Pre-Seeding of Simple Electrospun Scaffolds with a Combination of Endothelial Cells and Fibroblasts Strongly Promotes Angiogenesis

Dikici Serkan,Claeyssens Frederik,MacNeil Sheila 한국조직공학과 재생의학회 2020 조직공학과 재생의학 Vol.17 No.4

Background: Introduction of pro-angiogenic cells into tissue-engineered (TE) constructs (prevascularisation) is a promising approach to overcome delayed neovascularisation of such constructs post-implantation. Accordingly, in this study, we examined the contribution of human dermal microvascular endothelial cells (HDMECs) and human dermal fibroblasts (HDFs) alone and in combination on the formation of new blood vessels in ex-ovo chick chorioallantoic membrane (CAM) assay. Methods: Poly-3-hydroxybutyrate-co-3-hydroxyvalerate (PHBV) and polycaprolactone (PCL) were first examined in terms of their physical, mechanical, and biological performances. The effect of gelatin coating and co-culture conditions on enhancing endothelial cell viability and growth was then investigated. Finally, the angiogenic potential of HDMECs and HDFs were assessed macroscopically and histologically after seeding on simple electrospun PHBV scaffolds either in isolation or in indirect co-culture using an ex-ovo CAM assay. Results: The results demonstrated that PHBV was slightly more favourable than PCL for HDMECs in terms of cell metabolic activity. The gelatin coating of PHBV scaffolds and co-culture of HDMECs with HDFs both showed a positive impact on HDMECs viability and growth. Both cell types induced angiogenesis over 7 days in the CAM assay either in isolation or in co-culture. The introduction of HDMECs to the scaffolds resulted in the production of more blood vessels in the area of implantation than the introduction of HDFs, but the co-culture of HDMECs and HDFs gave the most significant angiogenic activity. Conclusion: Our findings showed that the in vitro prevascularisation of TE constructs with HDMECs and HDFs alone or in co-culture promotes angiogenesis in implantable TE constructs.

Comparision of the Limberg flap with the V-Y flap technique in the treatment of pilonidal disease

Altintoprak Fatih,Dikicier Enis,Arslan Yusuf,Ozkececi Taner,Akbulut Gokhan,Dilek Osman Nuri 대한외과학회 2013 Annals of Surgical Treatment and Research(ASRT) Vol.85 No.2

Purpose: In this study, we investigated whether there is a factor that can aid determi nation of the preferred technique by comparing the early and late results of two different surgical techniques for the treatment of pilonidal sinus. Methods: The medical records of 176 patients in whom the Limberg flap (LF) or V-Y flap techniques were applied for reconstruction after the excision were evaluated retrospectively. Results: The development rates of postoperative hematoma, wound separation, wound infection, and seroma were 2.8%, 5.1%, 5.6%, and 6.3%, respectively, while total flap necrosis was not observed in any patient. Return to daily activities was achieved after a mean of 17.1 days (13 to 21 days) days in the LF group and 32.7 days (18 to 47 days) in the V-Y flap group. During the average follow-up of 65 months (36 to 110 months), nine patients (5.1%) developed recurrent disease. There was no difference between the two groups with respect to early surgical complications (P = 0.286) or disease recurrence (P = 0.094), whereas the resumption of daily activities was longer in patients with a V-Y flap (P < 0.001). Conclusion: The early postoperative and long-term results of the LF and V-Y flap techniques for the treatment of pilonidal sinus were similar. Because the resumption of daily activities at work is achieved later in patients undergoing the V-Y flap compared with the LF technique, patients’ employment (or position in working life) must be considered when determining the most appropriate surgical technique.

Investigation of the Mechanical, Electronic and Phonon Properties of X2ScAl (X = Ir, Os, and Pt) Heusler Compounds

Arikan Nihat,Ocak Hamza Yaşar,Dikici Yıldız Gökçen,Yıldız Yasin Göktürk,Ünal Rahmi 한국물리학회 2020 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.76 No.10

In the present study, the second-order elastic constants and the electronic band structures of the X2ScAl (x = Ir, Os, and Pt) compounds crystallized in the L21 phase were calculated separately by using the ab-initio density functional theory. According to the results for the second-order elastic constants, these compounds met the Born mechanical stability criteria. Also, according to the Pugh criteria, they were found to have a ductile structure and to show anisotropic behavior. The microhardneses of the compounds were between 2 and 14 GPa, and the highest hardness was found in the Ir2ScAl (14.290 GPa) compound. In addition, the energy band structures of these compounds were calculated, and the crystals were found to have a metallic bond structure. All the computed data were compared with previously calculated results obtained with different methods. According to the findings obtained in the present study, in terms of its mechanical and electronic behaviors, Ir2ScAl was found to have better physical properties than Os2ScAl and Pt2ScAl. The phonon dispersion curves and their corresponding total and projected densities of states were investigated for the first time by using a linear-response approach in the context of density functional perturbation theory. The frequencies of the optical phonon modes of all compounds at the Γ point were 4.767, 7.504 and 9.271 THz for Ir2ScAl, 2.761, 7.985 and 9.184 THz for Os2ScAl and 2.012, 5.6952 and 8.118 THz for Pt2ScAl. The heat capacity Cv at constant volume versus temperature was calculated using a quasi-harmonic approach and the results are discussed.

Women's Perceptions and Attitudes about Cervical Cancer in Turkey: Kato's Device as an Alternative to the Pap Smear

Sahin, Mustafa Kursat,Sahin, Gulay,Dikici, Mustafa Fevzi,Igde, Fusun Artiran,Yaris, Fusun Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.2

Aims: To determine the status of women's perception and attitudes about cervical cancer and their thoughts on Kato's self-sampling device. Materials and Methods: This descriptive research was conducted between July-December 2012 with a study populationof married women older than 18 years. Results: A total of 246 women volunteered, with a mean age of $34.9{\pm}9.22$ (19-52). In the last year, 52.0% had been examined by a gynecologist to address a complaint or for a periodic health check. Of the 118 who had not had a gynecological examination, 42.4% indicated negligence, 26.3% stated the reason was no complaint, 14.4% feared they might receive bad results from the examination, and 8.5% stated that were shy or embarrassed. Of all of the women, 35.0% answered that they had information about cervical cancer, and 0.7% had heard about the HPV vaccine. No one in their families had received the vaccine. Of the women, 28.5% had had a Pap smear, and 71.4% of those had normal results. Of those who had never had a Pap smear, 47.2% had never heard about the test; 18.8% explained the reason for not having a test as shyness or embarrassment with a male doctor. None of the women had heard about Kato's device. Once the women were informed, 73.6% expresseed interest in use; 51.9% answered they could use it and not be embarrassed, 30.9% would use it because they did not have to see a doctor, and 17.1% would use it as it allows them to take a smear in all conditions and whenever they want. Of the women, 60.0% thought that they could not successfully use Kato's device; 40.0% thought that a smear should be taken by a doctor. Conclusions: Most of the women in our population had never had a Pap smear and wanted to use the Kato's device.

ZnO nanostructures for photocatalytic degradation of methylene blue: effect of different anodization parameters

Oksuz Ahmet Emrecan,Yurddaskal Metin,Kartal Ugur,Dikici Tuncay,Erol Mustafa 한국세라믹학회 2022 한국세라믹학회지 Vol.59 No.6

In this paper, the photocatalytic activity of ZnO nanostructures formed by anodization method with diff erent parameters was investigated. The synthesis of ZnO nanostructures with diff erent morphology by varying anodic oxidation parameters containing electrolytes, molarity, voltage, and duration was analyzed. ZnO nanostructures were prepared through diff erent parameters consisting of six samples. The produced ZnO nanostructures were investigated by using X-ray diff raction, scan- ning electron microscopy, diff use refl ectance spectroscopy, and UV–Vis spectrophotometer. It was found that the morphology of ZnO structures was formed as nanorods, needle-like, fl ower-like, heterogeneous, and homogeneous of mixed structures. ZnO nanostructures were identified by matching X-Ray diffraction peaks due to the international center for diffraction data database. Experiments on photocatalytic degradation of methylene blue demonstrated that the photocatalytic activity of ZnO samples. The best photocatalytic performance was observed by the sample anodized for an hour in 0.05 M of KHCO 3 electrolytes with 40 V electrical potential. It was observed that the removal of methylene blue increased 3 times (photocatalytic degradation efficiency ~ 31% for methylene blue vs ~ 90% by the best sample) thanks to the obtained ZnO nanostructured photocatalysts. The results showed that an increment of the voltage has a significant effect on the photocatalytic activity of ZnO while keeping other parameters including molarity, time, and electrolyte type constant.

Giant intra-abdominal cystic lymphangioma

Fatih Altintoprak,Mustafa Yener Uzunoglu,Kayhan Ozdemir,Enis Dikicier,Zeynep Kahyaoglu 대한내과학회 2019 The Korean Journal of Internal Medicine Vol.34 No.3

Disorders Center, Fujieda Heisei Memorial Hospital, Fujieda, Shizuoka, Japan

Turgut Akgül,Kerim Sarıyılmaz,Murat Korkmaz,Okan Özkunt,Özcan Kaya,Fatih Dikici 대한척추외과학회 2018 Asian Spine Journal Vol.12 No.1

Study Design: Retrospective analysis of adolescent idiopathic scoliosis. Purpose: This study aimed to investigate the influence of distinct distal fusion levels on spinopelvic parameters in patients with adolescent idiopathic scoliosis (AIS) who underwent posterior instrumentation and fusion surgery. Overview of Literature: The distal fusion level selection in treatment of AIS is the one of milestone to effect on surgical outcome. Most of the paper focused on the coronal deformity correction and balance. The literature have lack of knowledge about spinopelvic changing after surgical treatment and the relation with distal fusion level. We evaluate the spinopelvic and pelvic parameter alteration after fusion surgery in treatment of AIS. Methods: A total of 100 patients with AIS (88 females and 12 males) were retrospectively reviewed. Patients were assigned into the following three groups according to the distal fusion level: lumbar 2 (L2), lumbar 3 (L3), and lumbar 4 (L4). Using a lateral plane radiograph of the whole spine, spinopelvic angular parameters such as thoracic kyphosis (TK), lumbar lordosis (LL), pelvic incidence (PI), sacral slope (SS), and pelvic tilt (PT) were radiologically assessed. Results: The mean age was 15±2.4 years, and the mean follow-up period was 24.27±11.69 months. Regarding the lowest instrumented vertebra, patients were categorized as follows: 30 patients in L2 (group 1), 40 patients in L3 (group 2), and 30 patients in L4 (group 3). TK decreased from 36.60±13.30 degrees preoperatively to 26.00±7.3 degrees postoperatively in each group (p =0.001). LL decreased from 52.8±9.4 degrees preoperatively to 44.30±7.50 degrees postoperatively (p =0.001). Although PI showed no difference preoperatively among the groups, it was statistically higher postoperatively in group 3 than in the other groups (p <0.05). In addition, in group 2, PI decreased from 50.60±8.74 degrees preoperatively to 48.00±6.84 degrees postoperatively (p =0.027). SS decreased from 35.20±6.40 degrees preoperatively to 33.40±5.80 degrees postoperatively (p =0.08, p >0.05). However, mean SS was significantly higher in group 3 (p =0.042, p <0.05). PT decreased from 15.50±7.90 degrees preoperatively to 15.2±7.10 degrees postoperatively. The positive relationship (28.5%) between LL and PI measurements was statistically significant (r =0.285; p =0.004, p <0.01). Furthermore, the positive relationship (36.5%) between LL and SS measurements was statistically significant (r =0.365; p =0.001, p <0.01). Conclusions: When the distal instrumentation level in AIS surgery is below L3, a significant change in PT and SS (pelvic parameters) is anticipated.