http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
不正咬合者의 思春期成長과 手腕 部骨 成熟段階에 關한 累年的 硏究
李東柱,金炯一 대한치과교정학회 1989 대한치과교정학회지 Vol.19 No.3
To predict the pubertal growth peak in stature and study the skeletal maturity degree using hand-wrist radiograph, the author used the 70 malocclusions (male 24, female 46). After longitudinal measurement of stature and skeletal maturity indicators of hand-wrist radiographs were taken during 4 years, the results were as follows. 1) The pubertal growth peak in stature occurred mainly at SMI 6-7 (56.5%) in female, SMI 5-6 (37.5%), 6-7(37.5%) in male (Table 5). 2) It was suggested that the pubertal growth peak in stature was already passed, if SMI 8 occurred. 3) Ages of SMI in female were about 2 years earlier than those of SMI in male, and the sexual difference was gradually decreased in puberty. 4) Duration of SMI was longest at SMI 6-7 in both sex and the mean was 8.5 months. After this stage, the velocity of skeletal maturity in female was decreased than in male. 5) The correlation coefficient between each SMI and pubertal growth peak was very high(Table 8).
Stabilization of Retinol through Incorporation into Liposomes
(Seung Cheol Lee),(Hyun Gyun Yuk),(Dong Hoon Lee),(Kyung Eun Lee),(Yong Ll Hwang),(Richard D. Ludescher) 생화학분자생물학회 2002 BMB Reports Vol.35 No.4
Chemical and photochemical processes during storage and preparation rapidly degrade retinol, the most active form of vitamin A. Therefore, the efficacy of incorporation into liposomes in order to modulate the kinetics of retinol degradation was investigated. Retinol was readily incorporated into multilamellar liposomes that were prepared from soybean phosphatidylcholine; the extent of the incorporation was 98.14±0.93% at pH 9.0 at a ratio of 0.01 : 1 (wt : wt) retinol : phospholipid. It was only marginally lower at higher retinol concentrations. The pH of the hydration buffer had a small effect. The incorporation efficiency ranged from 99.25±0.47% at pH 3 to 97.45±1.13 % at pH 11. The time course of the retinol degradation in the aqueous solution in liposomes was compared to that of free retinol and free retinol with atocopherol under a variety of conditions of pH (3, 7, and 11), temperature (4, 25, 37, and 50℃), and light exposure (dark, visible, and UV). The retinol that was incorporated into the liposomes degraded significantly slower than the free retinol or retinol with α-tocopherol at pH 7 and 11. At pH 3, where the free retinol degrades rapidly, the degradation kinetics were similar in liposomes and the presence of α-tocopherol. At pH 7.0 and 4℃ in the light, for example, free aqueous retinol was completely degraded within 2 days, while only 20% of the retinol in the liposomes were degraded after 8 days. In general, the protective effect of the liposome incorporation was greater at low temperatures, at neutral and high pH, and in the dark. The results suggest that protection is greater in the solid, gel phase than in the fluid liquid crystalline phase lipids. These results indicate that the incorporation into liposomes can extend the shelf-life of retinol under a variety of conditions of temperature, pH, and ambient light conditions.
형질 전환된 Saccharomyces cerevisiae의 leucocin A항균 활성도에 대한 탄소원의 영향
이성일,박진용,정종근,이동근,이상현,하종명,하배진,이재화,Lee Sung-ll,Park Jin-Yong,Jung Jong-Ceun,Lee Dong-Ceun,Lee Sang-Hyeon,Ha long-Myung,Ha Bae-Jin,Lee Jae-Hwa 한국생명과학회 2005 생명과학회지 Vol.15 No.6
B. subtilis에 대한 항균 활성을 파악하기 위하여 bacteriocin의 일종인 leucocin A로 형질전환된 효모를 배양 시간별로 채취하여 광학밀도, 총 분비 단백질량, 단백질 분해효소 그리고 향균활성을 측정했다. Sucrose > glycerol > glucose > fructose 순으로 세포 증식이 높게 확인되었고, glyceroldmf 제외한 탄소원에서 총분비 단백질은 배양 15 시간까지 증가되었다. Leucocin A의 향균활성(antibacterial activity)은 성장양상, 총 분비 단백질과 비례 하였으며 배양 15 시간에 glucose와 sucrose에서 배양한 상등액이 B. subtilis 성장을 가각 $65.5\%$, $72.6\%$ 감소하는 것으로 나타났다. 정지기 이후에는 향균활성이 급속히 감소하였는데, 이는 항균활성을 보이는 leucocin A가 단백질이고, 단백질 분해효소가 정지기 이후 증가한 것에 의한 것으로 사료된다. 본 연구는 향후 식품산업뿐만 아니라 항생제 생산 사용되기 위한 산업적 기초 자료로써 이용 될 것이다. The aim of this study was to increase production of leucocin A, a kind of bacteriocin, in a transformed variety of S. cerevisiae. We investigated optical density, total secreted protein, protease activity, and antibacterial activity for the transformed S. cerevisiae in different carbon sources. The production of leucocin A growth-associated, and antibacterial activity, according to carbon source, was in the order of sucrose, glucose, glycerol, and fructose. Antibacterial activity was $10.6\%$ higher in the presence of sucrose than glucose. This is the first report regarding the effect of carbon sources on the production of leucocin A in transformed S. cerevisiae, as far as we ascertain. Our results could prove useful in the industrial production of natural preservatives.
桔梗이 人體 肺癌細胞에 미치는 영향에 대한 실험적 연구
이성열,김원일,박동일 대한동의생리학회,대한동의병리학회 2003 동의생리병리학회지 Vol.17 No.4
Platycodi Radix, the root of Platycodon grandiflorum, commonly known as Doraji, is used as a traditional oriental medicine. Extracts from the roots of P. grandiflorum have been reported to have wide ranging health benefits. In the present study, we investigated the effects of an aqueous extract from the roots of P. grandiflorum (AEPG) on the growth of human lung carcinoma A549 cells. results obtained are as fellow; AEPG treatment resulted in the inhibition of the cell viability of A549 cells in a concentration-dependent manner. Upon treatment with AEPG, A549 cells developed many of the hallmark features of apoptosis, including condensation of chromatin. Flow cytometry analysis confirmed that AEPG increased populations of apoptotic-sub G1 phase. Western blot and RT-PCR analyses indicated that the expressions of Bcl-2 was down-regulated but Bax was up-regulated in AEPG-treated A549 cells. AEPG-induced apoptotis of A549 cells was associated with proteolytic cleavage and activation of caspase-3, release of cytochrome C from mitochondria into cytosol and down-regulation of Akt and phospho-Akt proteins in a dose-dependent manner. Induction of apoptosis by AEPG treatment was associated with inhibition and/or degradation of apoptotic target proteins such as poly(ADP-ribose) polymerase, β -catenin and phospholipase C-γ1. AEPG treatment inhibited the levels of cyclooxygenases protein of A549 cells, which was associated with the inhibition of prostaglandin E2 accumulation in a concentration-dependent fashion. Taken together, these findings suggest that P. grandiflorum has strong potential for development as an agent for prevention against human lung cancer.