생물공학응용을 위한 졸-겔 기반 광학센서 및 양자점 프로브에 관한 연구

Hong, Dinh Duong 전남대학교 대학원 2007 국내박사

Enzyme glucose oxidase (GOD) was immobilized on the sol-gel films of 3-glycidoxypropyltrimethoxysilane (GPTMS) and its mixtures with methyltriethoxysilane (MTES) and 3-aminopropyltrimethoxysilane (APTMS) to evaluate the ability of these sol-gels for enzyme immobilization. The film containing GPTMS (20v/v%) and APTMS (5v/v%) was an excellent membrane for enzyme immobilization in term of high enzyme activity and stability. Ruthenium diphenylphenanthroline complex, a highly fluorescent and photostable dye, is embedded in several sol-gel films (GA1, GA2, GM1, GM2) to produce the oxygen sensing films. The films were prepared in situ on the bottom of the wells of the 96 well microtiter plate. The prepared plate was employed as micro bioreactor for Escherichia coli JM 109 cultivation. The oxygen consumed by E.coli was measured by the oxygen sensing membranes. All sensing membranes are sensitive to the change in oxygen concentration, the membrane used sol-gel GM2 (12.5 v/v% of GPTMS and 25v/v% of MTES) showed the highest ability. Based on the above results the optical planar biosensors for detection of glucose, lactate and tyramine using the microtiter plate were developed. The enzymes (glucose oxidase, lactate oxidase, tyramine oxidase) were immobilized on the sol-gel GPTMS and the sol-gel mixtures of GPTMS and MTES or GPTMS andAPTMS. The oxygen sensitive dye, ruthenium complex, was entrapped in sol-gel mixtures of GPTMS and MTES. The linear detection ranges of these sensing membranes were of 0.1-5.0 g/L for glucose, 7.77-90 mg/L for lactate and 6.3-100 mg/L for tyramine. The covalent binding of the enzymes in the sol-gel films preserved the high stability of the sensing membranes for more than 10 months. The pH indicator, fluoresceinamine isomer II, was immobilized in the solgel matrix made by two precursors, MTES and GPTMS to produce the optical pH sensor. The immobilization process was carried out in one simple step of the covalent binding between GPTMS and fluoresceinamine. The suitable combination of two precursors, GPTMS and MTES, was a favor support matrix of fluoresceinamine, and showed the high sensitivity with the extension of pH detection range up to 4-10. The linear range was from pH 6 to pH 9 (R² = 0.995). Optical temperature sensors were prepared using a fluorescent dye, rhodamine B, and different adsorbents, viz. silica gel and sol-gel. Silica gel (28-200 mesh) and a sol-gel consisting of a mixture of APTMS and GPTMS were used as the support matrices for the fluorescent dye. The linear detection ranges of the fiber optic temperature sensors were 10-95 ℃ and 0-60 ℃ when using silica gel and sol-gel respectively as the support materials. The planar optic temperature sensors showed high sensitivity in the temperature range of 25-40 ℃. The life time of the sensors was extended more than 3 months. CdSe/ZnS core/shell quantum dots (QDs) were synthesized with different emission wavelength 590-620nm. QDs (590nm) were conjugated with enzyme glucose oxidase (GOD) and horseradish peroxidase (HRP). The complex of GOD/HRP-conjugated QDs was used as quantum dot FRET-based probes in sensing glucose. The quenching of QD fluorescence was corresponding to glucose concentration. Linear detection range of using enzymes-conjugated QDs in glucose solutions was of 0-5 g/L (R = 0.992). Anti-insulin antibody was labeled by water soluble CdSe/ZnS QDs (605nm) and used for insulin detection. The conjugation of quantum dots and antibody was performed directly or indirectly through streptavidin and biotinylated second anti insulin antibody. The direct conjugation showed linear detection range of 0.2-8.0 μM, (R=0.988), whereas, the indirect conjugation indicated linear detection range of 0.2-10 μM (R=0.977) and signal clearly separated at insulin concentrations of 0.2-1.0 μM after 1.5 hours of initial measurements.

Cloning and Characterization of Valuable Genes of Chrysanthemum

Duong Huyen Trang The Graduate school of Dong-A university 2012 국내석사

국화 유래 유용 유전자 클로닝 및 기능 해석 Cloning and Characterization of Valuable Genes of Chrysanthemum 의생명과학과 Duong Huyen Trang 지 도 교 수 김 경 태 국화는 세계적으로 가장 중요한 절화와 분화식물중에 하나이다. 유전적, 분자적 기술과 함께 전통적인 육종은 꽃의 색과 크기 및 형태, 식물의 높이, 성장모양과 빛의 질에 대한 민감성을 통해 관상용 화훼작물로써의 가치 강화에 집중된다. 국화의 형질전환은 분류학상의 장벽을 넘은 유전자에 암호화된 특성의 도입을 허락함으로써 더 광범위하고 다양한 작물향상을 고려한다. Auxin response factors (ARFs)는 옥신 반응 유전자의 발현을 규제하는 전사인자이다. ARFs 집단은 ARF5와 ARF1는 배발생에, ARF7, ARF19, ARF10과 ARF16은 뿌리발달에, ARF2, ARF3, ARF6과 ARF8은 꽃발달에, 그리고 ARF1과 ARF2에 의한 노화에 관여하는 것으로 알려져 있다. 그러나 ARF18의 기능은 명확하지 않다. 그래서 ARF18의 기능을 연구하기 위해 애기장대와 국화로 애기장대 유래의 ARF18유전자를 옮겼다. 형질전환된 애기장대에서의 기관 내 세포크기는 증가했다. 그것은 AtARF18가 애기장대의 세포크기에 있어 영향을 준다는 것을 나타낸다. 형질전환된 국화에 6개의 형질전환라인을 가지고 있고 꽃에 대해 연구하였다. 우리는 ARF18 OX 8T와 ARF18 OX20T 2라인을 선택하였고 꽃잎의 길이를 측정하였다. 우리는 형질전환된 꽃이 야생형보다 꽃잎의 크기가 길다는 것을 알았다. 그러나 그것은 AtARF18이 꽃잎크기에 영향을 미친다고 말하기에는 충분하지 않다. 애기장대 유래의 FLOWERING LOCUS T (AtFT) 는 광주반응에 중요한 역할을 하며 AtFT 유전자의 돌연변이가 일어나면, 그 결과 유도적인 장일 조건에서 개화가 빨리 일어나는 것이 특징이다. 하지만 단일조건에서의 FT 유전자의 기능은 아직 알려지지 않았다. CmFT 유전자는 국화 λ phage cDNA 라이브러리로부터 CmFT 특정 프라이머를 사용하여 PCR을 수행하여 분리하였다. CmFT 유전자의 염기서열 분석 결과로 상동성을 비교한 결과, 애기장대와 국화 간의 상동성 유사관계가 72%로 나타났다. CmFT 유전자의 정확한 기능을 알고자 이 CmFT 유전자를 CaMV 35S 프로모터와 Super 프로모터를 가지는 pCAMBIA 3300에 클로닝 하였다. 구축된 CmFT 발현 시스템을 애기장대에 형질전환 시켰고 지금 그 형질전환체의 분석을 기다리고 있다. 이 분석을 토대로 CmFT 애기장대에서의 역할 규명을 한 다음, 국화로 형질전환을 할 것 이다. 주요어 : 애기장대, 국화, AtARF18, CmFT.

A quantification of inorganic mass partitioning to particle between low/high humidity and with/without seed particle and VOCs.

Duong Hoang Do Duong Hoang Do 2020 국내석사

Ammonia (NH3) and nitrogen oxide (NOx) in the atmosphere have critical impacts on the nitrogen cycle, ecosystem, and secondary organic aerosol formation. NH3 and NOx uptake occurs into aerosol liquid water (ALW), which is formed in inorganic-rich hygroscopic particles at a high relative humidity (RH), and this is an important process in East Asia. Therefore, the NH3 participation in NOx photochemistry of secondary aerosol formation, focusing on quantifying inorganic compounds, needs to be explored. In this study, we conduct photochemical reactions of particles in a smog chamber (6 m3) to monitor NH3 and NOx partitioning from the gas phase to wet aerosols, varying four different factors: relative humidity (RH), present of seed particle, present of volatile organic compound and effects of NOx concentration. Experiments are conducted separately to study the impact of those factor Atomizing solution of (NH4)2SO4 is introduced to the smog chamber as seed particles with polluted or clean relevant conditions while toluene is an organic precursor used for photochemistry. NH3 and NOx concentration is monitored online continuously for 3 hours while collected samples during the reaction time are weighted and then analyzed by Ion chromatography (IC) to determine cations (NH4+) and anions (SO42-, NO3-). As a result, a significant partitioning of semi-volatile inorganic from the gas phase to particles under high humidity with a present of seed particle. While organic did not show evidence of its effects on ammonium and nitrate formation, a lower concentration of gaseous caused a time delay on the formation of the inorganic compound in the particle phase.

Real-time and Label Free Detection of Biochemical Reaction Using Liquid Crystals

DUONG SONG THAI DUONG Gachon University 2021 국내석사

In this thesis, we describe the research of nematic liquid crystals (LCs)-based sensing technology to monitor biochemical reactions by investigating the optical response of LCs. Biochemical reactions could be sensitively detected with a polarizing light microscope due to the birefringence as well as the long-range anchoring transition of LCs. Herein, we observed the orientational transition of LCs influenced by (i) the enzymatic reactions of Human arginase 1 and ʟ-Arginine, and (ii) the biomolecular interactions of MUC1 and its specific aptamer. In (i) system, stearic acid is doped with 5CB to form a pH-dependent LCs. ʟ-arginine were hydrolyzed in the catalysis of ARG1 leading to the increase in pH. Then, stearic acid is deprotonated and adsorbed at the interface and change the LCs orientation. The shift on the LC alignment could be identified by an optical signal by using a polarizing light microscope. In (ii) system, the alignment molecules and bioreceptor are co-immobilized on the glass surface. While the alignment molecules anchor nematic LCs, bioreceptor capture the target. The surface topography changes and leads to the disrupt of LCs anchoring. The change in the LCs orientational could be observed by using a crossed polarizers. We described the LC-based sensing systems as a real-time and sensitive technique for imaging biochemical reactions.

Development of Liquid Crystal-Based Sensing Platforms for Real-Time Detection of Biomolecules and Environmental Contaminants

DUONG SONG THAI DUONG 가천대학교 글로벌캠퍼스 일반대학원 2025 국내박사

This dissertation presents research work dealing with rapid diagnostic methods for the monitoring of biomolecules and environmental contaminants through the use of liquid crystal (LC)-based sensing technology. The inherent elasticity of LC enables the amplification of slight changes in surface anchoring at the interface between the LC sensor and the target biomolecule, which facilitates optical detection using polarized optical microscopy (POM). Chapter 1 introduces the concept of biosensors, with a particular focus on LC-based biosensors and the properties of LC materials. A biosensor is defined as an analytical instrument consisting of components including: receptor, transducer, and signal processor, which together provide increased sensitivity and reliability. LC-based sensors detect analytes through observable optical changes, making them an effective tool for examination environmental, early diagnosis due to their simplicity and cost-effectiveness. Liquid crystalline materials can exist in different phases, transitioning between ordered and disordered states, and are categorized into thermotropic and lyotropic types. Birefringence, a key property of LCs, enables the study of optical variations in response to molecular ordering. Chapter 2 presents an LC-based assay for quantification sialic acid (SA). The sensor machenism is based on a synthetic bimetallic Co/2Fe MOF mimicking an oxidase and the NANA aldolase. After functionalization on TEM gold grids, the free SA was converted to pyruvate and N-acetyl-D- mannosamine in an aqueous solution by the catalysis of NANA aldolase. The subsequent Co/2Fe MOF-mediated reaction converts pyruvate to acetyl phosphate and H2O2, resulting in a sharp change in pH. This pH change is optically detected using LC doped with stearic acid. Chapter 3 presents a sensor that utilizes the electrostatic interactions between Cu²⁺ ions and DOPG molecules to detect glyphosate. The effectiveness of the sensor was qualitatively and quantitatively validated, with applications for environmental monitoring and agriculture investigated. Chapter 4 presents a qualitative detection method for phosphatidylserine (PS) using a waveform surface modification in conjunction with an Annexin V amplification system. The presence of annexin V contributed to enhance the optical signal, which significantly influenced mobilization of LCs and correlated with the changes in surface roughness. The sensor effectively detects PS by recognizing shifts in LC alignment. In chapter 5, an aptasensor was introduced with the aim to monitor acetamipride (ACE) using aptamers. ACE-antibody binding induces surface topography changes, resulting on potential repositioning of the LCs and changes in the optical signals. The observed optical intensities correlate with the ACE concentrations and allow a quantitative analysis of ACE in different samples.

TOWARD A LOW ENERGY CONSUMPTION SINGLE CELL TESTING SYSTEM

DUONG DUY DUONG 가천대학교 일반대학원 2022 국내박사

The concept of microfluidic devices and its applications are well established and poses leading contributions in industries owing to great prospective in varied applications like biomedical research, clinical diagnostics, immunoassays, and many more. The inclination for miniaturized and simplify instruments for faster, cost-effective fabrication, and deployment is the primary causal objective for this thesis. Before this objective can be apprehended considerable research and development must be performed in several areas where existing microfluidic design suffer limitations, for example expensive materials and overly design lead to low level of reproducibility. When arrested this will allow the use of less expensive and more accessible method to fabricating and test a microfluidic system. The aim of the thesis is to present a few projects where design, fabrication and representation of such microfluidic devices have been performed. The final goal of my work is conceptualization of a single cell analysis system which can be easy to fabricate and operate on a minimal amount of energy. In the first chapter, I summarized the work that has been elaborately discussed in the following chapters. Chapter 2 will talk about a microfluidic device with the function of single cell encapsulation and passively sorting encapsulated cell utilizing inertia. The output encapsulated cells can be used in other applications such as molecular diagnostic to tissue engineering. In the next chapter, I introduce a system of high throughput electrical cell lysis microfluidic device. The device can be used to quickly lyse a large volume cell for the purpose of harvesting the cell’s content. In the final chapter, a microfluidic device that use the energy harvested from small temperature differences to extract cell contents was developed. This is designed with the aim of a quick and easy deployment of the system with no requirement of previous training.

Crosstalk between glutathione peroxidase-1 gene and protein kinase Cδ gene in the methamphetamine-induced dopaminergic neurotoxicity

DUONG XUAN CHU 강원대학교 2008 국내박사

Escalating evidences suggest that oxidative stress is involved in methamphetamine (MA)-induced neurotoxicity, and that peroxides including H2O2 play a crucial role in this toxicity. Enzymatic antioxidants, such as catalase, and glutathione peroxidase (GPx), provide a first line of defense against H2O2. A selenium-dependent GPx (GPx-1) out of GPx isozymes is considered as a major H2O2 scavenger in the brain. In the present study, it was asked whether GPx-1 gene affects MA-induced dopaminergic neurotoxicity, and whether protein kinase C (PKC) affects this toxicity, since it was suggested that PKC might contribute to dopaminergic toxicity. Treatment with MA (8.0 mg/kg, i.p. x 4) resulted in the decrease in striatal GPx-1-like immunoreactivity in the GPx-1 (+/+) mice. MA treatment produced hyperthermia, dopaminergic toxicity [as measured by dopamine turnover rate, tyrosine hydroxylase (TH) activity, TH-like immunoreactivity and TH phosphorylation at serine residue 31, 40], oxidative stress [as measured by protein carbonyl and lipid peroxidation], neuroinflammation [as measured by cyclooxygenase-2 (COX-2)-, interleukin-6 (IL-6)-, tumor necrosis factor-α (TNF-α)-, and interferon-γ (IFN-γ)-like immunoreactivity], microgliosis [as labeled by F4/80- or ionized calcium-binding adaptor molecule-1 (Iba-1)-like immunoreactivity], and reduction of neurotrophic factors [as measured by brain-derived neurotrophic factor (BDNF)- and glial cell line-derived neurotrophic factor (GDNF)-like immunoreactivity]. Intrastriatal microinjection with chelerythrine (a pan-inhibitor of PKC) or rottlerin (an inhibitor of PKCδ), but not with Go6976 (a co-inhibitor of PKCα and PKCβ), hispidine (an inhibitor of PKCβ) or PKCζ pseudosubstrate (an inhibitor of PKCζ), attenuated MA-induced hyperthermia and behavioral impairments (as measured by locomotor activity and rota-rod performance). Consistently, treatment with MA significantly increased striatal expressions of PKCδ and cleaved PKCδ, whereas there was no significant change in the expressions of other PKC isozymes. These findings were more pronounced in GPx1 (-/-) mice than GPx1 (+/+) mice. Intrastriatal microinjection with chelerythrine or rottlerin significantly attenuated MA-induced increases in the PKCδ and cleaved PKCδ. As shown in case of chelerythrine or rottlerin, GPx-mimics, such as ebselen and acetylsalicylic acid maltol ester (AME), significantly attenuated MA-induced hyperthermia, behavioural impairments, dopaminergic toxicity, oxidative stress, neuroinflammation, microgliosis, increased striatal expressions of PKCδ and cleaved PKCδ, and decreased expressions of neurotrophic factors. In addition, treatment with ebselen, AME or rottlerin significantly prevented MA-induced decreases in mouse double minute (MDM)-2 phosphorylation as well as increases in p53 expression in the striatum of the mice. The protective effects of ebselen, AME or rottlerin were less pronounced in GPx-1 (-/-) mice than GPx-1 (+/+) mice. GPx-1-like immunoreactivity was restored by intrastriatal GPx-1 gene transfection with GPx1 gene-encoded adenovirus vector in GPx1 (-/-) mice. Intrastriatal GPx-1-transfected GPx1 (-/-) mice were less susceptible to MA-induced hyperthermia, behavioural impairment, decrease in TH-like immunoreactivity, and increase in PKCδ expression as compared with those of control vector-transfected GPx1 (-/-) mice. In addition, MA-induced hyperthermia, behavioural impairment, increase in dopamine turnover rate, decrease in GPx-1 expression were less pronounced in PKCδ (-/-) mice than PKCδ (+/+) mice. The results suggest that GPx1 gene is an essential factor for blocking MA-induced dopaminergic toxicity, and that PKCδ gene is involved in this pathogenesis. GPx-mimic compounds attenuate MA neurotoxicity via inhibiting PKCδ expression, oxidative stress, and neuroinflammation. 지금까지의 많은 연구결과들이 oxidative stress가 메탐페타민 (MA)에 의한 신경독성에 관여함을 제시하고 있으며, 특히 H2O2를 비롯한 과산화물이 결정적인 작용을 수행함. Catalase 혹은 glutathione peroxidase (GPx)와 같은 항산화 효소들이 H2O2를 일차적으로 소거하는데, 그 중에서도 셀레늄 의존형 GPx (GPx-1)가 중추신경계의 가장 주요한 H2O2 소거 효소 중 하나임. 이번 연구에서는 GPx-1 유전자가 MA에 의한 도파민성 신경독성에 어떠한 역할을 수행하는지 규명하였으며, protein kinase C (PKC)가 도파민성 신경독성 과정에 관여한다는 보고가 있었기 때문에, MA에 의한 독성에서 PKC 유전자의 역할에 대한 연구도 이루어졌음. MA (8.0 mg/kg, i.p. x 4)를 투여한 GPx-1 (+/+) 생쥐의 선조체에서 GPx-1 면역활성의 감소가 관찰되었음. MA 투여는 고체온증, 도파민성 독성 [도파민 회전율의 증가, tyrosine hydroxylase (TH)의 활성 및 발현, TH serine 31번 및 40번 잔기의 인산화의 감소], oxidative stress (단백질 산화와 지질과산화의 증가), 신경염증성 반응 (cyclooxygenase-2, interleukin-6, interferon-γ, tumor necrosis factor-α의 변화), 소교세포 활성화 (F4/80- 혹은 ionized calcium-binding adaptor molecule-1-면역활성의 증가) 및 신경성장 인자의 감소 (brain-derived neurotrophic factor- 및 glial cell line-derived neurotrophic factor-면역활성의 감소)를 유도하였음. Chelerythrine (PKC 억제제) 혹은 rottlerin (PKCδ 억제제)의 선조체 내 투여는 MA에 의한 고체온증과 행동이상 (자발운동량의 감소 및 rota-rod test에서의 수행능력 저하)을 유의하게 감소시킨 반면, Go6976 (PKCα와 PKCβ의 동시 억제제), hispidine (PKCβ 억제제), 혹은 PKCζ pseudosubstrate (PKCζ 억제제)의 투여는 유의한 효과를 나타내지 않았음. 이와 마찬가지로, MA 투여 후에 선조체에서 PKCδ 및 cleaved PKCδ의 발현은 유의하게 증가하였으나, 다른 PKC isozyme들의 발현은 유의하게 변화하지 않았음. 그런데, 이러한 경향이 GPx-1 (+/+) 생쥐에 비하여 GPx-1 (-/-) 생쥐에서 더욱 현저하였음. 또한, Chelerythrine 혹은 rottlerin의 투여는 MA에 의해서 증가된 PKCδ 및 cleaved PKCδ의 발현을 유의하게 억제하였음. 본 연구에서 ebselen 혹은 acetylcalicylic acid maltol ester (AME)와 같은 GPx 유사 작용제의 투여가 MA로 인해 유도된 고체온증, 행동이상, 도파민성 독성, oxidative stress, 신경염증, 소교세포 활성화, PKCδ 및 cleaved PKCδ의 발현 증가 및 신경성장인자의 발현 감소를 유의하게 억제하였음. 더불어 ebselen, AME 혹은 rottlerin의 투여는 MA에 의해서 선조체에서 나타나는 mouse double minute (MDM)-2 인산화의 감소와 p53 발현의 증가를 유의하게 억제하였음. 이러한 ebselen, AME 혹은 rottlerin의 보호효과는 GPx-1 (+/+) 생쥐에서 GPx-1 (-/-) 생쥐에 비하여 더욱 현저하였음. GPx-1 (-/-) 생쥐에 GPx-1 gene-encoded adenovirus vector를 이용하여 GPx-1 유전자를 선조체 내에 주입한 결과, GPx-1 유전자의 발현이 유의하게 증가하였는데, control vector가 주입된 GPx-1 (-/-) 생쥐에 비하여 GPx-1 유전자가 주입된 GPx-1 (-/-) 생쥐에서 MA에 의한 고체온증, 행동이상, 도파민성 신경독성 및 PKCδ의 발현 증가가 유의하게 억제되었음. 또한, MA에 의한 고체온증, 행동이상, 도파민 회전율의 증가와 선조체에서의 GPx-1 발현의 감소가 PKCδ (-/-) 생쥐에서 PKCδ (+/+) 생쥐에 비하여 유의하게 억제되었음. 이상의 결과는 GPx-1 유전자가 MA에 의한 도파민성 신경독성을 억제하는 중요한 인자임과 동시에 PKCδ 유전자가 MA에 의한 신경독성의 발현과정에 관여함을 제시함. GPx-1 유사 작용제는 PKCδ 발현, oxidative stress 및 신경염증을 억제함으로써 MA에 의한 신경독성을 억제하였음

(A) study on the high performance electromagnetic energy harvesting technology for vehicle suspension

Duong Minh Trung University of Science and Technology 2019 국내박사

Owing to the shortage of fossil fuel and a significant increase in the number of electric vehicles, it is mandatory to find alternative energy supplies to extend the mileage or operating time. Recovery of the wasted energy has been tremendously investigated for different sources, such as regenerative braking energy, thermal energy, and vibration energy from the suspension system. According to recent reports, harvestable power from the suspension system in a typical passenger car is between 100 and 400 W. This leads to a fuel efficiency improvement in the hybrid and electric vehicles by 7-10%. The major drawback of this technology lies in the difficulty of enhancing output power for a given space. The objective of this dissertation is the development of a high performance electromagnetic shock absorber applied to the vehicle energy harvesting technology. Operating conditions are based on the assumption that when a passenger car is moving on a road class C at a speed of 96 km/h, vibration speed, vibration frequency, and peak-to-peak stroke length on the shock absorber are 0.25 m/s, 10 Hz and 11.25 mm, respectively. Design targets for maximum and average output power are 250 and 100 W, maximum and average power density are 0.250 and 0.100 W/cm3, respectively. In this dissertation, direct-drive using a linear tubular generator is selected due to its simple structure, elimination of the transmission mechanisms, fast responses, etc. Different from most of the conventional devices, the novel machine combines both mechanical damper and electrical generator. Because of this specific configuration, the electromagnetic force has to be minimized to ensure safety and driving comfort. Based on the actual size of a commercial shock absorber in an SUV-Korando car, available space, and dimensions of the proposed machine are decided. To achieve the design targets, various topologiess including coreless model, cored model, inner and outer permanent magnet model, slot-pole combination, and number of phases are investigated. On top of that, to significantly increase the power density, a hybrid-permanent magnet structure is innovated. To simultaneously maximize output power and minimize electromagnetic force, multi-objective optimization based response surface method is implemented. Magnetic design and analytical prediction of performance are performed using an extensive finite-element analysis. Prototypes of the coreless and cored model are fabricated to evaluate the performance and verify the validity of the analysis. Experimental results are well-matched with analysis and all the design targets are successfully achieved.

Resident’s Attitudes Toward Dark Tourism Development, The Case of Vietnam : Comparing Long-term and Short-term Residents

Duong Thi Kim Oanh 세종대학교 대학원 2019 국내석사

This research is based on the interest found in the class of university. Along with the passion involved in the study of hospitality management and in addition, the article is based on the desire to explore new directions for the development of dark tourism based on remnants of war in Vietnam. The main research content of the study is to focus on the attitudes and opinions of local residents where the remnants of war exist for the development of war-based tourism. Also consider attitudes towards the ongoing tourism development in the locality. The way people perceive this humanitarian problem is given to conduct survey research, data collection and evaluation.

Novel Estimation Technique for the State-of-Charge of the Lead-Acid Battery by Using EKF Considering Hysteresis Phenomenon : 히스테리시스 현상을 고려한 확장칼만필터를 이용한 새로운 납축전지의 충전상태 추정방법

DUONG VAN HUAN 숭실대학교 대학원 2013 국내석사

State-State-of-Charge (SOC)는 전기자동차의 운전범위를 평가하고 기존의 엔진 자동차의 발전기를 제어하는 하나의 중요한 지표이다. 그러므로 SOC의 정확한 평가는 에너지의 효과적인 활용뿐 아니라 파워트레인에 생길 수 있는 심각한 문제의 발생 방지와 배터리의 수명연장을 위해서 중요하다. 히스테리시스의 현상은 특히 자주 충방전하는 배터리에 있어서 SOC추정에 상대적으로 커다란 오차를 발생시키다. 본 논문은 잘 알려진 확장 칼만 필터와 납축전지의 전기적 등가회로 모델을 이용하여 히스테리시스 특성을 고려한 새로운 SOC추정 방법에 대해서 제안하였다. 히스테리시스 효과는 부분 사이클 동안 누적된 전하량의 표준화된 적산에 의해 모델링된다. 제안된 알고리즘의 유효성은 실험을 통해 검증하였다. State-of-charge (SOC) is one of the significant indicators to estimate the driving range of the electric vehicle and to control the alternator of the conventional engine vehicles as well. Therefore its precise estimation is crucial not only for utilizing the energy effectively but also preventing critical situations happening to the powertrain and lengthening the lifetime of the battery. The hysteresis phenomenon causes relatively large errors in estimating the SOC of the battery especially under the frequent discharge/charge. This paper proposes a novel estimation technique for the SOC of the Lead-Acid battery by using a well-known Extended Kalman Filter (EKF) and an electrical equivalent circuit model of the Lead-Acid battery considering hysteresis characteristics. The hysteresis effect is modeled by the calculation of the normalized integration of the charge throughput during the partial cycle. The validity of the proposed algorithm is verified through the experiments.