The Phytotoxic Effects of Selenium–Mercury Interactions on Root Growth in Brassica rapa (LvLing)

Zhi-Wei Bian,Jian Chen,Hui Li,Dan-Dan Liu,Li-Fei Yang,Yue-Lin Zhu,Wen-Li Zhu,Wei Liu,Zheng-Zheng Ying 한국원예학회 2016 Horticulture, Environment, and Biotechnology Vol.57 No.3

Rapid industrial and agricultural development has dramatically increased the emission of selenium (Se) and mercury (Hg) into the environment. Combined soil pollution by Se and Hg poses a potential threat to crop production. However, no toxic effects of Hg–Se interactions on plants have been reported previously. In this study, we investigated the effects of Hg–Se interactions on biochemical and physiological indices in the roots of Brassica rapa (LvLing). Seedlings were treated hydroponically with solutions of mercury chloride (1 μM), sodium selenite (4 μM), or a combination of the two. Combined Hg+Se treatment significantly inhibited root growth, reduced root biomass, and enhanced reactive oxygen species (ROS) and malondialdehyde accumulation and led to a loss of plasma membrane integrity. The combined treatment increased glutathione peroxidase, glutathione S-transferase, and peroxidase activity, reduced superoxide dismutase activity, and had no effect on catalase activity. In addition, we detected increased glutathione concentrations in root tips and reduced ascorbic acid concentrations in the presence of Hg+Se relative to individual treatments with these elements. Thus, Hg–Se interactions enhanced oxidative injury, cell death, and phytotoxicity in B. rapa roots.

Molecular cloning and expression analysis of a myosin light chain 1 (MLC‐1) gene from Indian meal moth Plodia interpunctella (Lepidoptera: Pyralidae)

Qiu‐Ning Liu,Dan-Dan Bian,Chun-Lin Zhou,Bo-Ping Tang 한국곤충학회 2015 Entomological Research Vol.45 No.6

Myosin light chain 1 (MLC‐1) protein acts in the organization, dynamics and transport processes associated with the cytoskeleton. In this work, an MLC‐1 gene was cloned and characterized from the Indian meal moth, Plodia interpunctella (Lepidoptera: Pyralidae). The isolated PiMLC‐1 cDNA is 913 bp, including a 5′‐untranslated region (UTR) of 79 bp, 3′‐UTR of 381 bp and an open reading frame (ORF) of 453 bp encoding a polypeptide of 150 amino acids, which contains two calcium binding domains (EF‐hands). The deduced PiMLC‐1 protein sequence has 39–94% comparison with other individuals. The qPCR analysis revealed that PiMLC‐1 was expressed in the four developmental stages (egg, larva, pupa and adult) and in all tissues tested, suggesting that it plays an important role in development of P. interpunctella. Based on the MLC‐1 amino acids, phylogenetic analysis showed a similar topology with the traditional classification, suggesting the potential value of the MLC‐1 protein in phylogenetic inference.

폴리메틸렌 다리로 연결된 양이온 이핵 CGC를 이용한 에틸렌과 1-헥센의 공중합

비안팽링(Feng Ling Bian),당황단웨이(Dang Hoang Dan Que),류원석(Won Seok Lyoo),이동호(Dong Ho Lee),노석균(Seok Kyun Noh),김용만(Yong Man Kim) 한국고분자학회 2007 폴리머 Vol.31 No.6

Cooking, Textural, Sensorial, and Antioxidant Properties of Common and Tartary Buckwheat Noodles

Yu Jie Ma,Xu Dan Guo,Hang Liu,Bian Na Xu,Min Wang 한국식품과학회 2013 Food Science and Biotechnology Vol.22 No.1

Noodles made from flour of 3 common and 2tartary buckwheat cultivars were investigated into cooking,textural, sensorial, antioxidant properties, and polyphenol composition. Common buckwheat noodles possessed greater tensility, lower adhesiveness, and better sensory properties. Buckwheat noodles were found to be good sources of dietary polyphenols and tartary buckwheat noodles contained a higher level of total phenolics (182.65-221.27 mg GA eq./100 g d.w.) and flavonoids (183.33-218.69 mg rutin eq./100 g d.w.) than their counterparts (p<0.05). Tartary buckwheat noodles also showed higher antioxidant capacity measured by reducing power, DPPH radical scavenging,and ABTS•+ scavenging assay. HPLC analysis identified and quantified 12 polyphenol compounds and revealed that rutin and quercetin were the dominant phenolic compounds. Among them, p-hydroxybenzoic acid, protocatechuic acid,rutin, and quercetin were higher in tartary buckwheat noodles, while common buckwheat noodles had greater contents of chlorogenic acid. Overall, buckwheat noodles are good alternative food with high nutritional quality.

Reliability Assessment of Distribution Network Considering Mobile Energy Storage Vehicles and Dynamic Zonal Coupling

Yang Wei,Wang Dan,Guo Jingming,Zhang Yijiao,Zhang Jiajia,Bian Zhaoyuan 대한전기학회 2024 Journal of Electrical Engineering & Technology Vol.19 No.1

Mobile energy storage spatially and temporally transports electric energy and has fexible dispatching, and it has the potential to improve the reliability of distribution networks. In this paper, we studied the reliability assessment of the distribution network with power exchange from mobile energy storage units, considering the coupling diferences among diferent time and zones of the vehicle–road–network system. First, we constructed a basic mobile energy storage operation framework that supports the distribution network reliability. Next, we proposed a quantitative model to describe the dynamic zonal coupled vehicle–road–network system. Based on this model, the corresponding charging and discharging scheduling strategies for diferent mobile energy storage units are well designed to improve the efciency of power support. Then, we provided a distribution network reliability assessment algorithm handling the power exchange from mobile energy storage units. Finally, we used the IEEE 33-node example system to clarify the necessity of the vehicle–road–network model in accounting for dynamic zonal coupling. We also analyzed the impact of diferent characteristics of mobile energy storage on the reliability of the distribution network, and verifed that one can improve the distribution network reliability by increasing the response speed, power capacity, or charging/discharging power of mobile energy storage vehicles.

Azohydromonas aeria sp. nov., isolated from air

Han Xue,Chun-gen Piao,Dan-ran Bian,Min-wei Guo,Yong Li 한국미생물학회 2020 The journal of microbiology Vol.58 No.7

A grey pink colored bacterium, strain t3-1-3T, was isolated from the air at the foot of the Xiangshan Mountain in Beijing, China. The cells are aerobic, Gram-stain-negative, non-sporeforming, motile and coccoid-rod shaped (0.9–1.2 × 1.9–2.1 μm). Strain t3-1-3T was catalase-positive and oxidase-negative and this strain grew at 4–42°C (optimum 28°C), a pH of 4.0–9.0 (optimum pH 7.0) and under 0–2% (w/v) NaCl (optimum 0–1% NaCl). A phylogenetic analysis based on 16S rRNA gene sequences revealed that strain t3-1-3T was closely related to Azohydromonas riparia UCM-11T (97.4% similarity), followed by Azohydromonas australica G1-2T (96.8%) and Azohydromonas ureilytica UCM-80T (96.7%). The genome of strain t3-1-3T contains 6,895 predicted protein-encoding genes, 8 rRNA genes, 62 tRNA genes and one sRNA gene, as well as five potential biosynthetic gene clusters, including clusters of genes coding for non-ribosomal peptide synthetase (NRPS), bacteriocin and arylpolyene and two clusters of genes for terpene. The predominant cellular fatty acids (> 10.0% of the total) in strain t3-1-3T were summed feature 3 (C16:1ω7c and/or C16:1ω6c, 37.8%), summed feature 8 (C18:1ω7c and/or C18:1ω6c, 29.7%) and C16:0 (17.3%). Strain t3-1-3T contained ubiquinone-8 (Q-8) as the predominant respiratory quinone. The polar lipids of strain t3-1-3T comprised phosphatidyl ethanolamine (PE), phosphatidyl glycerol (PG), diphosphatidyl glycerol (DPG), an unidentified glycolipid (GL), an unidentified aminophospholipid (APL), two unidentified phospholipid (PL1-2) and five unidentified lipid (L1-5). The DNA G + C content of the type strain is 70.3%. The broader range of growth temperature, assimilation of malic acid and trisodium citrate, presence of C18:3ω6c and an unidentified glycolipid and absence of C12:0 2-OH and C16:0iso differentiate strain t3-1-3T from related species. Based on the taxonomic data presented in this study, we suggest that strain t3-1-3T represents a novel species within the genus Azohydromonas, for which the name Azohydromonas aeria sp. nov. is proposed. The type strain of Azohydromonas aeria is t3-1-3T (= CFCC 13393T = LMG 30135T).

The complete mitochondrial genome of fall armyworm Spodoptera frugiperda (Lepidoptera:Noctuidae)

Bo-Ping Tang,Qiu-Ning Liu,Xin-Yue Chai,Dan-Dan Bian,Bao-Ming Ge,Chun-Lin Zhou 한국유전학회 2016 Genes & Genomics Vol.38 No.2

The mitochondrial genome (mitogenome) can provide important information for understanding molecular evolution and phylogenetic analyses. The complete mitogenome of Spodoptera frugiperda (Lepidoptera:Noctuidae) was determined to be 15,365 bp in length and has the typical gene order found in Noctuidae mitogenomes, it includes 13 protein-coding genes (PCGs), two rRNA genes, 22 tRNA genes and a A?T-rich region. The nucleotide composition was biased toward A?T nucleotides (81.09 %) and the AT skew of this mitogenome was slightly positive (0.004). All PCGs were initiated by ATN codons, except for cytochrome c oxidase subunit 1 (cox1) gene which was initiated by CGA. Eight of the 13 PCGs have the incomplete termination codon, T or TA. All the tRNA genes displayed the typical clover-leaf structure of mitochondrial tRNAs, with the exception of trnS1 (AGN). The A?T-rich region was 328 bp in length and consisted of several features common to the Noctuidae insects. Phylogenetic analysis showed that the S. frugiperda was within the Noctuidae.