錦江流域 淡水魚의 Sparganum에 關한 調査硏究

辛大煥,羅榮彦,權斗星 충남대학교 의과대학 지역사회의학연구소 1987 충남의대잡지 Vol.14 No.1

Prevalence of the plerocercoid larva in the fresh-water fishes in Keum-river basin was surveyed from April to September, 1986. To observe whether this plerocercoid larva is really sparganum or not, and can be transfered to the paratenic host or not, a study was performed with the adequate experimental animal (domestic fowl and cat). It was collected the 1096 fresh-water fishes that was ranged 8.0^-16.0cm in length. The results obtained were as follows; 1. A number of ova of Diphyllobothrium spp, was confirmed under the microscopic study, 5 days after experimental infection through the mouth, and them sacrificed to the experimental animal (final host, cat). The adult worm of Diphylobothrium spp., 9.5cm in length, was obtained and that worm was located in the upper position of rectum, Therefore, this plerocercoid larva was confirmed as Sparganum. 2. Transference to the paratenic host (dowestic fowl) was not resulted. 3. Among the total number of 1096 fresh-water fishes, only the Z. platypus was infected with 107 worms corresonding to 9.8%. 4. Infection rate according to the length of Z. platypus was 66. 4% at 12-13. 9cm in length, and this percentage was the highest prevalent rate.

리막탄(Rifamycin)의 痢疾아메바에 對한 治療 효과에 관한 연구

辛大煥 충남대학교 의과대학 지역사회의학연구소 1986 충남의대잡지 Vol.13 No.1

Rimactan (Rifamycine) is one of the most effective drugs for the treatment of tuberculosis and also shows promise in the treatment of certain nonmycobacterial diseases. A total 20 cases of E. histolytica cyst excretion patients were treated with Rimactan 10mg per body weight divided two for 20 days. All cases (20) showed negative convertion of E. histoltica cyst in the feces 7 days after beginning drug administration. 14 days and 20 days after beginning drug administration 17 cases (85%) out of 20 cases showed negative convertion of E. histolytica cyst, and 16 cases(80%) out of 20 cases were cured clinically and parasitologically 27 days after beginning drug administration (7 days after stopping drug administration). The tolerance of this drug was good, and side effects were noted mild and transient.

고양이의 Toxoplasma 抗體價 測定 및 腸內 奇生蟲 感染에 關한 硏究

辛大煥,金明海,李英河,羅榮彦 충남대학교 의과대학 지역사회의학연구소 1988 충남의대잡지 Vol.15 No.2

In order to confirm the intestinal helminthes, protozoa and Toxoplasma antibody titers from 41 cats, author performed the autopsy, stool examination, and Sabin & Feldman dye Test. 1. Total infection rates were 68.3%, and double infection rates were 29.3%. From 41 cats, 8 species were identified to Isospora felis(2.4%), Toxocara cati(7.3%), Ancylostoma sp(2.4%), Taenia taeniaeformis(51.2%), Diphyllobothrium mansoni(19.5%), Hymenolepis nana(2.4), Clonorchis sinensis (7.3,%), and metagonmus yokogawai(4.9%). 2. Habored region of each parasites was mainly small intestine, especially duodenum, and only Clonorchis sinensis was harbored in liver and bile duct. 3. Egg occurrence rate was 33.3% (T. taeniaeforis), 66.7(C. sinensis), and 100%(D. mansoni, M. yokogawai, I. felis). 4. Sabin & Feldman dye test titer was positive reaction in 14 cases corresponding to 34.190, and 27 cases were considered as negative.

忠南 一部地域의 寄生蟲 感染率 調査

辛大渙 충남대학교 의과대학 지역사회의학연구소 1977 충남의대잡지 Vol.4 No.2

A survey of intestinal parasites by fecal examination and intradermal test with the antigens of Clonorchis and Paragcnimus carried out in several localities of Chung-chung. nam Do, from August to September, 1977. A total of 1632 fecal specimens and intradermal test of 2278 cases were examined. And the results are summarized as follows: 1. The prevalence rate of intestinal helminth revealed 78.7% (male 76.3, female 81.7) in the 1632 fecal specimens and the rate of Ascaris lumbricoides showed 50.5%, Trichocephalus trichiurus 56.0%, hookworm 0.3%, Clonorchis sinensis 1.1%, Enterobius vermicularis 5.0%, Hymenolepis nana 0.7%, Taenia spp. 0.4%, and 1 case of each Fasciolidae and Trichostrongylus orientaifs, respectively. 2. The prevalence rate of intestinal protozoa in Anmyeon Myeon Seosan Gun was 16.4%(male 15.6, female 18.2) among 1050 stool specimens. The rate of each species showed Entamoeba histolytica 3.2%, Entamoeba coli 9.5%, Endolimax nana 3.5%, Giardia lamblia 5.0% and lodamoeba bu¨tschlii 0.1%. 3. The positive reaction rate of Clonorchis were 137 cases (6.0%) out of 2278 (male 1147, female 1131) intradermal tests, and the rate of each locality showed 21.5% in Jewon Myeon Kumsan Gun, 7.2% in Kereong Myeon Gongju Gun, 3.2% in Daehogie Myeon Dangjin Gun and 3.6% in Anmyeon Myeon Seosan Gun. 4. The Paragonimus positive reaction rate was 2.8% among the same intradermal cases and the highest rate showed in Jewon Myeon Kumsan Gun (10.0%).

病院 外來 患者에 있어서의 Toxoplasma 抗體價 分布에 關한 調査 硏究

辛大煥,徐志澤,李英河,羅榮彦 충남대학교 의과대학 지역사회의학연구소 1988 충남의대잡지 Vol.15 No.2

The results of Sabin & Feldman dye test for the detection of Toxoplasma antibody titer among 454 hospital outpatients were summarized as follows: 1. Among 454 test sera, 101 cases were positive, the positive rate was 22.2%. 2. According to age group, dye test positive rates were 33.3% (0-9 age group in years), 32.8% (40-49 age group in years), 31.6%(over 60 age group in years), 28.6%(10-19 age group in years), 20.1%(20-29 age group in years), 20.0%(30-39 age group in years), and 18.3%(50-59 age group in years). 3. According medical field, dye test postive rates were high in neurology(40%), pedatrics(37.5%), dermatology (36.4%), and surgery(30.6%) 4. Among 194 obstric and gynecological patients, abortion, anomaly, and retroversioflexion had high Toxoplasma antibody titers, but inflammatory disease, cancer, pregnancy and infertility revealed as relatively same antibody titers.

유혈목이(Natrix tigrina)의 腸間膜에 寄生한 鉤頭蟲의 Cystacanth幼蟲에 對한 硏究

辛大煥,沈雲澤 충남대학교 의과대학 지역사회의학연구소 1984 충남의대잡지 Vol.11 No.1

The intensity, morphology and tegumental ultrastructure of cystacanth of Acanthocephala is described from the mesntery of snakes(Natrix tigrina) which caught at a certain locality in Chungchung Buk-Do. The results are briefly summarized as follows: 1. The intensity of cystacanth in the mesentery of N tigrina showed 1,039 worms from 30 snakes, and larger snakes were infested more worms. 2. Morphology of cystacanth:conic form, 3.1^mm in length, 0.6^mm in width, aspinose on metasoma, neck absent. Proboscis nearly cylindrical, 0.75mmin length, 17 to 18 longitudinal, regularily alternating row of 24 to 25 spines. Proboscis receptacle double-walled. It has morphological similarity with the genus Centrorhynchus. 3. The cross-sectioned preparations of the cystacanth reavealed several differences from the proboscis, anteriall and middle portion of metasoma. Tegument, musculature and fragments of testis showed in the pseudocoelom of metasoma. 4. Scanning electron micrograph of the cystacanth showed the basic feature of the worm on light microscopical observations, except the more clearly and detailed arrangement of spines on proboscis. 5. The ultrastructure of the tegument of cystacanth showed seveveral different zones in their construction. These are the outermost epicuticle, plasma membrane, striped zone, vesicle zone, felt zone and radial zone. The thicker epicuticle was well developed and the layers beneath the cuticle contained numerous microtubule-like structures, vesicles, fibrous strands as well as glycogen particles.

Sparganum症 1例

신대환 충남대학교 의과대학 지역사회의학연구소 1985 충남의대잡지 Vol.12 No.2

Aug. 1985, live moving folded milky colored band like single worm, Sparganum, was collected from the abdominal wall of 22 year old male. It was 5mm in width and 7cm in length. Species identification was not performed.

혈액 투석 환자에서 중심정맥 협착에 대한 스텐트 삽입술 : Wallstent Placement

임대승,노상필,이유선,정승현,김보영,이정우,강정아,김정희,이민수,정준용,최시완,정진옥,성인환,이강욱,신영태 충남대학교 의과대학 의학연구소 2002 충남의대잡지 Vol.29 No.1

Stenosis of central vein is a common complication arising after percutaneous subclavian vein catheter insertion performed for temporary vascular access in chronic renal failure patients undergoing hemodialysis. There are several treatment methods for the condition like percutaneous angioplasty(PTA), stent insertion, and surgery, but recent trend is toward PTA and stents. Among the patients diagnosed with chronic renal failure from March 1993 to May 2002 and undergoing hemodialysis through AV fistula, the 14 Patients in whom central vein stenosis arose were selected for the study. A total of 28 percutaneous interventions(5 PTA and 23 stent placement) were performed, and restenosis rate and the time taken till the restenosis in de novo lesions and instant lesions were compared. All 28 cases were operated successfully. The 14 cases that received both anigioplasty and stent placement initially. (de novo lesion : 14 cases), Among the 10 cases with de novo lesion that followed up more than 1 year, 3 cases are currently undergoing hemodialysis without restenosis, and the remaining 7 cases have recurred stenosis with the mean time to restenosis of 10.9 months. In the 7 cases in whom stenosis recurred, 11 interventions were done(instent lesion: 11 cases). 4 of these were using only ballon angioplasty with 100% restenosis rate and the mean time of 3 months until restenosis. The remaining 7 cases were using both balloon angioplasty and stent placement, also with 100% restenosis rate but with the mean time of 12 months until restenosis, which was later than the group receiving only balloon angioplasty. In treating the patients with central vein stenosis, stent placement seems to be more advantageous over PTA in terms of restenosis rate and the mean duration of patency. In the case of instent lesion, inserting the stent for the second time after stenosis recurred lengthened the duration of patency compared to performing balloon angioplasty alone.

Oxantel/Pyrantel 合劑 및 Mebendazole에 依한 腸內線蟲類 驅蟲效果에 對한 比較實驗

金鍾煥,辛大煥,林定圭 충남대학교 의과대학 지역사회의학연구소 1978 충남의대잡지 Vol.5 No.1

A total of 63 subjects with multiple intestinal nematode infection: T. trichiura, A. lumbricoides, E. vermicularis and T. orientalis were treated with Oxantel/Pyrantel suspension and Mebendazole. The administration of Oxantel/Pyrantel suspension was 15mg/kg body weight in a single dose and Mebendazole was 600mg, divided by 100mg, twice daily for three consecutive days regardless of the body weight or ages. The results of present trials are summarized as follows: 1. The egg negative conversion rates for Oxantel/Pyrantel suspension were 100% in 20 cases of Ascariasis and in 3 cases of T. orientalis infection, and 92.3% in 26 cases of Enterobiasis. The egg reduction rate of T. trichiura was 84.8%. 2. The Mebendazole treated group showed the egg negative conversion rates of 100% in 27 cases of Ascariasis and one case of Trichostrongyliasis, 88.5% in 26 cases of Enterobiasis and 19.4% is 32 cases of Trichuriasis. The egg reduction rate of T. trichiura was 89.4%. 3. With the Oxantel/Pyrantel treated group, adult worms were found T. trichiura 1449 (average 45.3), A. lumbricoides 81 (av. 4.1) and E. vermicularis 249 (av. 9.6) worms. In Mebendazole treated group demonstrated the number of adult worms: 406(av. 13.1) of T. trichiura, 79 (av. 2.9) of A. lumbricoides and 134 (av. 5.2) of E. vermicularis, respectively. 4. Side effects of both Oxantel/Pyrantel and Mebendazole were few and mild.

Interferon-gamma 투여에 의한 Toxoplasma 감염 마우스의 면역학적 변화

이영하,신대환,심희숙 충남대학교 의과대학 지역사회의학연구소 1993 충남의대잡지 Vol.20 No.1

This study was performed to evaluate differences of immune responses according to the injection period of recombinant mouse interferon-γ(IFN-γ) in acute murine toxoplasmosis. Each mouse was infected intraperitoneally with 100 cysts of Beverley strain of T. gondii, and injected with 5 × 10 ^4 units of IFN-γ every other day for a total of two injections. From the 1st week after infection, blastogenic responses of splenocytes to concanavalin A(Con. A, 2.5㎍/ml) and Toxoplasma antigen(30.0㎍/ml) were examined at 1 week interval for 4 weeks. Also serum IgG and IgM antibody titers were examined by enzyme-linked immunosorbent assay, and immunohistochemical study of brain tissues were done at the same time. Toxoplasma antigen-treated blastogenic responses were significantly increased in the IFN-γ injected groups as compared with Toxoplasma-infected group. However Con. A-treated blastogenic responses were increased in the mice that received two doses of IFN-γ on day 4 and 2 before infection, or day 2 and 0 before infection. The serum IgG and IgM antibody titers of IFN-γ injected groups were increased from 2 weeks after infection, and IgM antibody titers were increased at 2 and 3 weeks after infection as compared with non-infected group. No significant differences were revealed between IFN-γ injected groups and Toxoplasma-infected group. T gondii was found as tachyzoites at 2 weeks after infection, and cysts found in 4 weeks. However, no T. gondii was detected in IFN-γ injected groups in the same period. The results suggested that IFN-γ administration to T. gondii-infected mice improve the cell-mediated immune responses, especially when IFN-γ was injected just before T. gondii infection. But there was not significant differences serum antibody titers and immunohistochemical findings of brain tissues according to the injection period of IFN-γ.