7 macrophage cells, we found that amentoflavone exerted antiinflammatory activities through inhibition of nitric oxide (NO) production and tumor necrosis factor (TNF)-α and macrophage inflammatory protein (MIP)-2 secretion. Amentoflavone (1.0-20 μM) gradually inhibited nitrite production without cytotoxicity. Amentoflavone (1.0 and 10 μM) effectively suppressed both TNF-α and MIP-2 cytokine release from LPS-stimulated RAW264.7 cells. The expression of mIL-1β and mMIP-2 cytokine mRNAs was completely inhibited while expression of mMIP-1 was effectively suppressed and mTNF-α expression was slightly inhibited by 10 μM amentoflavone. We also demonstrated that the innate immune response to amentoflavone involves the toll-like receptor (TLR) and mitogen-activated protein kinase (MAPK) pathways. LPS-induced upregulation of p38 MAPK phosphorylation was significantly reduced by 10 μM amentoflavone. These results suggest that amentoflavone exhibits effective anti-inflammatory activities through regulation of TLR4 and phosphorylation of p38 MAPKs.
Styraxjaponoside A and B, Antifungal Lignan Glycosides Isolated from Styrax japonica S. et Z.
( Eun Rhan Woo ),( Dong Gun Lee ),( Jae Yong Cho ),( Mi Ran Kim ),( Ca Na Park ),( Bo Mi Hwang ),( In Sok Hwang ) 한국응용약물학회 2010 Biomolecules & Therapeutics(구 응용약물학회지) Vol.18 No.4
The antifungal effects and action mechanisms of styraxjaponoside A and B were investigated. Devoid of hemolytic effect, the compounds had significant effect against several human pathogenic fungal strains, with energy-independent manners. To understand the action mechanisms of the compounds, the flow cytometric analysis plotting the forward scatter and the side scatter, DiBAC4(3) staining and DPH fluorescence analysis were conducted. The results indicated that the actions of the compounds were dependent upon the membrane-active mechanisms. The present study suggests that styraxjaponoside A and B exert their antimicrobial effects via membrane-disruptive mechanisms.
마타리 뿌리로부터 분리한 Lignan 화합물의 IL-6 저해활성
최은진(Eun Jin Choi),김청룡(Qinglong Jin),신지은(Ji Eun Shin),김현규(Hyun-Gyu Kim),김정진(Jung Jin Kim),우은란(Eun-Rhan Woo) 대한약학회 2009 약학회지 Vol.53 No.4
In an ongoing investigation into anti-inflammatory compounds from natural products, the CH2Cl2soluble fraction of Patrinia scabiosaefolia F. (Valerianaceae) was found to inhibit IL-6 production in TNF-α stimulated MG-63 cell line. By means of a bioassay-directed chromatographic separation technique, lappaol E (1), and nortrachelogenin (2) were isolated. These compounds have been isolated from this plant for the first time. Compounds 1~2 showed potent antioxidative activity using NBT superoxide scavenging assay. Moreover, compound 1 decreased IL-6 production in TNF-α stimulated MG-63 cell line.
Virus-cell Fusion Inhibitory Activity for the Polysaccharides from Various Korean Edible Clams
Woo, Eun-Rhan,Kim, Wan-Seok,Kim, Yeong-Shik The Pharmaceutical Society of Korea 2001 Archives of Pharmacal Research Vol.24 No.6
In order to find potent virus-cell fusion inhibitory components from Korean edible clams, thirteen prepared polysaccharides were introduced to syncytia formation inhibition assay, which is based on the interaction between the HIV-1 envelope protein gp 120/41 and the cellular membutane protein CD4 of T lymphocytes. Among them, Meretrix petechialis showed a potent viruscell fusion inhibitory activity. Fusion index (F1) and percent (%) fusion inhibition of the polysaccharide of this clam were $0.21{\pm}0.02$, and $67.52{\pm}4.09$ at 100781m1, respectively. It exhibited almost equivalent virus-cell fusion inhibitory activity to that of dextran sulfate which was used as a standard control.
Antioxidative Constituents from Lycopus lucidus
Woo, Eun-Rhan,Piao, Mei-Shan The Pharmaceutical Society of Korea 2004 Archives of Pharmacal Research Vol.27 No.2
Three phenolic compounds, rosmarinic acid (1), methyl rosmarinate (2), ethyl rosmarinate (3), and two flavonoids, luteolin (4), luteolin-7-O-$\beta$-D-glucuronide methyl ester (5) were isolated from the aerial part of Lycopus lucidus (Labiatae). Their structures were determined by chemical and spectral analysis. Compounds 1-5 exhibited potent antioxidative activity on the NBT superoxide scavenging assay. The $IC_{50}$ values for compounds 1-5 were 2.59, 1.42, 0.78, 2.83, and 3.05 $\mu\textrm{g}$/mL respectively. In addition, five compounds were isolated from this plant for the first time.
Ji Eun Shin,Eun-Rhan Woo,Eun Jin Choi,Qinglong Jin,Hong-Guang Jin 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.3
Six chalcone compounds, 2',4',4-trihydroxy-3'-[2-hydroxy-7-methyl-3-methylene-6-octaenyl]chalcone (1), 2',4',4-trihydroxy-3'-geranylchalcone (2), 2',4',4-trihydroxy-3'-[6-hydroxy-3,7-dimethyl-2,7-octadienyl]chalcone (3), 2',4-dihydroxy-4'-methoxy-3'-[2-hydroperoxy-3-methyl-3-butenyl]chalcone (4), 2',4-dihydroxy-4'-methoxy-3'-geranylchalcone (5), and 2',4-dihydroxy-4'-methoxy-3'-[3-methyl-3-butenyl]chalcone (6) were isolated from the leaves of Angelica keiskei K (Umbelliferae). The structure of each isolated compound was determined using spectroscopic methods. Among the isolates, compounds 1-3 appeared to have potent inhibitory activity of IL-6production in TNF-α-stimulated MG-63 cell, while compounds 4-6 did not. The distinct structural difference between compounds 1-3 and 4-6 was the presence of C-4' hydroxyl group in the chalcone moiety. Our results imply that the inhibitory activity of IL-6 production in TNF-α-stimulated MG-63 cell may be affected by the presence of C-4' hydroxyl group in the chalcone moiety.
Lee, Eun-Jung,Shin, So-Young,Lee, Jee-Young,Lee, So-Jung,Kim, Jin-Kyoung,Yoon, Do-Young,Woo, Eun-Rhan,Kim, Yang-Mee Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.7
Human peroxisome proliferator-activated receptor gamma ($hPPAR{\gamma}$) has been implicated in numerous pathologies, including obesity, diabetes, and cancer. Previously, we verified that amentoflavone is an activator of $hPPAR{\gamma}$ and probed the molecular basis of its action. In this study, we investigated the mechanism of action of amentoflavone in cancer cells and demonstrated that amentoflavone showed strong cytotoxicity against MCF-7 and HeLa cancer cell lines. We showed that $hPPAR{\gamma}$ expression in MCF-7 and HeLa cells is specifically stimulated by amentoflavone, and suggested that amentoflavone-induced cytotoxic activities are mediated by activation of $hPPAR{\gamma}$ in these two cancer cell lines. Moreover, amentoflavone increased PTEN levels in these two cancer cell lines, indicating that the cytotoxic activities of amentoflavone are mediated by increasing of PTEN expression levels due to $hPPAR{\gamma}$ activation.
Inhibition of Nuclear Factor-κB Activation by 2′,8″-Biapigenin
Woo, Eun-Rhan,Pokharel, Yuba Raj,Yang, Jin Won,Lee, Song Yi,Kang, Keon Wook Pharmaceutical Society of Japan 2006 Biological & pharmaceutical bulletin Vol.29 No.5
<P>Inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) play a key role in the inflammatory processes. Improper overproduction of NO and prostaglandins by both enzymes are also believed to be involved in the pathogenesis of certain human cancers. Crude extracts of <I>Selaginella tamariscina</I> are used as an oriental medicine, which has been reported to inhibit the production of proinflammatory cytokines and cause cell cycle arrest. We isolated 2′,8″-biapigenin from <I>S. tamariscina</I> and investigated whether it modulates iNOS and COX-2 expressions in Raw264.7 macrophages stimulated with lipopolysaccharide (LPS). We found that 2′,8″-biapigenin blocked the transactivations of iNOS and COX-2 genes <I>via</I> the inactivation of nuclear factor-κB by preventing the nuclear translocation of p65. Hence, it may be possible to develop <I>S. tamariscina</I> extracts or 2′,8″-biapigenin as a useful agent for cancer chemoprevention or for the treatment of inflammatory diseases.</P>
Structural Implication in Cytotoxic Effects of Sterols from Sellaginella tamariscina
Roh, Eun-Mi,Jin, Qing-Long,Jin, Hong-Guang,Shin, Ji-Eun,Choi, Eun-Jin,Moon, Young-Hee,Woo, Eun-Rhan 대한약학회 2010 Archives of Pharmacal Research Vol.33 No.9
A bioassay-guided fractionation of the $CH_2Cl_2$ extract of Selaginella tamariscina yielded six sterols 1-6 such as (4${\alpha}$, 5${\alpha}$)-4, 14-dimethylcholest-8-en-3-one (1), ergosta-4, 6, 8(14), 22-tetraene-3-one (2), ergosterol endoperoxide (3), 7${\beta}$-hydroxycholesterol (4), 7${\beta}$-hydroxysitosterol (5), and 7${\alpha}$-hydroxysitosterol (6). The structures of isolated compounds were determined using spectroscopic methods. Among these isolates, compounds 2-5 showed potent cytotoxicity against five human tumor cells, while compounds 1 and 6 did not. In the case of compounds 1 and 2, 3-oxo sterol derivatives, compound 1 was inactive, but compound 2 showed potent cytotoxicity. In addition, compound 5 exhibited potent cytotxicity, but, compound 6 which is the 7-epimer of compound 5 was weakly active against tumor cell lines. Therefore, in the case of oxysterol derivatives, the cytotoxicity appeared to be affected by the structural differences, i.e. the configuration of hydroxyl group and the number of conjugated double bond. Taken all together, the present study isolated six sterols from S. tamariscina for the first time based on a bioassay-guided fractionation and indicated that isolated oxysterols could exhibit the cytotoxic effects against tumor cells, suggesting that S. tamariscina might be a promising candidate for the development of anticancer agents.