몽골 <며느리 고르기>형 민담 연구

Davaa Uugantuya 숙명여자대학교 한국어문화연구소 2012 한국어와 문화 Vol.12 No.-

Preliminary study to determine the optimal conditions for the simultaneous complexation of siRNA and plasmid DNA

DAVAA ENKHZAYA,안익현,강봉석,이상은,명창선,박정숙 한국약제학회 2013 Journal of Pharmaceutical Investigation Vol.43 No.6

We investigated the complexation ratio of siRNA and plasmid DNA combined with Lipofectamine for the effective delivery of genetic materials. First, the amount of Lipofectamine was varied with a fixed amount of DNA or siRNA to determine in which proportions they would form an optimal combination. Finally, to investigate the effect of DNA or siRNA on the co-complexation of both DNA and siRNA, the co-complex of DNA and siRNA was prepared at the various ratios with a fixed amount of DNA. All complexation was confirmed by gel retardation of DNA or siRNA on agarose gels. The effects of siRNA complexes on mRNA expression from plasmid DNA were explored post-transfection, while the influence of plasmid DNA complexes on the transfection of siRNA was determined in GFP-expressing H4IIE cells. The complex between DNA and Lipofectamine was formed at a weight ratio of 0.8:1, whereas the light band of siRNA/Lipofectamine disappeared at a weight ratio of 4:1. When the amounts of DNA, siRNA, and the mixture were fixed, the optimal ratio of nucleic acids and Lipofectamine in our composition was 110:80:350 (ng). Confocal images and flow cytometry showed that inhibition of GFP expression by siRNA was not interfered with by co-complexed plasmid DNA. Moreover, mRNA expression of adiponectin was not hampered by the addition of siRNA; rather, it was increased. Thus, co-complexation of siRNA and plasmid DNA may have a synergistic effect on delivery of the therapeutic gene and siRNA.

Formulation Parameters Influencing the Physicochemical Characteristics of Rosiglitazone-loaded Cationic Lipid Emulsion

DAVAA ENKHZAYA,박정숙 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.7

To enhance the solubility of rosiglitazone, rosiglitazone-loaded cationic lipid emulsion was formulated using cationic lipid DOTAP, DOPE, castor oil, tween 20, and tween 80. The formulation parameters in terms of droplet size were optimized focused on the effect of the cationic lipid emulsion composition ratio on drug encapsulating efficiency, in vitro drug release, and cellular uptake of the rosiglitazone-loaded emulsion. Droplet sizes of a blank cationic emulsion and a rosiglitazone-loaded cationic emulsion ranged between 195-230 nm and 210-290 nm, respectively. The encapsulation efficiency of the rosiglitazone-loaded emulsion was more than 90%. The rosiglitazone-loaded cationic emulsion improved in vitro drug release over the drug alone and showed a much higher cellular uptake than rosiglitazone alone. Moreover, drug loading in cationic emulsions increased cellular uptake of rosiglitazone in insulin-resistant HepG2 cells more than the normal HepG2 cells. Taken together, these results indicate that cationic lipid emulsions could be a potential delivery system for rosiglitazone and could enhance its cellular uptake efficiency into target cells.

Complexation of Adiponectin-encoding Plasmid DNA with Rosiglitazone-loaded Cationic Liposomes

Davaa, Enkhzaya,Jeong, Ui-Hyeon,Shin, Baek-Ki,Choi, Soon-Gil,Myung, Chang-Seon,Park, Jeong-Sook The Korean Society of Pharmaceutical Sciences and 2010 Journal of Pharmaceutical Investigation Vol.40 No.6

To enhance therapeutic effects of insulin-sensitizing adipokine, ADN gene and potent agonists, rosiglitazone for the $PPAR{\gamma}$, cationic liposomes as non-viral vectors were formulated. The particle size and zeta potential of drug loaded and unloaded cationic liposomes were investigated. The complex formation between cationic liposomes and negatively charged plasmid DNA was confirmed and the protection from DNase was observed. In vitro transfection was investigated in HepG2, HeLa, and HEK293 cells by mRNA expression of ADN. Encapsulation efficacy of rosiglitazone-loaded liposomes was determined by UV detection. Particle sizes of cationic liposomes were in the range of 110-170 nm and those of rosiglitazone-loaded cationic liposomes were in the range of 130-180 nm, respectively. Gel retardation of complexes indicated that the complex was formed at weight ratios of cationic lipid to plasmid DNA higher than 20:1. Both complexes protected plasmid DNA from DNase either drug free or drug loading. Encapsulation efficiency of rosiglitazone-loaded emulsion was increased by drug dose. The mRNA expression levels of ADN were dose-dependently increased in cells transfected with plasmid DNA. Therefore, cationic liposomes could be potential co-delivery system for drug and gene.

MT1-MMP Responsive Doxorubicin Conjugated Poly(lactic-<i>co</i>-glycolic Acid)/Poly(styrene-<i>alt</i>-maleic Anhydride) Core/Shell Microparticles for Intrahepatic Arterial Chemotherapy of Hepatic Cancer

Davaa, Enkhzaya,Lee, Junghan,Jenjob, Ratchapol,Yang, Su-Geun American Chemical Society 2017 ACS APPLIED MATERIALS & INTERFACES Vol.9 No.1

<P>In this study, we demonstrated, that the MT1-MMP-responsive peptide (sequence: GPLPLRSWGLK) and doxorubicin-conjugated poly(lactic-co-glycolic acid/poly-(styrene-alt-maleic anhydride) core/shell microparticles (PLGA/pSMA. MPs) can be applied for intrahepatic arterial injection for hepatocellular carcinoma (HCC). PLGA/pSMA MPs were prepared with a, capillary-focused microfluidic device: The particle size, observed by scanning electron microscopy (SEM), was, around 22 +/- 3 mu m. MT1-MMP-responsive peptide and doxorubicin (D-OX) were chemically conjugated with pSMA segments on the shell of MPs to form. a PLGA/pSMA-peptide-DOX complex, resulting in high encapsulation efficiency, (91.1%) and loading content (2.9%). DOX was released from PLGA/pSMA-peptide-DOX MPs in a pH-dependent manner (similar to 25% at pH 5.4 and similar to 8% at pH 7.4) and accumulated significantly in an MT1-MMP-overexpressing Hep3B cell line. An in vivo intrahepatic injection study showed localization of MPs on the hepatic vessels and hepatic lobes up to 24 h after the injection without any shunting to the lung. Moreover, MPs efficiently inhibited tumor growth of Hep3B hepatic tumor xenografted mouse models. We expect that PLGA/pSMA-peptide-DOX MPs can be Utilized as an effective intrahepatic drug delivery system for the treatment of HCC.</P>

몽골 <며느리 고르기>형 민담 연구

( Davaa Uugantuya ) 숙명여자대학교 한국어문화연구소 2012 한국어와 문화 Vol.12 No.-

한국에서의 한국과 몽골 설화의 비교 연구는 1990년부터 다양한 면에서 활발하게 진행되고 있다. 설화 비교 연구는 각편 차원의 비교를 비롯하여 광포적인분포를 보이는 유형 설화 비교 및 불전소개 설화의 비교 등 크케 세 가지 흐름으로 진행되는 가운데 연구의 방향이 자료의 존재양상과 변이의 향방을 중시하는 실증적 논의에서부터 문화사적 관심, 여성의식 규명, 여성상징 등으로 다변화되고 있다. 본고는 수수께끼형 민담의 한 계통이라 할 수 있는 몽골의 <지혜로운 며느리이야기>설화를 <며느리 고르기>관점에서 구조의미를 살펴보고자 한다. 이는 한국의 그것과 비교 고찰, 더 나아가 한국과 몽골 구비문학 여성상 비교를 위한 예비적인 작업이라 할 수 있다. <지혜로운 며느리 이야기>설화는 몽골 유목민 생활속 여성의 삶과 며느리 고르기에 대한 의식을 구체적으로 반영하는 자료이기 때문이다. 지혜로운 여성에 대한 이야기는 세계적으로 퍼져있는 여성지혜담이며, 현명한처, 지혜로운 며느리 이야기로 나누어 보기도 한다. 본고는 몽골의 <지혜로운 며느리 이야기>를 <며느리 고르기>관점에서 살펴보았다. 몽골 <며느리 고르기>형 민담은 지혜로운 여인, 여인의 노부, 왕과 왕의 어리석은 아들에 얽힌 이야기다. 지혜로운 며느리는 그 자체로 중요한 모티프이며 흥미의 대상이다. 몽골 <며느리 고르기>형 민담은 온갖 수수께끼로 이루어져 있으며 그 해결자로 서민 여성이 등장하는 내용으로 주체가 여성이며, 여성의 지혜로 이야기가 구성되어 있다. 주어진 상황아래 여성인물은 내재된 능력에 의거하며 주체적인 방식으로 현실적 여건에 대응해 가는데, 내재된 주체적 능력의 발현은 지혜로 나타난다. 수수께끼형 과제문제의 답은 구체적이고 풍부한 경험을 통해 획득한 삶의 지혜의 소산이다. 또는 물음에 대한 언어적 답변이 아니라 일상 속에서 목축, 자연, 사물에 관계성에 대한 통찰의 힘으로 풀어낸 것이라 볼 수 있다. 본 민담에는 ``여성은 남성의 종이며, 집안의 비(婢)``라 하여 게르의 상석에도 앉을 권한이 없는 오직 남성에게 종속된 수동적 존재로 보았던 봉건주의 정신을 저항하려는 민중의 의식이 반영되어 있다. 여성의 지혜로 현실대응방식에서 발군의 능력을 보여 주어, 남편과 부왕에게 닥친 문제를 해결하여, 결국 가정의 주도권이나 국사를 일임하게 될 뿐만 아니라 왕위에 올라가게 되는 설정은 압제자 계급의 속성을 폭로하며, 저항하려는 민중들의 혁명적인 바람과 평민의 신분 상승 요구를 보여주고 있다고 말할 수 있겠으나 위기를 극복하고 난제를 해결하며, 미래를 개척함으로써 자신의 삶을 주도해나갔던 전통사회 속의 주체적 여성의 모습을 역설하고 있는 것으로 생각된다. The comparative studies of Korean and Mongolian folktales in Korea are being centered on three main streams. For instance individual folktale comparison, typological comparison and comparison of Buddhist scriptures. These studies are also diversified cultural meanings, feminine consciousness and symbolism as well as empirical study putting emphasis on the existent curcumstance and the changes of materials. This paper examines the meaning structure of Mongolian riddle-type folktale ``a wise daughter-in-law`` from ``picking up daughter-in-law`` viewpoint. It would be a comparison with Korean folktale and even the preliminary work of the comparative study on the image of women in Korean and Mongolian oral literature, because ``A wise daughter-in-law`` reflects the women``s life and the consciousness of picking up daughter-in-law custom in Mongolian nomadism. Folktales of wise women are around the world and deal with wise wife and wise daughter-in-law. This paper examines Mongolian folktale ``a wise daughter-in-law`` from ``picking up daughter-in-law`` viewpoint. Mongolian folktale ``a wise daughter-in-law`` is the story of wise woman and her old father, the king and his stupid son. Wise daughter-in-law is an important motif and a subject of interest in itself. In this story, common woman appear as main character and solve many riddles of the story with her wisdom. Under the given circumstances, she copes with the situation by her own inherent ability. Her inherent ability appears as wisdom. The answers to riddle-type problems are the product of wisdom of life gained through a lot of concrete experience. And they are not in verbal answer, but solved by the insight into the relationship among the nature and the things in ordinary farming. It reflects the public consciousness resisting the feudalism that regards Women as the subordinate to Men, saying ``Women are men``s servants and the household slaves, and so disqualified to sit at the head table of Ger(Mongolian traditional house)``. In this folktale, she solves the problems faced by her husband and his father, the king and takes the lead in home and responsibility for the national affairs, finally ascend to the throne by showing outstanding ability to cope with the reality with women``s wisdom. It discloses the attribute of ruler and shows the hope for the revolution and the status elevation of public. Also it emphasizes paradoxically express the independent women in traditional society that overcame a crisis, solved the difficulty, and paved the way for future.

Complexation of Adiponectin-encoding Plasmid DNA with Rosiglitazone-Loaded cationic Liposomes

( Enkhzaya Davaa ),( Ui Hyeon Jeong ),( Baek Ki Shin ),( Soon Gil Choi ),( Chang Seon Myung ),( Jeong Sook Park ) 한국약제학회 2010 Journal of Pharmaceutical Investigation Vol.40 No.6

To enhance therapeutic effects of insulin-sensitizing adipokine, ADN gene and potent agonists, rosiglitazone for the PPARγ, cationic liposomes as non-viral vectors were formulated. The particle size and zeta potential of drug loaded and unloaded cationic liposomes were investigated. The complex formation between cationic liposomes and negatively charged plasmid DNA was confirmed and the protection from DNase was observed. In vitro transfection was investigated in HepG2, HeLa, and HEK293 cells by mRNA expression of ADN. Encapsulation efficacy of rosiglitazone-loaded liposomes was determined by UV detection. Particle sizes of cationic liposomes were in the range of 110-170 nm and those of rosiglitazone-loaded cationic liposomes were in the range of 130-180 nm, respectively. Gel retardation of complexes indicated that the complex was formed at weight ratios of cationic lipid to plasmid DNA higher than 20:1. Both complexes protected plasmid DNA from DNase either drug free or drug loading. Encapsulation efficiency of rosiglitazone-loaded emulsion was increased by drug dose. The mRNA expression levels of ADN were dose-dependently increased in cells transfected with plasmid DNA. Therefore, cationic liposomes could be potential co-delivery system for drug and gene.

Photo-cured PMMA/PEI core/shell nanoparticles surface-modified with Gd-DTPA for T1 MR imaging

Enkhzaya DAVAA,Ha Neul LEE,Hyo Sang LIM,Chung Kil SONG,Don Haeng LEE,Sunintaboon PANYA,Su-Geun YANG 한국생물공학회 2014 한국생물공학회 학술대회 Vol.2014 No.4

Age determination of the Mongolian wild ass (Equus hemionus Pallas, 1775) by the dentition patterns and annual lines in the tooth cementum

Lkhagvasuren, Davaa,Ansorge, Hermann,Samiya, Ravchig,Schafberg, Renate,Stubbe, Anne,Stubbe, Michael The National Institute of Biological Resources 2013 Journal of species research Vol.2 No.1

Based on 440 skulls recently collected from two areas of the wild ass population in Mongolia, the time course of tooth eruption and replacement was investigated. The dentition pattern allows identification of age up to five years. We also conclude that annual lines in the tooth cementum can be used to determine the age in years for wild asses older than five years after longitudinal tooth sections were made with a low-speed precision saw. The first upper incisor proved to be most suitable for age determination, although the starting time of cement deposition is different between the labial and lingual sides of the tooth. The accurate age of the wild ass can be determined from the number of annual lines and the time before the first formation of the cementum at the respective side of the tooth.

Preparation of nanofibrous composite scaffolds for multiple delivery of drugs

Ganchimeg Davaa,Ahmed El-Fiqi,Cheol-Min Han,Hae-Hyoung Lee,Hae-Won Kim 대한치과재료학회 2014 대한치과재료학회지 Vol.41 No.S