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RISS 인기검색어
- 검색키워드
- 저자 : EnochY.Park (검색결과 3 건)
- 무료
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- 유료
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Empirical Evaluation of Cellulase on Enzymatic Hydrolysis of Waste Office Paper
EnochY.Park,Yuko Ikeda,Naoyuki Okuda 한국생물공학회 2002 Biotechnology and Bioprocess Engineering Vol.7 No.5
Enzymatic hydrolysis of waste office paper was evaluated using three commercial cellulases, Acremonium cellulase, Meicelase, and Cellulosin T2. Varying the enzyme loading from 1 to 10% (w/w) conversion of waste office paper to reducing sugar was investigated. The conversion increased with the increase in the enzyme loading: in the case of enzyme loading of 10% (w/w), Acremonium cellulase yielded 79% conversion of waste office paper, which was 17% higher compared to Meicelase, 13% higher than that of Cellulosin T2. Empirical model for the conversion (%) of waste office paper to reducing sugar (x) was derived from experimental results as follow, x = kEm t(aE+b) where k, m, a, and b denote empirical constants. E indicates initial enzyme concentration.
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The use of nanocrystal quantum dot as fluorophore reporters in molecular beacon-based assays
Adegoke Oluwasesan,EnochY.Park 나노기술연구협의회 2016 Nano Convergence Vol.3 No.32
The utilization of molecular beacon (MB) biosensor probes to detect nucleic acid targets has received enormous interest within the scientific community. This interest has been stimulated by the operational qualities of MB-based probes with respect to their unique sensitivity and specificity. The design of MB biosensors entails not only optimizing the sequence of the loop to hybridize with the nucleic acid target or optimization of the length of the stem to tune the sensitivity but also the selection of the appropriate fluorophore reporter to generate the signal transduction read-out upon hybridization of the probe with the target sequence. Traditional organic fluorescent dyes are mostly used for signal reporting in MB assays but their optical properties in comparison to semiconductor fluorescent quantum dot (Qdot) nanocrystals are at a disadvantage. This review highlights the progress made in exploiting Qdot as fluorophore reporters in MB-based assays with the aim of instigating further development in the field of Qdot-MB technology. The development reported to date indicates that unparalleled fluorescence signal reporting in MB-based assays can be achieved using well-constructed Qdot fluorophores.
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Production of Arachidonic Acid by Mortierella Fungi
Kenichi Higashiyama,Shigeaki Fujikawa,EnochY.Park,Sakayu Shimizu 한국생물공학회 2002 Biotechnology and Bioprocess Engineering Vol.7 No.5
The growing interest in the application of arachidonic acid (ARA) in various fields of health and dietary requirements has elicited much attention on the industrial production of ARA-containing oil by the cultivation of Mortierella fungi. For the industrial production of ARA, various studies, such as isolation of a high-potential strain and optimization of culture conditions, have been conducted. Studies including the investigation of morphology are important because ARA is accumulated in the mycelia, and thus cultivation with high biomass concentration is essential for obtaining a high ARA yield. Combining the results derived from various studies, a high ARA yield was attained in an industrial fermentor. These ARA production techniques are applicable to the production of other polyunsaturated fatty acids (PUFAs), and will contribute to the improvement of fermentation technology especially in the field of fungal cultivation.
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