한번의 간질발작 후 혈청 Neuron-Specific Enolase 농도 변화

최승호,박영춘,이장준,임정근,김지언,이상도 啓明大學校 醫科大學 1999 계명의대학술지 Vol.18 No.4

An increase in neuron-specific enolase (NSE) levels in serum and CSF has been shown to be an useful marker of brain damage after stroke, global ischemia, and coma. We report the changes of serum NSE levels after seizure attacks in epileptic patients compared with the levels in normal controls and epileptic patients without seizure attack at least 7 days (epileptic controls). Twenty-four seizures were included in this study. Blood was drawn within 1 hour, at 12 hour, 24 hour, and 48 hour after seizure attack. Serum NSE levels were measured with radioimmunoassay. The mean NSE levels for normal controls and epileptic controls were 6.94 ng/ml and 7.46 ng/ml, respectively. There were significant increase in level of serum NSE measured within 1 hour after seizure attack in epileptics compared with the level in normal controls (15.10ng/ml versus 6.94ng/ml, p<0.05) and epileptic controls (15.10ng/ml versus 7.46ng/ml, p<0.05). Serum NSE measured at 12 hours after seizure also increased compared with normal controls (12.32ng/ml versus 6.94ng/ml, p<0.05) and epileptic controls (12.43ng/ml versus 7.46ng/ml, p<0.05). Between normal controls and epileptic controls, there were no significant difference in serum NSE levels. We conclude that serum NSE level was elevated in epileptic patients who had seizure attack within 12 hours. Serum NSE levels can be useful marker for seizure within 12 hours after onser. The elevated serum NSE level after single seizure attack may suggest that the brain was injured in single seizure.

Developmental Process and DNA Polymorphism of Rosette Gall in Aster scaber Thunb.

Doe-Seung Lee,Key-Zung Riu 한국토양비료학회 2001 한국토양비료학회 학술발표회 초록집 Vol.2001 No.5

쿠싱병의 임상적 고찰

김태승,이경미,이찬희,김영수,김동익,허갑범,이현철,이은직,김경래,조재식,이규창,정상섭,윤도흠 대한내분비학회 1993 Endocrinology and metabolism Vol.8 No.3

Cushing's syndrome caused by ACTH secreting pituitary adenoma or by corticotroph hyperplasia in referred to Cushing's disease. Since the original description of Cushing's disease by Harvey Cushing in 1932, lthere has been marked progression in the etiology, diagnosis and treatment of this disorder. Despite the progression, the diverse clinical, endocrinological and pathological features of Cushing's disease remin incompetely understood and there also remain many problems to be resolved about the diagnosis, treatment, and prognosis of Cushing's disease. Sixteen cases with Cushing's disease were evaluated in this study. The follwing results were obtained. 1) The patients consist of 12 females and 4 males and the age was ranged from 18 to 47 years. 2) Among 16 patients, 14 patients showed central obesity and buffalo hunp, 13 moon face. Among 12 female patients, 10 patients showed abnormalities of menstration. 3) Elevated levels and loss of diurnal variation of the serum cortisol were observed in all patients, and increased 24hr urine free cortisol in 11 out of 12 patients tested. Urinary cortisol excretion was not suppressed by low dose dexamethasone suppression test but suppressed by high dose dexamethasone test. 4) Computed tomogram and MRI revealed that 15 casese were micreadenoma and 1 was macroadenoma. 5) The modalties of treatment were TSA (11 cases), TSA with Radiotherapy (2 cases), and r-knife radiosurgery (3 cases). 6) During the follow up periods(2~31 months), 2 cases were recurred. In conclusion, 15 cases of 16 Cushing's disease were microadenoma. The standard dexamethasone suppression test was useful in the biochemical diagnosis, and inferior petrous venous sampling was also needed to clarify the localization of tumor which was not detected by MRI or CT scan.( J Kor Soc Endocrinol 8: 273~280, 1993).

뇌하수체 갑상선자극호르몬분비선종

임승길,김경래,정윤석,이경미,김동익,김현만,이현철,이은직,안광진,김덕희,윤도훔,박용구,김영수,정상섭,이규창,정우희,김태승,허갑범 대한내분비학회 1992 Endocrinology and metabolism Vol.7 No.4

TSH secreting pituitary adenoma is a rare disorder and represents about 1% of all pituitary adenomas. About 80 cases have so far been reported. We experienced four cases of TSH secreting pituitary adenoma. Three of them were macroadenomas and 1 was microadenoma. Serum TSH levels varied between 4.1~185.6 uIU/ml. The serum -subunit levels of two patients tested were slightly elevated (0.87, 0.96 mIU/ml). Serum TSH and -subunit response to TSH, dexamethasone, T3, bromocriptine, and octreotide were variable when studied. Three patients underwent transsphenoidal microsurgery. One of them received radiotherapy. They were cured. Treatment with octreotide in 1 patient who did not received surgery resulted in normalization of serum TSH and thyroid hormone levels and reduction in tumor size. (J Kor Soc Endocrinol 7:331~342, 1992)

Poster Session 2 / G-30 : Specific Interaction Between Phospholipase A2 And Annexin-1

(Doe Sun Na),(Seung Wook Kim),(Hae Jin Lee),(Yeo Jeong Kim) 한국생화학분자생물학회 (구 한국생화학회) 1999 생화학분자생물학회 춘계학술발표논문집 Vol.- No.-

심판막 치환술을 받은 환자의 만기 합병증으로 발생한 뇌졸중

이승환,이형,임정근,이상도,박영춘 대한신경과학회 1998 대한신경과학회지 Vol.16 No.4

갑상선자극호르몬분비성 뇌하수체 미세선종 1예

이경미,이은직,김경래,배희동,조현근,정재희,임승길,이현철,허갑범,윤도흠,김영수,정우희,김태승,김동익 대한내과학회 1993 대한내과학회지 Vol.44 No.1

저자등은 수년간 갑상선 기능항진증으로 치료받고 있었던 환자에서 혈청 호르몬검사 및 MRI로 뇌하수체 미세선종을 확인후, 경접형동 뇌하수체 종양 절제술을 통하여 TSH 분비성 뇌하수체 미세선종으로 확진된 1예를 문헌고찰과 함께 보고하는 바이다. Hyperthyroidism associated with TSH secreting pituitary adenoma is a rare disorder characterised by highserum T_4 and T_3 as well as elevated TSH level, especially the microadenoma has been demonstrated only in five cases. We experienced a case of 45 year old male with hyperthyroidism due to TSH secreting pituitary microadenoma. Transsphenoidal microsurgery resulted in the removal of a acidophilic microadenoma comprised mainly of thyrotropes revealed by immunocytochemistry. Postoperatively, serum TSH, T_4 and T_3 levels fell below normal limit. So we present this case of TSH secreting pituitary microadenoma with a literature review.

Translocation of Annexin I to the Nucleus by Epidermal Growth Factor in A549 Cells

Na, Doe Sun,Lee, Soo Ok,Kim, Seung Wook,Rhee, Hae Jin,Park, Young Min The Korea Science and Technology Center 1999 BMB Reports Vol.32 No.1

Annexin 1 (also called lipocortin 1), a 37-kDa member of the annexin family of proteins, has been implicated in the mitogenic signal transduction by epidermal growth factor (EGF). Annexin 1 is phosphorylated by the EGF signal, however, the role of annexin 1 in the EGF signal transduction is still unknown. To transduce extracellular signals into the intracellular targets, selective translocation of the signaling molecules to their targets would be necessary. In this study, we examined the subcellular locations of annexin 1 during EGF signal transduction. Treatment of A549 cells with EGF resulted in the translocation of cytoplasmic annexin 1 to the nucleus and perinuclear region as determined by Western blot and immunofluorescent staining. The nuclear translocation of annexin 1 was inhibited by tyrphostin AG 1478 and genistein, the inhibitors of EGF receptor kinase and downstream tyrosine kinases, respectively. Pretreatment of cells with cyclohexamide did not inhibit the nuclear translocation. The results suggest that nuclear translocation of annexin 1 is controlled by a series of kinase dependentevents in the EGF receptor signaling pathway and may be important in tranducing the signals by EGF.

Translocation of Annexin I to the Nucleus by Epidermal Growth Factor in A549 Cells

Kim, Seung Wook,Na, Doe Sun,Park, Young Min,Lee, Soo Ok,Rhee, Hae Jin 생화학분자생물학회 1997 BMB Reports Vol.32 No.1

Annexin I (also called lipocortin 1), a 37-kDa member of the annexin family of proteins, has been implicated in the mitogenic signal transduction by epidermal growth factor (EGF). Annexin I is phosphorylated by the EGF signal, however, the role of annexin I in the EGF signal transduction is still unknown. To transduce extracellular signals into the intracellular targets, selective translocation of the signaling molecules to their targets would be necessary. In this study, we examined the subcellular locations of annexin I during EGF signal transduction. Treatment of A549 cells with EGF resulted in the translocation of cytoplasmic annexin I to the nucleus and perinuclear region as determined by Western blot and immunofluorescent staining. The nuclear translocation of annexin I was inhibited by tyrphostin AG 1478 and genistein, the inhibitors of EGF receptor kinase and downstream tyrosine kinases, respectively. Pretreatment of cells with cyclohexamide did not inhibit the nuclear translocation. The results suggest that nuclear translocation of annexin I is controlled by a series of kinase dependent events in the EGF receptor signaling pathway and may be important in tranducing the signals by EGF.

학습컨설팅 역량 척도의 개발 및 타당화

이명숙(Lee Myung Sook),안도희(Ahn Doe hee),도승이(Do Seung Lee) 학습자중심교과교육학회 2015 학습자중심교과교육연구 Vol.15 No.9

본 연구는 학습컨설팅 역량 척도(LCCS: learning consultation competency scale) 를 개발하여 타당화를 하는데 목적이 있다. 본 연구에서는 학습컨설팅 관련 교육을 받는 학습클리닉 요원 228명이 참여하였다. 먼저, 학습컨설팅 역량 척도 개발을 위 하여 Dougherty(2000, 2013) 등의 연구와 국내외 학습과 학교기반 컨설팅 관련 연구 연구를 토대로 학습컨설팅 역량과 하위역량의 6 개념을 추출하였다. 6 개의 하위요 인은 컨설팅 계획과 대인관계 기술, 의사소통 기술, 컨설팅 기술, 학습기술, 평가 및 종결 기술, 지원 활동 및 윤리이며, 총 50 문항의 예비척도를 구축하였다. 예비척도 에 대한 탐색적 요인분석을 실시한 결과 요인 부하량이 .60이상인 총 44개 문항의 양호도를 확인하였으며, 학습컨설팅 계획과 대인관계 기술, 의사소통 기술, 컨설팅 기술, 학습 기술, 학습컨설팅 평가 및 종결 기술, 학습컨설팅 지원 기술, 윤리로 구분 되는 7개의 하위요인을 추출하였고, 7개 하위요인의 총 설명변량은 67.11%로 나타 났다. 다음으로 이렇게 개발된 최종 척도의 타당화 검증을 위해 구조방정식 모형을 활용한 확인적 요인분석과 신뢰도 분석을 하였다. 최종 LCCS의 전체 신뢰도 Cronbach α는 .95로 나타났으며, 하위요인들의 신뢰도 범위는 .85에서 .94로 양호한 수준을 보였고, 최종 LCCS의 요인구조 적합도 지수인 X2/(df), CFI, TLI, RMSEA, SRMR가 수용 가능한 기준치를 충족시켜 구조적으로 적합한 모형으로 판명되었다. 본 척도개발의 시사점과 추후연구를 위한 논의점을 제시하였다. The purpose of this study was to develop and to validate the learning consultation competency scale(LCCS). A total 228 consultants who work at the Offices of Education-affiliated learning clinic in Chungcheong and Gyeongsang regions participated in this study. First, a preliminary LCCS of 50 items consist with 6 sub-factors such as consultation plans and interpersonal skills, communication skills, consultation skills, learning skills, consultation evaluation and termination skill, supporting activity and ethics were extracted based on the studies of Dougherty(2000, 2013), Brown, Pryzwansky, and Schulte(2001), and Kampwirth(2010). As a result of the exploratory factor analysis, 44 items consisting with 7 sub-factors were confirmed as valid meeting criteria of factor loadings above .60. These 7 sub-factors were labeled as consultation plans and interpersonal skills, communication skills, consultation skills, learning skills, consultation evaluation and termination skill, consultation supporting skill, and consultant ethics. Second, confirmatory factor analysis utilizing structural equation model was performed in order to validate the final LCCS. As a result, the model fit indices of X2/(df), CFI, TLI, RMSEA, and SRMR were met the sufficient fit criteria, the overall reliability Cronbach α for the LCCS was .951 and the reliabilities of 7 sub-factors ranged from .851 to .936, and the final LCCS can serve as valid and reliable measure for the leaning consultation competencies.