Detection of Listeria Species by Conventional Culture-Dependent and Alternative Rapid Detection Methods in Retail Ready-to-Eat Foods in Turkey

( Emine Dincer ) 한국미생물생명공학회 2024 Journal of microbiology and biotechnology Vol.34 No.2

Foodborne pathogens, like Listeria monocytogenes, continue to inflict substantial financial losses on the food industry. Various methods for detecting Listeria in food have been developed and numerous studies have been conducted to compare the different methods. But, in recent years, new Listeria species have been identified, and currently the genus comprises 26 species. Therefore, it would be a more accurate approach to re-evaluate existing detection methods by considering new species. The present investigation involved the analysis of 42 ready-to-eat (RTE) foods, encompassing a variety of food categories, such as mezes, salads, dairy products, and meat products, with the aim of ascertaining the presence of Listeria. Among the traditional culture-dependent reference methods, the ISO 11290 method was preferred. The process of strain identification was conducted with the API Identification System. Furthermore, to ascertain the existence of L. monocytogenes and Listeria spp., the samples underwent additional analysis employing the VIDAS Immunoassay System, ELISA, and RT-PCR methodologies. Thus, four alternative approaches were employed in this study to compare not only the different methods used to determine Listeria while taking into account the newly identified Listeria species, but also to assess the compliance of retail RTE food items with microbiological criteria pertaining to the genus Listeria. Based on the conducted analyses, L. monocytogenes was conclusively determined to be present in one sample. The presence of Listeria spp. was detected in 30.9% of the samples, specifically in Turkish cig kofte, sliced salami, and salads.

Stoichiometry of Pt Submonolayer Deposition via Surface-Limited Redox Replacement Reaction

Gokcen, Dincer,Bae, Sang-Eun,Brankovic, Stanko R. The Electrochemical Society 2010 Journal of the Electrochemical Society Vol.157 No.11

Letter to the Editor : Posttraumatic Ectopic Nail: A Usual Manifestation and Treatment of an Unusual Anomaly

( Ercan Caliskan ),( Didem Dincer ),( Gurol Acikgoz ),( Erol Koc ) 대한피부과학회 2014 Annals of Dermatology Vol.26 No.6

Thermodynamic Analysis and Performance Assessment of a Cascade Active Magnetic Regenerative Refrigeration System

HADI GANJEHSARABI,IBRAHIM DINCER,ALI GUNGOR 대한설비공학회 2013 International Journal Of Air-Conditioning and Refr Vol.21 No.3

In the present study, a thermodynamic model is proposed to analyze and assess the performance,through energy and exergy, of a cascade active magnetic regenerative (AMR) refrigerator operation a regenerative Brayton cycle. This cascade refrigeration system works with GdxTb1x alloys as magnetic materials where the composition of the alloy varies for di®erent stages. In this model, the heat transfer °uid considered is a water– glycol mixture (50% by weight). The refrigeration capacity,total power consumption, coe±cients of performance (COP), exergy e±ciency and exergy destruction rate of a cascade AMR refrigeration (AMRR) system are determined. To understand the system performance more comprehensively, a parametric study is performed to investigate the e®ects of several important design parameters on COP and exergy e±ciency of the system.

Preparation and Characterization of Viburnum Opulus Containing Electrospun Membranes as Antibacterial Wound Dressing

Adile Yuruk,Sevil Dincer Isoglu,Ismail Alper Isoglu 한국섬유공학회 2023 Fibers and polymers Vol.24 No.11

Herein, we fabricated polycaprolactone/gelatin electrospun membranes possessing different amounts of Viburnum Opulus extract (0, 25, 35, 50%, w/v) as an antibacterial wound dressing. We investigated chemical, morphological, physical, and mechanical properties as well as in vitro degradation behavior of the electrospun membranes. The antibacterial activities of membranes were evaluated against gram-positive Staphylococcus aureus (S. aureus) and gram-negative Escherichia coli (E. coli). The membranes containing Viburnum Opulus exhibited excellent antibacterial activity with the formation of inhibition zones of 25 mm to 36 mm against Escherichia coli and 14 mm to 25 mm against Staphylococcus aureus. The fiber diameters rose from 591 to 1222 nm after adding Viburnum Opulus extract. The extract-containing membranes displayed superior swelling, cell viability, and proliferation properties to neat membranes. Our results showed that the polycaprolactone/gelatin electrospun membranes containing Viburnum Opulus could be a suitable material for wound dressing applications.

Modeling of concrete containing steel fibers: toughness and mechanical properties

Ismail H. Cagatay,Riza Dincer 사단법인 한국계산역학회 2011 Computers and Concrete, An International Journal Vol.8 No.3

In this study, effect of steel fibers on toughness and some mechanical properties of concrete were investigated. Hooked-end steel fibers were used in concrete samples with three volume fractions (vf) of 0.5%, 0.75% and 1% and for two aspect ratios (l/d) of 45 and 65. Compressive and flexural tensile strength and modulus of elasticity of concrete were determined for cylindrical, cubic and prismatic samples at the age of 7 and 28 days. The stress-strain curves of standard cylindrical specimens were studied to determine the effect of steel fibers on toughness of steel-fiber-reinforced concrete (SFRC). In addition, the relationship between compressive strength and the flexural tensile strength of SFRC were reported. Finally, a simple model was proposed to generate the stress-strain curves for SFRC based on strains corresponding to the peak compressive strength and 60% of peak compressive stress. The proposed model was shown to provide results in good correlation with the experimental results.

Vitamin D Levels in Patients with Breast Cancer: Importance of Dressing Style

Alco, Gul,Igdem, Sefik,Dincer, Maktav,Ozmen, Vahit,Saglam, Sezer,Selamoglu, Derya,Erdogan, Zeynep,Ordu, Cetin,Pilanci, Kezban Nur,Bozdogan, Atilla,Yenice, Sedef,Tecimer, Coskun,Demir, Gokhan,Koksal, G Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.3

Background: Vitamin D deficiency is a potentially modifiable risk factor that may be targeted for breast cancer (BC) prevention. It may also be related to prognosis after diagnosis and treatment. The aim of our study was to determine the prevalence of vitamin D deficiency as measured by serum 25-hydroxy vitamin D (25-OHD) levels in patients with BC and to evaluate its correlations with life-style and treatments. Materials and Methods: This study included 186 patients with stage 0-III BC treated in our breast center between 2010-2013. The correlation between serum baseline 25-OHD levels and supplement usage, age, menopausal status, diabetes mellitus, usage of bisphosphonates, body-mass index (BMI), season, dressing style, administration of systemic treatments and radiotherapy were investigated. The distribution of serum 25-OHD levels was categorized as deficient (<10ng/ml), insufficient (10-24 ng/ml), and sufficient (25-80 ng/ml). Results: The median age of the patients was 51 years (range: 27-79 years) and 70% of them had deficient/insufficient 25-OHD levels. On univariate analysis, vitamin D deficiency/insufficiency was more common in patients with none or low dose vitamin D supplementation at the baseline, high BMI (${\geq}25$), no bisphosphonate usage, and a conservative dressing style. On multivariate analysis, none or low dose vitamin D supplementation, and decreased sun-exposure due to a conservative dressing style were found as independent factors increasing risk of vitamin D deficiency/insufficiency 28.7 (p=0.002) and 13.4 (p=0.003) fold, respectively. Conclusions: The prevalence of serum 25-OHD deficiency/insufficiency is high in our BC survivors. Vitamin D status should be routinely evaluated for all women, especially those with a conservative dressing style, as part of regular preventive care, and they should take supplemental vitamin D.

Effect of Pulsed Electromagnetic Field on MMP-9 and TIMP-1 Levels in Chondrosarcoma Cells Stimulated with IL-1β

Caliskan, Serife Gokce,Bilgin, Mehmet Dincer,Kozaci, Leyla Didem Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.7

Chondrosarcoma, the second most common type of bone malignancy, is characterized by distant metastasis and local invasion. Previous studies have shown that treatment by pulsed electromagnetic field (PEMF) has beneficial effects on various cancer cells. In this study, we investigated the effects of PEMF applied for 3 and 7 days on the matrix metalloproteinase (MMP) levels in chondrosarcoma SW1353 cells stimulated with two different doses of $IL-1{\beta}$. SW1353 cells were treated with (0.5 and 5 ng/ml) $IL-1{\beta}$ and PEMF exposure was applied either 3 or 7 days. MMP-9 and TIMP-1 levels were measured in conditioned media by enzyme-linked immunosorbent assay. The results were relative to protein levels. Statistical analyses were performed using one-way analysis of variance (ANOVA). P<0.05 was considered significant. PEMF treatment significantly decreased MMP-9 protein levels in human chondrosarcoma cells stimulated with 0.5 ng/ml $IL-1{\beta}$ at day 7, whereas it did not show any effect on cells stimulated with 5 ng/ml $IL-1{\beta}$. There was no significant change in TIMP-1 protein levels either by $IL-1{\beta}$ stimulation or by PEMF treatment. The results of this study showed that PEMF treatment suppressed $IL-1{\beta}$-mediated upregulation of MMP-9 protein levels in a dual effect manner. This finding may offer new perspectives in the therapy of bone cancer.

Posttreatment Effects of Olea Europaea L. Leaf Extract on Carbon Tetrachloride-Induced Liver Injury and Oxidative Stress in Rats

Derya Ustuner,Emine Colak,Murat Dincer,Neslihan Tekin,Dilek Burukoglu Donmez,Fahrettin Akyuz,Ertugrul Colak,Umut Kerem Kolac,Emre Entok,Mehmet Cengiz Ustuner 한국식품영양과학회 2018 Journal of medicinal food Vol.21 No.9

The aim of this study is to examine the therapeutic effects of Olea europaea L. leaf extract on carbon tetrachloride (CCl4)-induced liver damage in rats. In the experiments, 3- to 4-month-old 28 male Sprague–Dawley rats were divided into four groups: control, O. europaea leaf extract, CCl4, and curative. The CCl4 and curative groups received CCl4 (0.2 mL/kg) intraperitoneally for 10 days to form hepatic injury. O. europaea (80 mg/kg) leaf extract was given orally to the curative group dissolved in distilled water the following 14 days. Hepatic and antioxidant enzyme levels, p53, caspase 3, lipid peroxidation marker malondialdehyde (MDA), and also DNA fragmentation levels were determined to establish oxidative stress in hepatic cell damage and its consequences. After formation of liver damage, oral administration of the O. europaea significantly reduced CCl4-induced elevations of serum alkaline phosphatase, aspartate aminotransferase and alanine aminotransferase levels (P < .001), MDA levels of both blood (P < .001) and liver tissues (P < .001), DNA fragmentation (P < .001), p53 (P < .001), and caspase 3 (P < .001) levels of liver tissues. Also this administration in curative group significantly increased CCl4-induced reductions of superoxide dismutase (SOD) (P < .001) and catalase (CAT) (P < .001) activity of blood samples and decreased SOD (P < .001) and CAT (P < .05) activity observed in liver tissue curative groups compared with CCl4 curative group. In CCl4 group, liver tissue samples exhibited remarkable damage because of CCl4 and reduction of these damages were observed in the curative group. Our results showed that O. europaea leaf extract was effective in reducing hepatic damage caused by CCl4 by reducing lipid peroxidation, regulating antioxidant enzymes, and minimizing DNA damage.

Identification and Saturable Nature of Signaling Pathways Induced by Metreleptin in Humans: Comparative Evaluation of In Vivo, Ex Vivo, and In Vitro Administration

( Hyun-seuk Moon ),( Joo Young Huh ),( Fadime Dincer ),( Benjamin E. Schneider ),( Per-olof Hasselgren ),( Christos S. Mantzoros ) 전남대학교 약품개발연구소 2015 약품개발연구지 Vol.24 No.-

Signaling pathways activated by leptin in metabolically important organs have largely been studied only in animal and/or cell culture studies. In this study, we examined whether leptin has similar effects in human peripheral tissues in vivo, ex vivo, and in vitro and whether the response would be different in lean and obese humans. For in vivo leptin signaling, metreleptin was administered and muscle, adipose tissue, and peripheral blood mononuclear cells were taken for analysis of signal activation. Experiments were also done ex vivo and with primary cultured cells in vitro. The signal activation was compared between male versus female and obese versus lean humans. Acute in vivo, ex vivo, and/or in vitro metreleptin administration similarly activated STAT3, AMPK, ERK1/2, Akt, mTOR, NF-κB, and/or IKKα/β without any differences between male versus female and obese versus lean subjects. All signaling pathways were saturable at ~30-50 ng/mL, consistent with the clinical evidence showing no additional effect(s) in obese subjects who already have high levels of leptin. Our data provide novel information on downstream effectors of metreleptin action in humans that may have therapeutic implications.