FK506이 T 림프구 사멸에서 활성산소 생성에 미치는 영향

이호균(Ho Kyun Lee),정상영(Sang Young Chung),최수진나(Soo Jin Na Choi) 대한외과학회 2009 Annals of Surgical Treatment and Research(ASRT) Vol.77 No.5

Purpose: Tacrolimus (FK506) has been widely used as an immunosuppressant in organ transplanted recipients to suppress organ rejection phenomenon. We investigated the role of oxidative stress and heme oxygense-1 by FK506 on human Jurkat T cells. Methods: The cells viability was examined by DAPI stain, enzyme activity of caspase family proteins, and western blotting for Baks, PUMA, iNOS, HO-1. Cells were cultured in the absence or presence of CoPPIX or ZnPPIX and the fluorescence intensity was analyzed using a flow cytometry. Results: Treatment with FK506 increased the generation of reactive oxygen species (ROS), including hydrogen peroxide and superoxide anion, and NO in Jurkat cells in a dose-dependent manner. Immunohistochemistry and Western blot analysis data revealed the hemoxygenase-1 (HO-1) was induced by the addition of FK506 in Jurkat cells. Induction of CoPP, HO-1 inducer, resulted in decreased intracellular H₂O₂ and NO concentrations. Instead ZnPP, an HO-1 competitive inhibitor did it reversely. In addition, ZnPP regulates iNOS protein synthesis by inhibition of HO-1. Conclusion: Increase of HO-1 expression would induce to decrease the intracellular H₂O₂ and NO concentrations. Also, HO-1 would regulate iNOS protein synthesis. Consequently, we can expect the regulation of HO-1 expression with concomitants use of FK506 to suppress organ rejection phenomenon by enhancing apoptosis.

한강의 Microflora 에 관한 연구 (제2보 ) - 춘천 및 청평저수지를 중심으로 한 한강의 식물성 plankton 과 그 계절적 소장

정영호(Yung Ho Chung),계응서(Eung Suh Kay),박덕환(Duck Whan Park) 한국식물학회 1968 Journal of Plant Biology Vol.11 No.2

The Han River was investigated monthly from April through Septemher, 1967 at Chunchon reservoir, Shin-yean, So-yang, Chung-pyong reservoir, Yang-soo-ri and Gi-doo-won to clarify the phytoplankton and to study its seasonal variation. At the same time, vertical distribution of phytoplankton was also studied from June through September at Chun-chon and Chung-pyong reservoir. The results obtained are as follows: As the environmental conditions, water temperature, dissolved oxygen, pH, and transparency were determined. The plankton samples collected from six stations were identified and classified by Engler`s classification system. It resulted in 4 phyla, 4 classes, 3 subclasses, 13 orders, 27 families, 5 tribes, 71 genera, 222 species, 1 subspecies, 51 varieties and 2 forms. The total number of Phytoplankton identified was 276. Of them, 84 species, 1 subspecies 26 varieties, 2 forms were recorded in this paper for the first time. Considering with the habitat of the total number of phytoplanktons, the highest percentage of Limnoplankton existed in the Chung-pyong reservoir. It suggests that the Chung-pyong reservoir is becoming to have the character of lake. The planktons appeared comnonly during six months at six stations belong to Chrysophyta including 22 species in 11 genera. Among these 22 species. Navicula, consists of 6 species, was the most abundant. The number of species identified uniquely at each station were 5 species in the Chun-chon reservoir, 6 in Chung-pyong and Yang-soo-ri, 8 at Shin-yean, 11 at So-yang, and 18 at Gi-doo-won. The number of species occurred at all stations were 29 in spring, 42 in Autumn and during investigation period. The maximum production of total phytoplankton was generally shown in the late spring and the early fall with differences in quantity at six collecting stations. In the Chun-chon reservoir, the high concentration of approximately 1,190,000 cells per litter in the months of August and September was due to the increase of Chrysophyte genus, Melosira and the highest concentration of approximately 2,000,000 in July at Gi-doo-won was caused Chlorophyte genus, Secenedesmus. The Chun-chon reservoir of three years old was different from the Chung-pyong reservoir of 25 years old with respect to increase in Melosira genus in August and September. After the completion of the Ui-am reservoir, though there was an increase in total density, the number of species of phytoplankton was not changed. It suggests that Ui-am reservoir is similar to the Chun-chon reservoir under the influence of the latter. The density of total phytoplankton was generally high at the depth of 2m to 5m for the two reservoirs.

Curcumin protects retinal pigment epithelial cells against oxidative stress via induction of heme oxygenase-1 expression and reduction of reactive oxygen

Woo, Je Moon,Shin, Da-Yong,Lee, Sung Ju,Joe, Yeonsoo,Zheng, Min,Yim, Jin Ho,Callaway, Zak,Chung, Hun Taeg Molecular Vision 2012 Molecular vision Vol.18 No.-

<P><B>Purpose</B></P><P>To determine whether curcumin induces expression of the defensive enzyme heme oxygenase-1 (HO-1) and protects cells against oxidative stress in cultured human retinal pigment epithelial cells.</P><P><B>Methods</B></P><P>Effective concentrations and toxicities of curcumin were determined after 3 h of curcumin treatment with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Confluent human retinal pigment epithelium cell lines (ARPE-19) were preincubated with curcumin and oxidatively challenged with H<SUB>2</SUB>O<SUB>2</SUB>. HO-1 expression was determined with western blot analysis. To confirm the protective role of HO-1 in oxidative stress, small interfering RNA (siRNA) against HO-1 or inhibitor of HO-1 was treated with curcumin in retinal pigment epithelium cells. Intracellular levels of reactive oxygen species (ROS) were measured with flow cytometry and confocal microscopy. Apoptosis was evaluated with Annexin V-fluoroscein isothiocyanate staining.</P><P><B>Results</B></P><P>Curcumin had little cytotoxicity at concentrations less than 30 μM, and HO-1 expression was the highest at the 15 μM concentration. At this concentration, curcumin also increased the cytoprotective effect against the oxidative stress of H<SUB>2</SUB>O<SUB>2</SUB> through the reduction of ROS levels in human retinal pigment epithelial cells. Curcumin’s effect on the reduction of ROS was mediated by the increase in HO-1 expression.</P><P><B>Conclusions</B></P><P>Curcumin upregulated the oxidative stress defense enzyme HO-1 and may protect human retinal pigment epithelial cells against oxidative stress by reducing ROS levels.</P>

연잎, 연자육, 연자방 에탄올 추출물의 항염증 활성

이은주(Eun-Joo Lee),서유미(Yu-Mi Seo),김용현(Yong-Hyun Kim),정정욱(Chungwook Chung),성화정(Hwa-Jung Sung),손호용(Ho-Yong Sohn),박종이(Jong-Yi Park),김종식(Jong-Sik Kim) 한국생명과학회 2019 생명과학회지 Vol.29 No.4

연은 아시아 국가에서 음식과 약재로 널리 사용되는 재료이다. 본 연구에서는 연의 잎(leaf, NL), 자육(seed, NS), 자방(seedpod, NSP)으로부터 에탄올 추출물을 제조하고 이들의 항염증 활성과 작용기전을 규명하였다. 이들의 항염증 활성을 연구하기 위하여 LPS로 자극된 RAW 264.7 세포에서 nitric oxide (NO) 생산을 측정하였다. NL, NS, NSP는 세포 생존율에 영향을 주지 않으면서, 농도의존적으로 NO의 생산을 현저하게 저해하였으며, iNOS 및 COX-2와 같은 pro-inflammatory 중재자들의 단백질 발현을 감소시켰다. 또한, NL, NS, NSP는 MAPKs 단백질의 인신화를 감소시키고 NF-κB p65의 핵으로의 이동을 저해함으로써, 세 추출물에 의한 항염증 활성은 MAPKs 경로와 NF-κB 경로를 조절함으로써 이루어짐을 제시한다. 게다가, ROS의 생성이 세 추출물에 의해서 모두 저해되었으며, HO-1의 발현과 HO-1의 전사조절인자인 Nrf2의 핵으로의 이동이 증가되었다. 결론적으로, 이러한 연구결과는 연의 다양한 부위의 추출물인 NL, NS 그리고 NSP는 항염증 활성을 가지고 있으며, MAPKs, NF-κB, Nrf2/HO-1 등 다양한 신호경로를 통해 조절할 수 있음을 제시한다. Nelumbo nucifera, also known as sacred lotus, has mainly been used as a food throughout the Asian countries. In the present study, we prepared ethanol extracts from leaf (NL), seed (NS), and seedpod (NSP) of Nelumbo nucifera and investigated their anti-inflammatory activities in mouse macrophage RAW 264.7 cells. To evaluate the anti-inflammatory activities of NL, NS, and NSP, nitric oxide (NO) production was measured in LPS-stimulated RAW 264.7 cells. NL, NS, and NSP significantly reduced NO production in a dose-dependent manner without affecting cell viabilities. NL, NS, and NSP dramatically decreased the protein expression of pro-inflammatory genes such as iNOS and COX-2. NL, NS, and NSP also suppressed phosphorylation of MAPKs and the nuclear translocation of NF-κB p65 indicating they have their anti-inflammatory activities via regulating mitogen-activated protein kinases (MAPKs) and nuclear factor kappa B (NF-κB) pathways. In addition, we analyzed the production of reactive oxygen species (ROS) by the treatment of NL, NS, and NSP. All extracts reduced ROS production in a dose-dependent manner. And also, they increased heme oxygenase-1 (HO-1) protein expression and the nuclear translocation of nuclear respiratory factor 2 (Nrf2). In conclusion, our results suggest that Nelumbo nucifera has its anti-inflammatory activity via regulating MAPKs, NF-κB, and Nrf2/HO-1 pathways.

건조 상추 에탄올 추출물의 항염증 활성

이은주(Eun-Joo Lee),서유미(Yu-Mi Seo),김용현(Yong-Hyun Kim),정정욱(Chungwook Chung),성화정(Hwa-Jung Sung),손호용(Ho-Yong Sohn),박종이(Jong-Yi Park),김종식(Jong-Sik Kim) 한국생명과학회 2019 생명과학회지 Vol.29 No.3

상추는 가장 선호하는 녹색 채소 중 하나이다. 상추는 폴리페놀성 화합물을 비롯한 다양한 성분을 함유하고 있으며, 항균, 항산화, 항염증 등의 생리활성을 가지고 있는 것으로 알려져 있다. 본 연구에서는 건조상추의 에탄올 추출물(DLE)을 제조하고 이들의 항염증 활성을 연구하였다. DLE의 항염증 활성을 측정하기 위하여 LPS로 활성화된 마우스 대식세포 RAW 264.7 세포주에서 nitric oxide (NO) 생성을 측정하였다. DLE는 세포주의 생존에는 영향을 미치지 않으면서 NO 생산을 현저하게 저해하였다. DLE에 의해 염증 유전자인 iNOS와 COX-2의 유전자와 단백질의 발현이 모두 감소하였으며, 6개의 염증관련 cytokine 유전자(IL-1α, IL-1β, IL-1F6, TNF-α, CSF2, 그리고 CXCL10)의 발현이 모두 감소하였다. 또한, DLE의 처리는 MAPKs 경로의 인산화를 모두 저해하였으며, NF-κB p65의 핵으로의 이동을 저해하였다. 이러한 결과는 DLE의 항염증 활성은 MAPKs 경로와 NF-κB 경로를 조절함으로써 이루어짐을 시사한다. 또한, DLE는 농도의존적으로 reactive oxygen species (ROS)의 생산을 저해하였으며, hemeoxygenase-1 (HO-1) 단백질의 발현을 증가시켰으며, HO-1의 전사조절인자인 Nrf2의 핵으로의 이동을 증가시켰다. 결론적으로, 이러한 연구결과는 DLE가 염증관련 유전자의 발현을 감소시키며, MAPKs, NF-κB, 그리고 Nrf2/HO-1 등 다양한 경로를 조절함으로써 항염증 활성을 가지는 것을 제시한다. Lettuce (Lactuca sativa L.) is one of the most popular green leafy vegetables, and it contains various beneficial components including polyphenolic compounds and has been known to possess various biological functions such as anti-microbial, anti-oxidative, and anti-inflammatory activities. In the present study, we prepared ethanol extract of dried lettuce (DLE) and investigated its anti-inflammatory activity. To evaluate the anti-inflammatory activity of DLE, nitric oxide (NO) production was measured in LPS-activated mouse macrophage RAW 264.7 cells. DLE significantly suppressed NO production in these cells without affecting cell viabilities while resveratrol was used as a positive control. DLE dramatically decreased the expression of pro-inflammatory genes such as iNOS and COX-2 at the mRNA and protein levels and reduced the expression of several cytokines including IL-1α, IL-1β, IL-1F6, TNF-α, CSF2 and CXCL10. In addition, DLE suppressed phosphorylation of MAPKs and the nuclear translocation of NF-κB p65 indicating DLE shows its anti-inflammatory activity via regulating MAPKs pathway and NF-κB pathways. And also, DLE reduced the production of reactive oxygen species in a dose-dependent manner. DLE increased HO-1 protein expression, and also increased the nuclear translocation of Nrf2. Overall, our results suggest that lettuce down-regulate various pro-inflammatory genes and have its anti-inflammatory activity via regulating MAPKs, NF-κB, and Nrf2/HO-1 pathways.

한국인 인슐린비의존형 당뇨병환자에서 엔지오텐신 1-전환효소 유전자의 다형성과 당뇨병성 만성합병증 및 관상동맥질환과의 관계

정민영,천남일,이창훈,조동혁,강호철,정동진,정명호,강정채,박종태,양승원 全南大醫大 2001 전남의대학술지 Vol.37 No.3

Impact of the GHG Reduction on Korean Economy Using Dynamic CGE

( Chung?sil Kim ),( Sang?ho Lee ) 한국농업경제학회 2006 KOREAN JOURNAL OF AGRICULTURAL ECONOMICS(KJAT) Vol.47 No.3

This study addresses on interactions of economy and environment in a dynamic computable general equilibrium (CGE) with a focus on greenhouse gas (GHG) mitigation strategy in Korea. Whether Korea has to reduce the greenhouse gas or not, it has large influence on Korea economy, such as changes in GDP and welfare. The GDP decreases by 0.113~0.132%, and the marginal abatement costs are 110~123 $US per metric ton of CO2 for Korea. In the case of reduction of greenhouse gas in Korea, the output in fossil fuel and energy.intensive sectors are decreased sharply. Therefore, we should be exempted or delayed from the international reduction programme of greenhouse gas abatement targets within the limits of the possible.

한국 초·중·고등학생들의 측두하악장애 유병상태와 악안면외상과의 관련성

정기호,권호근,김진범,최충호 대한구강보건학회 2003 大韓口腔保健學會誌 Vol.27 No.2

The purpose of this population-based epidemiologic study was to clarify the tempromandibular disorders prevalence, the maxillofacial trauma experience rate and the relation between maxillofacial truma and tempromandibular discorders in 6-14 and 16 years old korean children who were selected through the stratified random sampling method. the results are as follows; 1. The prevalence of tempromandibular clicking sounds was 1.28% in elementary students, 11.05% in middle school students, 20.07% in high school students, with an overall 6.30%. The result of the statistical test showed that girls experienced more tempromandibular clicking sounds than boys(p<0.05). 2. The prevalence of tempromandibular pain was 0.51% in elementary students, 1.95% in middle school students, 7.04% in high school students, with an overall 20.07%. 3. The prevalence in mouth opening limitation was 0.04% in elementary students, 1.36% in middle school students, 5.85% in high school students, with an overall 1.06%. 4. The percentage of treatment experience for the tempromandibular disorders was 0.00% in elementary students, 0.77% in middle school students, 2.12% in high school students, with an overall 0.46% 5. The maxillofacial trauma experience rate was 5.45% in elementary students, 9.11% in middle school students, and 11.89% in high school students, with an overall 7.22%. Males experienced more maxillofacial trauma than females(p<0.01), and urban students experienced more than rural students)p<0.05). 6. The results of the relation betwwen maxillofacial trauma and tempromandibuar disorders showed there were statistically significant realtionship between the tempromandibular experience and the clicking sound(odds ratio 1.86), and between the maxillofacial trauma and tempromandibular disorders(odds ratio 1.79).

Fatigue Crack Growth Behavior in Ultrafine Grained Low Carbon Steel

Ho,Kyung Kim,Myung-Il Choi,Chin-Sung Chung Dong-Hyuk Shin 대한기계학회 2002 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.16 No.10

Ultrafine grained (UFG) low carbon (0.15 wt.% C) steel produced by equal channel angular pressing (ECAP) was tested for investigating the effect of load ratio on the fatigue crack growth rate. Fatigue crack growth resistance and threshold of UFG steel were lower than that of as-received coarse grained steel. It was attributed to the less tortuous crack path. The UFG steel exhibited slightly higher crack growth rates and a lower △K_th with an increase of R ratio. The R ratio effect on crack growth rates and △K_th was basically indistinguishable at lower load ratio (R>0.3), compared to other alloys, which indicates that contribution of the crack closure vanishes. The crack growth rate curve for UFG steel exhibited a longer linear extension to the lower growth rate regime than that for the coarse grained as-received steel.<br/>

바나나 과실 함유탄닌이 소화효소 작용에 미치는 영향

정정한,류충호,조영수 東亞大學校附設遺傳工學硏究所 1997 遺傳工學硏究 Vol.- No.4

바나나 과실을 시중에서 구입하여 미숙과실과 황숙과실로 구별하고 이것을 과육부분과 과피부분으로 나누어서 동결건조 및 열풍건조시켜 실험 시료로서 사용하였다. 탄닌 함량을 분석한 결과 황숙과실 보다도 미숙과실에서 높았으며, 과육보다는 과피에서 열풍건조 보다는 동결건조시킨 시료에서 높은 경향을 나타내었다. 탄닌의 분획은 Sephadex LH-20칼럼을 사용하여 분획하였다. 바나나 과실에서 함유하고 있는 탄닌은 prodelphinidin, procyanidin을 구성단위로 하는 proanthocyanidin류의 축합성 탄닌으로 이러한 탄닌은 소화효소인 trypsin(EC 3. 1. 1. 3), α-amylase(EC 3. 4. 21. 4), lipase(EC 3. 2. 1. 1)에 대하여 in vitro에 있어서 저해작용을 가지고 있는 것이 확인되었으며 또한 탄닌은 중합도가 높을수록 저해율이 높다는 것이 확인되었다. It has been shown that tannins have adverse effects on growth of animals and feed utilization. Tannins are usually classified into hydrolyzable and condensed types but the adverse effects are more marked in condensed tannin in hydrolyzable tannin. Furthermore, the principle condensed tannins found in banana fruits are pro types by the polymerization of flavan-3, 4-diols either alone or in combination with other flavonoids such as catechins. Tannin of the investigated banana(Banana;Musa sapientum LINN)fruits was fractionated into four or five molecular forms, according to the degree of polymerization by chromatography on a column of Sephadex LH-20. The protein-precitating capacity of the fraction noted tannins increased in degree polymerzation. The inhibitory effect of tannins on trypsin(EC 3. 4. 21. 4), α-amylase(EC 3. 2. 1. 1) and lipase(EC 3. 1. 1. 3) activities in vitro also increased with the increased in degree of polymerization.