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- 저자 : Dwi Nur Happy Hariyono (검색결과 3 건)
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Dwi Nur Happy Hariyono,Dyah Maharani,조성현,Prabuddha Manjula,서동원,최누리,Jafendi Hasoloan Purba Sidadolog,이준헌 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.1
Objective: At least eight local duck breeds have been recognized and documented as national germplasm of Indonesia so far. It is necessary to genetically characterize the local duck breeds for aiding conservation and future improvement strategies. Thus, this study was carried out to assess genetic diversity and phylogenetic relationship of eight local duck populations of Indonesia using microsatellite markers. Methods: In total, 240 individuals (30 individuals each population) from Alabio (AL), Bayang (BY), Magelang (MG), Mojosari (MJ), Pegagan (PG), Pitalah (PT), Rambon (RM), and Turi (TR) duck populations were genotyped using 22 microsatellite markers. Results: The results showed a moderate level of genetic diversity among populations, with a total of 153 alleles detected over all loci and populations, ranging from 3 to 22 alleles per locus. Observed (Ho) and expected heterozygosity (He), as well as polymorphism information content over all loci and populations were 0.440, 0.566, and 0.513, respectively. Heterozygote deficiency in the overall populations (FIT = 0.237), was partly due to the heterozygote deficiency within populations (FIS = 0.114) and moderate level of genetic differentiation among populations (FST = 0.137). The most diverse population was MG (He = 0.545) and the least diverse population was AL (He = 0.368). The majority of populations were relatively in heterozygote deficiency (except AL), due to inbreeding. The genetic distances, phylogenetic trees, and principal coordinates analysis concluded that the populations can be grouped into two major clusters, resulting AL, MG, and MJ in one cluster separated from the remaining populations. Conclusion: The present study revealed a considerable genetic diversity of studied populations and thus, proper management strategies should be applied to preserve genetic diversity and prevent loss of alleles.
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Association of selected gene polymorphisms with thermotolerance traits in cattle - A review
Hariyono, Dwi Nur Happy,Prihandini, Peni Wahyu Asian Australasian Association of Animal Productio 2022 Animal Bioscience Vol.35 No.11
Thermal stress due to extreme changes in the thermal environment is a critical issue in cattle production. Many previous findings have shown a decrease in feed intake, milk yield, growth rate, and reproductive efficiency of cattle when subjected to thermal stress. Therefore, selecting thermo-tolerant animals is the primary goal of the efficiency of breeding programs to reduce those adverse impacts. The recent advances in molecular genetics have provided significant breeding advantages that allow the identification of molecular markers in both beef and dairy cattle breeding, including marker-assisted selection (MAS) as a tool in selecting superior thermo-tolerant animals. Single-nucleotide polymorphisms (SNPs), which can be detected by DNA sequencing, are desirable DNA markers for MAS due to their abundance in the genome's coding and non-coding regions. Many SNPs in some genes (e.g., HSP70, HSP90, HSF1, EIF2AK4, HSBP1, HSPB8, HSPB7, MYO1A, and ATP1A1) in various breeds of cattle have been analyzed to play key roles in many cellular activities during thermal stress and protecting cells against stress, making them potential candidate genes for molecular markers of thermotolerance. This review highlights the associations of SNPs within these genes with thermotolerance traits (e.g., blood biochemistry and physiological responses) and suggests their potential use as MAS in thermotolerant cattle breeding.
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Dyah Maharani,Simon P. Ginting,Simon Elieser,Andi Tarigan,I Gede Saputra Budisatria,Antonius,Dwi Nur Happy Hariyono,Aprilianna Putri Zahara Nafsina Luvita Sari 한국동물유전육종학회 2021 한국동물유전육종학회지 Vol.5 No.1
Calpastatin (CAST) gene has been considered as a candidate gene for meat quality traits in livestock animals. In this study, single nucleotide polymorphisms (SNPs) of the CAST gene were evaluated for determination of haplotype block pattern and linkage disequilibrium in 21 Boer × Kacang (Boerka) crossbred goats. Five SNPs: g.146C>A (SNP1), g.224A>G (SNP2), g.281G>A (SNP3), g.373C>T (SNP4) and g.431G>A (SNP5) were found in CAST gene (620 bp) using direct sequencing. Based on these SNPs, four haplotype block patterns (A, B, C and D) with different SNPs combination were performed. All blocks had high D’prime equal to 1.0. The highest LOD (1.75), r2 (1.00) and haplotype frequency (97.6%) were observed in block A (SNP1&2, SNP1&5 and SNP2&5). The lowest LOD (0.01) and r2 (0.0010) were found in block A (SNP1&4, SNP2&4 and SNP4&5). Block B, C and D had only 3, 2 and 1 SNPs combination, respectively, with a medium haplotype frequency (57.1%) each. Our study demonstrated that the SNPs and their haplotype combinations in the CAST gene could be useful for association studies with meat quality traits.
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