Heme oxygenase-1 induced by desoxo-narchinol-A attenuated the severity of acute pancreatitis via blockade of neutrophil infiltration

Bae, Gi-Sang,Kim, Dong-Goo,Jo, Il-Joo,Choi, Sun-Bok,Kim, Myoung-Jin,Shin, Joon Yeon,Kim, Dong-Uk,Song, Ho-Joon,Joo, Myungsoo,Park, Sung-Joo ELSEVIER 2019 INTERNATIONAL IMMUNOPHARMACOLOGY Vol.69 No.-

<P><B>Abstract</B></P> <P>Heme oxygenase-1 (HO-1) has an anti-inflammatory action in acute pancreatitis (AP). However, its mechanism of action and natural compounds/drugs to induce HO-1 in pancreas are not well understood. In this study, we investigated the regulatory mechanisms of HO-1 during AP using desoxo-narchinol-A (DN), the natural compound inducing HO-1 in the pancreas. Female C57/BL6 Mice were intraperitoneally injected with supramaximal concentrations of cerulein (50 μg/kg) hourly for 6 h to induce AP. DMSO or DN was administered intraperitoneally, then mice were sacrificed 6 h after the final cerulein injection. Administration of DN increased pancreatic HO-1 expression through activation of activating protein-1, mediated by mitogen-activated protein kinases. Furthermore, DN treatment reduced the pancreatic weight-to-body weight ratio as well as production of digestive enzymes and pro-inflammatory cytokines. Inhibition of HO-1 by tin protoporphyrin IX abolished the protective effects of DN on pancreatic damage. Additionally, DN treatment inhibited neutrophil infiltration into the pancreas via regulation of chemokine (C-X-C motif) ligand 2 (CXCL2) by HO-1. Our results suggest that DN is an effective inducer of HO-1 in the pancreas, and that HO-1 regulates neutrophil infiltration in AP via CXCL2 inhibition.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Desoxo-narchinol-A (DN) is a natural compound of HO-1 inducer in pancreas. </LI> <LI> Mechanism of DN-induced HO-1 is mediated by MAPK/Activator Protein-1/HO-1 signaling. </LI> <LI> DN-induced HO-1 blocks neutrophil infiltration into pancreas via inhibition of CXCL2. </LI> <LI> DN inhibits cerulein-induced acute pancreatitis (AP) and AP-associated lung injury. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

12M의 고해상도 360° 카메라를 사용한 주차장의 14면 주차 상태 판단 프로그램

이영지(Young-Ji Lee),이희열(Hee-Yeol Lee),고태영(Tae-Young Ko),곽동훈(Dong-Hoon Kwak),김재형(Jae-Hyung Kim),김주호(Joo-Ho Kim),오승진,이태윤(Tae-Yoon Lee),박상민(Sang-Min Park),이승호(Seung-Ho Lee) 대한전자공학회 2019 대한전자공학회 학술대회 Vol.2019 No.6

In this paper, we propose a program to identify 14 parking status in a parking lot using a high resolution 360° camera of 12M. The proposed program consists of three steps: Match with plane image, parking area detection algorithm, and discrimination of parking using learning method. Tests on a model car to evaluate the program to identify 14 parking status in a parking lot using a high-resolution 360° camera of 12M showed 100% accuracy for both parking and double parking. Therefore, the effectiveness of a program to identify 14 parking status in a parking lot using a high-resolution 360° camera of 12M proposed in this paper has been proved.

국립공원 내 공중화장실에 대한 탐방객 인식에 관한 연구 : 지리산국립공원을 중심으로

이호(Ho Lee),이승록(Seung-Rok Lee),박기현(Ki-Hyun Park),박은희(Eun-Hee Park),차진열(Jin-Yeol Cha),심규원(Kyu-Won Sim),강동원(Dong-Won Gang) 국립공원연구원 2011 국립공원연구지 Vol.2 No.1

본 연구는 공원 내 공중화장실에 대한 탐방객의 인식이 만족도에 미치는 영향을 규명하고자 수행하였다. 현지 설문조사는 지리산국립공원의 성삼재와 중산리 일원을 방문한 탐방객 292명을 대상으로 이루어졌다. 분석결과, 공중화장실의 관리상태, 외관, 디자인 요인 모두 탐방객의 공중화장실 이용 만족에 영향을 주는 것으로 분석되었으며, 그 중 관리상태가 가장 큰 영향을 주는 것으로 나타났다. 따라서 탐방객의 인식과 만족을 향상시키기 위해서는 공중화장실의 청결한 관리 및 유지, 악취 방지, 소모품의 원활한 공급 등에 우선순위를 두어 집중적으로 관리할 필요가 있는 것으로 나타났다. This study conducts to investigate the relationship between visitors’ perception and satisfaction. Data of 292 visitors were collected from Seongsamjae and Jungsanri in Jirisan National Park. The results showed that maintenance, outlook and design of public toilet are positively related to visitors’ satisfaction. One of the major research findings is that the maintenance of public toilet most affects visitors’ satisfaction. Therefore, the park managers need to put great emphasis on the cleanliness and maintenance, preventing stinks, consumable supplies of public toilet in order to enhance visitors’ perception and satisfaction.

Carbon monoxide prevents TNF-α-induced eNOS downregulation by inhibiting NF-κB-responsive miR-155-5p biogenesis

Choi, Seunghwan,Kim, Joohwan,Kim, Ji-Hee,Lee, Dong-Keon,Park, Wonjin,Park, Minsik,Kim, Suji,Hwang, Jong Yun,Won, Moo-Ho,Choi, Yoon Kyung,Ryoo, Sungwoo,Ha, Kwon-Soo,Kwon, Young-Guen,Kim, Young-Myeong Nature Publishing Group 2017 Experimental and molecular medicine Vol.49 No.11

<P>Heme oxygenase-1-derived carbon monoxide prevents inflammatory vascular disorders. To date, there is no clear evidence that HO-1/CO prevents endothelial dysfunction associated with the downregulation of endothelial NO synthesis in human endothelial cells stimulated with TNF-α. Here, we found that the CO-releasing compound CORM-2 prevented TNF-α-mediated decreases in eNOS expression and NO/cGMP production, without affecting eNOS promoter activity, by maintaining the functional activity of the <I>eNOS</I> mRNA 3′-untranslated region. By contrast, CORM-2 inhibited MIR155HG expression and miR-155-5p biogenesis in TNF-α-stimulated endothelial cells, resulting in recovery of the 3′-UTR activity of <I>eNOS</I> mRNA, a target of miR-155-5p. The beneficial effect of CORM-2 was blocked by an NF-κB inhibitor, a miR-155-5p mimic, a HO-1 inhibitor and siRNA against HO-1, indicating that CO rescues TNF-α-induced eNOS downregulation through NF-κB-responsive miR-155-5p expression via HO-1 induction; similar protective effects of ectopic HO-1 expression and bilirubin were observed in endothelial cells treated with TNF-α. Moreover, heme degradation products, except iron and <I>N</I>-acetylcysteine prevented H<SUB>2</SUB>O<SUB>2</SUB>-mediated miR-155-5p biogenesis and eNOS downregulation. These data demonstrate that CO prevents TNF-α-mediated eNOS downregulation by inhibiting redox-sensitive miR-155-5p biogenesis through a positive forward circuit between CO and HO-1 induction. This circuit may play an important preventive role in inflammatory endothelial dysfunction associated with human vascular diseases.</P>

버스정보시스템(BIS)을 위한 모바일 환경의 시각화 기법 연구

박동규,박진화,이수진,박은호,류동성,어윤 국립7개대학공동논문집간행위원회 2004 공업기술연구 Vol.4 No.-

In this paper we describe a visualization method for Bus Information System(BIS) on Mobile Environment. A BIS is a key service for Intelligent Transport System(ITS) on public transportation area. We present a client-server based visualization technique for bus route information over Internet Protocol and user-oriented regional information service with a voice and animation. We also describe the design and implementation of this kind of schematic map generation system with icon and label.

NGS_SNPAnalyzer: a desktop software supporting genome projects by identifying and visualizing sequence variations from next-generation sequencing data

Dong‑Jun Lee,Taesoo Kwon,Chang‑Kug Kim,Young‑Joo Seol,Dong‑Suk Park,Tae‑Ho Lee,Byung‑Ohg Ahn 한국유전학회 2020 Genes & Genomics Vol.42 No.11

Background Sequence variations such as single nucleotide polymorphisms are markers for genetic diseases and breeding. Therefore, identifying sequence variations is one of the main objectives of several genome projects. Although most genomeproject consortiums provide standard operation procedures for sequence variation detection methods, there may be differencesin the results because of human selection or error. Objective To standardize the procedure for sequence variation detection and help researchers who are not formally trainedin bioinformatics, we developed the NGS_SNPAnalyzer, a desktop software and fully automated graphical pipeline. Methods The NGS_SNPAnalyzer is implemented using JavaFX (version 1.8); therefore, it is not limited to any operatingsystem (OS). The tools employed in the NGS_SNPAnalyzer were compiled on Microsoft Windows (version 7, 10) andUbuntu Linux (version 16.04, 17.0.4). Results The NGS_SNPAnalyzer not only includes the functionalities for variant calling and annotation but also providesquality control, mapping, and filtering details to support all procedures from next-generation sequencing (NGS) data to variantvisualization. It can be executed using pre-set pipelines and options and customized via user-specified options. Additionally,the NGS_SNPAnalyzer provides a user-friendly graphical interface and can be installed on any OS that supports JAVA. Conclusions Although there are several pipelines and visualization tools available for NGS data analysis, we developedthe NGS_SNPAnalyzer to provide the user with an easy-to-use interface. The benchmark test results indicate that theNGS_SNPAnayzer achieves better performance than other open source tools.

요통 환자의 기립위 및 좌,우 외측굴곡시 방사선 사진을 통한 요추 회전변위 분석

허동석 ( Dong Seok Heo ),박석우 ( Seok Woo-park ),박영회 ( Young Hoi Park ),금동호 ( Dong Ho Keum ) 한방재활의학과학회 2003 한방재활의학과학회지 Vol.13 No.2

Objectives : Rotation on vertebra body is common reason causing low back pain and leg radiating pain patients. The present study investigated analysis of the radiological findings on lumbar vertebra rotation between erect position and erect both lateral bending position lumbar AP views in low back pain patients. Method : The study population consisted of 70 patients(outpatients and inpatients clinic of the Dept. of Oriental Rehabilitation Medicine, Dong-Guk Oriental Medical Hospital in Bundang) who were evaluated with X-ray findings of erect position and erect both lateral bending position lumbar AP views. First, patients were measured erect position lumbar AP views by Nash-Moe`s degree. Second, patients were measured erect both lateral bending position lumbar AP views by intervertebral disc space at convexity region and rotation on lumbar vertebral body. They divided into four groups by type of Grice and Cassidy`s classification. Results : 1. The distribution of sex in the low back pain with leg radiating pain was male 30(42.86%) cases, female 40(57.14%) cases. According to age, 30-40s 23(32.9%) cases, 40-50s 15(21.4%) cases, 20-30s 13(18.6%) cases, 50-60s 9(12.9%) cases, 60-70s 5(7.1%) cases, 70-80s 3(4.3%) cases, each 10-20s and 80-90s one case (1.4%). 2. According to Nash-Moe`s degree by erect position lumbar AP view, the grade 0 was male 8(11.4%) cases, female 10(14.3%) cases, the grade Ⅰ was male 17(24.3%) cases, female 22(31.4%) cases, the grade Ⅱ was male 5(7.1%) cases, female 8(11.4%) cases. 3. According to symptoms, first, patients with low back pain were male 22(31.4%) cases, female 20(28.6%) cases. second, patients with leg radiating pain were male 8(11.4%) cases, female 20(28.6%) cases. 4. According to type of Grice and Cassidy`s classification by erect both lateral bending position lumbar AP views, the type Ⅰ was normal group 7(10.0%) cases, the type Ⅲ and Ⅳ were abnormal group each 15(21.4%) cases, 48(68.6%) cases. 5. According to abnormal group in Nash-Moe`s degree by erect position lumbar AP view, the grade Ⅰ was 39(55.7%) cases and then abnormal type Ⅲ, Ⅳ of Grice and Cassidy`s classification were 5(7.1%) cases, 33(47.1%) cases, the grade Ⅱ was 13(18.6%) cases and then abnormal type Ⅲ, Ⅳ of Grice and Cassidy`s classification were 1(1.4%) case, 12(7.1%) cases. So the relationship between rotation of vertebra by erect position lumbar AP view and type of Grice and Cassidy`s classification by erect both lateral bending position lumbar AP views had significant correction(p<0.05). 3 6. According to analysis of symptoms and X-ray findings by erect both lateral bending position at convexity region, patients with leg radiating pain were 28 cases. In the total of 28 cases, 25 cases had shown rotation of vertebra and intervertebra disc space narrowed at convexity region, while 3 cases didn`t have shown. 7. According to abnormal rotation of vertebral body on erect position, the number of group L4 was 48(32.7%) cases the most, group L3 was 43(29.3%) cases, the next group L2 was 30(20.4%) cases. Group L4, L3, L2 had significantly connection with fixation group on erect both lateral bending position(p<0.05). Conclusions : We concluded that the abnormal rotation of lumbar vertebral body had connection with the low back pain and leg radiating pain patients. So we must regard functional X-ray such as erect both lateral bending position lumbar AP views to early find cause.

남매에서 발생한 심한 용혈성 빈혈과 간경변증을 동반한 Wilson 병 2례

노영일,박동호,서우철,문경래,박상기,박영봉,이미숙,전호종 조선대학교 1995 The Medical Journal of Chosun University Vol.20 No.1

We experienced two case of Wilson's disease in female and male sibling. The first case was a 10 year-old female who showed jaundice. pancytopenia and cirrhotic change in liver biopsy. She died 10 days after diagnosis. The second case of 7 year-old male showed specific symptoms and revealed increased AST/ALT, low serun copper level and increased 24 hour urinary copper excretion level He is being treated with D-penicillamine and follow up examinations.

상부경추 아탈구를 동반한 사각근증후군 치험 1례

허광호 ( Kwang Ho Heo ),강지훈 ( Ji Hun Kang ),박영회 ( Young Hoi Park ),금동호 ( Dong Ho Keum ) 한방재활의학과학회 2006 한방재활의학과학회지 Vol.16 No.2

Objectives : This study was performed to evaluate the effect of acupuncture and chuna treatment on patient with Scaleneus Muscle Syndrome contain C2-C3 malposition. Methods : The patient was hospitalized Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dong-Guk University from 24th, February 2005 to 12th, May 2005, diagnosed as thoracic outlet syndrome and treated with acupuncture and so on. Results & Conclusions : Symptom of patient such as thoracic outlet syndrome was improved after acupuncture and chuna treatment. It is suggested that oriental medical treatment are effective in the Scaleneus Muscle Syndrome Contain C2-C3 Malposition.

경추성 두통에서 박동성 전기자장 치료의 효과 연구: 무작위 임상 연구

허광호 ( Kwang Ho Heo ),황형주 ( Hyung Joo Hwang ),박영회 ( Young Hoi Park ),금동호 ( Dong Ho Keum ) 한방재활의학과학회 2007 한방재활의학과학회지 Vol.17 No.3

Objectives:The study was performed to evaluate the effects of ElectroMagnetic Therapy treatment for patients suffering from cervicogenic headaches.Methods:After receiving medical examination at the Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dong-Guk University from November 1, 2006 to June 5, 2007, 34 patients with cervicogenic headache were devided into two groups(experimental vs control).Experimental group(n=18) was treated with both Pulsed ElectroMagnetic therapy(PEMT) and acupuncture, control group(n=16) was treated with acupuncture only. Visual Analogue Scale(VAS) was measured as a primary outcome measure, and was statistically analyzed using t-test.Results:Experimental group was effective than control group in pain intensity using VAS, because PEMT plus acupuncture was more effective than acupuncture only(p<0.05).Conclusions:The results indicate that the pulsed electromagnetic therapy is effective on treating cervicogenic headaches.