Development of HPV16 mouse and dog models for more accurate prediction of human vaccine efficacy

Emmanuelle Totain,Loïc Lindner,Nicolas Martin,Yolande Misseri,Alexandra Iché,Marie-Christine Birling,Tania Sorg,Yann Herault,Alain Bousquet-Melou,Pascale Bouillé,Christine Duthoit,Guillaume Pavlovic,S 한국실험동물학회 2023 Laboratory Animal Research Vol.39 No.2

Background: Animal models are essential to understand the physiopathology of human diseases but also to evaluate new therapies. However, for several diseases there is no appropriate animal model, which complicates the development of effective therapies. HPV infections, responsible for carcinoma cancers, are among these. So far, the lack of relevant animal models has hampered the development of therapeutic vaccines. In this study, we used a candidate therapeutic vaccine named C216, similar to the ProCervix candidate therapeutic vaccine, to validate new mouse and dog HPV preclinical models. ProCervix has shown promising results with classical subcutaneous murine TC-1 cell tumor isografts but has failed in a phase II study. Results: We first generated E7/HPV16 syngeneic transgenic mice in which the expression of the E7 antigen could be switched on through the use of Cre–lox recombination. Non-integrative LentiFlash® viral particles were used to locally deliver Cre mRNA, resulting in E7/HPV16 expression and GFP reporter fluorescence. The expression of E7/HPV16 was monitored by in vivo fluorescence using Cellvizio imaging and by local mRNA expression quantification. In the experimental conditions used, we observed no differences in E7 expression between C216 vaccinated and control groups. To mimic the MHC diversity of humans, E7/HPV16 transgenes were locally delivered by injection of lentiviral particles in the muscle of dogs. Vaccination with C216, tested with two different adjuvants, induced a strong immune response in dogs. However, we detected no relationship between the level of cellular response against E7/HPV16 and the elimination of E7-expressing cells, either by fluorescence or by RT-ddPCR analysis. Conclusions: In this study, we have developed two animal models, with a genetic design that is easily transposable to different antigens, to validate the efficacy of candidate vaccines. Our results indicate that, despite being immunogenic, the C216 candidate vaccine did not induce a sufficiently strong immune response to eliminate infected cells. Our results are in line with the failure of the ProCervix vaccine that was observed at the end of the phase II clinical trial, reinforcing the relevance of appropriate animal models.

Implications of a two-step procedure in surgical management of patients with early-stage endometrioid endometrial cancer

Emmanuelle Arsène,Géraldine Bleu,Benjamin Merlot,Loïc Boulanger,Denis Vinatier,Olivier Kerdraon,Pierre Collinet 대한부인종양학회 2015 Journal of Gynecologic Oncology Vol.26 No.2

Objective: Since European Society for Medical Oncology (ESMO) recommendations and French guidelines, pelvic lymphadenectomyshould not be systematically performed for women with early-stage endometrioid endometrial cancer (EEC) preoperativelyassessed at presumed low- or intermediate-risk. The aim of our study was to evaluate the change of our surgical practices afterESMO recommendations, and to evaluate the rate and morbidity of second surgical procedure in case of understaging after thefirst surgery. Methods: This retrospective single-center study included women with EEC preoperatively assessed at presumed low- orintermediate-risk who had surgery between 2006 and 2013. Two periods were defined the times before and after ESMOrecommendations. Demographics characteristics, surgical management, operative morbidity, and rate of understaging werecompared. The rate of second surgical procedure required for lymph node resection during the second period and its morbiditywere also studied. Results: Sixty-one and sixty-two patients were operated for EEC preoperatively assessed at presumed low-or intermediate-riskbefore and after ESMO recommendations, respectively. Although immediate pelvic lymphadenectomy was performed morefrequently during the first period than the second period (88.5% vs. 19.4%; p<0.001), the rate of postoperative risk-elevating orupstaging were comparable between the two periods (31.1% vs. 27.4%; p=0.71). Among the patients requiring second surgicalprocedure during the second period (21.0%), 30.8% did not undergo the second surgery due to their comorbidity or old age. For the patients who underwent second surgical procedure, mean operative time of the second procedure was 246.1±117.8minutes. Third operation was required in 33.3% of them because of postoperative complications. Conclusion: Since ESMO recommendations, second surgical procedure for lymph node resection is often required for womenwith EEC presumed at low- or intermediate-risk. This reoperation is not always performed due to age/comorbidity of thepatients, and presents a significant morbidity.

ANISOTROPIC ASSEMBLY OF COLLOIDAL NANOPARTICLES: EXPLOITING SUBSTRATE CRYSTALLINITY

EMMANUELLE PAULIAC-VAUJOUR,JAMES A. HAYTON,PHILIP J. MORIARTY 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2007 NANO Vol.2 No.6

We show that the crystal structure of a substrate can be exploited to drive the anisotropic assembly of colloidal nanoparticles. Pentanethiol-passivated Au particles of ~ 2 nm diameter deposited from toluene onto hydrogen-passivated Si(111) surfaces form linear assemblies (rods) with a narrow width distribution. The rod orientations mirror the substrate symmetry, with a high degree of alignment along principal crystallographic axes of the Si(111) surface. There is a strong preference for anisotropic growth with rod widths substantially more tightly distributed than lengths. Entropic trapping of nanoparticles provides a plausible explanation for the formation of the anisotropic assemblies we observe.

Association between Nutrition Behavior and Colorectal Cancer Diet Recommendation

Laguerre Emmanuelle,Matthews Tracy 대한암예방학회 2022 Journal of cancer prevention Vol.27 No.2

The incidence of colorectal cancer has considerably increased worldwide, particularly among adults aged 50 and older. Despite numerous nutrition initiatives, colorectal cancer (CRC) remains a public health burden that affects younger adults in the United States. Understanding the potential factors contributing to non-adherence to nutrition recommendations can be helpful to develop effective nutrition initiatives to prevent CRC. This study aimed to determine differences in nutrition knowledge, attitudes, and beliefs (KAB); examine their associations on diet characteristics and weight status; and identify factors influencing eating patterns among ethnically diverse populations at risk for CRC and living in urban areas. The study used a quantitative descriptive and correlational research design in which data were collected through an online cross-sectional survey. A total of 377 participants responded to the survey. The study revealed a few significant differences in KAB levels between males and females. KAB levels were not associated with weight status but with meat recommendations among overweight or obese males. Ultimately, the study identified perceived barriers and facilitators as factors influencing participants’ diets. Differences in KAB among males and females were inconsistent with the diet characteristics and weight status variables. This study suggests acknowledging these differences and inconsistencies when designing nutrition initiatives focusing on colorectal cancer prevention

컬러 믹싱 외

엠마누엘 모레악스 아키텍쳐 + 디자인(emmanuelle moureaux architectu),다이스케 시마(사진),델롯 에디션즈(Derlot editions),플로리안 그로옌(사진) 현대건축사 2017 (월간)Maru Vol.- No.179

컬러 믹싱 외

엠마누엘 모레악스 아키텍쳐 + 디자인(emmanuelle moureaux architectu),다이스케 시마(사진),델롯 에디션즈(Derlot editions),플로리안 그로옌(사진) 현대건축사 2017 마루 Vol.- No.179

EU에서 새롭게 제기되는 사회심리학적 위험

Malgorzata Milczarek,Emmanuelle Brun 한국노동연구원 2011 국제노동브리프 Vol.9 No.1

Strategic Interactions on structural Reforms Between Intergrated Economies: What Lessons for the EU?

Etienne Lehmann,Emmanuelle Taugourdeau 세종대학교 경제통합연구소 2004 Journal of Economic Integration Vol.19 No.1

We show that the domestic output increases with a foreign country's structural reform and decreases with foreign country’s fiscal policy. Hence, when governments act non-cooperatively, they implement too low structural reforms and too much fiscal policies. We show that the domestic output increases with a foreign country's structural reform and decreases with foreign country’s fiscal policy. Hence, when governments act non-cooperatively, they implement too low structural reforms and too much fiscal policies.

IT’S TOUGH AT THE TOP. ETHICAL COMPETENCES OF NEW LUXURY FASHION ACTORS

Colette Depeyre,Emmanuelle Rigaud,Fabien Seraidarian 글로벌지식마케팅경영학회 2017 Global Fashion Management Conference Vol.2017 No.07

The willingness to actively contribute to more sustainability bares important challenges for managers who must take strategic decisions. The literature about corporate social responsibility has developed in many directions, with often a divide in focus, between society (Acquier & Aggeri, 2015) or corporate sustainability and responsibility (Bansal & Song, 2017). The paper sits at the intersection of these fields by focusing on the question of ethical dilemmas. The objective is to approach the question of ethics in context, through the trade-offs that managers are facing when taking strategic decisions to build sustainable business models, to discuss the contours of the ethical competences at work. The paper focuses on a specific context, the one of successful pure players of sustainability in the luxury fashion industry. It means first that we leave aside the question of organizational change to analyze the core dilemmas that even new actors that start from scratch must face. These pure players could also be named “social entrepreneurs” (Elkington & Hartigan, 2008) or “elegant disrupters” (Bendell & Thomas, 2013). Second, by focusing on luxury fashion we locate our analysis in an industry where the nature of the activity is both a source of salient controversies and of exemplary practices (Kapferer, 2010; Godart & Seong, 2014), two aspects that have the potential to make visible some dilemmas. We focus on a small number of cases of organizations that we selected per criteria of similarities and differences, to allow for an inductive in-depth comparative case study (Yin, 2009). The four organizations are: a producer of yak products, a fur recycling company, a fur auction house and a digital platform for emerging designers. The cases are similar in that each organization is a successful promoter of sustainable practices. The four cases however differ in that each puts at the core of the business model different types of sustainability issues and is based at a different level of the value chain (sourcing, fabrication, intermediation and market access). Also, the four organizations differ in the degree to which they aim to disrupt existing practices in the luxury fashion industry. Data were collected through a series of interviews and complementary press articles, websites and reports. Data analysis proceeds in two steps. We start by narrating each case through the voice of the organization, to describe and understand the rationale behind each business model. We then further our interpretation of the cases through a comparative analysis by systematically recording the tensions or contradictions. Each firm organizes the activity around key engaging choices that promote sustainability but do not lift some inbuilt dilemmas–labelled as: sustainability vs. luxury fashion, sustainability vs. sustainability, and sustainability today vs. tomorrow. The paper highlights how innovative players can openly discuss some dilemmas, approaching sustainability as a story they care about but also as a debate. Yet, the paper also points to the fact that some dilemmas may remain harder to reflect upon, especially the ones that can counter in the long-run the objectives that were at the base of the business. Some questions as the one of growth, in time and scale, might push far the reflexive ability but it is also where ethical competences might be the most needed.

Strategic Interactions on Structural Reforms Between Integrated Economies: What Lessons for the EU?

( Etienne Lehmann ),( Emmanuelle Taugourdeau ) 세종대학교 경제통합연구소 (구 세종대학교 국제경제연구소) 2004 Journal of Economic Integration Vol.19 No.1

We show that the domestic output increases with a foreign country`s structural reform and decreases with foreign country`s fiscal policy. Hence, when governments act non-cooperatively, they implement too low structural reforms and too much fiscal policies.