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金光洙,李景道,安丙浚 충남대학교 약학대학 의약품개발연구소 1992 藥學論文集 Vol.8 No.-
Five isoflavones were isolated from the root of Caragana microphylla Lam. and the structures have been identified by means of chemical and physical methods. The isoflavones are 7-hydroxy-4'-methoxyisoflavone(formononetin), formononetin-7-O-β-glucoside(ononin), 7-hydroxy-3', 4'-methylenedioxyisoflavone(pseudobaptigenin), 7-O-β-glucuopyranosyloxy-3'-hydroxy-4'-methoxyi-soflavone(calycosin-7-O-β-gluc-oside) and maackiain.
( Dao Jing Xu ),( Ying Ze Zhao ),( Jin Wang ),( Juan Wen He ),( Ya Guang Weng ),( Jin Yong Luo ) 생화학분자생물학회 (구 한국생화학분자생물학회) 2012 BMB Reports Vol.45 No.4
Although previous studies have demonstrated that BMP9 is highly capable of inducing osteogenic differentiation of mesenchymal stem cells, the molecular mechanism involved remains to be fully elucidated. In this study, we showed that BMP9 simultaneously promotes the activation of Smad1/5/8, p38 and ERK1/2 in C3H10T1/2 cells. Knockdown of Smad4 with RNA interference reduced nuclear translocation of Smad1/5/8, and disrupted BMP9-induced osteogenic differentiation. BMP9-induced osteogenic differentiation was blocked by p38 inhibitor SB203580, whereas enhanced by ERK1/2 inhibitor PD98059. SB203580 decreased BMP9-activated Smads singling, and yet PD98059 stimulated Smads singling in C3H10T1/2 cells. The effects of inhibitor were reproduced with adenovirus expressing siRNA targeted p38 and ERK1/2, respectively. Taken together, our findings revealed that Smads, p38 and ERK1/2 are involved in BMP9-induced osteogenic differentiation. Also, it is noteworthy that p38 and ERK1/2 may play opposing regulatory roles in mediating BMP9-induced osteogenic differentiation of C3H10T1/2 cells. [BMB reports 2012; 45(4): 247-252]
김광수(Guang Zhu Jin),이경도(Jing Dao Li),안병준(Byung Zun Ahn) 대한약학회 1992 약학회지 Vol.36 No.5
Five isoflavones were isolated from the root of Caragana microphylla Lam. and the strictures have been identified by means of chemical and physical methods. The isoflavones are 7-hydroxy-4''-methoxyisoflavone(formononetin), formononetin-7-O-beta-glucoside(ononin), 7-hydroxy-3'', 4''-methylenedioxyisoflavone(pseudobaptigenin), 7-O-beta-glucuopyranosyloxy-3"-hydroxy-4"-methoxyisoflavone(calycosin-7-O-beta-glucoside) and maackiain.
Zhang, Yong-Guang,Liu, Qing,Wang, Hong-Fei,Zhang, Dao-Feng,Zhang, Yuan-Ming,Park, Dong-Jin,Kim, Chang-Jin,Li, Wen-Jun International Union of Microbiological Societies 2014 International journal of systematic and evolutiona Vol.64 No.6
<P>A facultatively alkaliphilic actinomycete strain, designated EGI 80088<SUP>T</SUP>, was isolated from a saline-alkali soil sample from Xinjiang province, north-west China, and subjected to a polyphasic taxonomic characterization. Strain EGI 80088<SUP>T</SUP> formed fragmented aerial hyphae and short spore chains, and rod-like spores aggregated at maturity. Whole-cell hydrolysates of the isolate contained <SMALL>ll</SMALL>-diaminopimelic acid as the diagnostic diamino acid, and glucosamine, mannose, galactose, glucose and rhamnose as the marker sugars. The major fatty acids identified (>5 %) were anteiso-C<SUB>15 : 0</SUB>, iso-C<SUB>15 : 0</SUB>, summed feature 4 (iso-C<SUB>17 : 1</SUB>I/anteiso-C<SUB>17 : 1</SUB>B), iso-C<SUB>16 : 0</SUB> and anteiso-C<SUB>17 : 0</SUB>. The predominant menaquinone was MK-9(H<SUB>4</SUB>). The G+C content of the genomic DNA of strain EGI 80088<SUP>T</SUP> was 70.6 mol%. EGI 80088<SUP>T</SUP> showed the highest 16S rRNA gene sequence similarity to its closest phylogenetic neighbour <I>Haloactinopolyspora alba</I> YIM 93246<SUP>T</SUP> (98.5 %). The DNA–DNA relatedness value of the strain EGI 80088<SUP>T</SUP> and <I>H. alba</I> YIM 93246<SUP>T</SUP> was 59.3±5.2 %. On the basis of morphological, chemotaxonomic and phylogenetic characteristics and DNA–DNA hybridization data, strain EGI 80088<SUP>T</SUP> represents a novel species of the genus <I>Haloactinopolyspora</I>, for which the name <I>Haloactinopolyspora alkaliphila</I> sp. nov. (type strain EGI 80088<SUP>T</SUP> = BCRC 16946<SUP>T</SUP> = JCM 19128<SUP>T</SUP>) is proposed. The description of the genus <I>Haloactinopolyspora</I> has also been emended.</P>
( Sheng Nan Li ),( Dao Sen Guo ),( Bo Guang Zhao ),( Jian Ling Ye ),( Jie Tian ),( Wen Qing Ren ),( Yun Wei Ju ),( Peng Cui ),( Rong Gui Li ) 한국미생물 · 생명공학회 2010 Journal of microbiology and biotechnology Vol.20 No.8
A cDNA encoding a cysteine protease inhibitor (CPI) was isolated from the cDNA library of clamworm Perinereis aibuhitensis Grube. The deduced amino acid sequence analysis showed that the protein had 51%, 48%, and 48% identity with Zgc: 153129 from Danio rerio, cystatin B from Theromyzon tessulatum, and the ChainA, stefin B tetramer from Homo sapiens, respectively. The gene was cloned into the intracellular expression vector pET-15b and expressed in Escherichia coli. The recombinant CPI (PA-CPI) was purified by affinity chromatography on Nicharged resin and ion-exchange chromatography on DEAE-Sepharose FF. The relative molecular mass of PACPI was 16 kDa as deduced by SDS-PAGE. Activity analysis showed that the recombinant protein could inhibit the proteolytic activity of papain. A constitutive and secretive expression vector was also constructed, and the cDNA encoding CPI was subcloned into the vector for extracellular expression. Western blotting analysis results showed that the PA-CPI was secreted into the medium. Bioassay demonstrated that E. coli DH5α harboring pUC18ompAcat-CPI showed a significant difference in mortality to the Asian longhorned beetle Anoplophora glabripennis compared with untransformed E. coli DH5α and control.
Y. C. Lin,Hui Yang,Dong‑Dong Chen,Dao‑Guang He 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.2
The intermediate-temperature (473–973 K) deformation behavior of a nickel-based superalloy is researched by uniaxialtensile experiments. It is observed that the flow characteristics and the Portevin–Le Chatelier (PLC) effects are significantlyaffected by the thermo-mechanical parameters. The serrated flow features are obvious under the testing conditions. A stackedauto-encoders (SAEs) network model is proposed for predicting the flow behaviors of the researched superalloy. The architectureof the established SAEs model is optimized layer by layer. The best number of hidden layer is 3, and the nodes perhidden layer are 15, 20 and 50, respectively. The excellent prediction ability suggests that the developed SAEs model canwell reconstruct the intermediate-temperature flow behavior involving the PLC effects of the researched superalloy.
Separation of arenols from a low-temperature coal tar by liquid-liquid extraction
Hua-Shuai Gao,Zhi-Min Zong,Zheng Yang,Dao-Guang Teng,Xiu-Hua Sun,Li Yan,Xian-Yong Wei,Qing-Jie Guo,Tian-Sheng Zhao,Hong-Cun Bai 한국화학공학회 2020 Korean Journal of Chemical Engineering Vol.37 No.5
Low-temperature coal tar (LTCT) is a sticky liquid mixture produced mainly from coal pyrolysis, which contains various value-added chemicals (VACs). Liquid-liquid extraction is considered as one of the green and effective ways to explore the organic composition and separate the VACs from LTCT. Herein, petroleum ether, methanol, and carbon disulfide were used to extract arenols from a LTCT. As a result, the relative content and absolute content of arenols extracted from the LTCT are ca. 96.3% and 85.9%, respectively. Among them, p-cresol is predominant, accounting for 22.2%. The isolated contents of arenols are up to 84.6%. Moreover, a kilogram-scale operation was carried out under the same conditions, which offers a potential application in industrial production.
Linh, Dinh Chi,Thanh, Tran Dang,Anh, Le Hai,Dao, Van Duong,Piao, Hong-Guang,Yu, Seong-Cho Elsevier 2017 JOURNAL OF ALLOYS AND COMPOUNDS Vol.725 No.-
<P><B>Abstract</B></P> <P>Influence of alkaline earth element doping in the A-site on the structure, magnetic and magnetocaloric properties of La<SUB>0.7</SUB>Ca<SUB>0.3-x</SUB>A<SUB>x</SUB>MnO<SUB>3</SUB> (A = Sr and Ba; <I>x</I> = 0, 0.15 and 0.3) perovskite manganites have been investigated. X-ray diffraction data indicates that samples <I>x</I> = 0 and 0.15 crystallize in the orthorhombic structure (space group <I>Pnma</I>), whereas samples <I>x</I> = 0.3 belong to the rhombohedral structure (space group <I>R</I>-3<I>c</I>). Substitution of Sr or Ba for Ca plays an important role in the increase of Curie temperature (<I>T</I> <SUB>C</SUB>) and modification the nature of the magnetic phase transition in materials. Sample <I>x</I> = 0 exhibits the first-order phase transition (FOPT) with a large magnetic entropy change (Δ<I>S</I> <SUB>m</SUB>), whereas samples <I>x</I> = 0.15 and 0.3 exhibit the second-order phase transition (SOPT) characterizations and the moderate values of Δ<I>S</I> <SUB>m</SUB>. Using the modified Arrott plots method, the critical behaviors around <I>T</I> <SUB>C</SUB> for SOPT samples have been investigated through the values of critical parameters (<I>T</I> <SUB>C</SUB>, <I>β</I>, <I>γ</I>, and <I>δ</I>). We pointed out that Ba-doping favors establishing long-range ferromagnetic order, contrary to the case of Sr-doping.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Sr or Ba-doped La<SUB>0.7</SUB>Ca<SUB>0.3</SUB>MnO<SUB>3</SUB> leads to modify the nature of the phase transition. </LI> <LI> Ba-doping favors establishing long-range FM order, contrast with Sr-doping. </LI> <LI> The effective exponents vary non-monotonically in the asymptotic region. </LI> </UL> </P>
Yan-Ling Chen,Xian-Guo Guo,Wen-Yu Song,Tian-Guang Ren,Lei Zhang,Rong Fan,Cheng-Fu Zhao,Zhi-Wei Zhang,Wenge Dong,Xiao-Bin Huang,Dao-chao Jin 대한기생충학ㆍ열대의학회 2023 The Korean Journal of Parasitology Vol.61 No.3
Chigger mites are the vector of scrub typhus. This study estimates the infestation status and ecological characteristics of chiggers on the chestnut white-bellied rat Niviventer fulvescens in Southwest China between 2001 and 2019. Chiggers were identified under the microscope, and infestation indices were calculated. The Preston’s log-normal model was used to fit the curve of species abundance distribution. A total of 6,557 chiggers were collected in 136 of 342 N. fulvescens rats, showing high overall infestation indices (prevalence=39.8%, mean abundance=19.2, mean intensity=48.2) and high species diversity (S =100, H’=3.0). Leptotrombidium cangjiangense, Neotrombicula japonica, and Ascoschoengastia sifanga were the three dominant chigger species (constituent ratio=42.9%; 2,736/6,384) and exhibited an aggregated distribution among different rat individuals. We identified 100 chigger species, with 3 of them (Leptotrombidium scutellare, Leptotrombidium wenense, and Leptotrombidium deliense) as the main vectors of scrub typhus in China and nine species as potential vectors of this disease. Disease vector occurrence on N. fulvescens may increase the risk of spreading scrub typhus from rats to humans. Chigger infestation on N. fulvescens varied significantly in different environments. The species abundance distribution showed a log-normal distribution pattern. The estimated number of chigger species on N. fulvescens was 126 species.