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RISS 인기검색어
- 검색키워드
- 저자 : DUONG SONG THAI DUONG (검색결과 2 건)
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Real-time and Label Free Detection of Biochemical Reaction Using Liquid Crystals
DUONG SONG THAI DUONG Gachon University 2021 국내석사
In this thesis, we describe the research of nematic liquid crystals (LCs)-based sensing technology to monitor biochemical reactions by investigating the optical response of LCs. Biochemical reactions could be sensitively detected with a polarizing light microscope due to the birefringence as well as the long-range anchoring transition of LCs. Herein, we observed the orientational transition of LCs influenced by (i) the enzymatic reactions of Human arginase 1 and ʟ-Arginine, and (ii) the biomolecular interactions of MUC1 and its specific aptamer. In (i) system, stearic acid is doped with 5CB to form a pH-dependent LCs. ʟ-arginine were hydrolyzed in the catalysis of ARG1 leading to the increase in pH. Then, stearic acid is deprotonated and adsorbed at the interface and change the LCs orientation. The shift on the LC alignment could be identified by an optical signal by using a polarizing light microscope. In (ii) system, the alignment molecules and bioreceptor are co-immobilized on the glass surface. While the alignment molecules anchor nematic LCs, bioreceptor capture the target. The surface topography changes and leads to the disrupt of LCs anchoring. The change in the LCs orientational could be observed by using a crossed polarizers. We described the LC-based sensing systems as a real-time and sensitive technique for imaging biochemical reactions.
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DUONG SONG THAI DUONG 가천대학교 글로벌캠퍼스 일반대학원 2025 국내박사
This dissertation presents research work dealing with rapid diagnostic methods for the monitoring of biomolecules and environmental contaminants through the use of liquid crystal (LC)-based sensing technology. The inherent elasticity of LC enables the amplification of slight changes in surface anchoring at the interface between the LC sensor and the target biomolecule, which facilitates optical detection using polarized optical microscopy (POM). Chapter 1 introduces the concept of biosensors, with a particular focus on LC-based biosensors and the properties of LC materials. A biosensor is defined as an analytical instrument consisting of components including: receptor, transducer, and signal processor, which together provide increased sensitivity and reliability. LC-based sensors detect analytes through observable optical changes, making them an effective tool for examination environmental, early diagnosis due to their simplicity and cost-effectiveness. Liquid crystalline materials can exist in different phases, transitioning between ordered and disordered states, and are categorized into thermotropic and lyotropic types. Birefringence, a key property of LCs, enables the study of optical variations in response to molecular ordering. Chapter 2 presents an LC-based assay for quantification sialic acid (SA). The sensor machenism is based on a synthetic bimetallic Co/2Fe MOF mimicking an oxidase and the NANA aldolase. After functionalization on TEM gold grids, the free SA was converted to pyruvate and N-acetyl-D- mannosamine in an aqueous solution by the catalysis of NANA aldolase. The subsequent Co/2Fe MOF-mediated reaction converts pyruvate to acetyl phosphate and H2O2, resulting in a sharp change in pH. This pH change is optically detected using LC doped with stearic acid. Chapter 3 presents a sensor that utilizes the electrostatic interactions between Cu²⁺ ions and DOPG molecules to detect glyphosate. The effectiveness of the sensor was qualitatively and quantitatively validated, with applications for environmental monitoring and agriculture investigated. Chapter 4 presents a qualitative detection method for phosphatidylserine (PS) using a waveform surface modification in conjunction with an Annexin V amplification system. The presence of annexin V contributed to enhance the optical signal, which significantly influenced mobilization of LCs and correlated with the changes in surface roughness. The sensor effectively detects PS by recognizing shifts in LC alignment. In chapter 5, an aptasensor was introduced with the aim to monitor acetamipride (ACE) using aptamers. ACE-antibody binding induces surface topography changes, resulting on potential repositioning of the LCs and changes in the optical signals. The observed optical intensities correlate with the ACE concentrations and allow a quantitative analysis of ACE in different samples.
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