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      • Induction of heme oxygenase-1 protects against podocyte apoptosis under diabetic conditions

        Lee, Sang Choel,Han, Seung Hyeok,Li, Jin Ji,Lee, Sun Ha,Jung, Dong-Sub,Kwak, Seung-Jae,Kim, Seung Hye,Kim, Dong Ki,Yoo, Tae-Hyun,Kim, Jin Hyun,Chang, Se-Ho,Han, Dae Suk,Kang, Shin-Wook International Society of Nephrology 2009 Kidney international Vol.76 No.8

        Heme oxygenase-1 (HO-1) is an anti-oxidant enzyme normally upregulated in response to oxidant injury. Here we determined the role of HO-1 in podocyte apoptosis in glomeruli of streptozotocin-treated rats and in immortalized mouse podocytes cultured in media containing normal or high glucose. HO-1 expression, its activity, the ratio of Bax/Bcl-2 protein, and active caspase-3 fragments were all significantly higher in isolated glomeruli of diabetic rats and in high glucose–treated podocytes. These increases were inhibited by zinc protoporphyrin treatment of the rats or by HO-1 siRNA treatment of the podocytes in culture. The number of apoptotic cells was also significantly increased in the glomeruli of diabetic rats and in high glucose–treated podocytes. Inhibition of HO-1 accentuated the increase in apoptotic cells both in vivo and in vitro. Our findings suggest that HO-1 expression protects against podocyte apoptosis under diabetic conditions.

      • SCOPUSKCI등재

        Protective Effect of Heme Oxygenase-1 on High Glucose-Induced Pancreatic β-Cell Injury

        Lee, Eun-Mi,Lee, Young-Eun,Lee, Esder,Ryu, Gyeong Ryul,Ko, Seung-Hyun,Moon, Sung-Dae,Song, Ki-Ho,Ahn, Yu-Bae Korean Diabetes Association 2011 Diabetes and Metabolism Journal Vol.35 No.5

        <P><B>Background</B></P><P>Glucose toxicity that is caused by chronic exposure to a high glucose concentration leads to islet dysfunction and induces apoptosis in pancreatic β-cells. Heme oxygenase-1 (HO-1) has been identified as an anti-apoptotic and cytoprotective gene. The purpose of this study is to investigate whether HO-1 up-regulation when using metalloprotophyrin (cobalt protoporphyrin, CoPP) could protect pancreatic β-cells from high glucose-induced apoptosis.</P><P><B>Methods</B></P><P>Reverse transcription-polymerase chain reaction was performed to analyze the CoPP-induced mRNA expression of HO-1. Cell viability of INS-1 cells cultured in the presence of CoPP was examined by acridine orange/propidium iodide staining. The generation of intracellular reactive oxygen species (ROS) was measured using flow cytometry. Glucose stimulated insulin secretion (GSIS) was determined following incubation with CoPP in different glucose concentrations.</P><P><B>Results</B></P><P>CoPP increased HO-1 mRNA expression in both a dose- and time-dependent manner. Overexpression of HO-1 inhibited caspase-3, and the number of dead cells in the presence of CoPP was significantly decreased when exposed to high glucose conditions (HG). CoPP also decreased the generation of intracellular ROS by 50% during 72 hours of culture with HG. However, decreased GSIS was not recovered even in the presence of CoPP.</P><P><B>Conclusion</B></P><P>Our data suggest that CoPP-induced HO-1 up-regulation results in protection from high glucose-induced apoptosis in INS-1 cells; however, glucose stimulated insulin secretion is not restored.</P>

      • KCI등재SCIESCOPUS

        Original Article : Hydrogel Incorporated with Chestnut Honey Accelerates Wound Healing and Promotes Early HO-1 Protein Expression in Diabetic (db/db) Mice

        ( Dae Seong Choi ),( Sok Ho Kim ),( Youn Mook Lim ),( Hui Jeong Gwon ),( Jong Seok Park ),( Young Chang Nho ),( Jung Kee Kwon ) 한국조직공학과 재생의학회 2012 조직공학과 재생의학 Vol.9 No.1

        Proper wound management is an increasingly important clinical challenge and is a large and growing unmet need. Pressure ulcers, hard-to-heal wounds, and problematic surgical incisions are emerging with increasing frequency. At present, the wound-healing industry is experiencing a paradigm shift toward innovative treatments that exploit nanotechnology, biomaterials, and biologics. Here we determined the effectiveness of a radiation-processed hydrogel patch for the delivery of chestnut honey (CH) for the promotion of cutaneous wound healing in diabetic mice. CH is a natural compound that has antioxidant and bactericidal effects. Two full-thickness wounds were made on the dorsal side of diabetic (db/db) mice and the wounds were covered with hydrogel-incorporated CH. Time course observations revealed that mice treated with CH hydrogel showed accelerated wound closure and formation of granulated tissue, enhanced Ki-67 expression and early upregulated HO-1 proteins in the wound region compared with water hydrogel or non-treated mice. Taken together, these findings indicate that CH hydrogel can promote wound healing in diabetics with early HO-1 protein expression.

      • SCIESCOPUSKCI등재
      • SCIESCOPUSKCI등재

        Antioxidant and hepatoprotective effects of Korean ginseng extract GS-KG9 in a D-galactosamine-induced liver damage animal model

        Yun Ho Jo,Hwan Lee,Myeong Hwan Oh,Gyeong Hee Lee,You Jin Lee,Ji Sun Lee,Min Jung Kim,Won Yong Kim,Jin Seong Kim,Dae Seok Yoo,Sang Won Cho,Seon Woo Cha,Mi Kyung Pyo 한국영양학회 2020 Nutrition Research and Practice Vol.14 No.4

        BACKGROUND/OBJECTIVES: This study was designed to investigate the improvement effect of white ginseng extract (GS-KG9) on D-galactosamine (Ga1N)-induced oxidative stress and liver injury. SUBJECTS/METHODS: Sixty Sprague-Dawley rats were divided into 6 groups. Rats were orally administrated with GS-KG9 (300, 500, or 700 mg/kg) or silymarin (25 mg/kg) for 2 weeks. The rats of the GS-KG9- and silymarin-treated groups and a control group were then intraperitoneally injected Ga1N at a concentration of 650 mg/kg for 4 days. To investigate the protective effect of GS-KG9 against GalN-induced liver injury, blood liver function indicators, anti-oxidative stress indicators, and histopathological features were analyzed. RESULTS: Serum biochemical analysis indicated that GS-KG9 ameliorated the elevation of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH) in GalN-treated rats. The hepatoprotective effects of GS-KG9 involved enhancing components of the hepatic antioxidant defense system, including glutathione, glutathione peroxidase (GPx), superoxide dismutase (SOD), and catalase (CAT). In addition, GS-KG9 treatment inhibited reactive oxygen species (ROS) production induced by GalN treatment in hepatocytes and significantly increased the expression levels of nuclear factor erythroid-2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) proteins, which are antioxidant proteins. In particular, by histological analyses bases on hematoxylin and eosin, Masson"s trichrome, α-smooth muscle actin, and transforming growth factor-β1 staining, we determined that the administration of 500 mg/kg GS-KG9 inhibited hepatic inflammation and fibrosis due to the excessive accumulation of collagen. CONCLUSIONS: These findings demonstrate that GS-KG9 improves GalN-induced liver inflammation, necrosis, and fibrosis by attenuating oxidative stress. Therefore, GS-KG9 may be considered a useful candidate in the development of a natural preventive agent against liver injury.

      • 골수강내 금속정 고정을 이용한 경골 분절골절의 치료

        오형호,김대진 동국대학교 경주대학 1996 東國論集 Vol.15 No.-

        최근의 연구들은 경골 분절 골절에 대한 이해와 치료 방법에 대해 많은 향상을 가져왔지만 여전히 경골 분절 골절의 치료는 많은 문제점을 내포하고 있고 종종 만족스럽지 못한 결과를 초래하고 있다. 이는 경골의 분절골절이 대개 고 에너지 손상에 의해 발생하고 주위의 연부 조직에 심각한 손상을 동반하는 경우가 많기 때문이다. 교합성 골수강내 금속정 고정이 경골 분절골절의 일반적 치료방법으로 자리잡고 있는데 이 방법은 장관골 간부 골절에 있어 가장 안정적인 고정을 할수 있고 그로 인해 조기 거동과 체중 부하를 허용함으로써 기능회복에 좋은 결과를 초래하기 때문이다. 저자들은 1991년 1월부터 1994년 4월까지 19례의 경골 분절골절에 대해 교합성 골수강내 금속정 고정을 이용해 치료하고 다음과 같은 결과를 얻었다. 1.19례 모두에서 부가적인 치료방법없이 골유함을 얻었다. 2. 9례에서 근위 골절부위는 각형성이 된 채로 고정되면서 골유함이 지연되었다. 이러한 경향은 근위 골절부가 슬관절에 근접할수록 증가하였다. 3. 골수강내 금속정을 이용한 경골 분절골절의 치료에 있어 근위골절부의 정확한 정복과 고정은 난제로 남아 있어 이를 해결할 수 있는 수술방법의 개발이 필요할것으로 사료된다. Locked Intramedullary Nailing for Tibial Segmental Fracture Department of Orthopaedic Surgery, College of Medicine, DongGuk University Hyung Ho Oh M.D., Dae Jin Kim M.D. Although studies in recent years have significantly improved the understanding and treatment of segmental fractures of tibia, the treatment of segmental fractures of the tibia poses many problems and often the results are unsatisfactory, presumably because of the serious damage to surrounding soft tissues that usually occurs by the high energy injury. Interlocking intramedullary nailing has become more popularized because it is one affords of the most stable fixation for shaft fractures of long bones, which enables early mobilization and weight bearing and provide good functional recovery. We experienced nineteen segmental tibial fractures from January 1991 to April 1994.They were treated by interlocking intramedullary nails. The results were as follows. 1. In all the 19 cases, union was achieved without any additional procedures 2. In 9 cases, proximal fracture sites were fixed and united in angulated positions and union was delayed, especially when the proximal fracture site was close to the knee joint. 3. On managing segmental fractures of tibia with intramedullary nailing, proximal fracture site seems to have more difficulties in accurate reduction and fixation, so operative technical innovation and development in the fixation device seems to be necessary.

      • KCI등재후보

        알레르기에 대한 미역줄나무의 물 추출물의 효과

        변종호,강영성,김성수,김대한,황대룡,신민교,송호준 대한본초학회 2003 大韓本草學會誌 Vol.18 No.2

        Objective : To evaluate effect of allergy by Tripterygiwn regelii (TR), the biochemical changes associated with allergy were measured in BALF. serum splenocyte and lung of mice. Methods : After induction of allergic inflammation asthma by treatment of ovalbumine, water extracts from stem and leaf (SL) or flower (FL) of TR were taken orally for five successive days on mice, respectively. Result : In the total cell of BALF. the SL-TR extract (lCKhwk, h.w)-treated group was significantly reduced compared to control level and the reduction of eosinophil showed significantly at concentraton of 360 and loo0 dks, b.w. On the other hand, substantial decrease in IgE antibody formation was significantly observed by administration with SL-TR extract, whereas IgGZa was not affected. IgCl antibody formation appeared to be decreased inserum from SL-TR treated mice(l000 mg/kg, b.w). The content of IL-4 exhibited physiological role in splenocyte and lung was significantly reduced in the examined group compared to that of control after treatment of SL-TR extract(l@X/kg, b.w). In the case of IL-5, however, it was significantly reduced by FL-TR extract. In addition, IL-5 content in splenocyte was significantly reduced at concentration of 360 and loo0 d k g . b.w. by SL-TR dose, respxtively, and that of lung was significantly reduced dose-dewndently(l20, 360 and 1000mp/kg, b.w). Cnnclusion : It could be conduded that TripteTygiwn regelii showed beneficial effect on the allergic asthma and especially, its leaf and stem is more effective than flower.

      • Characterization of Antihypertensive Angiotensin I-Converting Enzyme Inhibitor from Recombinant E. coli

        Kim, Jae-Ho,Jeong, Seung-Chan,Lee, Dae-Hyoung,Lee, Jong-Soo 배재대학교 자연과학연구소 2005 自然科學論文集 Vol.16 No.1

        안지오텐신(ACE) 저해제는 항고혈 효과를 갖고 있으므로 오랫동안 고혈압의 예방이나 치료에 이용되어 왔다. 본 연구는 재조합 대장균으로부터 새로운 ACE 저해제를 생산하고 정제하며 나아가 이들이 구조-기능 관P를 규명하기 위해 수행되었다. Saccharomyces cerevisiase의 ACE 저해 펩타이드 유전자를 함유하고 있는 재조합 pGEX-4T-3을 대장균 BL21(DE3)로 형질전환 시켰다. 재조합 pGEX-4T-3을 갖고 있는 대장균 BL21(DE3)로부터 생산된 Glutathione-s 전이효소 (GST) 융합 단백질을 얻어서 그중 ACE저해 펩타이드를 Sephadex G-25 컬럼 크로마토그래피로 정제하였다. 정제된 ACE 저해 펩타이드는 타이로신-아스파틱엑시드-그리신-글리신-발린-패닐알라린-아르기닌-발린-타이로신-트레오닌의 서열을 가진 새로운 decapeptide이었고 ACE에 대하여 경쟁적으로 저해하였다. The angiotensin I-converting enzyme (ACE) inhibitor has anti-hypertensive effects and has long been used as prevention or remedy of hypertension. This study were carried out to produce and purify a new ACE inhibitor from recombinant E. coli and further elucidate its structure-function relationship. Recombinant pGEX-4T-3 containing ACE inhibitory peptide gene of Saccharomyces cerevisiae was transformed into E. coli BL21(DE3). Glutathione-S transferase (GST) fusion protein from E. coli BL21(DE3) harboring the recombinant pGEX-4T-3 was obtained and the ACE inhibitory peptide was purified with Sephadex G-25 column chromatography. The purified ACE inhibitory peptide was a novel decapeptide with sequence Tyr-Asp-Gly-Gly-Val-Phe -Arg-Val-Tyr-Thr which shows very low similarity to the other ACE inhibitory peptide sequence. The purified ACE inhibitor competitively inhibited ACE.

      • KCI등재

        Antioxidant and hepatoprotective effects of Korean ginseng extract GS-KG9 in a D-galactosamine-induced liver damage animal model

        Jo Yun Ho,Lee Hwan,Oh Myeong Hwan,Lee Gyeong Hee,Lee You Jin,Lee Ji Sun,Kim Min Jung,Kim Won Yong,Kim Jin Seong,Yoo Dae Seok,Cho Sang Won,Cha Seon Woo,Pyo Mi Kyung 한국영양학회 2020 Nutrition Research and Practice Vol.14 No.4

        BACKGROUND/OBJECTIVES: This study was designed to investigate the improvement effect of white ginseng extract (GS-KG9) on D-galactosamine (Ga1N)-induced oxidative stress and liver injury. SUBJECTS/METHODS: Sixty Sprague-Dawley rats were divided into 6 groups. Rats were orally administrated with GS-KG9 (300, 500, or 700 mg/kg) or silymarin (25 mg/kg) for 2 weeks. The rats of the GS-KG9- and silymarin-treated groups and a control group were then intraperitoneally injected Ga1N at a concentration of 650 mg/kg for 4 days. To investigate the protective effect of GS-KG9 against GalN-induced liver injury, blood liver function indicators, anti-oxidative stress indicators, and histopathological features were analyzed. RESULTS: Serum biochemical analysis indicated that GS-KG9 ameliorated the elevation of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH) in GalN-treated rats. The hepatoprotective effects of GS-KG9 involved enhancing components of the hepatic antioxidant defense system, including glutathione, glutathione peroxidase (GPx), superoxide dismutase (SOD), and catalase (CAT). In addition, GS-KG9 treatment inhibited reactive oxygen species (ROS) production induced by GalN treatment in hepatocytes and significantly increased the expression levels of nuclear factor erythroid-2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) proteins, which are antioxidant proteins. In particular, by histological analyses bases on hematoxylin and eosin, Masson's trichrome, α-smooth muscle actin, and transforming growth factor-β1 staining, we determined that the administration of 500 mg/kg GS-KG9 inhibited hepatic inflammation and fibrosis due to the excessive accumulation of collagen. CONCLUSIONS: These findings demonstrate that GS-KG9 improves GalN-induced liver inflammation, necrosis, and fibrosis by attenuating oxidative stress. Therefore, GS-KG9 may be considered a useful candidate in the development of a natural preventive agent against liver injury.

      • KCI등재

        김대건 (안드레아) 신부 얼굴복원

        한승호(Seung-Ho Han),박대균(Dae-Kyoon Park),이우영(U-Young Lee),고기석(Ki-Seok Kohl),김희진(Hee-Jin Kim),허경석(Kyung-Seok Hu),남용석(Yong-Seok Nam) 대한체질인류학회 2001 대한체질인류학회지 Vol.14 No.3

        본 작업은 서울대교구 명동성당에서 가툴릭의대에 우리나라 최초의 신부인 김대건 신부의 얼굴복원작 업을 의뢰해 1999년 9월부터 약15개월에 걸쳐 북원작업을 한 것으로 1971년 가툴릭의대 해부학교실에서 조사한 김대건 신부 머리뼈의 직접제측치와 사진 자료를 창조하여 제작하였다 작업의 과정은 머리뼈 계측값이 가장 비슷한 머리뼈를 찾아 복제를 하고 계측치에 맞게 수정한 후, 해부학 실습용 시체에서 직접계측하거나 초음파를 이용하여 간접계측을 한 한국인 얼굴의 표준두께를 적용하여 영굴을 복원하였다. 아울러 한국인에서 조사 한 머리뼈와 얼굴 각 부위의 연관관계자료를 토대로 유토를 이용한 복원작업을 시행하였으며 최종 결과물은 청동을 소재로 흉상을 만들었다. 이와 같은 작업은 법의학분야에서 신원미확인 머리뼈가 발견되었을 때 얼굴복원을 통해 신원을 확인하는 법 의인류학적 연구의 하나로, 한국인의 자료를 토대로 작업한 최초의 영굴복원이며 앞으로 신원확인을 위해 얼굴 을 복원하는 작업에 도움이 될 것으로 생각한다.

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