http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
In vitro Meristem Tip Culture of Rosa hybrida
Debasis Chakrabarty,Subodh Kumar Datta 한국원예학회 2006 Horticulture, Environment, and Biotechnology Vol.47 No.2
A micropropagation method for Rosa hybrida cv. Contempo and Mrinalini is presented using shoot tip proliferation onto Murashige and Skoog (MS) medium with 0.2 ㎎ㆍL?¹ indole-3-acetic acid (IAA) along with different concentrations of 6-benzylaminopurine (BAP). Percent of explant producing multiple shoots, number of shoots per responded explant and growth of shoots varied with BAP concentration. The optimum results were recorded in 2 ㎎ㆍL?¹ BA. Addition of AgNO₃ in the medium reduced leaf abscission of the in vitro developed shoots. The regenerated plants were transferred to rooting medium (×1/4 MS with 0.1 ㎎ㆍL?¹ IAA, 0.1 ㎎ㆍL?¹ indole-3-butyric acid, 30 gㆍL?¹ sucrose, and 0.6% agar). The highest percentage of root formation was observed in ‘Contempo’ (57.1%), whereas very low percentage of rooting (16.6%) was observed in ‘Mrinalini’. When rooting medium was supplemented with different concentrations of activated charcoal, increase in rooting percentage with increasing the concentration was recorded in the case of ‘Mrinalini’. At the end of 21-28 days, however, the shoots were deteriorated due to yellowing of leaflets. When the plantlets were rooted in liquid medium, on the contrary, the percentage of yellowing leaflets was almost zero. The resulting plantlets were acclimatized and grown in potted soil where they developed normally.
Lian, Mei-Lan,Debasis Chakrabarty,Paek, kee-Yoeup 한국식물학회 2002 Journal of Plant Biology Vol.45 No.4
We have established cell-suspension cultures of mountain ginseng (Panax ginseng C A. Mayer), and have attempted to increase the yield of saponin by manipulating our processing method and culturing factors (e.g., media strengths; the presence of plant growth regulators or sucrose; ratios of NO_3^+/NH_4^-). Maximum biomass yield was obtained in media containing 2,4-D. However, saponin productivity was much higher in a medium comprising either IBA or NAA; 7.0 ㎎/L IBA was optimal for promoting both cell growth (10.0 g/L dry weight) and saponin production (7.29 ㎎/g DW total ginsenoside). Although the addition of cytokinins (BA and kinetin) did not affect cell growth, the level of saponin (particularly in the Rb group) was enhanced when the media were supplemented with either 0.5 ㎎/L BA or 0.5 ㎎/L kinetin. Half- and full-strength MS media were equally suitable for inducing both biomass as well as saponin production. We also investigated the effect of various concentrations of sucrose and nitrogen, and found that 30 g/L sucrose enhanced biomass yield as well as saponin content However, further increases (i.e., up to 70 g/L) led to a decrease in saponin accumulation and biomass production. Maximum growth and saponin productivity were reported from treatments with an initial nitrogen concentration of 30 mM. In general, the amount of saponin increased when the test media had high NO_3^+/NH_4^- ratios; in fact, saponin production was greatest when nitrate was the sole nitrogen source.
Micropropagation of an Endangered Jewel Orchid (Anoectochilus formosanus) Using Bioreactor System
Rong Zhe Wu,Debasis Chakrabarty,Eun Joo Hahn,Kee Yoeup Paek 한국원예학회 2007 Horticulture, Environment, and Biotechnology Vol.48 No.6
We have developed a simple protocol for in vitro mass propagation of Anoectochilus formosanus using an automated low cost bioreactor system by a two-step culture. In the first step nodal and shoot tip cultures in gelled based media, N?-benzyladenine (BA) at 2 mgㆍL?¹ produced more shoots and higher weight than 2-isopentanyladenine (2iP) or kinetin (Kin). The shoot tips were also cultured in 3 l balloon type bubble bioreactor (BTBB) with different aeration volumes. Shoot proliferation and growth was the most effective when supply volume was 0.06 vvm. However, shoots developed in the bioreactor showed slow growth as well as small leaves. In the second step, shoots were transferred to 3 L BTBB containing 0.75 L Hyponex (20-20-20) 1 gㆍL?¹ + (6.5-4.5-19) 1 gㆍL?¹ medium with 2 gㆍL?¹ peptone, 3% sucrose, 0.5 gㆍL?¹ granular activated charcoal and different culture condition for shoot elongation and rooting. Comparative studies between gelled and bioreactor culture (continuous immersion [with or without net] and temporary immersion in liquid media using ebb and flood) revealed that shoot length was more efficient in gelled culture. However, the growth of plantlets was also higher in continuous immersion (with net) bioreactor culture. The effect of inoculation density on shoot growth during bioreactor culture was studied and maximum response was recorded when there were 60-90 inoculums per bioreactor included. Regenerated shoots with well developed roots were successfully acclimatized and transferred to greenhouse.
Yaser Hassan Dewir,Debasis Chakrabarty,Sun Ja Kim,Eun Joo Hahn,Kee Yoeup Paek 한국원예학회 2005 Horticulture, Environment, and Biotechnology Vol.46 No.6
The influences of light quality on growth of Euphorbia millii and shoot proliferation of Spathiphyllum cannifolium were studied in vitro and responses were compared with explants grown under fluorescent light (16 h per day). Fresh weight, dry weight, and leaf per plantlets were greater in blue light-emitting diode (LED) treatment in E. millii. Plantlet height was higher in red as well as in blue treatment than that of the other treatments. However, chlorophyll and carotenoid contents were highest in blue light and lowest in red as well as in red plus far-red LED treatments. Among the LED treatments, blue plus far-red light inhibited the growth of E. millii, where as blue light stimulated growth in vitro. In this study, the effect of different light quality using LED and white fluorescent lamps (as control) on shoot proliferation and growth of S. cannifolium in vitro were also investigated. Shoot proliferation as well as fresh and dry weights were significantly increased under red plus far-red or red LED.