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김두상(DS Kim),조재윤(JY Cho),김병렬(BR Kim),김종혜(JH Kim) 대한산부인과학회 1972 Obstetrics & Gynecology Science Vol.15 No.12
24세 미혼여성에 발생된 자궁근종의 1례를 보고하는 바이다. A case of nearly double fist sized submucous and intramural leiomyoma in a 24 year old unmarried woman was reported and a brief review of literature on etioloty and abnormal uterine bleeding was made.
난소암 환자에 대한 복합 화학요법 ( PAC Regimen ) 의 독성에 관한 연구
김두상(DS Kim),김문신(MS Kim),김용철(YC Kim),박형무(HM Park),유명숙(MS Yoo),신호문(HM Shin),김동명(DM Kim),조수현(SH Cho),조태승(TS Cho),환윤영(YY Hwang),심재철(JC Sim) 대한산부인과학회 1984 Obstetrics & Gynecology Science Vol.27 No.2
한양의대 산부인과학교실에서 1978년 10월부터 1982년 9월까지 PAC regimen(제1일에 adriamycin 50mg/㎡, 제2일에 cis-platinum 50mg/㎡, 제3~7일에 cyclophosphamide 150mg/ ㎡)으 로 화학치료를 받은 상피성난소암환자 16예를 대상으로 그 독성을 조사 분석하여 타 보고 자들의 성적과 비교 관찰한 바 다음과 같은 결론을 얻었다. 1. 혈액학적 독성은 총 16예중 10예(62.5%)에서 발생하였으며 혈소판 감소증은 3예 (18.8%), 백 혈구감소증 6예(37.5%), 빈혈 6예(37.5%)로 나타났으며 수혈은 1예(6.3%)에 실시하였다. 2. 신장에 대한 독성은 총 16예중 7예(43.8%)에서 각각 BUN증가 4예(25%) 혈청 creatinine 증 가 3예(18.8%)로 나타났으나, 전예가 경증이었다. 3. 가장 빈발하고 고통스러운 독성은 오심 구토로 전예에서 발생하였으며 perphenazine과 diazepam으로 어느정도 극복할 수 있었다. 4. 기타 말초신경독성은 5예(31.3%), 청력장애 1예(25%), 주관적인 시력장애 3예(18/8%), 간장 독성 1예(6.3%)였고 탈모증은 전예(100%)에서 발생하였다. The toxicity of PAC regimen was evaluated in 17 patients of ovarian epithelial carcinoma at the Edpartmane of Obstet. Nad Gynecol., Hanyang University Hospiatal from October, 1978 through september 1982. The PAC regimen consists of adriamycin 50mg/㎡ on the first day, cis-platinum 50mg/㎡ on the second day, and cyclophos-phanide 150mg/㎡ on the third to seventh day. It was repeated every 4 weeks. The results were as follow: 1. The hematologic toxicity occurred in 62.5%(10/16) of the patients, including thrombocytenia 18.8%(3/16), leukipenia 37.5%(6/16), and anemia 37.5%(6/16). For only one case, transfusion was require. 2. Nephrotoxicity occurred in 43.8%(7/160), including an elevation of BUN 25.0%(4/16) and an elevation of serum creatinine 18.8%(3/16). But all were in mild degree. 3. For the symptoms of gastrointestinal tract were nausea and vomiting which occurred in all cases and most distressing problems. All patients, however, were well tolerated with perphenazine and diazepam. 4. Peripheral neurotoxicity occurred in 31.3%(5/16), ototoxicity in 25.0%(1/4), blurred vision in 18.8%(3/16), hepatotoxicity in 6.3%(1/16) and alopecia in 100%.
조기양막파수에 의한 양수내감염증 및 신생아 이환율을 진단하는데 있어서 CRP 및 LDH의 역할
김수평,이종건,김진홍,노덕영,박대순,김인재,이선희,나욱열 대한산부인과학회 1990 Obstetrics & Gynecology Science Vol.33 No.5
조기양막파수의 원인과 결과로서 양수내감염증은 논란의 여지가 있으며, 양수내감염증에 이차적으로 태아는 자궁내감염기회가 생길 수 있다. 양수내감염과 신생아 이환율을 진단하는데 있어서 신뢰도를 평가하기 위하여 조기파수된 산모와 이들 산모로부터 태어난 태아로부터 혈중 CRP와 혈중 및 양수의 LDH를 측정하여 다음과 같은 결론을 얻었다. 1. 조기양막파수 산모에서 양수내감염증은 CRP양성인 경우를 근거로 했을때 CRP양성인 경우가 8예로서 양수감염율은 28.6%였다. 2. 조기양막파수 산모에서 혈청 LDH값은 CRP양성군과 음성군간에 유의한 차이가 없었으나 400IU/L를 기준으로 한 양수내 LDH값과 양수내 LDH와 혈청 LDH비 1.5를 기준으로 구분한 경우 양 군간에 유의한 차이가 있었다.(P$lt;0.05) 3. 양막조기파수 산모에서 태어난 신생아의 체중은 2500gm이상인 경우가 CRP음성군에서 83.3%, CRP양성군에서 16.7%로 유의한 차이를 보였다. 4. 파막후 24시간이 지난 산모 22명으로부터 태어난 신생아에서 CRT양성율은 27.2%였으며,CRP양서인 신생아에서 폐염, B형 간염, 급성위장관염, 신생아고빌리루빈혈증 및 미숙아의 이환율을 보였다. Amniotic fluid infection as the cause and the consequence of premature rupture of membrane is controvertible. Following the amniotic fluid infection, the fetus was susceptible to the intrauterine infection. In order to evaluate their reliability as a diagnostic tool of amniotic fluid infection and neonatal morbidity, LDH in serum, amniotic fluid and CRP of pregnant women with premature rupture of membrane and neonates delivered from them were measured. The results were as follows: 1. CRP, microbiologic findings and amniotic fluid infection rate: Considering positive CRP as amniotic fluid infection, amniotic fluid infection rate was 28.6 % (8/28) Microorganisms were isolated from all of the amniotic fluid specimens of CRP(+) eight cases by bacterial culture, but above 20-30 WBC/ml was revealed by Gram stain in seven cases of the CRP (+) eight cases. 2. CRP, LDH and histopathological findings of placentas: There was no significant difference in the level of serum LDH between CRP (+) and CRP (-) group. LDH greater than 400 IU/L and the ratio of amniotic fluid LDH/ serum LDH greater than 1.5, have a high correlation with amniotic fluid infection. There was no correlation between histologic reports of chorioamniotis and amniotic fluid infection. Based on more than 100 WBC/ml in amniotic fluid, there was no correlation between the number of WBC and amniotic fluid infection. 3. Neonatal morbidity and mortality. There was no difference in gestational age, Apgar score at 5 min, RDS, neonatal sepsis between maternal CRP (+) group and CRP (-) group, but significant difference was found in the neonatal birth weight. 4. Neonatal morbidity from prolonged rupture of membrane: The incidence of CRP (+) in the infants delivered from mother with prolonged rupture of membrane are 27.2 % (6/22). The CRP (+) infants have neonatal morbidity such as pneumonia, hepatitis B, acute gastroenteritis, neonatal hyperbilirubinemia and prematurity. In conclusion, these results suggest that not only the measurements of CRP from the pregnant women with PROM and their neonate but also the measurements of LDH in the amniotic fluid and the rate of amniotic fluid LDH/serum LDH might be useful for a screening test of amniotic fluid infection and neonatal morbidity.
난소과립막세포 스테로이드호르몬 생성 및 증식에 대한 Mullerian Inhibiting substance의 효과
김장흡,이진우,김수평,나종구,김은중,김태응,신재인,제동성 대한산부인과학회 1996 Obstetrics & Gynecology Science Vol.39 No.12
Ovarian follicular growth is a consequence of granulosa cell proliferation, and steroid production by these cells appears to be a major determinant of the endocrine microenvironment of ovum maturation. The gonadotropins, FSH ad LH, regulate directly the growth and differentiation of the granulosa cells in the ovary, but there is evidence to suggest that the gonadotropins act partly through locally produced growth factors and that this interaction is complex. A number of growth factors, such as epidermal growth factor(EGF), insulin-like growth factor-I(IGF-I), and transforming growth factor-β(TGF-β) are produced in the ovarian follicle and might act in an autocrine and/or paracrine manner to directly control granulosa cell proliferation and differentiation. While it has been demonstrated that steroid hormones, gonadotropins and growth factors regulate proliferation and differentiation of ovarian follicles, little is known concerning the fators involved in the inhibition of ovarian function. Recently, Mullerian inhibiting substance(MIS), a non-steroidal testicular Sertoli cell product responsible for the regression of Mullerian duct in male embryo, has been shown to be produced by ovarian granulosa cells in adolescnet and ault females. Although the function of MIS in the ovary has not been fullydelineated, MIS appears to be a regulator of oocyte maturation and follicular development in the rat. In this study, in order to investigate the influence of MIS on steroidogenesis and proliferation of human granulosa cells, we performed culture of human granulosa cells. The cells were cultured for 2 to 12 days under two conditions, with and without MIS(20 ng/ml). Each condition was additionally defined by the presence and absence of EGF(20 ng/ml), FSH(10 ng/ml), or LH (10 ng/ml). Estradiol, progesterone, and testosterone were mearsured form the spent media by radioimmunoassay and the cell number was determined by trypsinizing the cells and counting them with a Coulter counter. The result were as follows: 1. There was about 6-fold increase in the final granulosa cell number when the culture were maintained for 12 days in Ham`s F-10 supplemented with 10% MIS-free female fetal calf serum(control). FSH and EGF caused a significant increase in granulosa cell number compared with the control but LH significantly suppressed cell number after 8 days in culture. 2. MIS caused a significant decrease in granulosa cell number compared with the control after 8 days in culture in the 20 ng/ml dose, and on day 12 in the 2 ng/ml dose(p$lt;0.05). MIS(20ng/ml)also significantly blocked EGF induced proliferation of granulosa cells after 6days in culture and the maximum effect was observed on day 12 of culture(p$lt;0.01). 3. Estradiol production from granulosa cells was significantly stimulated by both gonadotropins, with LH having the greater effect than FSH after 6 days in culture(p$lt;0.05). However, EGF significantly suppressed estradiol production of granulosa cells, but significantly decreased FSH induced estradol production of granulosa cells after 4 days in culture(p$lt;0.05). 4. Progesterone production from granulosa cells was significantly stimulated by both gonadotropins and EGF, with LH having the greatest effect. However, MIS had no effect on basal progesterone production of granulosa cells after 4 days in culture(p$lt;0.05) with havint the greatest effect on day 10 of culture(p$lt;0.01). 5. Testosterone production from granulosa cells was not detected in all conditions. In conclusion, these experiments demonstrate that MIS may have relevant funciton in the ovay as a regulatior of follicular development and steroidogenesis maturation during the adult reprodu-ctive cycle.
Exogenous DNA Uptake of Boar Spermatozoa by a Magnetic Nanoparticle Vector System
Kim, TS,Lee, SH,Gang, GT,Lee, YS,Kim, SU,Koo, DB,Shin, MY,Park, CK,Lee, DS Blackwell Publishing Ltd 2010 REPRODUCTION IN DOMESTIC ANIMALS Vol.45 No.5
<P>Contents</P><P>The sperm-mediated gene transfer method is applicable to transgenesis in many species that use spermatozoa for reproduction recently, which has been shown various results. In the current study, we show that transgenic porcine embryos can be efficiently produced by employing a simple transfection method that uses magnetic nanoparticles (MNPs). The complexes formed between plasmid DNA and MNPs were bounded on ejaculated boar spermatozoa at a higher efficiency compared to methods using DNA alone or lipofection. Using confocal microscopy, rhodamine fluorophore-labelled MNPs were detected on external surfaces of the spermatozoa membrane, which were bounded on zona pellucida of <I>in vitro</I> maturated oocyte during <I>in vitro</I> fertilization. Electron microscopy revealed that clusters of MNPs were detected in inside of plasma membrane and nucleus of the spermatozoa head. Additionally, we found that magnetofected boar spermatozoa could be fertilized with oocytes <I>in vitro</I> and that the resulting gene of green fluorescent protein was detected in fertilized eggs by genomic PCR analysis. Taken together, these results suggest that MNPs can be used to efficiently introduce a transgene into embryo via spermatozoa.</P>